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Protein

15-hydroxyprostaglandin dehydrogenase [NAD(+)]

Gene

Hpgd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4 (By similarity).By similarity1 Publication

Catalytic activityi

(5Z,13E,15S)-11-alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11-alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91NAD; via carbonyl oxygenBy similarity1
Binding sitei138SubstrateBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20NADBy similarity9
Nucleotide bindingi36 – 37NADBy similarity2
Nucleotide bindingi63 – 65NADBy similarity3
Nucleotide bindingi151 – 155NADBy similarity5
Nucleotide bindingi186 – 188NADBy similarity3

GO - Molecular functioni

GO - Biological processi

  • ductus arteriosus closure Source: UniProtKB
  • female pregnancy Source: RGD
  • kidney development Source: RGD
  • negative regulation of cell cycle Source: UniProtKB
  • ovulation Source: UniProtKB
  • parturition Source: UniProtKB
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of vascular smooth muscle cell proliferation Source: RGD
  • prostaglandin metabolic process Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • response to lipopolysaccharide Source: RGD
  • thrombin-activated receptor signaling pathway Source: UniProtKB
  • transforming growth factor beta receptor signaling pathway Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid metabolism, Lipid metabolism, Prostaglandin metabolism
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.141. 5301.
ReactomeiR-RNO-2142700. Synthesis of Lipoxins (LX).

Names & Taxonomyi

Protein namesi
Recommended name:
15-hydroxyprostaglandin dehydrogenase [NAD(+)] (EC:1.1.1.141)
Short name:
15-PGDH
Alternative name(s):
Prostaglandin dehydrogenase 1
Gene namesi
Name:Hpgd
Synonyms:Pgdh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi620087. Hpgd.

Subcellular locationi

GO - Cellular componenti

  • basolateral plasma membrane Source: Ensembl
  • cytoplasm Source: RGD
  • cytosol Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • nucleoplasm Source: Ensembl

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002536271 – 26615-hydroxyprostaglandin dehydrogenase [NAD(+)]Add BLAST266

Proteomic databases

PaxDbiO08699.

PTM databases

iPTMnetiO08699.
PhosphoSitePlusiO08699.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010610.
GenevisibleiO08699. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014229.

Structurei

3D structure databases

ProteinModelPortaliO08699.
SMRiO08699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4169. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG107379.
InParanoidiO08699.
KOiK00069.
OMAiNTPILQS.
OrthoDBiEOG091G0OC9.
PhylomeDBiO08699.
TreeFamiTF324093.

Family and domain databases

InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PfamiView protein in Pfam
PF00106. adh_short. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

O08699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVNGKVALV TGAAQGIGKA FTEALLLHGA KVALVDWNLE TGVKCKAALD
60 70 80 90 100
EQFEPQKTLF IQCDVADQKQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW
110 120 130 140 150
EQTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINISSIA GLMPVAQQPV
160 170 180 190 200
YCASKHGIIG FTRSAAMAAN LMKSGVRLNV ICPGFVKTPI LESIEKEENM
210 220 230 240 250
GQYIEYTDQI KAMMKFYGIL DPSAIANGLI NLIEDDALNG AIMKITASKG
260
IHFQDYDLFP SFSKAP
Length:266
Mass (Da):28,939
Last modified:October 17, 2006 - v2
Checksum:iAF9CCD6185ABD6C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146A → T in AAB53027 (PubMed:9099857).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44750 mRNA. Translation: AAB53027.1.
BC062399 mRNA. Translation: AAH62399.1.
RefSeqiNP_077366.2. NM_024390.2.
UniGeneiRn.6386.

Genome annotation databases

EnsembliENSRNOT00000014229; ENSRNOP00000014229; ENSRNOG00000010610.
GeneIDi79242.
KEGGirno:79242.
UCSCiRGD:620087. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPGDH_RAT
AccessioniPrimary (citable) accession number: O08699
Secondary accession number(s): Q6P687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: June 7, 2017
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families