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O08774

- RGS12_RAT

UniProt

O08774 - RGS12_RAT

Protein

Regulator of G-protein signaling 12

Gene

Rgs12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.1 Publication

    GO - Molecular functioni

    1. G-protein alpha-subunit binding Source: RGD
    2. GTPase activator activity Source: RGD
    3. protein binding Source: UniProtKB
    4. receptor signaling protein activity Source: InterPro

    GO - Biological processi

    1. negative regulation of G-protein coupled receptor protein signaling pathway Source: RGD
    2. positive regulation of GTPase activity Source: GOC
    3. termination of G-protein coupled receptor signaling pathway Source: RGD

    Keywords - Molecular functioni

    Signal transduction inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of G-protein signaling 12
    Short name:
    RGS12
    Gene namesi
    Name:Rgs12
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3564. Rgs12.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. apical dendrite Source: RGD
    2. cytoplasm Source: RefGenome
    3. cytosol Source: RGD
    4. neuronal cell body Source: RGD
    5. nucleus Source: RGD
    6. plasma membrane Source: RGD
    7. protein complex Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13871387Regulator of G-protein signaling 12PRO_0000204214Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki1115 – 1115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiO08774.
    PRIDEiO08774.

    PTM databases

    PhosphoSiteiO08774.

    Expressioni

    Tissue specificityi

    Expressed at high levels in brain and lung and lower levels in testis, heart, and spleen.

    Gene expression databases

    GenevestigatoriO08774.

    Interactioni

    Subunit structurei

    Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent.1 Publication

    Protein-protein interaction databases

    BioGridi248512. 10 interactions.
    IntActiO08774. 3 interactions.
    MINTiMINT-4582072.
    STRINGi10116.ENSRNOP00000047497.

    Structurei

    3D structure databases

    ProteinModelPortaliO08774.
    SMRiO08774. Positions 705-852, 1186-1220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 9777PDZPROSITE-ProRule annotationAdd
    BLAST
    Domaini227 – 339113PIDPROSITE-ProRule annotationAdd
    BLAST
    Domaini715 – 832118RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini962 – 103271RBD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1034 – 110471RBD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1187 – 120923GoLocoPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1368 – 13736Poly-Pro

    Domaini

    The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

    Sequence similaritiesi

    Contains 1 GoLoco domain.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 PID domain.PROSITE-ProRule annotation
    Contains 2 RBD (Ras-binding) domains.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG282424.
    HOGENOMiHOG000049112.
    HOVERGENiHBG063299.
    KOiK16449.
    PhylomeDBiO08774.

    Family and domain databases

    Gene3Di1.10.196.10. 1 hit.
    2.30.29.30. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR003109. GoLoco_motif.
    IPR001478. PDZ.
    IPR011993. PH_like_dom.
    IPR006020. PTB/PI_dom.
    IPR003116. Raf-like_ras-bd.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF02188. GoLoco. 1 hit.
    PF00595. PDZ. 1 hit.
    PF02196. RBD. 2 hits.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR01301. RGSPROTEIN.
    SMARTiSM00390. GoLoco. 1 hit.
    SM00228. PDZ. 1 hit.
    SM00462. PTB. 1 hit.
    SM00455. RBD. 2 hits.
    SM00315. RGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF54236. SSF54236. 2 hits.
    PROSITEiPS50877. GOLOCO. 1 hit.
    PS50106. PDZ. 1 hit.
    PS01179. PID. 1 hit.
    PS50898. RBD. 2 hits.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: O08774-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYRAGEPGKR QSGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS     50
    PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL RMVISEGSSH 100
    VEPSSSDEEG GLCEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF 150
    ESPSLCASGS EPLKLKQRSL SESAALRLDV GQDSLCTPHP SMLSKEEISK 200
    VINDDSVFTV GLDNHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS 250
    LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF 300
    SAVCPDDRRF FGLVTMQTND DGCLAQEDEG ALRTSCHVFM VDPDLFHHKI 350
    HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR 400
    ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG 450
    QGSSPGWESV SGRGSQPWSA PWNGTFCHDS EAGSPLETSP NTDRFWDLTK 500
    HSGPVFHMEV PPATLRSSIP PSKRGATGSS CGFNQRWLPV HVLQEWQCGH 550
    ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR 600
    DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF 650
    GRSRRFSLTR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS 700
    CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC 750
    FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP 800
    DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS 850
    PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRKGAFFSW 900
    SRSRSTGRSQ KKKDHGDHAH DALHANGGLC RRESQGSVSS AGSLDLSEAC 950
    RTSALERDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA 1000
    AVDLFLVGGD KPLVLHQDSS ILATRDLRLG KRTLFRLDLV PINRSVGLKA 1050
    KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI 1100
    LEERDPSRGK VSTEKQKGAP VKQSSAVNSS PRNHSAMGEE RTLGKSNSIK 1150
    IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN 1200
    RADDQRGLLR KEDLVLPEFL RLPPGSSELA LSSPPPVKGF SKRAVTSHGQ 1250
    EGAVQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH SPGSAHSTPG 1300
    PPGTAQPGEK PTKPSCISTV QEGTTQAWRR LSPELEAGGI QTVEEEQVAD 1350
    LTLMGEGDIS SPNSTLLPPP PLPQDTPGPT RPGTSRF 1387
    Length:1,387
    Mass (Da):150,469
    Last modified:July 1, 1997 - v1
    Checksum:i958047D106B08310
    GO
    Isoform 2 (identifier: O08774-2) [UniParc]FASTAAdd to Basket

