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O08750

- NFIL3_MOUSE

UniProt

O08750 - NFIL3_MOUSE

Protein

Nuclear factor interleukin-3-regulated protein

Gene

Nfil3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites By similarity. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells By similarity. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death.By similarity4 Publications

    GO - Molecular functioni

    1. protein binding Source: BHF-UCL
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: Ensembl
    3. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI

    GO - Biological processi

    1. cellular response to interleukin-4 Source: MGI
    2. circadian rhythm Source: InterPro
    3. positive regulation of gene expression Source: MGI

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear factor interleukin-3-regulated protein
    Alternative name(s):
    E4 promoter-binding protein 4
    Embryo implantation-related NFIL3/E4BP4-like transcription factor
    Gene namesi
    Name:Nfil3
    Synonyms:E4bp4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:109495. Nfil3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Nuclear factor interleukin-3-regulated proteinPRO_0000292668Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei301 – 3011PhosphoserineBy similarity
    Modified residuei353 – 3531PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO08750.

    PTM databases

    PhosphoSiteiO08750.

    Expressioni

    Tissue specificityi

    Expressed in suprachiasmatic nucleus and liver (at protein level). Expressed in suprachiasmatic nucleus, hippocampus, gyrus dentatus, piriform cortex, internal granular layer of olfactory bulb, dorsomedial hypothalamic nucleus, pontine nuclei, granular layer of cerebellum, liver and calvariae osteoblasts.2 Publications

    Inductioni

    Expression is regulated by circadian rhythms. Up-regulated by parathyroid hormone (PTH) (at protein level). Up-regulated by IL-3, forskolin, 8-bromo-cAMP, phorbol myristate acetate and PTH in primary osteoblasts and calvariae.3 Publications

    Gene expression databases

    BgeeiO08750.
    CleanExiMM_NFIL3.
    GenevestigatoriO08750.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Binds DNA as a dimer By similarity. Interacts with DR1 By similarity. Interacts with PER2 and CRY2.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO08750.
    SMRiO08750. Positions 41-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 13664bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 9517Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni99 – 1068Leucine-zipperPROSITE-ProRule annotation
    Regioni281 – 420140Necessary for transcriptional repression and sufficient for interaction with PER2Add
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. NFIL3 subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG260819.
    GeneTreeiENSGT00530000064087.
    HOGENOMiHOG000059596.
    HOVERGENiHBG105717.
    InParanoidiO08750.
    KOiK09059.
    OMAiWHQKELN.
    OrthoDBiEOG7GQXWM.
    PhylomeDBiO08750.
    TreeFamiTF328374.

    Family and domain databases

    InterProiIPR004827. bZIP.
    IPR016743. TF_bZIP_E4BP4.
    IPR010533. Vert_IL3-reg_TF.
    [Graphical view]
    PfamiPF07716. bZIP_2. 1 hit.
    PF06529. Vert_IL3-reg_TF. 1 hit.
    [Graphical view]
    PIRSFiPIRSF019029. bZIP_E4BP4. 1 hit.
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08750-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLRKMQTIK KEPAPLDPTS SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG    50
    SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV 100
    LENKLIALGE ENATLKAELL SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ 150
    DYQTSKAAVS SFVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ 200
    ESPAQGGCRS PENKFPVIKQ EPVELESFAR EAREERGTYS TSIYQSYMGS 250
    SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH 300
    SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK 350
    LSSPADAIAK RHFDLEKHGT SGMAHSSLPP FSVQVTNIQD WSLKSEHWHH 400
    KELSSKTQSS FKTGVVEVKD GGYKVSEAEN LYLKQGIANL SAEVVSLKRF 450
    IATQPISASD SR 462
    Length:462
    Mass (Da):50,943
    Last modified:July 1, 1997 - v1
    Checksum:iDC711EB4CAC0112E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011L → F in BAE26446. (PubMed:16141072)Curated
    Sequence conflicti141 – 1411L → F in AAI00385. (PubMed:15489334)Curated
    Sequence conflicti189 – 1891D → G in AAI00385. (PubMed:15489334)Curated
    Sequence conflicti450 – 4501F → V in BAE26446. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83148 Genomic DNA. Translation: AAB53973.1.
    AY061760 mRNA. Translation: AAL29942.1.
    AK145451 mRNA. Translation: BAE26446.1.
    BC031377 mRNA. Translation: AAH31377.1.
    BC100384 mRNA. Translation: AAI00385.1.
    CCDSiCCDS26519.1.
    RefSeqiNP_059069.1. NM_017373.3.
    XP_006516938.1. XM_006516875.1.
    XP_006516939.1. XM_006516876.1.
    XP_006516940.1. XM_006516877.1.
    XP_006516941.1. XM_006516878.1.
    XP_006516942.1. XM_006516879.1.
    UniGeneiMm.136604.

