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Protein

Glycogenin-1

Gene

Gyg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.By similarity

Catalytic activityi

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

Cofactori

Mn2+By similarityNote: Divalent metal ions. Required for self-glucosylation. Manganese is the most effective.By similarity

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86By similarity1
Metal bindingi102ManganeseBy similarity1
Metal bindingi104ManganeseBy similarity1
Sitei160Important for catalytic activityBy similarity1
Sitei163Important for catalytic activityBy similarity1
Metal bindingi212ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: RGD

Keywordsi

Molecular functionTransferase
Biological processGlycogen biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogenin-1 (EC:2.4.1.186)
Short name:
GN-1
Short name:
GN1
Gene namesi
Name:Gyg1
Synonyms:Gyg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621785. Gyg1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002151792 – 333Glycogenin-1CuratedAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Glycosylationi195O-linked (Glc...) tyrosineBy similarity1

Post-translational modificationi

Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195.By similarity
Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO08730.
PRIDEiO08730.

PTM databases

iPTMnetiO08730.
PhosphoSitePlusiO08730.

Interactioni

Subunit structurei

Homodimer tightly complexed to the 86 kDa catalytic subunit of glycogen synthase GYS1 (By similarity). Interacts (via C-terminus) with GYS2; required for GYS2-mediated glycogen synthesis (By similarity).By similarity

Protein-protein interaction databases

IntActiO08730. 1 interactor.
STRINGi10116.ENSRNOP00000014837.

Structurei

3D structure databases

ProteinModelPortaliO08730.
SMRiO08730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 316Interaction with GYS1By similarityAdd BLAST33

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1950. Eukaryota.
COG5597. LUCA.
HOGENOMiHOG000008282.
HOVERGENiHBG000681.
InParanoidiO08730.
KOiK00750.
PhylomeDBiO08730.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF01501. Glyco_transf_8. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV
60 70 80 90 100
LETVFDEVIM VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF
110 120 130 140 150
MDADTLVLSN IDDLFEREEL SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL
160 170 180 190 200
HLASEQGSFD GGDQGLLNTY FSGWATTDIT KHLPFVYNLS SLSIYSYLPA
210 220 230 240 250
FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV SHPEFLNLWW
260 270 280 290 300
DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS
310 320 330
SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ
Length:333
Mass (Da):37,378
Last modified:January 23, 2007 - v4
Checksum:iE0B29DCDB4A82DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021343 mRNA. Translation: AAB81219.1.
U96130 mRNA. Translation: AAB53334.1.
BC070944 mRNA. Translation: AAH70944.1.
RefSeqiNP_112305.1. NM_031043.2.
UniGeneiRn.3661.

Genome annotation databases

GeneIDi81675.
KEGGirno:81675.
UCSCiRGD:621785. rat.

Similar proteinsi

Entry informationi

Entry nameiGLYG_RAT
AccessioniPrimary (citable) accession number: O08730
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 122 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families