Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O08750 (NFIL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor interleukin-3-regulated protein
Alternative name(s):
E4 promoter-binding protein 4
Embryo implantation-related NFIL3/E4BP4-like transcription factor
Gene names
Name:Nfil3
Synonyms:E4bp4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites By similarity. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells By similarity. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Ref.1 Ref.5 Ref.7 Ref.9

Subunit structure

Homodimer By similarity. Binds DNA as a dimer By similarity. Interacts with DR1 By similarity. Interacts with PER2 and CRY2. Ref.8

Subcellular location

Nucleus Ref.5.

Tissue specificity

Expressed in suprachiasmatic nucleus and liver (at protein level). Expressed in suprachiasmatic nucleus, hippocampus, gyrus dentatus, piriform cortex, internal granular layer of olfactory bulb, dorsomedial hypothalamic nucleus, pontine nuclei, granular layer of cerebellum, liver and calvariae osteoblasts. Ref.5 Ref.6

Induction

Expression is regulated by circadian rhythms. Up-regulated by parathyroid hormone (PTH) (at protein level). Up-regulated by IL-3, forskolin, 8-bromo-cAMP, phorbol myristate acetate and PTH in primary osteoblasts and calvariae. Ref.1 Ref.6 Ref.7

Sequence similarities

Belongs to the bZIP family. NFIL3 subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Nuclear factor interleukin-3-regulated protein
PRO_0000292668

Regions

Domain73 – 13664bZIP
Region79 – 9517Basic motif By similarity
Region99 – 1068Leucine-zipper By similarity
Region281 – 420140Necessary for transcriptional repression and sufficient for interaction with PER2

Amino acid modifications

Modified residue3011Phosphoserine By similarity
Modified residue3531Phosphoserine By similarity

Experimental info

Sequence conflict1011L → F in BAE26446. Ref.3
Sequence conflict1411L → F in AAI00385. Ref.4
Sequence conflict1891D → G in AAI00385. Ref.4
Sequence conflict4501F → V in BAE26446. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O08750 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: DC711EB4CAC0112E

FASTA46250,943
        10         20         30         40         50         60 
MQLRKMQTIK KEPAPLDPTS SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG SMGKNKSSAC 

        70         80         90        100        110        120 
RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV LENKLIALGE ENATLKAELL 

       130        140        150        160        170        180 
SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ DYQTSKAAVS SFVDEHEPAM VAGSCISVIK 

       190        200        210        220        230        240 
HSPQSSLSDV SEVSSVEHTQ ESPAQGGCRS PENKFPVIKQ EPVELESFAR EAREERGTYS 

       250        260        270        280        290        300 
TSIYQSYMGS SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH 

       310        320        330        340        350        360 
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK LSSPADAIAK 

       370        380        390        400        410        420 
RHFDLEKHGT SGMAHSSLPP FSVQVTNIQD WSLKSEHWHH KELSSKTQSS FKTGVVEVKD 

       430        440        450        460 
GGYKVSEAEN LYLKQGIANL SAEVVSLKRF IATQPISASD SR 

« Hide

References

« Hide 'large scale' references
[1]"Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes."
Ikushima S., Inukai T., Inaba T., Nimer S.D., Cleveland J.L., Look A.T.
Proc. Natl. Acad. Sci. U.S.A. 94:2609-2614(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DNA-BINDING.
[2]"Identification of novel endometrial factors involved in the mouse embryo implantation."
Shen Q.-X., Wang J., Huang Z.-P.
Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Embryo and Uterus.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and FVB/N.
Tissue: Egg and Mammary tumor.
[5]"Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism."
Mitsui S., Yamaguchi S., Matsuo T., Ishida Y., Okamura H.
Genes Dev. 15:995-1006(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Parathyroid hormone-induced E4BP4/NFIL3 down-regulates transcription in osteoblasts."
Ozkurt I.C., Tetradis S.
J. Biol. Chem. 278:26803-26809(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
[7]"Parathyroid hormone induces E4bp4 messenger ribonucleic acid expression primarily through cyclic adenosine 3',5'-monophosphate signaling in osteoblasts."
Ozkurt I.C., Pirih F.Q., Tetradis S.
Endocrinology 145:3696-3703(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, DNA-BINDING.
[8]"The negative transcription factor E4BP4 is associated with circadian clock protein PERIOD2."
Ohno T., Onishi Y., Ishida N.
Biochem. Biophys. Res. Commun. 354:1010-1015(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PER2 AND CRY2.
[9]"A novel E4BP4 element drives circadian expression of mPeriod2."
Ohno T., Onishi Y., Ishida N.
Nucleic Acids Res. 35:648-655(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[10]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U83148 Genomic DNA. Translation: AAB53973.1.
AY061760 mRNA. Translation: AAL29942.1.
AK145451 mRNA. Translation: BAE26446.1.
BC031377 mRNA. Translation: AAH31377.1.
BC100384 mRNA. Translation: AAI00385.1.
CCDSCCDS26519.1.
RefSeqNP_059069.1. NM_017373.3.
XP_006516938.1. XM_006516875.1.
XP_006516939.1. XM_006516876.1.
XP_006516940.1. XM_006516877.1.
XP_006516941.1. XM_006516878.1.
XP_006516942.1. XM_006516879.1.
UniGeneMm.136604.

3D structure databases

ProteinModelPortalO08750.
SMRO08750. Positions 41-124.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO08750.

Proteomic databases

PRIDEO08750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000071065; ENSMUSP00000065363; ENSMUSG00000056749.
GeneID18030.
KEGGmmu:18030.
UCSCuc007qnh.1. mouse.

Organism-specific databases

CTD4783.
MGIMGI:109495. Nfil3.

Phylogenomic databases

eggNOGNOG260819.
GeneTreeENSGT00530000064087.
HOGENOMHOG000059596.
HOVERGENHBG105717.
InParanoidO08750.
KOK09059.
OMAWHQKELN.
OrthoDBEOG7GQXWM.
PhylomeDBO08750.
TreeFamTF328374.

Gene expression databases

BgeeO08750.
CleanExMM_NFIL3.
GenevestigatorO08750.

Family and domain databases

InterProIPR004827. bZIP.
IPR016743. TF_bZIP_E4BP4.
IPR010533. Vert_IL3-reg_TF.
[Graphical view]
PfamPF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
[Graphical view]
PIRSFPIRSF019029. bZIP_E4BP4. 1 hit.
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293109.
PROO08750.
SOURCESearch...

Entry information

Entry nameNFIL3_MOUSE
AccessionPrimary (citable) accession number: O08750
Secondary accession number(s): Q3ULK2, Q497U0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot