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Protein

ADP-ribosylation factor-like protein 2

Gene

Arl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca2+-dependent release of acetylcholine. Required for normal progress through the cell cycle (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 308GTPBy similarity
Nucleotide bindingi66 – 705GTPBy similarity
Nucleotide bindingi125 – 1284GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 2
Gene namesi
Name:Arl2
Synonyms:Arl184
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69326. Arl2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • Golgi apparatus Source: RGD
  • lateral plasma membrane Source: UniProtKB
  • mitochondrial intermembrane space Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 184183ADP-ribosylation factor-like protein 2PRO_0000207455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Modified residuei45 – 451PhosphoserineCombined sources
Cross-linki71 – 71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Not N-myristoylated.

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO08697.
PRIDEiO08697.

PTM databases

iPTMnetiO08697.
PhosphoSiteiO08697.

Expressioni

Tissue specificityi

Expressed in brain, retina, lung, cerebellum, liver, kidney, hippocampus, spleen, cortex and heart (at protein level).3 Publications

Interactioni

Subunit structurei

Found in a complex with ARL2, ARL2BP and SLC25A6. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with ELMOD2. The GTP-bound form interacts with ARL2BP. The GDP-bound form interacts preferentially with TBCD. Interacts with UNC119. Found in a complex with ARL2, ARL2BP and SLC25A4. The GTP-bound form interacts with PDE6D (By similarity).By similarity

Protein-protein interaction databases

BioGridi249263. 1 interaction.
STRINGi10116.ENSRNOP00000028525.

Structurei

3D structure databases

ProteinModelPortaliO08697.
SMRiO08697. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0073. Eukaryota.
ENOG410XRG9. LUCA.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiO08697.
KOiK07943.
PhylomeDBiO08697.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLTILKKM KQKERDVRLL MLGLDNAGKT TILKKFNGED VDTISPTLGF
60 70 80 90 100
NIKTLEHRGF KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ
110 120 130 140 150
DCQRELQSLL VEERLAGATL LIFANKQDLP GALSCNAIQE ALELDSIRSH
160 170 180
HWRIQGCSAV TGEDLLPGID WLLDDISSRV FTAD
Length:184
Mass (Da):20,836
Last modified:July 1, 1997 - v1
Checksum:i96993CCBECF6EE53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12708 mRNA. Translation: CAA73245.1.
RefSeqiNP_113899.1. NM_031711.1.
UniGeneiRn.36965.

Genome annotation databases

GeneIDi65142.
KEGGirno:65142.
UCSCiRGD:69326. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12708 mRNA. Translation: CAA73245.1.
RefSeqiNP_113899.1. NM_031711.1.
UniGeneiRn.36965.

3D structure databases

ProteinModelPortaliO08697.
SMRiO08697. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249263. 1 interaction.
STRINGi10116.ENSRNOP00000028525.

PTM databases

iPTMnetiO08697.
PhosphoSiteiO08697.

Proteomic databases

PaxDbiO08697.
PRIDEiO08697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65142.
KEGGirno:65142.
UCSCiRGD:69326. rat.

Organism-specific databases

CTDi402.
RGDi69326. Arl2.

Phylogenomic databases

eggNOGiKOG0073. Eukaryota.
ENOG410XRG9. LUCA.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiO08697.
KOiK07943.
PhylomeDBiO08697.

Miscellaneous databases

PROiO08697.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL2_RAT
AccessioniPrimary (citable) accession number: O08697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.