O08700 (VPS45_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 45
Short name=rVps45

Gene names

Name:	Vps45
Synonyms:	Vps45a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
Subunit structure	Interacts with STX6 and ZFYVE20 By similarity.
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network By similarity.
Tissue specificity	Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane of membrane fraction Inferred from direct assay. Source: BHF-UCL
Molecular function	protein binding Inferred from physical interaction. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Vacuolar protein sorting-associated protein 45		PRO_0000206314

Sequences

	Sequence		Length	Mass (Da)
	O08700 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 0FF5E2D8E7E7E571	FASTA	570	64,894
	10 20 30 40 50 60 MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR 70 80 90 100 110 120 EIMKHLKAIC FLRPTKENVD SLIQELRRPK YSIYFIYFSN VISKSDVKSL AEADEQEVVA 130 140 150 160 170 180 EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS 190 200 210 220 230 240 SEAAKRLGEC VKQVISKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL 250 260 270 280 290 300 GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKRPK 310 320 330 340 350 360 EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ 370 380 390 400 410 420 NDHSSALQNV KRLLQNPKVT EFDAVRLVML YALHYERHSS NSLPGLIVDL RSKGVAEKYR 430 440 450 460 470 480 KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG 490 500 510 520 530 540 KLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTIHNT 550 560 570 KSFLEEVLAS GLHSRSRESS QATSRSASRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45." El-Husseini A.E.-D., Guthrie H., Snutch T.P., Vincent S.R. Biochim. Biophys. Acta 1325:8-12(1997) [PubMed: 9106478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U81160 mRNA. Translation: AAB53041.1. BC081705 mRNA. Translation: AAH81705.1.
IPI	IPI00195452.
RefSeq	NP_742069.1. NM_172072.2.
UniGene	Rn.9316.
3D structure databases
ProteinModelPortal	O08700.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4576687.
STRING	O08700.
Proteomic databases
PRIDE	O08700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028751; ENSRNOP00000028751; ENSRNOG00000021173.
GeneID	64516.
KEGG	rno:64516.
UCSC	NM_172072. rat.
Organism-specific databases
CTD	11311.
RGD	621267. Vps45.
Phylogenomic databases
eggNOG	roNOG05944.
GeneTree	ENSGT00550000075028.
HOVERGEN	HBG059810.
InParanoid	O08700.
OMA	GTTIHNT.
OrthoDB	EOG4H463H.
PhylomeDB	O08700.
Gene expression databases
ArrayExpress	O08700.
Genevestigator	O08700.
GermOnline	ENSRNOG00000021173. Rattus norvegicus.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
KO	K12479.
PANTHER	PTHR11679. Sec1-like. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
NextBio	613314.

Entry information

Entry name

VPS45_RAT

Accession

Primary (citable) accession number: O08700

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program