    Also known as: PDZ-less

    The sequence of this isoform differs from the canonical sequence as follows:
         1-648: Missing.
         649-666: SFGRSRRFSLTRSLDDLE → MNLEKGLSDDSDVFIDQQ

    Show »
    Length:739
    Mass (Da):80,135
    Checksum:i792AEB5E220E1143
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 648648Missing in isoform 2. CuratedVSP_005688Add
    BLAST
    Alternative sequencei649 – 66618SFGRS…LDDLE → MNLEKGLSDDSDVFIDQQ in isoform 2. CuratedVSP_005689Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92280 mRNA. Translation: AAC53176.1.
    AF035151 mRNA. Translation: AAC40154.1.
    PIRiJC5502.
    RefSeqiNP_062212.1. NM_019339.1. [O08774-1]
    UniGeneiRn.9796.

    Genome annotation databases

    GeneIDi54292.
    KEGGirno:54292.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92280 mRNA. Translation: AAC53176.1 .
    AF035151 mRNA. Translation: AAC40154.1 .
    PIRi JC5502.
    RefSeqi NP_062212.1. NM_019339.1. [O08774-1 ]
    UniGenei Rn.9796.

    3D structure databases

    ProteinModelPortali O08774.
    SMRi O08774. Positions 705-852, 1186-1220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248512. 10 interactions.
    IntActi O08774. 3 interactions.
    MINTi MINT-4582072.
    STRINGi 10116.ENSRNOP00000047497.

    PTM databases

    PhosphoSitei O08774.

    Proteomic databases

    PaxDbi O08774.
    PRIDEi O08774.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 54292.
    KEGGi rno:54292.

    Organism-specific databases

    CTDi 6002.
    RGDi 3564. Rgs12.

    Phylogenomic databases

    eggNOGi NOG282424.
    HOGENOMi HOG000049112.
    HOVERGENi HBG063299.
    KOi K16449.
    PhylomeDBi O08774.

    Miscellaneous databases

    NextBioi 610904.
    PROi O08774.

    Gene expression databases

    Genevestigatori O08774.

    Family and domain databases

    Gene3Di 1.10.196.10. 1 hit.
    2.30.29.30. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR003109. GoLoco_motif.
    IPR001478. PDZ.
    IPR011993. PH_like_dom.
    IPR006020. PTB/PI_dom.
    IPR003116. Raf-like_ras-bd.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF02188. GoLoco. 1 hit.
    PF00595. PDZ. 1 hit.
    PF02196. RBD. 2 hits.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR01301. RGSPROTEIN.
    SMARTi SM00390. GoLoco. 1 hit.
    SM00228. PDZ. 1 hit.
    SM00462. PTB. 1 hit.
    SM00455. RBD. 2 hits.
    SM00315. RGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF54236. SSF54236. 2 hits.
    PROSITEi PS50877. GOLOCO. 1 hit.
    PS50106. PDZ. 1 hit.
    PS01179. PID. 1 hit.
    PS50898. RBD. 2 hits.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain."
      Snow B.E., Hall R.A., Krumins A.M., Brothers G.M., Bouchard D., Brothers C.A., Chung S., Mangion J., Gilman A.G., Lefkowitz R.J., Siderovski D.P.
      J. Biol. Chem. 273:17749-17755(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Tissue: Brain.
    3. "RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity."
      Kimple R.J., De Vries L., Tronchere H., Behe C.I., Morris R.A., Gist Farquhar M., Siderovski D.P.
      J. Biol. Chem. 276:29275-29281(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GNAI1; GNAI2 AND GNAI3.

    Entry informationi

    Entry nameiRGS12_RAT
    AccessioniPrimary (citable) accession number: O08774
    Secondary accession number(s): O88383
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3