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Protein

Bcl-2 homologous antagonist/killer

Gene

Bak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi158 – 1581Zinc; shared with dimeric partnerBy similarity
Metal bindingi162 – 1621Zinc; shared with dimeric partnerBy similarity

GO - Molecular functioni

GO - Biological processi

  • activation of cysteine-type endopeptidase activity Source: MGI
  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: MGI
  • aging Source: Ensembl
  • apoptotic process Source: MGI
  • apoptotic process involved in patterning of blood vessels Source: MGI
  • apoptotic signaling pathway Source: MGI
  • B cell apoptotic process Source: MGI
  • B cell homeostasis Source: MGI
  • B cell negative selection Source: MGI
  • blood vessel remodeling Source: MGI
  • brain development Source: Ensembl
  • cell proliferation Source: MGI
  • cellular response to mechanical stimulus Source: Ensembl
  • cellular response to UV Source: UniProtKB
  • cytolysis Source: MGI
  • endocrine pancreas development Source: Ensembl
  • establishment or maintenance of transmembrane electrochemical gradient Source: HGNC
  • extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • fibroblast apoptotic process Source: MGI
  • homeostasis of number of cells Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  • leukocyte homeostasis Source: MGI
  • limb morphogenesis Source: MGI
  • mitochondrial fusion Source: MGI
  • myeloid cell homeostasis Source: MGI
  • negative regulation of cell proliferation Source: Ensembl
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: MGI
  • negative regulation of gene expression Source: Ensembl
  • negative regulation of peptidyl-serine phosphorylation Source: MGI
  • organ regeneration Source: Ensembl
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of calcium ion transport into cytosol Source: MGI
  • positive regulation of endoplasmic reticulum unfolded protein response Source: MGI
  • positive regulation of proteolysis Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: Ensembl
  • post-embryonic camera-type eye morphogenesis Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of mitochondrial membrane permeability Source: HGNC
  • regulation of mitochondrial membrane potential Source: HGNC
  • regulation of protein heterodimerization activity Source: MGI
  • regulation of protein homodimerization activity Source: MGI
  • release of cytochrome c from mitochondria Source: BHF-UCL
  • response to drug Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to fungus Source: MGI
  • response to gamma radiation Source: MGI
  • response to hydrogen peroxide Source: Ensembl
  • response to mycotoxin Source: MGI
  • response to organic cyclic compound Source: Ensembl
  • response to UV-C Source: Ensembl
  • thymocyte apoptotic process Source: MGI
  • vagina development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-111452. Activation and oligomerization of BAK protein.

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2 homologous antagonist/killer
Alternative name(s):
Apoptosis regulator BAK
Gene namesi
Name:Bak1
Synonyms:Bak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1097161. Bak1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei186 – 20318HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
  • integral component of mitochondrial outer membrane Source: BHF-UCL
  • intracellular Source: MGI
  • mitochondrial membrane Source: MGI
  • mitochondrion Source: HGNC
  • pore complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 209208Bcl-2 homologous antagonist/killerPRO_0000143060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO08734.
MaxQBiO08734.
PaxDbiO08734.
PeptideAtlasiO08734.
PRIDEiO08734.
TopDownProteomicsiE9QM21.

PTM databases

iPTMnetiO08734.
PhosphoSiteiO08734.
SwissPalmiO08734.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000057789.
CleanExiMM_BAK1.
ExpressionAtlasiO08734. baseline and differential.
GenevisibleiO08734. MM.

