O08738 (CASP6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase-6
Short name=CASP-6
EC=3.4.22.59

Alternative name(s):
Apoptotic protease Mch-2

Cleaved into the following 2 chains:

1. Caspase-6 subunit p18
2. Caspase-6 subunit p11

Gene names

Name:

Casp6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death By similarity.
Catalytic activity	Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.
Enzyme regulation	Activation is suppressed by phosphorylation at Ser-239 By similarity.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit By similarity. Interacts with BIRC6/bruce By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Highly expressed in lung, liver, kidney, testis, and heart. Lower levels in spleen, skeletal muscle and brain.
Post-translational modification	Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits By similarity.
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 5	5	By similarity		PRO_0000004612
	Chain	6 – 162	157	Caspase-6 subunit p18 By similarity		PRO_0000004613
	Propeptide	163 – 176	14	By similarity		PRO_0000004614
	Chain	177 – 276	100	Caspase-6 subunit p11 By similarity		PRO_0000004615
Sites
	Active site	104	1	By similarity
	Active site	146	1	By similarity
Amino acid modifications
	Modified residue	239	1	Phosphoserine; by NUAK1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08738 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5965DE9321126B6C	FASTA	276	31,595
	10 20 30 40 50 60 MTETDGFYKS REVFDPAEQY KMDHKRRGVA LIFNHERFFW HLTLPERRGT NADRDNLTRR 70 80 90 100 110 120 FSDLGFEVKC FNDLRAEELL LKIHEVSTSS HIDADCFICV FLSHGEGNHV YAYDAKIEIQ 130 140 150 160 170 180 TLTGLFKGDK CQSLVGKPKI FIIQACRGSQ HDVPVVPLDM VDHQTDKLDN VTQVDAASVY 190 200 210 220 230 240 TLPAGADFLM CYSVAEGYYS HRETVNGSWY IQDLCEMLAR YGSSLEFTEL LTLVNRKVSQ 250 260 270 RRVDFCKDPD AIGKKQVPCF ASMLTKKLHF CPKPSK

References

[1]	"Characterization of seven murine caspase family members." van de Craen M., Vandenabeele P., Declercq W., van den Brande I., van Loo G., Molemans F., Schotte P., van Criekinge W., Beyaert R., Fiers W. FEBS Lett. 403:61-69(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/An.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13087 mRNA. Translation: CAA73529.1.
IPI	IPI00115542.
RefSeq	NP_033941.3. NM_009811.3.
UniGene	Mm.281379.
3D structure databases
ProteinModelPortal	O08738.
SMR	O08738. Positions 14-273.
ModBase	Search...
Protein family/group databases
MEROPS	C14.005.
PTM databases
PhosphoSite	O08738.
Proteomic databases
PaxDb	O08738.
PRIDE	O08738.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029626; ENSMUSP00000029626; ENSMUSG00000027997.
GeneID	12368.
KEGG	mmu:12368.
Organism-specific databases
CTD	839.
MGI	MGI:1312921. Casp6.
Phylogenomic databases
eggNOG	NOG278720.
HOGENOM	HOG000231878.
HOVERGEN	HBG050802.
InParanoid	O08738.
KO	K04396.
OMA	DFCKDPS.
OrthoDB	EOG4MKNGQ.
Enzyme and pathway databases
BRENDA	3.4.22.59. 3474.
Gene expression databases
ArrayExpress	O08738.
Bgee	O08738.
CleanEx	MM_CASP6.
Genevestigator	O08738.
GermOnline	ENSMUSG00000027997. Mus musculus.
Family and domain databases
InterPro	IPR015469. Caspase_6. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454. PTHR10454. 1 hit. PTHR10454:SF29. PTHR10454:SF29. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CASP6. mouse.
NextBio	281056.
SOURCE	Search...

Entry information

Entry name

CASP6_MOUSE

Accession

Primary (citable) accession number: O08738

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program