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O08774

- RGS12_RAT

UniProt

O08774 - RGS12_RAT

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Protein
Regulator of G-protein signaling 12
Gene
Rgs12
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.1 Publication

GO - Molecular functioni

  1. G-protein alpha-subunit binding Source: RGD
  2. GTPase activator activity Source: RGD
  3. protein binding Source: UniProtKB
  4. receptor signaling protein activity Source: InterPro

GO - Biological processi

  1. negative regulation of G-protein coupled receptor protein signaling pathway Source: RGD
  2. positive regulation of GTPase activity Source: GOC
  3. termination of G-protein coupled receptor signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 12
Short name:
RGS12
Gene namesi
Name:Rgs12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3564. Rgs12.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. apical dendrite Source: RGD
  2. cytoplasm Source: RefGenome
  3. cytosol Source: RGD
  4. neuronal cell body Source: RGD
  5. nucleus Source: RGD
  6. plasma membrane Source: RGD
  7. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13871387Regulator of G-protein signaling 12
PRO_0000204214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1115 – 1115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO08774.
PRIDEiO08774.

PTM databases

PhosphoSiteiO08774.

Expressioni

Tissue specificityi

Expressed at high levels in brain and lung and lower levels in testis, heart, and spleen.

Gene expression databases

GenevestigatoriO08774.

Interactioni

Subunit structurei

Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent.1 Publication

Protein-protein interaction databases

BioGridi248512. 10 interactions.
IntActiO08774. 3 interactions.
MINTiMINT-4582072.
STRINGi10116.ENSRNOP00000047497.

Structurei

3D structure databases

ProteinModelPortaliO08774.
SMRiO08774. Positions 705-852, 1186-1220.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 9777PDZ
Add
BLAST
Domaini227 – 339113PID
Add
BLAST
Domaini715 – 832118RGS
Add
BLAST
Domaini962 – 103271RBD 1
Add
BLAST
Domaini1034 – 110471RBD 2
Add
BLAST
Domaini1187 – 120923GoLoco
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1368 – 13736Poly-Pro

Domaini

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

Sequence similaritiesi

Contains 1 GoLoco domain.
Contains 1 PDZ (DHR) domain.
Contains 1 PID domain.
Contains 1 RGS domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG282424.
HOGENOMiHOG000049112.
HOVERGENiHBG063299.
KOiK16449.
PhylomeDBiO08774.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR003116. Raf-like_ras-bd.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: O08774-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYRAGEPGKR QSGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS     50
PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL RMVISEGSSH 100
VEPSSSDEEG GLCEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF 150
ESPSLCASGS EPLKLKQRSL SESAALRLDV GQDSLCTPHP SMLSKEEISK 200
VINDDSVFTV GLDNHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS 250
LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF 300
SAVCPDDRRF FGLVTMQTND DGCLAQEDEG ALRTSCHVFM VDPDLFHHKI 350
HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR 400
ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG 450
QGSSPGWESV SGRGSQPWSA PWNGTFCHDS EAGSPLETSP NTDRFWDLTK 500
HSGPVFHMEV PPATLRSSIP PSKRGATGSS CGFNQRWLPV HVLQEWQCGH 550
ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR 600
DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF 650
GRSRRFSLTR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS 700
CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC 750
FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP 800
DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS 850
PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRKGAFFSW 900
SRSRSTGRSQ KKKDHGDHAH DALHANGGLC RRESQGSVSS AGSLDLSEAC 950
RTSALERDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA 1000
AVDLFLVGGD KPLVLHQDSS ILATRDLRLG KRTLFRLDLV PINRSVGLKA 1050
KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI 1100
LEERDPSRGK VSTEKQKGAP VKQSSAVNSS PRNHSAMGEE RTLGKSNSIK 1150
IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN 1200
RADDQRGLLR KEDLVLPEFL RLPPGSSELA LSSPPPVKGF SKRAVTSHGQ 1250
EGAVQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH SPGSAHSTPG 1300
PPGTAQPGEK PTKPSCISTV QEGTTQAWRR LSPELEAGGI QTVEEEQVAD 1350
LTLMGEGDIS SPNSTLLPPP PLPQDTPGPT RPGTSRF 1387
Length:1,387
Mass (Da):150,469
Last modified:July 1, 1997 - v1
Checksum:i958047D106B08310
GO
Isoform 2 (identifier: O08774-2) [UniParc]FASTAAdd to Basket

Also known as: PDZ-less

The sequence of this isoform differs from the canonical sequence as follows:
     1-648: Missing.
     649-666: SFGRSRRFSLTRSLDDLE → MNLEKGLSDDSDVFIDQQ

Show »
Length:739
Mass (Da):80,135
Checksum:i792AEB5E220E1143
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 648648Missing in isoform 2.
VSP_005688Add
BLAST
Alternative sequencei649 – 66618SFGRS…LDDLE → MNLEKGLSDDSDVFIDQQ in isoform 2.
VSP_005689Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92280 mRNA. Translation: AAC53176.1.
AF035151 mRNA. Translation: AAC40154.1.
PIRiJC5502.
RefSeqiNP_062212.1. NM_019339.1. [O08774-1]
UniGeneiRn.9796.

Genome annotation databases

GeneIDi54292.
KEGGirno:54292.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92280 mRNA. Translation: AAC53176.1 .
AF035151 mRNA. Translation: AAC40154.1 .
PIRi JC5502.
RefSeqi NP_062212.1. NM_019339.1. [O08774-1 ]
UniGenei Rn.9796.

3D structure databases

ProteinModelPortali O08774.
SMRi O08774. Positions 705-852, 1186-1220.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248512. 10 interactions.
IntActi O08774. 3 interactions.
MINTi MINT-4582072.
STRINGi 10116.ENSRNOP00000047497.

PTM databases

PhosphoSitei O08774.

Proteomic databases

PaxDbi O08774.
PRIDEi O08774.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 54292.
KEGGi rno:54292.

Organism-specific databases

CTDi 6002.
RGDi 3564. Rgs12.

Phylogenomic databases

eggNOGi NOG282424.
HOGENOMi HOG000049112.
HOVERGENi HBG063299.
KOi K16449.
PhylomeDBi O08774.

Miscellaneous databases

NextBioi 610904.
PROi O08774.

Gene expression databases

Genevestigatori O08774.

Family and domain databases

Gene3Di 1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR003116. Raf-like_ras-bd.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR01301. RGSPROTEIN.
SMARTi SM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEi PS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain."
    Snow B.E., Hall R.A., Krumins A.M., Brothers G.M., Bouchard D., Brothers C.A., Chung S., Mangion J., Gilman A.G., Lefkowitz R.J., Siderovski D.P.
    J. Biol. Chem. 273:17749-17755(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Brain.
  3. "RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity."
    Kimple R.J., De Vries L., Tronchere H., Behe C.I., Morris R.A., Gist Farquhar M., Siderovski D.P.
    J. Biol. Chem. 276:29275-29281(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GNAI1; GNAI2 AND GNAI3.

Entry informationi

Entry nameiRGS12_RAT
AccessioniPrimary (citable) accession number: O08774
Secondary accession number(s): O88383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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