O08756 (HCD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxyacyl-CoA dehydrogenase type-2
EC=1.1.1.35

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 10
Short name=17-beta-HSD 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
EC=1.1.1.178
3-hydroxyacyl-CoA dehydrogenase type II
Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
Mitochondrial ribonuclease P protein 2
Short name=Mitochondrial RNase P protein 2
Type II HADH

Gene names

Name:	Hsd17b10
Synonyms:	Erab, Hadh2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends By similarity.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH.
Subunit structure	Homotetramer. Interacts with MRPP1/TRMT10C and MRPP3/KIAA0391 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence caution	The sequence AAB57689.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 15962010. Source: MGI mitochondrial inner membrane Inferred from direct assay PubMed 12865426. Source: MGI
Molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC 3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 261	260	3-hydroxyacyl-CoA dehydrogenase type-2		PRO_0000054811
Regions
	Nucleotide binding	12 – 37	26	NAD By similarity
Sites
	Active site	168	1	Proton acceptor By similarity
	Binding site	155	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O08756 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 61213B13E2839D41	FASTA	261	27,419
	10 20 30 40 50 60 MAAAVRSVKG LVAVVTGGAS GPWLATAKRL VGQGATAVLL DVPDSEGESQ AKKLGESCIF 70 80 90 100 110 120 APANVTSEKE IQAALTLAKE KFGRIDVAVN CAGIAVAIKT YHQKKNKIHT LEDFQRVINV 130 140 150 160 170 180 NLIGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIDGMTL 190 200 210 220 230 240 PIARDLAPTG IRVVTIAPGL FATPLLTTLP EKVRNFLASQ VPFPSRLGDP AEYAHLVQTI 250 260 IENPFLNGEV IRLDGAIRMQ P

References

[1]	Fu J., Chen X., Stern D., Yan S.D. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA.
[2]	Bienvenut W.V. Submitted (JUL-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-6; 193-212 AND 215-226, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Liver.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 70-79 AND 131-147, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U96116 mRNA. Translation: AAB57689.1. Different initiation.
IPI	IPI00320847.
UniGene	Mm.6994.
3D structure databases
ProteinModelPortal	O08756.
SMR	O08756. Positions 7-261.
ModBase	Search...
PTM databases
PhosphoSite	O08756.
2D gel databases
SWISS-2DPAGE	O08756.
Proteomic databases
PaxDb	O08756.
PRIDE	O08756.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1333871. Hsd17b10.
Phylogenomic databases
eggNOG	COG1028.
HOVERGEN	HBG002145.
OrthoDB	EOG46MBKC.
Gene expression databases
CleanEx	MM_HSD17B10.
Genevestigator	O08756.
GermOnline	ENSMUSG00000025260. Mus musculus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HSD17B10. mouse.
PMAP-CutDB	O08756.
SOURCE	Search...

Entry information

Entry name

HCD2_MOUSE

Accession

Primary (citable) accession number: O08756

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 110 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program