UniProtKB - O08715 (AKAP1_MOUSE)
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Protein
A-kinase anchor protein 1, mitochondrial
Gene
Akap1
Organism
Mus musculus (Mouse)
Status
Functioni
Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.
GO - Molecular functioni
- protein kinase A regulatory subunit binding Source: MGI
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- regulation of protein kinase A signaling Source: InterPro
Keywordsi
Molecular function | RNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-983231. Factors involved in megakaryocyte development and platelet production. |
Names & Taxonomyi
Protein namesi | Recommended name: A-kinase anchor protein 1, mitochondrialAlternative name(s): Dual specificity A-kinase-anchoring protein 1 Short name: D-AKAP-1 Protein kinase A-anchoring protein 1 Short name: PRKA1 Spermatid A-kinase anchor protein Short name: S-AKAP |
Gene namesi | Name:Akap1 Synonyms:Akap |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104729. Akap1. |
Subcellular locationi
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | MitochondrionBy similarityAdd BLAST | 29 | |
ChainiPRO_0000016660 | 30 – 857 | A-kinase anchor protein 1, mitochondrialAdd BLAST | 828 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 55 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineCombined sources | 1 | |
Modified residuei | 103 | PhosphoserineCombined sources | 1 | |
Modified residuei | 164 | PhosphoserineBy similarity | 1 | |
Modified residuei | 401 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 487 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 527 | PhosphoserineBy similarity | 1 | |
Modified residuei | 546 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O08715. |
MaxQBi | O08715. |
PaxDbi | O08715. |
PeptideAtlasi | O08715. |
PRIDEi | O08715. |
PTM databases
iPTMneti | O08715. |
PhosphoSitePlusi | O08715. |
SwissPalmi | O08715. |
Expressioni
Tissue specificityi
Highest expression in testis, heart, liver, skeletal muscle, intestine and kidney, followed by brain and lung. No expression in spleen. Isoform 1/D-AKAP1A is expressed predominantly in testis whereas isoform 4/D-AKAP1D is expressed primarily in liver.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000018428. |
CleanExi | MM_AKAP1. |
ExpressionAtlasi | O08715. baseline and differential. |
Genevisiblei | O08715. MM. |
Interactioni
Subunit structurei
Interacts with SLC8A3.1 Publication
Binary interactionsi
GO - Molecular functioni
- protein kinase A regulatory subunit binding Source: MGI
Protein-protein interaction databases
BioGridi | 198048. 3 interactors. |
IntActi | O08715. 8 interactors. |
MINTi | O08715. |
STRINGi | 10090.ENSMUSP00000018572. |
Structurei
3D structure databases
ProteinModelPortali | O08715. |
SMRi | O08715. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 561 – 625 | KHPROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 712 – 771 | TudorPROSITE-ProRule annotationAdd BLAST | 60 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 306 – 319 | PKA-RII subunit binding domainAdd BLAST | 14 |
Domaini
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Keywords - Domaini
Transit peptide, TransmembranePhylogenomic databases
eggNOGi | KOG2279. Eukaryota. ENOG410Z0DP. LUCA. |
GeneTreei | ENSGT00390000001360. |
HOGENOMi | HOG000013170. |
HOVERGENi | HBG057436. |
InParanoidi | O08715. |
KOi | K16518. |
OMAi | WWFFSRK. |
OrthoDBi | EOG091G0SWL. |
TreeFami | TF105401. |
Family and domain databases
Gene3Di | 2.40.50.90. 2 hits. 3.30.1370.10. 1 hit. |
InterProi | View protein in InterPro IPR033104. Akap1. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR036612. KH_dom_type_1_sf. IPR035437. SNase_OB-fold_sf. IPR002999. Tudor. |
PANTHERi | PTHR22948:SF18. PTHR22948:SF18. 1 hit. |
Pfami | View protein in Pfam PF00013. KH_1. 1 hit. PF00567. TUDOR. 1 hit. |
SMARTi | View protein in SMART SM00322. KH. 1 hit. SM00333. TUDOR. 1 hit. |
SUPFAMi | SSF54791. SSF54791. 1 hit. |
PROSITEi | View protein in PROSITE PS50084. KH_TYPE_1. 1 hit. PS50304. TUDOR. 1 hit. |