    Genome annotation databases

    EnsembliENSMUST00000071065; ENSMUSP00000065363; ENSMUSG00000056749.
    GeneIDi18030.
    KEGGimmu:18030.
    UCSCiuc007qnh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83148 Genomic DNA. Translation: AAB53973.1 .
    AY061760 mRNA. Translation: AAL29942.1 .
    AK145451 mRNA. Translation: BAE26446.1 .
    BC031377 mRNA. Translation: AAH31377.1 .
    BC100384 mRNA. Translation: AAI00385.1 .
    CCDSi CCDS26519.1.
    RefSeqi NP_059069.1. NM_017373.3.
    XP_006516938.1. XM_006516875.1.
    XP_006516939.1. XM_006516876.1.
    XP_006516940.1. XM_006516877.1.
    XP_006516941.1. XM_006516878.1.
    XP_006516942.1. XM_006516879.1.
    UniGenei Mm.136604.

    3D structure databases

    ProteinModelPortali O08750.
    SMRi O08750. Positions 41-124.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O08750.

    Proteomic databases

    PRIDEi O08750.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000071065 ; ENSMUSP00000065363 ; ENSMUSG00000056749 .
    GeneIDi 18030.
    KEGGi mmu:18030.
    UCSCi uc007qnh.1. mouse.

    Organism-specific databases

    CTDi 4783.
    MGIi MGI:109495. Nfil3.

    Phylogenomic databases

    eggNOGi NOG260819.
    GeneTreei ENSGT00530000064087.
    HOGENOMi HOG000059596.
    HOVERGENi HBG105717.
    InParanoidi O08750.
    KOi K09059.
    OMAi WHQKELN.
    OrthoDBi EOG7GQXWM.
    PhylomeDBi O08750.
    TreeFami TF328374.

    Miscellaneous databases

    NextBioi 293109.
    PROi O08750.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08750.
    CleanExi MM_NFIL3.
    Genevestigatori O08750.

    Family and domain databases

    InterProi IPR004827. bZIP.
    IPR016743. TF_bZIP_E4BP4.
    IPR010533. Vert_IL3-reg_TF.
    [Graphical view ]
    Pfami PF07716. bZIP_2. 1 hit.
    PF06529. Vert_IL3-reg_TF. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF019029. bZIP_E4BP4. 1 hit.
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes."
      Ikushima S., Inukai T., Inaba T., Nimer S.D., Cleveland J.L., Look A.T.
      Proc. Natl. Acad. Sci. U.S.A. 94:2609-2614(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, DNA-BINDING.
    2. "Identification of novel endometrial factors involved in the mouse embryo implantation."
      Shen Q.-X., Wang J., Huang Z.-P.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Embryo and Uterus.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and FVB/N.
      Tissue: Egg and Mammary tumor.
    5. "Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism."
      Mitsui S., Yamaguchi S., Matsuo T., Ishida Y., Okamura H.
      Genes Dev. 15:995-1006(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Parathyroid hormone-induced E4BP4/NFIL3 down-regulates transcription in osteoblasts."
      Ozkurt I.C., Tetradis S.
      J. Biol. Chem. 278:26803-26809(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, TISSUE SPECIFICITY.
    7. "Parathyroid hormone induces E4bp4 messenger ribonucleic acid expression primarily through cyclic adenosine 3',5'-monophosphate signaling in osteoblasts."
      Ozkurt I.C., Pirih F.Q., Tetradis S.
      Endocrinology 145:3696-3703(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, DNA-BINDING.
    8. "The negative transcription factor E4BP4 is associated with circadian clock protein PERIOD2."
      Ohno T., Onishi Y., Ishida N.
      Biochem. Biophys. Res. Commun. 354:1010-1015(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PER2 AND CRY2.
    9. "A novel E4BP4 element drives circadian expression of mPeriod2."
      Ohno T., Onishi Y., Ishida N.
      Nucleic Acids Res. 35:648-655(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiNFIL3_MOUSE
    AccessioniPrimary (citable) accession number: O08750
    Secondary accession number(s): Q3ULK2, Q497U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3