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O08774 (RGS12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 12

Short name=RGS12
Gene names
Name:Rgs12
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Ref.3

Subunit structure

Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent. Ref.3

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed at high levels in brain and lung and lower levels in testis, heart, and spleen.

Domain

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

Sequence similarities

Contains 1 GoLoco domain.

Contains 1 PDZ (DHR) domain.

Contains 1 PID domain.

Contains 2 RBD (Ras-binding) domains.

Contains 1 RGS domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O08774-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O08774-2)

Also known as: PDZ-less;

The sequence of this isoform differs from the canonical sequence as follows:
     1-648: Missing.
     649-666: SFGRSRRFSLTRSLDDLE → MNLEKGLSDDSDVFIDQQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13871387Regulator of G-protein signaling 12
PRO_0000204214

Regions

Domain21 – 9777PDZ
Domain227 – 339113PID
Domain715 – 832118RGS
Domain962 – 103271RBD 1
Domain1034 – 110471RBD 2
Domain1187 – 120923GoLoco
Compositional bias1368 – 13736Poly-Pro

Amino acid modifications

Cross-link1115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence1 – 648648Missing in isoform 2.
VSP_005688
Alternative sequence649 – 66618SFGRS…LDDLE → MNLEKGLSDDSDVFIDQQ in isoform 2.
VSP_005689

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 958047D106B08310

FASTA1,387150,469
        10         20         30         40         50         60 
MYRAGEPGKR QSGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS PADFVGLRAG 

        70         80         90        100        110        120 
DQILAINEIN VKKASHEDVV KLIGKCSGVL RMVISEGSSH VEPSSSDEEG GLCEGKGWLR 

       130        140        150        160        170        180 
PKLDSKALGI NRAERVVEEV QSGGIFNMIF ESPSLCASGS EPLKLKQRSL SESAALRLDV 

       190        200        210        220        230        240 
GQDSLCTPHP SMLSKEEISK VINDDSVFTV GLDNHDDFGL DASILNVAMV VGYLGSIELP 

       250        260        270        280        290        300 
STSSNLEHDS LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF 

       310        320        330        340        350        360 
SAVCPDDRRF FGLVTMQTND DGCLAQEDEG ALRTSCHVFM VDPDLFHHKI HQGIARRFGF 

       370        380        390        400        410        420 
ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR ARAFLDGDAD AHQNNSTSSN 

       430        440        450        460        470        480 
SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG QGSSPGWESV SGRGSQPWSA PWNGTFCHDS 

       490        500        510        520        530        540 
EAGSPLETSP NTDRFWDLTK HSGPVFHMEV PPATLRSSIP PSKRGATGSS CGFNQRWLPV 

       550        560        570        580        590        600 
HVLQEWQCGH ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR 

       610        620        630        640        650        660 
DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF GRSRRFSLTR 

       670        680        690        700        710        720 
SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS CRRLRERRVA SWAVSFERLL 

       730        740        750        760        770        780 
QDPVGVRYFS DFLRKEFSEE NILFWQACEC FSHVPAHDKK ELSYRAREIF SKFLCSKATT 

       790        800        810        820        830        840 
PVNIDSQAQL ADDILNAPHP DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT 

       850        860        870        880        890        900 
LPDSQQVPSS PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRKGAFFSW 

       910        920        930        940        950        960 
SRSRSTGRSQ KKKDHGDHAH DALHANGGLC RRESQGSVSS AGSLDLSEAC RTSALERDKA 

       970        980        990       1000       1010       1020 
AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA AVDLFLVGGD KPLVLHQDSS 

      1030       1040       1050       1060       1070       1080 
ILATRDLRLG KRTLFRLDLV PINRSVGLKA KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG 

      1090       1100       1110       1120       1130       1140 
EKEPLDLGAP ISSLDGQRVI LEERDPSRGK VSTEKQKGAP VKQSSAVNSS PRNHSAMGEE 

      1150       1160       1170       1180       1190       1200 
RTLGKSNSIK IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN 

      1210       1220       1230       1240       1250       1260 
RADDQRGLLR KEDLVLPEFL RLPPGSSELA LSSPPPVKGF SKRAVTSHGQ EGAVQTEESY 

      1270       1280       1290       1300       1310       1320 
SDSPATSPAS AQSPCSAYSP GSAHSPGSAH SPGSAHSTPG PPGTAQPGEK PTKPSCISTV 

      1330       1340       1350       1360       1370       1380 
QEGTTQAWRR LSPELEAGGI QTVEEEQVAD LTLMGEGDIS SPNSTLLPPP PLPQDTPGPT 


RPGTSRF 

« Hide

Isoform 2 (PDZ-less) [UniParc].

Checksum: 792AEB5E220E1143
Show »

FASTA73980,135

References

[1]"Molecular cloning and expression analysis of rat Rgs12 and Rgs14."
Snow B.E., Antonio L., Suggs S., Gutstein H.B., Siderovski D.P.
Biochem. Biophys. Res. Commun. 233:770-777(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain."
Snow B.E., Hall R.A., Krumins A.M., Brothers G.M., Bouchard D., Brothers C.A., Chung S., Mangion J., Gilman A.G., Lefkowitz R.J., Siderovski D.P.
J. Biol. Chem. 273:17749-17755(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Brain.
[3]"RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity."
Kimple R.J., De Vries L., Tronchere H., Behe C.I., Morris R.A., Gist Farquhar M., Siderovski D.P.
J. Biol. Chem. 276:29275-29281(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GNAI1; GNAI2 AND GNAI3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92280 mRNA. Translation: AAC53176.1.
AF035151 mRNA. Translation: AAC40154.1.
PIRJC5502.
RefSeqNP_062212.1. NM_019339.1. [O08774-1]
UniGeneRn.9796.

3D structure databases

ProteinModelPortalO08774.
SMRO08774. Positions 705-852, 1186-1220.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248512. 10 interactions.
IntActO08774. 3 interactions.
MINTMINT-4582072.
STRING10116.ENSRNOP00000047497.

PTM databases

PhosphoSiteO08774.

Proteomic databases

PaxDbO08774.
PRIDEO08774.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID54292.
KEGGrno:54292.

Organism-specific databases

CTD6002.
RGD3564. Rgs12.

Phylogenomic databases

eggNOGNOG282424.
HOGENOMHOG000049112.
HOVERGENHBG063299.
KOK16449.
PhylomeDBO08774.

Gene expression databases

GenevestigatorO08774.

Family and domain databases

Gene3D1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProIPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR003116. Raf-like_ras-bd.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEPS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610904.
PROO08774.

Entry information

Entry nameRGS12_RAT
AccessionPrimary (citable) accession number: O08774
Secondary accession number(s): O88383
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families