s (6)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 3 (identifier: O08715-1) [UniParc]FASTAAdd to basket
Also known as: D-AKAP1C, AKAP121
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAIQLRSLFP LALPGMLALL GWWWFFSRKK DRLSSSDKQV ETLKVGPAIK
60 70 80 90 100
DRRLSEEACP GVLSVAPTVT QPPGREEQRC VDKPSTEPLA LPRTRQVRRR
110 120 130 140 150
SESSGNLPSV ADTRSQPGPC RDEIAKVELS LMGDKAKSIP LGCPLLPKDA
160 170 180 190 200
SFPYEAVERC KQESALGKTP GRGWPSPYAA SGEKARETGG TEGTGDAVLG
210 220 230 240 250
ENVSEEGLLS QECVSEVEKS EFPILAPGGG EGEEVSHGPP QVAELLKKEE
260 270 280 290 300
YIVGKLPSSF VEPVHSEPVK DEDALEPQVK GSSNTSDRDL AGELDKDETV
310 320 330 340 350
PENDQIKQAA FQLISQVILE ATEEFRATTV GKTVAQVHPT SATQPKGKEE
360 370 380 390 400
SCVPASQETS LGQDTSDPAS TRTGATASPS AEALPPKTYV SCLSSPLSGP
410 420 430 440 450
TKDQKPKNSA HHISLAPCPP PVTPQRQSLE GASNPRGDDN FVACMANNSQ
460 470 480 490 500
SVLSVSSLGQ CSDPVSTSGL EDSCTETISS SGDKAMTPPL PVSTQPFSNG
510 520 530 540 550
VLKEELSDLG TEDGWTMDTE ADHSGGSDGN SMDSVDSCCG LTKPDSPQSV
560 570 580 590 600
QAGSNPKKVD LIIWEIEVPK HLVGRLIGKQ GRYVSFLKQT SGAKIYISTL
610 620 630 640 650
PYTQNIQICH IEGSQHHVDK ALNLIGKKFK ELNLTNIYAP PLPSLALPSL
660 670 680 690 700
PMTSWLMLPD GITVEVIVVN QVNAGHLFVQ QHTHPTFHAL RSLDQQMYLC
710 720 730 740 750
YSQPGIPTLP TPVEITVICA APGADGAWWR AQVVASYEET NEVEIRYVDY
760 770 780 790 800
GGYKRVKVDV LRQIRSDFVT LPFQGAEVLL DSVVPLSDDD HFSPEADAAM
810 820 830 840 850
SEMTGNTALL AQVTSYSATG LPLIQLWSVV GDEVVLINRS LVERGLAQWV
DSYYASL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 | C → S in AAC27100 (Ref. 2) Curated | 1 | |
Sequence conflicti | 80 | C → S in AAB53740 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 80 | C → S in AAB53741 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 220 | S → I in AAC27100 (Ref. 2) Curated | 1 | |
Sequence conflicti | 325 | F → L in AAC27100 (Ref. 2) Curated | 1 | |
Sequence conflicti | 325 | F → L in AAB53740 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 325 | F → L in AAB53741 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 327 | A → P in AAB53740 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 327 | A → P in AAB53741 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 340 | T → I in AAC27100 (Ref. 2) Curated | 1 | |
Sequence conflicti | 340 | T → I in AAB53740 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 340 | T → I in AAB53741 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 486 | M → I in AAC27100 (Ref. 2) Curated | 1 | |
Sequence conflicti | 486 | M → I in AAB53740 (PubMed:9182549).Curated | 1 | |
Sequence conflicti | 486 | M → I in AAB53741 (PubMed:9182549).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002847 | 1 | M → MGCKTGPKPFGGGETIRPIR IRRCSYFTSTDSKM in isoform 2 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_002850 | 526 – 547 | GSDGN…KPDSP → VAAPPQERGHFGNGGCTGFF EC in isoform 5. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_002848 | 527 – 544 | SDGNS…GLTKP → RKVLGCFLGESGRGPIIC in isoform 1 and isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_002849 | 545 – 857 | Missing in isoform 1 and isoform 2. 1 PublicationAdd BLAST | 313 | |
Alternative sequenceiVSP_002851 | 548 – 857 | Missing in isoform 5. 1 PublicationAdd BLAST | 310 | |
Alternative sequenceiVSP_002852 | 614 – 637 | SQHHV…NLTNI → CVSVLTRRLSAPCRQSSELD WEEV in isoform 6. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_002853 | 638 – 857 | Missing in isoform 6. 1 PublicationAdd BLAST | 220 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U84389 mRNA. Translation: AAC27100.1. U95145 mRNA. Translation: AAB53740.1. U95146 mRNA. Translation: AAB53741.1. AL596180 Genomic DNA. Translation: CAI24699.1. |
CCDSi | CCDS25229.1. [O08715-1] |
RefSeqi | NP_001036006.1. NM_001042541.1. [O08715-1] NP_033778.2. NM_009648.2. [O08715-1] |
UniGenei | Mm.2969. |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | AKAP1_MOUSE | |
Accessioni | O08715Primary (citable) accession number: O08715 Secondary accession number(s): B1AR25, O08714, P97488 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 28, 2018 | |
This is version 162 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |