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O08730

- GLYG_RAT

UniProt

O08730 - GLYG_RAT

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Protein

Glycogenin-1

Gene

Gyg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.By similarity

Catalytic activityi

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

Cofactori

Divalent metal ions. Required for self-glucosylation. Manganese is the most effective (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861By similarity
Metal bindingi102 – 1021ManganeseBy similarity
Metal bindingi104 – 1041ManganeseBy similarity
Sitei160 – 1601Important for catalytic activityBy similarity
Sitei163 – 1631Important for catalytic activityBy similarity
Metal bindingi212 – 2121ManganeseBy similarity

GO - Molecular functioni

  1. glucose binding Source: RGD
  2. glycogenin glucosyltransferase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glycogen biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glycogen biosynthesis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_207828. Myoclonic epilepsy of Lafora.
REACT_214969. Glycogen synthesis.
REACT_227999. Glycogen breakdown (glycogenolysis).
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogenin-1 (EC:2.4.1.186)
Short name:
GN-1
Short name:
GN1
Gene namesi
Name:Gyg1
Synonyms:Gyg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621785. Gyg1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 333332Glycogenin-1PRO_0000215179Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Glycosylationi195 – 1951O-linked (Glc...)By similarity

Post-translational modificationi

Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195.By similarity
Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO08730.
PRIDEiO08730.

Expressioni

Gene expression databases

ExpressionAtlasiO08730. baseline and differential.
GenevestigatoriO08730.

Interactioni

Subunit structurei

Homodimer tightly complexed to the 86 kDa catalytic subunit of glycogen synthase GYS1.By similarity

Protein-protein interaction databases

IntActiO08730. 1 interaction.
STRINGi10116.ENSRNOP00000014837.

Structurei

3D structure databases

ProteinModelPortaliO08730.
SMRiO08730. Positions 1-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni284 – 31633Interaction with GYS1By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5597.
HOGENOMiHOG000008282.
HOVERGENiHBG000681.
InParanoidiO08730.
KOiK00750.
PhylomeDBiO08730.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08730-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV
60 70 80 90 100
LETVFDEVIM VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF
110 120 130 140 150
MDADTLVLSN IDDLFEREEL SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL
160 170 180 190 200
HLASEQGSFD GGDQGLLNTY FSGWATTDIT KHLPFVYNLS SLSIYSYLPA
210 220 230 240 250
FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV SHPEFLNLWW
260 270 280 290 300
DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS
310 320 330
SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ
Length:333
Mass (Da):37,378
Last modified:January 23, 2007 - v4
Checksum:iE0B29DCDB4A82DD2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF021343 mRNA. Translation: AAB81219.1.
U96130 mRNA. Translation: AAB53334.1.
BC070944 mRNA. Translation: AAH70944.1.
RefSeqiNP_112305.1. NM_031043.2.
UniGeneiRn.3661.

Genome annotation databases

GeneIDi81675.
KEGGirno:81675.
UCSCiRGD:621785. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF021343 mRNA. Translation: AAB81219.1 .
U96130 mRNA. Translation: AAB53334.1 .
BC070944 mRNA. Translation: AAH70944.1 .
RefSeqi NP_112305.1. NM_031043.2.
UniGenei Rn.3661.

3D structure databases

ProteinModelPortali O08730.
SMRi O08730. Positions 1-267.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O08730. 1 interaction.
STRINGi 10116.ENSRNOP00000014837.

Protein family/group databases

CAZyi GT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbi O08730.
PRIDEi O08730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81675.
KEGGi rno:81675.
UCSCi RGD:621785. rat.

Organism-specific databases

CTDi 2992.
RGDi 621785. Gyg1.

Phylogenomic databases

eggNOGi COG5597.
HOGENOMi HOG000008282.
HOVERGENi HBG000681.
InParanoidi O08730.
KOi K00750.
PhylomeDBi O08730.

Enzyme and pathway databases

UniPathwayi UPA00164 .
Reactomei REACT_207828. Myoclonic epilepsy of Lafora.
REACT_214969. Glycogen synthesis.
REACT_227999. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 615276.
PROi O08730.

Gene expression databases

ExpressionAtlasi O08730. baseline and differential.
Genevestigatori O08730.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF01501. Glyco_transf_8. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and developmental expression of rat glycogenin in cardiac tissue."
    Pak B.J., Sangaralingham S.J., Pang S.C.
    Mol. Cell. Biochem. 194:117-123(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiGLYG_RAT
AccessioniPrimary (citable) accession number: O08730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3