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O08774

- RGS12_RAT

UniProt

O08774 - RGS12_RAT

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Protein

Regulator of G-protein signaling 12

Gene

Rgs12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.1 Publication

GO - Molecular functioni

  1. G-protein alpha-subunit binding Source: RGD
  2. GTPase activator activity Source: RGD
  3. receptor signaling protein activity Source: InterPro

GO - Biological processi

  1. negative regulation of G-protein coupled receptor protein signaling pathway Source: RGD
  2. positive regulation of GTPase activity Source: GOC
  3. termination of G-protein coupled receptor signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 12
Short name:
RGS12
Gene namesi
Name:Rgs12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3564. Rgs12.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. apical dendrite Source: RGD
  2. cytoplasm Source: RefGenome
  3. cytosol Source: RGD
  4. neuronal cell body Source: RGD
  5. nucleus Source: RGD
  6. plasma membrane Source: RGD
  7. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13871387Regulator of G-protein signaling 12PRO_0000204214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1115 – 1115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO08774.
PRIDEiO08774.

PTM databases

PhosphoSiteiO08774.

Expressioni

Tissue specificityi

Expressed at high levels in brain and lung and lower levels in testis, heart, and spleen.

Gene expression databases

GenevestigatoriO08774.

Interactioni

Subunit structurei

Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent.1 Publication

Protein-protein interaction databases

BioGridi248512. 10 interactions.
IntActiO08774. 3 interactions.
MINTiMINT-4582072.
STRINGi10116.ENSRNOP00000047497.

Structurei

3D structure databases

ProteinModelPortaliO08774.
SMRiO08774. Positions 705-852, 1186-1220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 9777PDZPROSITE-ProRule annotationAdd
BLAST
Domaini227 – 339113PIDPROSITE-ProRule annotationAdd
BLAST
Domaini715 – 832118RGSPROSITE-ProRule annotationAdd
BLAST
Domaini962 – 103271RBD 1PROSITE-ProRule annotationAdd
BLAST
Domaini1034 – 110471RBD 2PROSITE-ProRule annotationAdd
BLAST
Domaini1187 – 120923GoLocoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1368 – 13736Poly-Pro

Domaini

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.

Sequence similaritiesi

Contains 1 GoLoco domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 2 RBD (Ras-binding) domains.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG282424.
HOGENOMiHOG000049112.
HOVERGENiHBG063299.
InParanoidiO08774.
KOiK16449.
PhylomeDBiO08774.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR003116. Raf-like_ras-bd.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: O08774-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRAGEPGKR QSGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS
60 70 80 90 100
PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL RMVISEGSSH
110 120 130 140 150
VEPSSSDEEG GLCEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF
160 170 180 190 200
ESPSLCASGS EPLKLKQRSL SESAALRLDV GQDSLCTPHP SMLSKEEISK
210 220 230 240 250
VINDDSVFTV GLDNHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS
260 270 280 290 300
LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF
310 320 330 340 350
SAVCPDDRRF FGLVTMQTND DGCLAQEDEG ALRTSCHVFM VDPDLFHHKI
360 370 380 390 400
HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR
410 420 430 440 450
ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG
460 470 480 490 500
QGSSPGWESV SGRGSQPWSA PWNGTFCHDS EAGSPLETSP NTDRFWDLTK
510 520 530 540 550
HSGPVFHMEV PPATLRSSIP PSKRGATGSS CGFNQRWLPV HVLQEWQCGH
560 570 580 590 600
ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR
610 620 630 640 650
DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF
660 670 680 690 700
GRSRRFSLTR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS
710 720 730 740 750
CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC
760 770 780 790 800
FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP
810 820 830 840 850
DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS
860 870 880 890 900
PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRKGAFFSW
910 920 930 940 950
SRSRSTGRSQ KKKDHGDHAH DALHANGGLC RRESQGSVSS AGSLDLSEAC
960 970 980 990 1000
RTSALERDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA
1010 1020 1030 1040 1050
AVDLFLVGGD KPLVLHQDSS ILATRDLRLG KRTLFRLDLV PINRSVGLKA
1060 1070 1080 1090 1100
KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI
1110 1120 1130 1140 1150
LEERDPSRGK VSTEKQKGAP VKQSSAVNSS PRNHSAMGEE RTLGKSNSIK
1160 1170 1180 1190 1200
IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN
1210 1220 1230 1240 1250
RADDQRGLLR KEDLVLPEFL RLPPGSSELA LSSPPPVKGF SKRAVTSHGQ
1260 1270 1280 1290 1300
EGAVQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH SPGSAHSTPG
1310 1320 1330 1340 1350
PPGTAQPGEK PTKPSCISTV QEGTTQAWRR LSPELEAGGI QTVEEEQVAD
1360 1370 1380
LTLMGEGDIS SPNSTLLPPP PLPQDTPGPT RPGTSRF
Length:1,387
Mass (Da):150,469
Last modified:July 1, 1997 - v1
Checksum:i958047D106B08310
GO
Isoform 2 (identifier: O08774-2) [UniParc]FASTAAdd to Basket

