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O08709

- PRDX6_MOUSE

UniProt

O08709 - PRDX6_MOUSE

Protein

Peroxiredoxin-6

Gene

Prdx6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury By similarity.By similarity

    Catalytic activityi

    2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
    2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei32 – 321For phospholipase activityBy similarity
    Active sitei47 – 471Cysteine sulfenic acid (-SOH) intermediateBy similarity

    GO - Molecular functioni

    1. glutathione peroxidase activity Source: UniProtKB-EC
    2. hydrolase activity Source: UniProtKB-KW
    3. peroxidase activity Source: MGI
    4. peroxiredoxin activity Source: UniProtKB-EC

    GO - Biological processi

    1. bleb assembly Source: MGI
    2. lipid catabolic process Source: UniProtKB-KW
    3. response to reactive oxygen species Source: MGI

    Keywords - Molecular functioni

    Antioxidant, Hydrolase, Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi1.11.1.15. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxiredoxin-6 (EC:1.11.1.15)
    Alternative name(s):
    1-Cys peroxiredoxin
    Short name:
    1-Cys PRX
    Acidic calcium-independent phospholipase A2 (EC:3.1.1.-)
    Short name:
    aiPLA2
    Antioxidant protein 2
    Non-selenium glutathione peroxidase (EC:1.11.1.9)
    Short name:
    NSGPx
    Gene namesi
    Name:Prdx6
    Synonyms:Aop2, Ltw4, Prdx5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:894320. Prdx6.

    Subcellular locationi

    Cytoplasm By similarity. Lysosome By similarity. Cytoplasmic vesicle By similarity
    Note: Also found in lung secretory organelles.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    2. cytosol Source: MGI
    3. lysosome Source: UniProtKB-SubCell
    4. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 224223Peroxiredoxin-6PRO_0000135103Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441PhosphothreonineBy similarity
    Disulfide bondi47 – 47Interchain; in linked formBy similarity
    Modified residuei63 – 631N6-acetyllysineBy similarity
    Modified residuei89 – 891Phosphotyrosine1 Publication
    Modified residuei209 – 2091N6-acetyllysine; alternateBy similarity
    Modified residuei209 – 2091N6-succinyllysine; alternate1 Publication

    Keywords - PTMi

    Acetylation, Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiO08709.
    PaxDbiO08709.
    PRIDEiO08709.

    2D gel databases

    COMPLUYEAST-2DPAGEO08709.
    REPRODUCTION-2DPAGEO08709.
    SWISS-2DPAGEO08709.
    UCD-2DPAGEO08709.

    PTM databases

    PhosphoSiteiO08709.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, kidney and liver. Moderate expression in brain and stomach. Very low levels in intestine.

    Gene expression databases

    CleanExiMM_PRDX5.
    MM_PRDX6.
    GenevestigatoriO08709.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with GSTP1; mediates PRDX6 glutathionylation and regeneration. Interacts with APEX1 and STH By similarity. May interact with FAM168B By similarity. May interact with HTR2A.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gstp1P191572EBI-444895,EBI-2309446

    Protein-protein interaction databases

    BioGridi198118. 1 interaction.
    IntActiO08709. 8 interactions.
    MINTiMINT-1869428.

    Structurei

    3D structure databases

    ProteinModelPortaliO08709.
    SMRiO08709. Positions 5-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 169165ThioredoxinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the AhpC/TSA family. Rehydrin subfamily.Curated
    Contains 1 thioredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0450.
    HOVERGENiHBG105234.
    InParanoidiO08709.
    KOiK11188.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000239. AHPC. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGGLLLGDE APNFEANTTI GRIRFHDFLG DSWGILFSHP RDFTPVCTTE    50
    LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN GETPTEKLPF 100
    PIIDDKGRDL AILLGMLDPV EKDDNNMPVT ARVVFIFGPD KKLKLSILYP 150
    ATTGRNFDEI LRVVDSLQLT GTKPVATPVD WKKGESVMVV PTLSEEEAKQ 200
    CFPKGVFTKE LPSGKKYLRY TPQP 224
    Length:224
    Mass (Da):24,871
    Last modified:January 23, 2007 - v3
    Checksum:iAECDEDD332858B8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti154 – 1541G → S in AAC67553. (PubMed:10395907)Curated
    Sequence conflicti181 – 1811W → R in AAD03716. (PubMed:10395907)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti124 – 1241D → A in strain: C57BL/6, C57BL/6J and FVB/N.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004670 mRNA. Translation: AAC53277.1.
    Y12883 mRNA. Translation: CAA73383.1.
    AF093852 mRNA. Translation: AAC63376.1.
    AF093853 Genomic DNA. Translation: AAC67553.1.
    AF093857
    , AF093854, AF093855, AF093856 Genomic DNA. Translation: AAD03716.1.
    AK030413 mRNA. Translation: BAC26952.1.
    BC013489 mRNA. Translation: AAH13489.1.
    CCDSiCCDS15415.1.
    RefSeqiNP_031479.1. NM_007453.3.
    XP_006544594.1. XM_006544531.1.
    UniGeneiMm.186185.

    Genome annotation databases

    GeneIDi102642619.
    11758.
    KEGGimmu:102642619.
    mmu:11758.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004670 mRNA. Translation: AAC53277.1 .
    Y12883 mRNA. Translation: CAA73383.1 .
    AF093852 mRNA. Translation: AAC63376.1 .
    AF093853 Genomic DNA. Translation: AAC67553.1 .
    AF093857
    , AF093854 , AF093855 , AF093856 Genomic DNA. Translation: AAD03716.1 .
    AK030413 mRNA. Translation: BAC26952.1 .
    BC013489 mRNA. Translation: AAH13489.1 .
    CCDSi CCDS15415.1.
    RefSeqi NP_031479.1. NM_007453.3.
    XP_006544594.1. XM_006544531.1.
    UniGenei Mm.186185.

    3D structure databases

    ProteinModelPortali O08709.
    SMRi O08709. Positions 5-224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198118. 1 interaction.
    IntActi O08709. 8 interactions.
    MINTi MINT-1869428.

    PTM databases

    PhosphoSitei O08709.

    2D gel databases

    COMPLUYEAST-2DPAGE O08709.
    REPRODUCTION-2DPAGE O08709.
    SWISS-2DPAGE O08709.
    UCD-2DPAGE O08709.

    Proteomic databases

    MaxQBi O08709.
    PaxDbi O08709.
    PRIDEi O08709.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102642619.
    11758.
    KEGGi mmu:102642619.
    mmu:11758.

    Organism-specific databases

    CTDi 9588.
    MGIi MGI:894320. Prdx6.

    Phylogenomic databases

    eggNOGi COG0450.
    HOVERGENi HBG105234.
    InParanoidi O08709.
    KOi K11188.

    Enzyme and pathway databases

    BRENDAi 1.11.1.15. 3474.

    Miscellaneous databases

    ChiTaRSi PRDX6. mouse.
    NextBioi 279511.
    PROi O08709.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PRDX5.
    MM_PRDX6.
    Genevestigatori O08709.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000239. AHPC. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LTW4 protein on mouse chromosome 1 is a member of a family of antioxidant proteins."
      Iakoubova O.A., Pacella L.A., Her H., Beier D.R.
      Genomics 42:474-478(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-26.
      Strain: C3H/FEJ, C57BL/6J and DBA/2J.
      Tissue: Kidney and Liver.
    2. "A novel type of glutathione peroxidase: expression and regulation during wound repair."
      Munz B., Frank S., Huebner G., Olsen E., Werner S.
      Biochem. J. 326:579-585(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Skin.
    3. "Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes."
      Lee T.-H., Yu S.-L., Kim S.-U., Kim Y.-M., Choi I., Kang S.W., Rhee S.G., Yu D.-Y.
      Gene 234:337-344(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/SvJ and C57BL/6.
      Tissue: Brain.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Pituitary.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    6. Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-22; 25-53; 98-106; 109-122; 145-155 AND 163-182, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    7. "The serotonin 5-HT2A and 5-HT2C receptors interact with specific sets of PDZ proteins."
      Becamel C., Gavarini S., Chanrion B., Alonso G., Galeotti N., Dumuis A., Bockaert J., Marin P.
      J. Biol. Chem. 279:20257-20266(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HTR2A.
    8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiPRDX6_MOUSE
    AccessioniPrimary (citable) accession number: O08709
    Secondary accession number(s): Q91WT2, Q9QWP4, Q9QWW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is the redox-active Cys-47 oxidized to Cys-SOH. Unlike 2-Cys peroxiredoxins, PRDX6 conserves only this peroxidatic cysteine. To regenerate and activate the enzyme, the oxidized form is directly reduced to cysteine by glutathione and not thioredoxin By similarity.By similarity
    Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO3H) upon oxidative stress.By similarity

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3