Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O08712 (TR11B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tumor necrosis factor receptor superfamily member 11B
Alternative name(s):
Osteoclastogenesis inhibitory factor
Osteoprotegerin
Gene names
Name:Tnfrsf11b
Synonyms:Ocif, Opg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. Ref.3

Subunit structure

Homodimer. Interacts with TNFSF10 and TNFSF11.

Subcellular location

Secreted.

Tissue specificity

Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.

Developmental stage

Detected in embryo at high levels on day 7, whereas expression decreases at day 11 and increases from day 15 to 17. On day 15 found in developing bone primordia, brachiocephalic artery and ductus arteriosus, left main bronchus, abdominal aorta and midgut.

Induction

Up-regulated by TGF-beta and estrogens. Down-regulated by 1,25-dihdroxyvitamin D3 and parathyroid hormone.

Sequence similarities

Contains 2 death domains.

Contains 4 TNFR-Cys repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 401380Tumor necrosis factor receptor superfamily member 11B
PRO_0000034588

Regions

Repeat24 – 6239TNFR-Cys 1
Repeat65 – 10541TNFR-Cys 2
Repeat107 – 14236TNFR-Cys 3
Repeat145 – 18541TNFR-Cys 4
Domain198 – 26972Death 1
Domain283 – 36583Death 2

Sites

Site4001Involved in dimerization By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1651N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2891N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 54 Ref.4
Disulfide bond44 ↔ 62 Ref.4
Disulfide bond65 ↔ 80 Ref.4
Disulfide bond83 ↔ 97 Ref.4
Disulfide bond87 ↔ 105 Ref.4
Disulfide bond107 ↔ 118 Ref.4
Disulfide bond124 ↔ 142 Ref.4
Disulfide bond145 ↔ 160 Ref.4
Disulfide bond166 ↔ 185 Ref.4

Natural variations

Natural variant1381R → P in strain: 129/Ola and NIH Swiss. Ref.2
Natural variant1611I → R in strain: 129/Ola and NIH Swiss. Ref.2
Natural variant1651N → D in strain: 129/Ola and NIH Swiss. Ref.2
Natural variant2881S → A in strain: 129/Ola and NIH Swiss. Ref.2
Natural variant2961L → R in strain: 129/Ola and NIH Swiss. Ref.2

Secondary structure

......................... 401
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O08712 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: CAA6102D3B312470

FASTA40145,923
        10         20         30         40         50         60 
MNKWLCCALL VLLDIIEWTT QETLPPKYLH YDPETGHQLL CDKCAPGTYL KQHCTVRRKT 

        70         80         90        100        110        120 
LCVPCPDHSY TDSWHTSDEC VYCSPVCKEL QSVKQECNRT HNRVCECEEG RYLEIEFCLK 

       130        140        150        160        170        180 
HRSCPPGSGV VQAGTPERNT VCKKCPDGFF SGETSSKAPC IKHTNCSTFG LLLIQKGNAT 

       190        200        210        220        230        240 
HDNVCSGNRE ATQKCGIDVT LCEEAFFRFA VPTKIIPNWL SVLVDSLPGT KVNAESVERI 

       250        260        270        280        290        300 
KRRHSSQEQT FQLLKLWKHQ NRDQEMVKKI IQDIDLCESS VQRHLGHSNL TTEQLLALME 

       310        320        330        340        350        360 
SLPGKKISPE EIERTRKTCK SSEQLLKLLS LWRIKNGDQD TLKGLMYALK HLKTSHFPKT 

       370        380        390        400 
VTHSLRKTMR FLHSFTMYRL YQKLFLEMIG NQVQSVKISC L 

« Hide

References

[1]"Osteoprotegerin: a novel secreted protein involved in the regulation of bone density."
Simonet W.S., Lacey D.L., Dunstan C.R., Kelley M., Chang M.-S., Luethy R., Nguyen H.Q., Wooden S., Bennett L., Boone T., Shimamoto G., Derose M., Elliott R., Colombero A., Tan H.-L., Trail G., Sullivan J., Davy E. expand/collapse author list , Bucay N., Renshaw-Gegg L., Hughes T.M., Hill D., Pattison W., Campbell P., Sander S., Van G., Tarpley J., Derby P., Lee R., Suggs S., Boyle W.J.
Cell 89:309-319(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Kidney.
[2]"Structure of the mouse osteoclastogenesis inhibitory factor (OCIF) gene and its expression in embryogenesis."
Mizuno A., Murakami A., Nakagawa N., Yasuda H., Tsuda E., Morinaga T., Higashio K.
Gene 215:339-343(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS PRO-138; ARG-161; ASP-165; ALA-288 AND ARG-296.
Strain: 129/Ola and NIH Swiss.
Tissue: Fibroblast.
[3]"Osteoprotegerin reverses osteoporosis by inhibiting endosteal osteoclasts and prevents vascular calcification by blocking a process resembling osteoclastogenesis."
Min H., Morony S., Sarosi I., Dunstan C.R., Capparelli C., Scully S., Van G., Kaufman S., Kostenuik P.J., Lacey D.L., Boyle W.J., Simonet W.S.
J. Exp. Med. 192:463-474(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor."
Nelson C.A., Warren J.T., Wang M.W., Teitelbaum S.L., Fremont D.H.
Structure 20:1971-1982(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 22-197 IN COMPLEX WITH TNFSF11/RANKL, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U94331 mRNA. Translation: AAB53708.1.
AB013898 mRNA. Translation: BAA28269.1.
AB013903 Genomic DNA. Translation: BAA33388.1.
CCDSCCDS27468.1.
RefSeqNP_032790.3. NM_008764.3.
UniGeneMm.15383.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4E4DX-ray2.70R22-197[»]
ProteinModelPortalO08712.
SMRO08712. Positions 28-162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59980N.

PTM databases

PhosphoSiteO08712.

Proteomic databases

PRIDEO08712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079772; ENSMUSP00000078705; ENSMUSG00000063727.
GeneID18383.
KEGGmmu:18383.
UCSCuc007vrk.1. mouse.

Organism-specific databases

CTD4982.
MGIMGI:109587. Tnfrsf11b.

Phylogenomic databases

eggNOGNOG80702.
HOGENOMHOG000273896.
HOVERGENHBG061495.
InParanoidO08712.
KOK05148.
OMADIDLCEN.
OrthoDBEOG786H2Q.
PhylomeDBO08712.
TreeFamTF331157.

Gene expression databases

ArrayExpressO08712.
BgeeO08712.
GenevestigatorO08712.

Family and domain databases

Gene3D1.10.533.10. 1 hit.
InterProIPR011029. DEATH-like_dom.
IPR000488. Death_domain.
IPR001368. TNFR/NGFR_Cys_rich_reg.
IPR022323. TNFR_11.
IPR017371. TNFR_11B.
[Graphical view]
PfamPF00531. Death. 1 hit.
PF00020. TNFR_c6. 1 hit.
[Graphical view]
PIRSFPIRSF038065. TNFR_11B. 1 hit.
PRINTSPR01961. TNFACTORR11.
PR01975. TNFACTORR11B.
SMARTSM00005. DEATH. 1 hit.
SM00208. TNFR. 4 hits.
[Graphical view]
SUPFAMSSF47986. SSF47986. 2 hits.
PROSITEPS50017. DEATH_DOMAIN. 1 hit.
PS00652. TNFR_NGFR_1. 1 hit.
PS50050. TNFR_NGFR_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio293972.
PROO08712.
SOURCESearch...

Entry information

Entry nameTR11B_MOUSE
AccessionPrimary (citable) accession number: O08712
Secondary accession number(s): O70202
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot