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UniProtKB - O08715 (AKAP1_MOUSE)

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Protein

A-kinase anchor protein 1, mitochondrial

Gene

Akap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionRNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 1, mitochondrial
Alternative name(s):
Dual specificity A-kinase-anchoring protein 1
Short name:
D-AKAP-1
Protein kinase A-anchoring protein 1
Short name:
PRKA1
Spermatid A-kinase anchor protein
Short name:
S-AKAP
Gene namesi
Name:Akap1
Synonyms:Akap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104729. Akap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionBy similarityAdd BLAST29
ChainiPRO_000001666030 – 857A-kinase anchor protein 1, mitochondrialAdd BLAST828

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei103PhosphoserineCombined sources1
Modified residuei164PhosphoserineBy similarity1
Modified residuei401PhosphothreonineBy similarity1
Modified residuei487PhosphothreonineBy similarity1
Modified residuei527PhosphoserineBy similarity1
Modified residuei546PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO08715.
MaxQBiO08715.
PaxDbiO08715.
PeptideAtlasiO08715.
PRIDEiO08715.

PTM databases

iPTMnetiO08715.
PhosphoSitePlusiO08715.
SwissPalmiO08715.

Expressioni

Tissue specificityi

Highest expression in testis, heart, liver, skeletal muscle, intestine and kidney, followed by brain and lung. No expression in spleen. Isoform 1/D-AKAP1A is expressed predominantly in testis whereas isoform 4/D-AKAP1D is expressed primarily in liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000018428.
CleanExiMM_AKAP1.
ExpressionAtlasiO08715. baseline and differential.
GenevisibleiO08715. MM.

Interactioni

Subunit structurei

Interacts with SLC8A3.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi198048. 3 interactors.
IntActiO08715. 8 interactors.
MINTiO08715.
STRINGi10090.ENSMUSP00000018572.

Structurei

3D structure databases

ProteinModelPortaliO08715.
SMRiO08715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini561 – 625KHPROSITE-ProRule annotationAdd BLAST65
Domaini712 – 771TudorPROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni306 – 319PKA-RII subunit binding domainAdd BLAST14

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Keywords - Domaini

Transit peptide, Transmembrane

Phylogenomic databases

eggNOGiKOG2279. Eukaryota.
ENOG410Z0DP. LUCA.
GeneTreeiENSGT00390000001360.
HOGENOMiHOG000013170.
HOVERGENiHBG057436.
InParanoidiO08715.
KOiK16518.
OMAiWWFFSRK.
OrthoDBiEOG091G0SWL.
TreeFamiTF105401.

Family and domain databases

Gene3Di2.40.50.90. 2 hits.
3.30.1370.10. 1 hit.
InterProiView protein in InterPro
IPR033104. Akap1.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR036612. KH_dom_type_1_sf.
IPR035437. SNase_OB-fold_sf.
IPR002999. Tudor.
PANTHERiPTHR22948:SF18. PTHR22948:SF18. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF00567. TUDOR. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00333. TUDOR. 1 hit.
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50304. TUDOR. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: O08715-1) [UniParc]FASTAAdd to basket
Also known as: D-AKAP1C, AKAP121

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIQLRSLFP LALPGMLALL GWWWFFSRKK DRLSSSDKQV ETLKVGPAIK 
        60         70         80         90        100
DRRLSEEACP GVLSVAPTVT QPPGREEQRC VDKPSTEPLA LPRTRQVRRR 
       110        120        130        140        150
SESSGNLPSV ADTRSQPGPC RDEIAKVELS LMGDKAKSIP LGCPLLPKDA 
       160        170        180        190        200
SFPYEAVERC KQESALGKTP GRGWPSPYAA SGEKARETGG TEGTGDAVLG 
       210        220        230        240        250
ENVSEEGLLS QECVSEVEKS EFPILAPGGG EGEEVSHGPP QVAELLKKEE 
       260        270        280        290        300
YIVGKLPSSF VEPVHSEPVK DEDALEPQVK GSSNTSDRDL AGELDKDETV 
       310        320        330        340        350
PENDQIKQAA FQLISQVILE ATEEFRATTV GKTVAQVHPT SATQPKGKEE 
       360        370        380        390        400
SCVPASQETS LGQDTSDPAS TRTGATASPS AEALPPKTYV SCLSSPLSGP 
       410        420        430        440        450
TKDQKPKNSA HHISLAPCPP PVTPQRQSLE GASNPRGDDN FVACMANNSQ 
       460        470        480        490        500
SVLSVSSLGQ CSDPVSTSGL EDSCTETISS SGDKAMTPPL PVSTQPFSNG 
       510        520        530        540        550
VLKEELSDLG TEDGWTMDTE ADHSGGSDGN SMDSVDSCCG LTKPDSPQSV 
       560        570        580        590        600
QAGSNPKKVD LIIWEIEVPK HLVGRLIGKQ GRYVSFLKQT SGAKIYISTL 
       610        620        630        640        650
PYTQNIQICH IEGSQHHVDK ALNLIGKKFK ELNLTNIYAP PLPSLALPSL 
       660        670        680        690        700
PMTSWLMLPD GITVEVIVVN QVNAGHLFVQ QHTHPTFHAL RSLDQQMYLC 
       710        720        730        740        750
YSQPGIPTLP TPVEITVICA APGADGAWWR AQVVASYEET NEVEIRYVDY 
       760        770        780        790        800
GGYKRVKVDV LRQIRSDFVT LPFQGAEVLL DSVVPLSDDD HFSPEADAAM 
       810        820        830        840        850
SEMTGNTALL AQVTSYSATG LPLIQLWSVV GDEVVLINRS LVERGLAQWV 

DSYYASL
Length:857
Mass (Da):92,195
Last modified:July 27, 2011 - v4
Checksum:i455679FCF95C28E6
GO
Isoform 1 (identifier: O08715-2) [UniParc]FASTAAdd to basket
Also known as: D-AKAP1A

The sequence of this isoform differs from the canonical sequence as follows:
     527-544: SDGNSMDSVDSCCGLTKP → RKVLGCFLGESGRGPIIC
     545-857: Missing.

Show »
Length:544
Mass (Da):57,680
Checksum:i24F297D5CB332F7A
GO
Isoform 2 (identifier: O08715-3) [UniParc]FASTAAdd to basket
Also known as: D-AKAP1B

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGCKTGPKPFGGGETIRPIRIRRCSYFTSTDSKM
     527-544: SDGNSMDSVDSCCGLTKP → RKVLGCFLGESGRGPIIC
     545-857: Missing.

Show »
Length:577
Mass (Da):61,311
Checksum:iF18916F0BE7B88A2
GO
Isoform 4 (identifier: O08715-4) [UniParc]FASTAAdd to basket
Also known as: D-AKAP1D

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGCKTGPKPFGGGETIRPIRIRRCSYFTSTDSKM

Show »
Length:890
Mass (Da):95,825
Checksum:iB4F8830C72620DF1
GO
Isoform 5 (identifier: O08715-5) [UniParc]FASTAAdd to basket
Also known as: S-AKAP84

The sequence of this isoform differs from the canonical sequence as follows:
     526-547: GSDGNSMDSVDSCCGLTKPDSP → VAAPPQERGHFGNGGCTGFFEC
     548-857: Missing.

Show »
Length:547
Mass (Da):58,000
Checksum:i83069FB21BFA3628
GO
Isoform 6 (identifier: O08715-6) [UniParc]FASTAAdd to basket
Also known as: AKAP100

The sequence of this isoform differs from the canonical sequence as follows:
     614-637: SQHHVDKALNLIGKKFKELNLTNI → CVSVLTRRLSAPCRQSSELDWEEV
     638-857: Missing.

Show »
Length:637
Mass (Da):68,012
Checksum:iE40401F94CFFE7DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80C → S in AAC27100 (Ref. 2) Curated1
Sequence conflicti80C → S in AAB53740 (PubMed:9182549).Curated1
Sequence conflicti80C → S in AAB53741 (PubMed:9182549).Curated1
Sequence conflicti220S → I in AAC27100 (Ref. 2) Curated1
Sequence conflicti325F → L in AAC27100 (Ref. 2) Curated1
Sequence conflicti325F → L in AAB53740 (PubMed:9182549).Curated1
Sequence conflicti325F → L in AAB53741 (PubMed:9182549).Curated1
Sequence conflicti327A → P in AAB53740 (PubMed:9182549).Curated1
Sequence conflicti327A → P in AAB53741 (PubMed:9182549).Curated1
Sequence conflicti340T → I in AAC27100 (Ref. 2) Curated1
Sequence conflicti340T → I in AAB53740 (PubMed:9182549).Curated1
Sequence conflicti340T → I in AAB53741 (PubMed:9182549).Curated1
Sequence conflicti486M → I in AAC27100 (Ref. 2) Curated1
Sequence conflicti486M → I in AAB53740 (PubMed:9182549).Curated1
Sequence conflicti486M → I in AAB53741 (PubMed:9182549).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0028471M → MGCKTGPKPFGGGETIRPIR IRRCSYFTSTDSKM in isoform 2 and isoform 4. 1 Publication1
Alternative sequenceiVSP_002850526 – 547GSDGN…KPDSP → VAAPPQERGHFGNGGCTGFF EC in isoform 5. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_002848527 – 544SDGNS…GLTKP → RKVLGCFLGESGRGPIIC in isoform 1 and isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_002849545 – 857Missing in isoform 1 and isoform 2. 1 PublicationAdd BLAST313
Alternative sequenceiVSP_002851548 – 857Missing in isoform 5. 1 PublicationAdd BLAST310
Alternative sequenceiVSP_002852614 – 637SQHHV…NLTNI → CVSVLTRRLSAPCRQSSELD WEEV in isoform 6. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_002853638 – 857Missing in isoform 6. 1 PublicationAdd BLAST220

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84389 mRNA. Translation: AAC27100.1.
U95145 mRNA. Translation: AAB53740.1.
U95146 mRNA. Translation: AAB53741.1.
AL596180 Genomic DNA. Translation: CAI24699.1.
CCDSiCCDS25229.1. [O08715-1]
RefSeqiNP_001036006.1. NM_001042541.1. [O08715-1]
NP_033778.2. NM_009648.2. [O08715-1]
UniGeneiMm.2969.

Genome annotation databases

EnsembliENSMUST00000018572; ENSMUSP00000018572; ENSMUSG00000018428. [O08715-1]
ENSMUST00000107903; ENSMUSP00000103536; ENSMUSG00000018428. [O08715-1]
ENSMUST00000107904; ENSMUSP00000103537; ENSMUSG00000018428. [O08715-4]
ENSMUST00000143720; ENSMUSP00000122295; ENSMUSG00000018428. [O08715-5]
GeneIDi11640.
KEGGimmu:11640.
UCSCiuc007kvu.2. mouse. [O08715-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAKAP1_MOUSE
AccessioniPrimary (citable) accession number: O08715
Secondary accession number(s): B1AR25, O08714, P97488
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: February 28, 2018
This is version 162 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome