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O08730 (GLYG_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogenin-1

Short name=GN-1
Short name=GN1
EC=2.4.1.186
Gene names
Name:Gyg1
Synonyms:Gyg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase By similarity.

Catalytic activity

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

Cofactor

Divalent metal ions. Required for self-glucosylation. Manganese is the most effective By similarity.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Subunit structure

Homodimer tightly complexed to the 86 kDa catalytic subunit of glycogen synthase GYS1 By similarity.

Post-translational modification

Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195 By similarity.

Phosphorylated By similarity.

Sequence similarities

Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 333332Glycogenin-1
PRO_0000215179

Regions

Region284 – 31633Interaction with GYS1 By similarity

Sites

Active site861 By similarity
Metal binding1021Manganese By similarity
Metal binding1041Manganese By similarity
Metal binding2121Manganese By similarity
Site1601Important for catalytic activity By similarity
Site1631Important for catalytic activity By similarity

Amino acid modifications

Modified residue21N-acetylthreonine By similarity
Modified residue441Phosphoserine By similarity
Glycosylation1951O-linked (Glc...) By similarity

Sequences

Sequence LengthMass (Da)Tools
O08730 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: E0B29DCDB4A82DD2

FASTA33337,378
        10         20         30         40         50         60 
MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV LETVFDEVIM 

        70         80         90        100        110        120 
VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF MDADTLVLSN IDDLFEREEL 

       130        140        150        160        170        180 
SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL HLASEQGSFD GGDQGLLNTY FSGWATTDIT 

       190        200        210        220        230        240 
KHLPFVYNLS SLSIYSYLPA FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV 

       250        260        270        280        290        300 
SHPEFLNLWW DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS 

       310        320        330 
SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and developmental expression of rat glycogenin in cardiac tissue."
Pak B.J., Sangaralingham S.J., Pang S.C.
Mol. Cell. Biochem. 194:117-123(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Heart.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF021343 mRNA. Translation: AAB81219.1.
U96130 mRNA. Translation: AAB53334.1.
BC070944 mRNA. Translation: AAH70944.1.
RefSeqNP_112305.1. NM_031043.2.
UniGeneRn.3661.

3D structure databases

ProteinModelPortalO08730.
SMRO08730. Positions 1-267.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO08730. 1 interaction.
STRING10116.ENSRNOP00000014837.

Protein family/group databases

CAZyGT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbO08730.
PRIDEO08730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81675.
KEGGrno:81675.
UCSCRGD:621785. rat.

Organism-specific databases

CTD2992.
RGD621785. Gyg1.

Phylogenomic databases

eggNOGCOG5597.
HOGENOMHOG000008282.
HOVERGENHBG000681.
InParanoidO08730.
KOK00750.
PhylomeDBO08730.

Enzyme and pathway databases

UniPathwayUPA00164.

Gene expression databases

ArrayExpressO08730.
GenevestigatorO08730.

Family and domain databases

InterProIPR002495. Glyco_trans_8.
[Graphical view]
PfamPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615276.
PROO08730.

Entry information

Entry nameGLYG_RAT
AccessionPrimary (citable) accession number: O08730
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 99 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways