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Protein

Arginase-2, mitochondrial

Gene

Arg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated T cells. May suppress inflammation-related signaling in asthmatic airway epithelium. May play a role in promoting prenatal immune suppression. Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction. Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling. Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity.By similarity

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase-1 (Arg1), Arginase (Arg2), Arginase-2, mitochondrial (Arg2), Arginase
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120Manganese 1PROSITE-ProRule annotation1
Metal bindingi143Manganese 1PROSITE-ProRule annotation1
Metal bindingi143Manganese 2PROSITE-ProRule annotation1
Metal bindingi145Manganese 2PROSITE-ProRule annotation1
Metal bindingi147Manganese 1PROSITE-ProRule annotation1
Binding sitei202SubstrateBy similarity1
Metal bindingi251Manganese 1PROSITE-ProRule annotation1
Metal bindingi251Manganese 2PROSITE-ProRule annotation1
Metal bindingi253Manganese 2PROSITE-ProRule annotation1
Binding sitei265SubstrateBy similarity1
Binding sitei296SubstrateBy similarity1

GO - Molecular functioni

  • arginase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • apoptotic process Source: RGD
  • arginine catabolic process to ornithine Source: RGD
  • arginine metabolic process Source: RGD
  • cellular response to dexamethasone stimulus Source: RGD
  • cellular response to interferon-gamma Source: RGD
  • cellular response to interleukin-4 Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • lung development Source: RGD
  • maternal process involved in female pregnancy Source: RGD
  • midgut development Source: RGD
  • negative regulation of nitric-oxide synthase activity Source: RGD
  • negative regulation of striated muscle contraction Source: RGD
  • regulation of L-arginine import Source: RGD
  • regulation of nitric oxide biosynthetic process Source: RGD
  • response to amine Source: RGD
  • response to amino acid Source: RGD
  • response to axon injury Source: RGD
  • response to cadmium ion Source: RGD
  • response to drug Source: RGD
  • response to glucose Source: RGD
  • response to herbicide Source: RGD
  • response to hormone Source: RGD
  • response to hypoxia Source: RGD
  • response to mercury ion Source: RGD
  • response to nutrient Source: RGD
  • response to selenium ion Source: RGD
  • response to vitamin E Source: RGD
  • urea cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processAdaptive immunity, Arginine metabolism, Immunity, Innate immunity, Urea cycle
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.5.3.1 5301
SABIO-RKO08701
UniPathwayiUPA00158; UER00270

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase-2, mitochondrial (EC:3.5.3.1By similarity)
Alternative name(s):
Arginase II
Kidney-type arginase
Non-hepatic arginase
Type II arginase
Gene namesi
Name:Arg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2151 Arg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000000208623 – 354Arginase-2, mitochondrialAdd BLAST332

Proteomic databases

PaxDbiO08701
PRIDEiO08701

Interactioni

Subunit structurei

Homotrimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nos1P294762EBI-15573788,EBI-349460

Protein-protein interaction databases

DIPiDIP-61133N
IntActiO08701, 1 interactor
STRINGi10116.ENSRNOP00000015083

Structurei

3D structure databases

ProteinModelPortaliO08701
SMRiO08701
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 149Substrate bindingBy similarity5
Regioni156 – 158Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2965 Eukaryota
COG0010 LUCA
HOGENOMiHOG000204319
HOVERGENiHBG003030
InParanoidiO08701
KOiK01476
PhylomeDBiO08701

Family and domain databases

InterProiView protein in InterPro
IPR014033 Arginase
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
IPR020855 Ureohydrolase_Mn_BS
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PIRSFiPIRSF036979 Arginase, 1 hit
PRINTSiPR00116 ARGINASE
SUPFAMiSSF52768 SSF52768, 1 hit
TIGRFAMsiTIGR01229 rocF_arginase, 1 hit
PROSITEiView protein in PROSITE
PS01053 ARGINASE_1, 1 hit
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLRSSVSRL LHGQIPCALT RSVHSVAVVG APFSRGQKKK GVEYGPAAIR
60 70 80 90 100
EAGLLKRLSM LGCHIKDFGD LSFTNVPKDD PYNNLVVYPR SVGIANQELA
110 120 130 140 150
EVVSRAVSGG YSCVTLGGDH SLAIGTISGH ARHHPDLCVI WVDAHADINT
160 170 180 190 200
PLTTVSGNIH GQPLSFLIRE LQDKVPQLPG FSWIKPCLSP PNLVYIGLRD
210 220 230 240 250
VEPAEHFILK SFDIQYFSMR DIDRLGIQKV MEQTFDRLIG KRKRPIHLSF
260 270 280 290 300
DIDAFDPKLA PATGTPVVGG LTYREGLYIT EEIHSTGLLS ALDLVEVNPH
310 320 330 340 350
LATSEEEAKA TASLAVDVIA SSFGQTREGG HIAYDHLPTP SSPHESEKEE

CVRI
Length:354
Mass (Da):38,640
Last modified:July 1, 1997 - v1
Checksum:iBC03E6BC99B29B8C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88Y → N in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti90R → P in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti100A → S in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti116L → M in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti119D → Y in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti128S → I in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti143D → Y in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti164L → V in BAA13183 (PubMed:8898077).Curated1
Sequence conflicti168I → L in BAA13183 (PubMed:8898077).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90887 mRNA Translation: AAC22580.1
D86928 mRNA Translation: BAA13183.1
RefSeqiNP_062041.1, NM_019168.1
UniGeneiRn.11055

Genome annotation databases

GeneIDi29215
KEGGirno:29215
UCSCiRGD:2151 rat

Similar proteinsi

Entry informationi

Entry nameiARGI2_RAT
AccessioniPrimary (citable) accession number: O08701
Secondary accession number(s): P97539
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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