Interactioni

Subunit structurei

Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L) (By similarity). Interacts with BCL2A1 (PubMed:18462686). Interacts with BOP. Interacts with VDAC1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ERN1O754602EBI-822441,EBI-371750From a different organism.
Vdac2Q609303EBI-822441,EBI-444578

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-29807N.
IntActiO08734. 5 interactions.
MINTiMINT-1542426.
STRINGi10090.ENSMUSP00000077757.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi68 – 8619Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VOHX-ray1.90B65-90[»]
5FMJX-ray2.43B61-94[»]
ProteinModelPortaliO08734.
SMRiO08734. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08734.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi72 – 8615BH3Add
BLAST
Motifi115 – 13420BH1Add
BLAST
Motifi167 – 18216BH2Add
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4728. Eukaryota.
ENOG41123S0. LUCA.
GeneTreeiENSGT00530000062935.
HOVERGENiHBG002674.
InParanoidiO08734.
KOiK14021.
OMAiFQAMLQH.
OrthoDBiEOG091G0OCU.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGQGPGPP KVGCDESPSP SEQQVAQDTE EVFRSYVFYL HQQEQETQGA
60 70 80 90 100
AAPANPEMDN LPLEPNSILG QVGRQLALIG DDINRRYDTE FQNLLEQLQP
110 120 130 140 150
TAGNAYELFT KIASSLFKSG ISWGRVVALL GFGYRLALYV YQRGLTGFLG
160 170 180 190 200
QVTCFLADII LHHYIARWIA QRGGWVAALN FRRDPILTVM VIFGVVLLGQ

FVVHRFFRS
Length:209
Mass (Da):23,295
Last modified:April 1, 2015 - v3
Checksum:i49972D3CA21F4218
GO

Sequence cautioni

The sequence CAA73684 differs from that shown. Reason: Frameshift at position 50.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 523AAA → RP in CAA73684 (PubMed:9299236).Curated
Sequence conflicti134 – 1341Y → H in BAC40796 (PubMed:16141072).Curated
Sequence conflicti181 – 1811F → L in CAA73684 (PubMed:9299236).Curated
Sequence conflicti181 – 1811F → L in BAC40796 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13231 mRNA. Translation: CAA73684.1. Frameshift.
AK089220 mRNA. Translation: BAC40796.1.
AC132404 Genomic DNA. No translation available.
CCDSiCCDS28558.1.
RefSeqiNP_031549.2. NM_007523.2.
UniGeneiMm.2443.

Genome annotation databases

EnsembliENSMUST00000078691; ENSMUSP00000077757; ENSMUSG00000057789.
GeneIDi12018.
KEGGimmu:12018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13231 mRNA. Translation: CAA73684.1. Frameshift.
AK089220 mRNA. Translation: BAC40796.1.
AC132404 Genomic DNA. No translation available.
CCDSiCCDS28558.1.
RefSeqiNP_031549.2. NM_007523.2.
UniGeneiMm.2443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VOHX-ray1.90B65-90[»]
5FMJX-ray2.43B61-94[»]
ProteinModelPortaliO08734.
SMRiO08734. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29807N.
IntActiO08734. 5 interactions.
MINTiMINT-1542426.
STRINGi10090.ENSMUSP00000077757.

PTM databases

iPTMnetiO08734.
PhosphoSiteiO08734.
SwissPalmiO08734.

Proteomic databases

EPDiO08734.
MaxQBiO08734.
PaxDbiO08734.
PeptideAtlasiO08734.
PRIDEiO08734.
TopDownProteomicsiE9QM21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078691; ENSMUSP00000077757; ENSMUSG00000057789.
GeneIDi12018.
KEGGimmu:12018.

Organism-specific databases

CTDi578.
MGIiMGI:1097161. Bak1.

Phylogenomic databases

eggNOGiKOG4728. Eukaryota.
ENOG41123S0. LUCA.
GeneTreeiENSGT00530000062935.
HOVERGENiHBG002674.
InParanoidiO08734.
KOiK14021.
OMAiFQAMLQH.
OrthoDBiEOG091G0OCU.

Enzyme and pathway databases

ReactomeiR-MMU-111452. Activation and oligomerization of BAK protein.

Miscellaneous databases

EvolutionaryTraceiO08734.
PROiO08734.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057789.
CleanExiMM_BAK1.
ExpressionAtlasiO08734. baseline and differential.
GenevisibleiO08734. MM.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAK_MOUSE
AccessioniPrimary (citable) accession number: O08734
Secondary accession number(s): E9QM21, Q8C264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 1, 2015
Last modified: September 7, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.