Also known as: PDZ-less

The sequence of this isoform differs from the canonical sequence as follows:
     1-648: Missing.
     649-666: SFGRSRRFSLTRSLDDLE → MNLEKGLSDDSDVFIDQQ

Show »
Length:739
Mass (Da):80,135
Checksum:i792AEB5E220E1143
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 648648Missing in isoform 2. CuratedVSP_005688Add
BLAST
Alternative sequencei649 – 66618SFGRS…LDDLE → MNLEKGLSDDSDVFIDQQ in isoform 2. CuratedVSP_005689Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92280 mRNA. Translation: AAC53176.1.
AF035151 mRNA. Translation: AAC40154.1.
PIRiJC5502.
RefSeqiNP_062212.1. NM_019339.1. [O08774-1]
UniGeneiRn.9796.

Genome annotation databases

GeneIDi54292.
KEGGirno:54292.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92280 mRNA. Translation: AAC53176.1 .
AF035151 mRNA. Translation: AAC40154.1 .
PIRi JC5502.
RefSeqi NP_062212.1. NM_019339.1. [O08774-1 ]
UniGenei Rn.9796.

3D structure databases

ProteinModelPortali O08774.
SMRi O08774. Positions 705-852, 1186-1220.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248512. 10 interactions.
IntActi O08774. 3 interactions.
MINTi MINT-4582072.
STRINGi 10116.ENSRNOP00000047497.

PTM databases

PhosphoSitei O08774.

Proteomic databases

PaxDbi O08774.
PRIDEi O08774.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 54292.
KEGGi rno:54292.

Organism-specific databases

CTDi 6002.
RGDi 3564. Rgs12.

Phylogenomic databases

eggNOGi NOG282424.
HOGENOMi HOG000049112.
HOVERGENi HBG063299.
InParanoidi O08774.
KOi K16449.
PhylomeDBi O08774.

Miscellaneous databases

NextBioi 610904.
PROi O08774.

Gene expression databases

Genevestigatori O08774.

Family and domain databases

Gene3Di 1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
IPR003116. Raf-like_ras-bd.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR01301. RGSPROTEIN.
SMARTi SM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEi PS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain."
    Snow B.E., Hall R.A., Krumins A.M., Brothers G.M., Bouchard D., Brothers C.A., Chung S., Mangion J., Gilman A.G., Lefkowitz R.J., Siderovski D.P.
    J. Biol. Chem. 273:17749-17755(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Brain.
  3. "RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity."
    Kimple R.J., De Vries L., Tronchere H., Behe C.I., Morris R.A., Gist Farquhar M., Siderovski D.P.
    J. Biol. Chem. 276:29275-29281(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GNAI1; GNAI2 AND GNAI3.

Entry informationi

Entry nameiRGS12_RAT
AccessioniPrimary (citable) accession number: O08774
Secondary accession number(s): O88383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3