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Protein

Neurotrypsin

Gene

Prss12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei516 – 5172Reactive bond homologSequence Analysis
Active sitei562 – 5621Charge relay system
Active sitei612 – 6121Charge relay system
Active sitei711 – 7111Charge relay system

GO - Molecular functioni

  • peptidase activity Source: MGI
  • scavenger receptor activity Source: InterPro
  • serine-type endopeptidase activity Source: MGI

GO - Biological processi

  • exocytosis Source: UniProtKB
  • proteolysis Source: MGI
  • zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.237.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotrypsin (EC:3.4.21.-)
Alternative name(s):
Brain-specific serine protease 3
Short name:
BSSP-3
Motopsin
Serine protease 12
Gene namesi
Name:Prss12
Synonyms:Bssp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1100881. Prss12.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasmic vesicle Source: MGI
  • dendrite Source: MGI
  • extracellular region Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • synapse Source: MGI
  • synaptic cleft Source: MGI
  • terminal bouton Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 761740NeurotrypsinPRO_0000027670Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi85 ↔ 157By similarity
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi101 ↔ 141By similarity
Disulfide bondi130 ↔ 155By similarity
Disulfide bondi191 ↔ 255By similarity
Disulfide bondi204 ↔ 265By similarity
Disulfide bondi235 ↔ 245By similarity
Disulfide bondi298 ↔ 361By similarity
Disulfide bondi311 ↔ 371By similarity
Disulfide bondi341 ↔ 351By similarity
Disulfide bondi411 ↔ 475By similarity
Disulfide bondi424 ↔ 485By similarity
Disulfide bondi455 ↔ 465By similarity
Disulfide bondi505 ↔ 636Sequence Analysis
Glycosylationi521 – 5211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi547 ↔ 563By similarity
Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi651 ↔ 717By similarity
Disulfide bondi680 ↔ 694By similarity
Disulfide bondi707 ↔ 736By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08762.
PaxDbiO08762.
PRIDEiO08762.

PTM databases

PhosphoSiteiO08762.

Expressioni

Tissue specificityi

Most abundant in cerebral cortex, hippocampus and amygdala.

Gene expression databases

BgeeiO08762.
CleanExiMM_PRSS12.
GenevisibleiO08762. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029603.

Structurei

3D structure databases

ProteinModelPortaliO08762.
SMRiO08762. Positions 84-160, 384-760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 15773KringlePROSITE-ProRule annotationAdd
BLAST
Domaini166 – 267102SRCR 1PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 373101SRCR 2PROSITE-ProRule annotationAdd
BLAST
Domaini386 – 487102SRCR 3PROSITE-ProRule annotationAdd
BLAST
Domaini517 – 760244Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni505 – 51612Zymogen activation regionAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 3 SRCR domains.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118953.
HOGENOMiHOG000113767.
HOVERGENiHBG006465.
InParanoidiO08762.
KOiK09624.
OMAiRYGNSTR.
OrthoDBiEOG7RNK07.
PhylomeDBiO08762.
TreeFamiTF329295.

