Reviewed, UniProtKB/Swiss-Prot O08701 (ARGI2_RAT)

Names and origin

Protein names

Recommended name:
    Arginase-2, mitochondrial
      Short name=Arginase II
    EC=3.5.3.1
Alternative name(s):
    Non-hepatic arginase
    Kidney-type arginase

Gene names

Name:

Arg2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis By similarity.
Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homotrimer By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism Urea cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	arginine catabolic process to ornithine Traceable author statement. Source: RGD negative regulation of nitric-oxide synthase activity Inferred from mutant phenotype. Source: RGD negative regulation of striated muscle contraction Inferred from mutant phenotype. Source: RGD regulation of nitric oxide biosynthetic process Inferred from expression pattern. Source: RGD urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	arginase activity Inferred from direct assay. Source: MGI manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 354	332	Arginase-2, mitochondrial		PRO_0000002086
Regions
	Region	145 – 149	5	Substrate binding By similarity
	Region	156 – 158	3	Substrate binding By similarity
Sites
	Metal binding	120	1	Manganese 1 By similarity
	Metal binding	143	1	Manganese 1 By similarity
	Metal binding	143	1	Manganese 2 By similarity
	Metal binding	145	1	Manganese 2 By similarity
	Metal binding	147	1	Manganese 1 By similarity
	Metal binding	251	1	Manganese 1 By similarity
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	253	1	Manganese 2 By similarity
	Binding site	202	1	Substrate By similarity
	Binding site	296	1	Substrate By similarity
Experimental info
	Sequence conflict	88	1	Y → N Ref.2
	Sequence conflict	90	1	R → P Ref.2
	Sequence conflict	100	1	A → S in BAA13183. Ref.2
	Sequence conflict	116	1	L → M in BAA13183. Ref.2
	Sequence conflict	119	1	D → Y in BAA13183. Ref.2
	Sequence conflict	128	1	S → I in BAA13183. Ref.2
	Sequence conflict	143	1	D → Y in BAA13183. Ref.2
	Sequence conflict	164	1	L → V in BAA13183. Ref.2
	Sequence conflict	168	1	I → L in BAA13183. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O08701-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: BC03E6BC99B29B8C	FASTA	354	38,640
	10 20 30 40 50 60 MFLRSSVSRL LHGQIPCALT RSVHSVAVVG APFSRGQKKK GVEYGPAAIR EAGLLKRLSM 70 80 90 100 110 120 LGCHIKDFGD LSFTNVPKDD PYNNLVVYPR SVGIANQELA EVVSRAVSGG YSCVTLGGDH 130 140 150 160 170 180 SLAIGTISGH ARHHPDLCVI WVDAHADINT PLTTVSGNIH GQPLSFLIRE LQDKVPQLPG 190 200 210 220 230 240 FSWIKPCLSP PNLVYIGLRD VEPAEHFILK SFDIQYFSMR DIDRLGIQKV MEQTFDRLIG 250 260 270 280 290 300 KRKRPIHLSF DIDAFDPKLA PATGTPVVGG LTYREGLYIT EEIHSTGLLS ALDLVEVNPH 310 320 330 340 350 LATSEEEAKA TASLAVDVIA SSFGQTREGG HIAYDHLPTP SSPHESEKEE CVRI

References

[1]	"Cloning and characterization of the mouse and rat type II arginase genes." Iyer R.K., Bando J.M., Jenkinson C.P., Vockley J.G., Kim P.S., Kern R.M., Cederbaum S.D., Grody W.W. Mol. Genet. Metab. 63:168-175(1998) [PubMed: 9608538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line." Gotoh T., Sonoki T., Nagasaki A., Terada K., Takiguchi M., Mori M. FEBS Lett. 395:119-122(1996) [PubMed: 8898077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-168. Strain: Wistar. Tissue: Small intestine.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90887 mRNA. Translation: AAC22580.1. D86928 mRNA. Translation: BAA13183.1.
IPI	IPI00231829.
RefSeq	NP_062041.1.
UniGene	Rn.11055
3D structure databases
HSSP	HSSP built from PDB template 1PQ3 based on UniProtKB P78540.
SMR	O08701. Positions 24-329.
ModBase	Search...
Protein-protein interaction databases
STRING	O08701.
Genome annotation databases
Ensembl	ENSRNOT00000015083; ENSRNOP00000015083; ENSRNOG00000011139; Rattus norvegicus. [Genome view]
GeneID	29215.
KEGG	rno:29215.
UCSC	NM_019168. rat.
Organism-specific databases
CTD	29215.
RGD	2151. Arg2.
Phylogenomic databases
HOVERGEN	O08701.
Enzyme and pathway databases
BRENDA	3.5.3.1. 248.
Gene expression databases
ArrayExpress	O08701.
Genevestigator	O08701.
GermOnline	ENSRNOG00000011139. Rattus norvegicus.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608397.

Entry information

Entry name

ARGI2_RAT

Accession

Primary (citable) accession number: O08701
Secondary accession number(s): P97539

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)