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Protein

N-glycosylase/DNA lyase

Gene

Ogg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149DNABy similarity1
Binding sitei154DNABy similarity1
Binding sitei204DNABy similarity1
Active sitei249Schiff-base intermediate with DNABy similarity1
Binding sitei2668-oxoguanine; via carbonyl oxygenBy similarity1
Binding sitei2688-oxoguanineBy similarity1
Binding sitei270DNABy similarity1
Binding sitei287DNABy similarity1
Binding sitei3158-oxoguanineBy similarity1
Binding sitei3198-oxoguanineBy similarity1

GO - Molecular functioni

  • 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity Source: MGI
  • damaged DNA binding Source: MGI
  • DNA binding Source: MGI
  • DNA N-glycosylase activity Source: MGI
  • microtubule binding Source: MGI
  • oxidized base lesion DNA N-glycosylase activity Source: MGI
  • oxidized purine DNA binding Source: MGI
  • oxidized purine nucleobase lesion DNA N-glycosylase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-110329. Cleavage of the damaged pyrimidine.
R-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.
R-MMU-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Including the following 2 domains:
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Name:Ogg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1097693. Ogg1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nuclear matrix Source: UniProtKB
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585921 – 345N-glycosylase/DNA lyaseAdd BLAST345

Proteomic databases

EPDiO08760.
PaxDbiO08760.
PeptideAtlasiO08760.
PRIDEiO08760.

PTM databases

PhosphoSitePlusiO08760.

Expressioni

Tissue specificityi

Highest expression in testis.

Gene expression databases

BgeeiENSMUSG00000030271.
CleanExiMM_OGG1.
ExpressionAtlasiO08760. baseline and differential.
GenevisibleiO08760. MM.

Interactioni

GO - Molecular functioni

  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi201906. 2 interactors.
IntActiO08760. 2 interactors.
STRINGi10090.ENSMUSP00000032406.

Structurei

3D structure databases

ProteinModelPortaliO08760.
SMRiO08760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type-1 OGG1 family.Curated

Phylogenomic databases

eggNOGiKOG2875. Eukaryota.
COG0122. LUCA.
GeneTreeiENSGT00640000091554.
HOVERGENiHBG001047.
InParanoidiO08760.
KOiK03660.
OMAiFFRNLWG.
OrthoDBiEOG091G0AU6.
PhylomeDBiO08760.
TreeFamiTF323702.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg1.
IPR012904. OGG_N.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00588. ogg. 1 hit.

Sequencei

Sequence statusi: Complete.

O08760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFRSWLPSS MRHRTLSSSP ALWASIPCPR SELRLDLVLA SGQSFRWKEQ
60 70 80 90 100
SPAHWSGVLA DQVWTLTQTE DQLYCTVYRG DDSQVSRPTL EELETLHKYF
110 120 130 140 150
QLDVSLAQLY SHWASVDSHF QRVAQKFQGV RLLRQDPTEC LFSFICSSNN
160 170 180 190 200
NIARITGMVE RLCQAFGPRL IQLDDVTYHG FPNLHALAGP EAETHLRKLG
210 220 230 240 250
LGYRARYVRA SAKAILEEQG GPAWLQQLRV APYEEAHKAL CTLPGVGAKV
260 270 280 290 300
ADCICLMALD KPQAVPVDVH VWQIAHRDYG WHPKTSQAKG PSPLANKELG
310 320 330 340
NFFRNLWGPY AGWAQAVLFS ADLRQPSLSR EPPAKRKKGS KRPEG
Length:345
Mass (Da):38,883
Last modified:December 1, 2000 - v2
Checksum:i75BB0DDB084E4947
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10S → Q in AAB61289 (PubMed:9187114).Curated1
Sequence conflicti23 – 25WAS → SVA in AAB61289 (PubMed:9187114).Curated3
Sequence conflicti239A → G in AAB61289 (PubMed:9187114).Curated1
Sequence conflicti299L → S in CAA73883 (Ref. 6) Curated1
Sequence conflicti329S → F in AAB81133 (PubMed:9207108).Curated1
Sequence conflicti336R → H in CAA73883 (Ref. 6) Curated1
Sequence conflicti336R → H in CAB65240 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003596 mRNA. Translation: AAB61289.1.
Y11247 mRNA. Translation: CAA72117.1.
U88621 mRNA. Translation: AAB68616.1.
U96711 mRNA. Translation: AAB81133.1.
AF000669 mRNA. Translation: AAB63151.1.
AF012916
, AF012912, AF012913, AF012914, AF012915 Genomic DNA. Translation: AAB94512.1.
Y13479 mRNA. Translation: CAA73883.1.
AJ001307 Genomic DNA. Translation: CAB65240.1.
CCDSiCCDS20414.1.
PIRiT46962.
RefSeqiNP_035087.3. NM_010957.4.
UniGeneiMm.43612.

Genome annotation databases

EnsembliENSMUST00000032406; ENSMUSP00000032406; ENSMUSG00000030271.
GeneIDi18294.
KEGGimmu:18294.
UCSCiuc009dfh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003596 mRNA. Translation: AAB61289.1.
Y11247 mRNA. Translation: CAA72117.1.
U88621 mRNA. Translation: AAB68616.1.
U96711 mRNA. Translation: AAB81133.1.
AF000669 mRNA. Translation: AAB63151.1.
AF012916
, AF012912, AF012913, AF012914, AF012915 Genomic DNA. Translation: AAB94512.1.
Y13479 mRNA. Translation: CAA73883.1.
AJ001307 Genomic DNA. Translation: CAB65240.1.
CCDSiCCDS20414.1.
PIRiT46962.
RefSeqiNP_035087.3. NM_010957.4.
UniGeneiMm.43612.

3D structure databases

ProteinModelPortaliO08760.
SMRiO08760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201906. 2 interactors.
IntActiO08760. 2 interactors.
STRINGi10090.ENSMUSP00000032406.

PTM databases

PhosphoSitePlusiO08760.

Proteomic databases

EPDiO08760.
PaxDbiO08760.
PeptideAtlasiO08760.
PRIDEiO08760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032406; ENSMUSP00000032406; ENSMUSG00000030271.
GeneIDi18294.
KEGGimmu:18294.
UCSCiuc009dfh.2. mouse.

Organism-specific databases

CTDi4968.
MGIiMGI:1097693. Ogg1.

Phylogenomic databases

eggNOGiKOG2875. Eukaryota.
COG0122. LUCA.
GeneTreeiENSGT00640000091554.
HOVERGENiHBG001047.
InParanoidiO08760.
KOiK03660.
OMAiFFRNLWG.
OrthoDBiEOG091G0AU6.
PhylomeDBiO08760.
TreeFamiTF323702.

Enzyme and pathway databases

ReactomeiR-MMU-110329. Cleavage of the damaged pyrimidine.
R-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.
R-MMU-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.

Miscellaneous databases

PROiO08760.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030271.
CleanExiMM_OGG1.
ExpressionAtlasiO08760. baseline and differential.
GenevisibleiO08760. MM.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg1.
IPR012904. OGG_N.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00588. ogg. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOGG1_MOUSE
AccessioniPrimary (citable) accession number: O08760
Secondary accession number(s): O08733
, O08910, O08991, O35617, O35915, Q9QXE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.