Family and domain databases

Gene3Di3.10.250.10. 3 hits.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00530. SRCR. 3 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
PR00258. SPERACTRCPTR.
SMARTiSM00130. KR. 1 hit.
SM00202. SR. 3 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 3 hits.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALARCVLAV ILGALSVVAR ADPVSRSPLH RPHPSPPRSQ HAHYLPSSRR
60 70 80 90 100
PPRTPRFPLP LRIPAAQRPQ VLSTGHTPPT IPRRCGAGES WGNATNLGVP
110 120 130 140 150
CLHWDEVPPF LERSPPASWA ELRGQPHNFC RSPDGSGRPW CFYRNAQGKV
160 170 180 190 200
DWGYCDCGQG PALPVIRLVG GNSGHEGRVE LYHAGQWGTI CDDQWDNADA
210 220 230 240 250
DVICRQLGLS GIAKAWHQAH FGEGSGPILL DEVRCTGNEL SIEQCPKSSW
260 270 280 290 300
GEHNCGHKED AGVSCVPLTD GVIRLAGGKS THEGRLEVYY KGQWGTVCDD
310 320 330 340 350
GWTEMNTYVA CRLLGFKYGK QSSVNHFDGS NRPIWLDDVS CSGKEVSFIQ
360 370 380 390 400
CSRRQWGRHD CSHREDVGLT CYPDSDGHRL SPGFPIRLVD GENKKEGRVE
410 420 430 440 450
VFVNGQWGTI CDDGWTDKHA AVICRQLGYK GPARARTMAY FGEGKGPIHM
460 470 480 490 500
DNVKCTGNEK ALADCVKQDI GRHNCRHSED AGVICDYLEK KASSSGNKEM
510 520 530 540 550
LSSGCGLRLL HRRQKRIIGG NNSLRGAWPW QASLRLRSAH GDGRLLCGAT
560 570 580 590 600
LLSSCWVLTA AHCFKRYGNN SRSYAVRVGD YHTLVPEEFE QEIGVQQIVI
610 620 630 640 650
HRNYRPDRSD YDIALVRLQG PGEQCARLST HVLPACLPLW RERPQKTASN
660 670 680 690 700
CHITGWGDTG RAYSRTLQQA AVPLLPKRFC KERYKGLFTG RMLCAGNLQE
710 720 730 740 750
DNRVDSCQGD SGGPLMCEKP DESWVVYGVT SWGYGCGVKD TPGVYTRVPA
760
FVPWIKSVTS L
Length:761
Mass (Da):84,118
Last modified:July 1, 1997 - v1
Checksum:iDF507B03712164E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13192 mRNA. Translation: CAA73646.1.
D89871 mRNA. Translation: BAA23986.1.
BC031429 mRNA. Translation: AAH31429.1.
CCDSiCCDS17817.1.
PIRiJC5759.
RefSeqiNP_032965.1. NM_008939.2.
UniGeneiMm.9431.

Genome annotation databases

EnsembliENSMUST00000029603; ENSMUSP00000029603; ENSMUSG00000027978.
GeneIDi19142.
KEGGimmu:19142.
UCSCiuc008rfl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13192 mRNA. Translation: CAA73646.1.
D89871 mRNA. Translation: BAA23986.1.
BC031429 mRNA. Translation: AAH31429.1.
CCDSiCCDS17817.1.
PIRiJC5759.
RefSeqiNP_032965.1. NM_008939.2.
UniGeneiMm.9431.

3D structure databases

ProteinModelPortaliO08762.
SMRiO08762. Positions 84-160, 384-760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029603.

Protein family/group databases

MEROPSiS01.237.

PTM databases

PhosphoSiteiO08762.

Proteomic databases

MaxQBiO08762.
PaxDbiO08762.
PRIDEiO08762.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029603; ENSMUSP00000029603; ENSMUSG00000027978.
GeneIDi19142.
KEGGimmu:19142.
UCSCiuc008rfl.2. mouse.

Organism-specific databases

CTDi8492.
MGIiMGI:1100881. Prss12.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118953.
HOGENOMiHOG000113767.
HOVERGENiHBG006465.
InParanoidiO08762.
KOiK09624.
OMAiRYGNSTR.
OrthoDBiEOG7RNK07.
PhylomeDBiO08762.
TreeFamiTF329295.

Miscellaneous databases

NextBioi295774.
PROiO08762.
SOURCEiSearch...

Gene expression databases

BgeeiO08762.
CleanExiMM_PRSS12.
GenevisibleiO08762. MM.

Family and domain databases

Gene3Di3.10.250.10. 3 hits.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00530. SRCR. 3 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
PR00258. SPERACTRCPTR.
SMARTiSM00130. KR. 1 hit.
SM00202. SR. 3 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 3 hits.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Neurotrypsin, a novel multidomain serine protease expressed in the nervous system."
    Gschwend T.P., Krueger S.R., Kozlov S.V., Wolfer D.P., Sonderegger P.
    Mol. Cell. Neurosci. 9:207-219(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Molecular cloning of a novel brain-specific serine protease with a kringle-like structure and three scavenger receptor cysteine-rich motifs."
    Yamamura Y., Yamashiro K., Tsuruoka N., Nakazato H., Tsujimura A., Yamaguchi N.
    Biochem. Biophys. Res. Commun. 239:386-392(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiNETR_MOUSE
AccessioniPrimary (citable) accession number: O08762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: July 22, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.