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O08750

- NFIL3_MOUSE

UniProt

O08750 - NFIL3_MOUSE

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Protein

Nuclear factor interleukin-3-regulated protein

Gene
Nfil3, E4bp4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites By similarity. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells By similarity. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death.4 Publications

GO - Molecular functioni

  1. protein binding Source: BHF-UCL
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: Ensembl
  3. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI

GO - Biological processi

  1. cellular response to interleukin-4 Source: MGI
  2. circadian rhythm Source: InterPro
  3. positive regulation of gene expression Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor interleukin-3-regulated protein
Alternative name(s):
E4 promoter-binding protein 4
Embryo implantation-related NFIL3/E4BP4-like transcription factor
Gene namesi
Name:Nfil3
Synonyms:E4bp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:109495. Nfil3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Nuclear factor interleukin-3-regulated proteinPRO_0000292668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 3011Phosphoserine By similarity
Modified residuei353 – 3531Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO08750.

PTM databases

PhosphoSiteiO08750.

Expressioni

Tissue specificityi

Expressed in suprachiasmatic nucleus and liver (at protein level). Expressed in suprachiasmatic nucleus, hippocampus, gyrus dentatus, piriform cortex, internal granular layer of olfactory bulb, dorsomedial hypothalamic nucleus, pontine nuclei, granular layer of cerebellum, liver and calvariae osteoblasts.2 Publications

Inductioni

Expression is regulated by circadian rhythms. Up-regulated by parathyroid hormone (PTH) (at protein level). Up-regulated by IL-3, forskolin, 8-bromo-cAMP, phorbol myristate acetate and PTH in primary osteoblasts and calvariae.3 Publications

Gene expression databases

BgeeiO08750.
CleanExiMM_NFIL3.
GenevestigatoriO08750.

Interactioni

Subunit structurei

Homodimer By similarity. Binds DNA as a dimer By similarity. Interacts with DR1 By similarity. Interacts with PER2 and CRY2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO08750.
SMRiO08750. Positions 41-124.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 13664bZIPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 9517Basic motif By similarityAdd
BLAST
Regioni99 – 1068Leucine-zipper By similarity
Regioni281 – 420140Necessary for transcriptional repression and sufficient for interaction with PER2Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. NFIL3 subfamily.

Phylogenomic databases

eggNOGiNOG260819.
GeneTreeiENSGT00530000064087.
HOGENOMiHOG000059596.
HOVERGENiHBG105717.
InParanoidiO08750.
KOiK09059.
OMAiWHQKELN.
OrthoDBiEOG7GQXWM.
PhylomeDBiO08750.
TreeFamiTF328374.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016743. TF_bZIP_E4BP4.
IPR010533. Vert_IL3-reg_TF.
[Graphical view]
PfamiPF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
[Graphical view]
PIRSFiPIRSF019029. bZIP_E4BP4. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08750-1 [UniParc]FASTAAdd to Basket

« Hide

MQLRKMQTIK KEPAPLDPTS SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG    50
SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV 100
LENKLIALGE ENATLKAELL SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ 150
DYQTSKAAVS SFVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ 200
ESPAQGGCRS PENKFPVIKQ EPVELESFAR EAREERGTYS TSIYQSYMGS 250
SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH 300
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK 350
LSSPADAIAK RHFDLEKHGT SGMAHSSLPP FSVQVTNIQD WSLKSEHWHH 400
KELSSKTQSS FKTGVVEVKD GGYKVSEAEN LYLKQGIANL SAEVVSLKRF 450
IATQPISASD SR 462
Length:462
Mass (Da):50,943
Last modified:July 1, 1997 - v1
Checksum:iDC711EB4CAC0112E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011L → F in BAE26446. 1 Publication
Sequence conflicti141 – 1411L → F in AAI00385. 1 Publication
Sequence conflicti189 – 1891D → G in AAI00385. 1 Publication
Sequence conflicti450 – 4501F → V in BAE26446. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83148 Genomic DNA. Translation: AAB53973.1.
AY061760 mRNA. Translation: AAL29942.1.
AK145451 mRNA. Translation: BAE26446.1.
BC031377 mRNA. Translation: AAH31377.1.
BC100384 mRNA. Translation: AAI00385.1.
CCDSiCCDS26519.1.
RefSeqiNP_059069.1. NM_017373.3.
XP_006516938.1. XM_006516875.1.
XP_006516939.1. XM_006516876.1.
XP_006516940.1. XM_006516877.1.
XP_006516941.1. XM_006516878.1.
XP_006516942.1. XM_006516879.1.
UniGeneiMm.136604.

