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Protein

Vacuolar protein sorting-associated protein 45

Gene

Vps45

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 45
Short name:
rVps45
Gene namesi
Name:Vps45
Synonyms:Vps45a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621267. Vps45.

Subcellular locationi

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: BHF-UCL
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Vacuolar protein sorting-associated protein 45PRO_0000206314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei307 – 3071PhosphoserineBy similarity
Modified residuei441 – 4411PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08700.
PRIDEiO08700.

PTM databases

iPTMnetiO08700.

Expressioni

Tissue specificityi

Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.

Gene expression databases

GenevisibleiO08700. RN.

Interactioni

Subunit structurei

Interacts with STX6 and ZFYVE20.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx16Q8BVI56EBI-7849203,EBI-3871057From a different organism.

Protein-protein interaction databases

BioGridi249101. 2 interactions.
IntActiO08700. 2 interactions.
MINTiMINT-4576687.
STRINGi10116.ENSRNOP00000028751.

Structurei

3D structure databases

ProteinModelPortaliO08700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1299. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00550000075028.
HOGENOMiHOG000200803.
HOVERGENiHBG059810.
InParanoidiO08700.
KOiK12479.
OMAiQFKHTKS.
OrthoDBiEOG7H791T.
PhylomeDBiO08700.
TreeFamiTF300407.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequencei

Sequence statusi: Complete.

O08700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY
60 70 80 90 100
LFERIDSQNR EIMKHLKAIC FLRPTKENVD SLIQELRRPK YSIYFIYFSN
110 120 130 140 150
VISKSDVKSL AEADEQEVVA EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW
160 170 180 190 200
DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS SEAAKRLGEC VKQVISKEYE
210 220 230 240 250
LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL GINNNRIDLS
260 270 280 290 300
RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKRPK
310 320 330 340 350
EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV
360 370 380 390 400
SEVEQELACQ NDHSSALQNV KRLLQNPKVT EFDAVRLVML YALHYERHSS
410 420 430 440 450
NSLPGLIVDL RSKGVAEKYR KLVSAVVEYG GKRVRGSDLF SPKDAVAITK
460 470 480 490 500
QFLKGLKGVE NVYTQHQPFL HETLDHLIKG KLKENLYPYL GPSTLRDRPQ
510 520 530 540 550
DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTIHNT KSFLEEVLAS
560 570
GLHSRSRESS QATSRSASRR
Length:570
Mass (Da):64,894
Last modified:July 1, 1997 - v1
Checksum:i0FF5E2D8E7E7E571
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81160 mRNA. Translation: AAB53041.1.
BC081705 mRNA. Translation: AAH81705.1.
RefSeqiNP_742069.1. NM_172072.2.
UniGeneiRn.9316.

Genome annotation databases

EnsembliENSRNOT00000028751; ENSRNOP00000028751; ENSRNOG00000021173.
GeneIDi64516.
KEGGirno:64516.
UCSCiRGD:621267. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81160 mRNA. Translation: AAB53041.1.
BC081705 mRNA. Translation: AAH81705.1.
RefSeqiNP_742069.1. NM_172072.2.
UniGeneiRn.9316.

3D structure databases

ProteinModelPortaliO08700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249101. 2 interactions.
IntActiO08700. 2 interactions.
MINTiMINT-4576687.
STRINGi10116.ENSRNOP00000028751.

PTM databases

iPTMnetiO08700.

Proteomic databases

PaxDbiO08700.
PRIDEiO08700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028751; ENSRNOP00000028751; ENSRNOG00000021173.
GeneIDi64516.
KEGGirno:64516.
UCSCiRGD:621267. rat.

Organism-specific databases

CTDi11311.
RGDi621267. Vps45.

Phylogenomic databases

eggNOGiKOG1299. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00550000075028.
HOGENOMiHOG000200803.
HOVERGENiHBG059810.
InParanoidiO08700.
KOiK12479.
OMAiQFKHTKS.
OrthoDBiEOG7H791T.
PhylomeDBiO08700.
TreeFamiTF300407.

Enzyme and pathway databases

ReactomeiR-RNO-6811438. Intra-Golgi traffic.

Miscellaneous databases

PROiO08700.

Gene expression databases

GenevisibleiO08700. RN.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45."
    El-Husseini A.E.-D., Guthrie H., Snutch T.P., Vincent S.R.
    Biochim. Biophys. Acta 1325:8-12(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPS45_RAT
AccessioniPrimary (citable) accession number: O08700
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.