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O08730

- GLYG_RAT

UniProt

O08730 - GLYG_RAT

Protein

Glycogenin-1

Gene

Gyg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.By similarity

    Catalytic activityi

    UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

    Cofactori

    Divalent metal ions. Required for self-glucosylation. Manganese is the most effective By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861By similarity
    Metal bindingi102 – 1021ManganeseBy similarity
    Metal bindingi104 – 1041ManganeseBy similarity
    Sitei160 – 1601Important for catalytic activityBy similarity
    Sitei163 – 1631Important for catalytic activityBy similarity
    Metal bindingi212 – 2121ManganeseBy similarity

    GO - Molecular functioni

    1. glucose binding Source: RGD
    2. glycogenin glucosyltransferase activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glycogen biosynthetic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_207828. Myoclonic epilepsy of Lafora.
    REACT_214969. Glycogen synthesis.
    REACT_227999. Glycogen breakdown (glycogenolysis).
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiGT8. Glycosyltransferase Family 8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogenin-1 (EC:2.4.1.186)
    Short name:
    GN-1
    Short name:
    GN1
    Gene namesi
    Name:Gyg1
    Synonyms:Gyg
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621785. Gyg1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 333332Glycogenin-1PRO_0000215179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Modified residuei44 – 441PhosphoserineBy similarity
    Glycosylationi195 – 1951O-linked (Glc...)By similarity

    Post-translational modificationi

    Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195.By similarity
    Phosphorylated.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiO08730.
    PRIDEiO08730.

    Expressioni

    Gene expression databases

    ArrayExpressiO08730.
    GenevestigatoriO08730.

    Interactioni

    Subunit structurei

    Homodimer tightly complexed to the 86 kDa catalytic subunit of glycogen synthase GYS1.By similarity

    Protein-protein interaction databases

    IntActiO08730. 1 interaction.
    STRINGi10116.ENSRNOP00000014837.

    Structurei

    3D structure databases

    ProteinModelPortaliO08730.
    SMRiO08730. Positions 1-267.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni284 – 31633Interaction with GYS1By similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5597.
    HOGENOMiHOG000008282.
    HOVERGENiHBG000681.
    InParanoidiO08730.
    KOiK00750.
    PhylomeDBiO08730.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF01501. Glyco_transf_8. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08730-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV    50
    LETVFDEVIM VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF 100
    MDADTLVLSN IDDLFEREEL SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL 150
    HLASEQGSFD GGDQGLLNTY FSGWATTDIT KHLPFVYNLS SLSIYSYLPA 200
    FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV SHPEFLNLWW 250
    DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS 300
    SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ 333
    Length:333
    Mass (Da):37,378
    Last modified:January 23, 2007 - v4
    Checksum:iE0B29DCDB4A82DD2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF021343 mRNA. Translation: AAB81219.1.
    U96130 mRNA. Translation: AAB53334.1.
    BC070944 mRNA. Translation: AAH70944.1.
    RefSeqiNP_112305.1. NM_031043.2.
    UniGeneiRn.3661.

    Genome annotation databases

    GeneIDi81675.
    KEGGirno:81675.
    UCSCiRGD:621785. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF021343 mRNA. Translation: AAB81219.1 .
    U96130 mRNA. Translation: AAB53334.1 .
    BC070944 mRNA. Translation: AAH70944.1 .
    RefSeqi NP_112305.1. NM_031043.2.
    UniGenei Rn.3661.

    3D structure databases

    ProteinModelPortali O08730.
    SMRi O08730. Positions 1-267.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O08730. 1 interaction.
    STRINGi 10116.ENSRNOP00000014837.

    Protein family/group databases

    CAZyi GT8. Glycosyltransferase Family 8.

    Proteomic databases

    PaxDbi O08730.
    PRIDEi O08730.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81675.
    KEGGi rno:81675.
    UCSCi RGD:621785. rat.

    Organism-specific databases

    CTDi 2992.
    RGDi 621785. Gyg1.

    Phylogenomic databases

    eggNOGi COG5597.
    HOGENOMi HOG000008282.
    HOVERGENi HBG000681.
    InParanoidi O08730.
    KOi K00750.
    PhylomeDBi O08730.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    Reactomei REACT_207828. Myoclonic epilepsy of Lafora.
    REACT_214969. Glycogen synthesis.
    REACT_227999. Glycogen breakdown (glycogenolysis).

    Miscellaneous databases

    NextBioi 615276.
    PROi O08730.

    Gene expression databases

    ArrayExpressi O08730.
    Genevestigatori O08730.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF01501. Glyco_transf_8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and developmental expression of rat glycogenin in cardiac tissue."
      Pak B.J., Sangaralingham S.J., Pang S.C.
      Mol. Cell. Biochem. 194:117-123(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Heart.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiGLYG_RAT
    AccessioniPrimary (citable) accession number: O08730
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3