O08730 (GLYG_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogenin-1
Short name=GN-1
Short name=GN1
EC=2.4.1.186

Gene names

Name:	Gyg1
Synonyms:	Gyg

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase By similarity.
Catalytic activity	UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.
Cofactor	Divalent metal ions. Required for self-glucosylation. Manganese is the most effective By similarity.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Subunit structure	Homodimer tightly complexed to the 86 kDa catalytic subunit of glycogen synthase GYS1 By similarity.
Post-translational modification	Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195 By similarity. Phosphorylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from direct assay PubMed 10710493PubMed 8224611. Source: RGD
Molecular_function	glucose binding Inferred from direct assay PubMed 10710493. Source: RGD glycogenin glucosyltransferase activity Inferred from direct assay PubMed 10710493PubMed 8224611. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 333	332	Glycogenin-1		PRO_0000215179
Regions
	Region	284 – 316	33	Interaction with GYS1 By similarity
Sites
	Active site	86	1	By similarity
	Metal binding	102	1	Manganese By similarity
	Metal binding	104	1	Manganese By similarity
	Metal binding	212	1	Manganese By similarity
	Site	160	1	Important for catalytic activity By similarity
	Site	163	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	44	1	Phosphoserine By similarity
	Glycosylation	195	1	O-linked (Glc...) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08730 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: E0B29DCDB4A82DD2	FASTA	333	37,378
	10 20 30 40 50 60 MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV LETVFDEVIM 70 80 90 100 110 120 VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF MDADTLVLSN IDDLFEREEL 130 140 150 160 170 180 SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL HLASEQGSFD GGDQGLLNTY FSGWATTDIT 190 200 210 220 230 240 KHLPFVYNLS SLSIYSYLPA FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV 250 260 270 280 290 300 SHPEFLNLWW DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS 310 320 330 SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and developmental expression of rat glycogenin in cardiac tissue." Pak B.J., Sangaralingham S.J., Pang S.C. Mol. Cell. Biochem. 194:117-123(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Heart.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF021343 mRNA. Translation: AAB81219.1. U96130 mRNA. Translation: AAB53334.1. BC070944 mRNA. Translation: AAH70944.1.
IPI	IPI00230902.
RefSeq	NP_112305.1. NM_031043.2.
UniGene	Rn.3661.
3D structure databases
ProteinModelPortal	O08730.
SMR	O08730. Positions 1-267.
ModBase	Search...
Protein-protein interaction databases
IntAct	O08730. 1 interaction.
STRING	10116.ENSRNOP00000014837.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Proteomic databases
PaxDb	O08730.
PRIDE	O08730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81675.
KEGG	rno:81675.
UCSC	RGD:621785. rat.
Organism-specific databases
CTD	2992.
RGD	621785. Gyg1.
Phylogenomic databases
eggNOG	COG5597.
HOGENOM	HOG000008282.
HOVERGEN	HBG000681.
InParanoid	O08730.
KO	K00750.
OrthoDB	EOG4PC9S0.
Enzyme and pathway databases
UniPathway	UPA00164.
Gene expression databases
Genevestigator	O08730.
GermOnline	ENSRNOG00000011146. Rattus norvegicus.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615276.

Entry information

Entry name

GLYG_RAT

Accession

Primary (citable) accession number: O08730

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 93 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program