Genome annotation databases

EnsembliENSMUST00000071065; ENSMUSP00000065363; ENSMUSG00000056749.
GeneIDi18030.
KEGGimmu:18030.
UCSCiuc007qnh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83148 Genomic DNA. Translation: AAB53973.1 .
AY061760 mRNA. Translation: AAL29942.1 .
AK145451 mRNA. Translation: BAE26446.1 .
BC031377 mRNA. Translation: AAH31377.1 .
BC100384 mRNA. Translation: AAI00385.1 .
CCDSi CCDS26519.1.
RefSeqi NP_059069.1. NM_017373.3.
XP_006516938.1. XM_006516875.1.
XP_006516939.1. XM_006516876.1.
XP_006516940.1. XM_006516877.1.
XP_006516941.1. XM_006516878.1.
XP_006516942.1. XM_006516879.1.
UniGenei Mm.136604.

3D structure databases

ProteinModelPortali O08750.
SMRi O08750. Positions 41-124.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O08750.

Proteomic databases

PRIDEi O08750.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000071065 ; ENSMUSP00000065363 ; ENSMUSG00000056749 .
GeneIDi 18030.
KEGGi mmu:18030.
UCSCi uc007qnh.1. mouse.

Organism-specific databases

CTDi 4783.
MGIi MGI:109495. Nfil3.

Phylogenomic databases

eggNOGi NOG260819.
GeneTreei ENSGT00530000064087.
HOGENOMi HOG000059596.
HOVERGENi HBG105717.
InParanoidi O08750.
KOi K09059.
OMAi WHQKELN.
OrthoDBi EOG7GQXWM.
PhylomeDBi O08750.
TreeFami TF328374.

Miscellaneous databases

NextBioi 293109.
PROi O08750.
SOURCEi Search...

Gene expression databases

Bgeei O08750.
CleanExi MM_NFIL3.
Genevestigatori O08750.

Family and domain databases

InterProi IPR004827. bZIP.
IPR016743. TF_bZIP_E4BP4.
IPR010533. Vert_IL3-reg_TF.
[Graphical view ]
Pfami PF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
[Graphical view ]
PIRSFi PIRSF019029. bZIP_E4BP4. 1 hit.
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes."
    Ikushima S., Inukai T., Inaba T., Nimer S.D., Cleveland J.L., Look A.T.
    Proc. Natl. Acad. Sci. U.S.A. 94:2609-2614(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DNA-BINDING.
  2. "Identification of novel endometrial factors involved in the mouse embryo implantation."
    Shen Q.-X., Wang J., Huang Z.-P.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Embryo and Uterus.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Egg and Mammary tumor.
  5. "Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism."
    Mitsui S., Yamaguchi S., Matsuo T., Ishida Y., Okamura H.
    Genes Dev. 15:995-1006(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Parathyroid hormone-induced E4BP4/NFIL3 down-regulates transcription in osteoblasts."
    Ozkurt I.C., Tetradis S.
    J. Biol. Chem. 278:26803-26809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, TISSUE SPECIFICITY.
  7. "Parathyroid hormone induces E4bp4 messenger ribonucleic acid expression primarily through cyclic adenosine 3',5'-monophosphate signaling in osteoblasts."
    Ozkurt I.C., Pirih F.Q., Tetradis S.
    Endocrinology 145:3696-3703(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DNA-BINDING.
  8. "The negative transcription factor E4BP4 is associated with circadian clock protein PERIOD2."
    Ohno T., Onishi Y., Ishida N.
    Biochem. Biophys. Res. Commun. 354:1010-1015(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PER2 AND CRY2.
  9. "A novel E4BP4 element drives circadian expression of mPeriod2."
    Ohno T., Onishi Y., Ishida N.
    Nucleic Acids Res. 35:648-655(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNFIL3_MOUSE
AccessioniPrimary (citable) accession number: O08750
Secondary accession number(s): Q3ULK2, Q497U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi