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Protein

Forkhead box protein M1

Gene

Foxm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi233 – 325Fork-headPROSITE-ProRule annotationAdd BLAST93

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • protein kinase binding Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator Source: UniProtKB
  • DNA repair Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: UniProtKB
  • liver development Source: MGI
  • negative regulation of cell aging Source: MGI
  • negative regulation of stress-activated MAPK cascade Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of double-strand break repair Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of cell cycle arrest Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of Ras protein signal transduction Source: MGI
  • regulation of reactive oxygen species metabolic process Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vasculogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein M1
Alternative name(s):
Forkhead homolog 16
Winged-helix transcription factor Trident
Gene namesi
Name:Foxm1
Synonyms:Fkh16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1347487. Foxm1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918641 – 760Forkhead box protein M1Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki199Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei329PhosphoserineBy similarity1
Cross-linki354Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei374Phosphoserine; by CHEK2By similarity1
Cross-linki420Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki438Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei521PhosphoserineBy similarity1
Modified residuei608Phosphothreonine; by CDK1By similarity1
Modified residuei624PhosphothreonineBy similarity1
Modified residuei727Phosphoserine; by PLK1By similarity1
Modified residuei736Phosphoserine; by PLK1By similarity1

Post-translational modificationi

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO08696.
PaxDbiO08696.
PRIDEiO08696.

PTM databases

iPTMnetiO08696.
PhosphoSitePlusiO08696.

Expressioni

Tissue specificityi

Expressed in fetal heart, brain, liver, lung, kidney and limb, but only in adult thymus. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors.

Developmental stagei

Expressed at E14 day in the embryo.

Gene expression databases

CleanExiMM_FOXM1.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiO08696. 2 interactors.
STRINGi10090.ENSMUSP00000073041.

Structurei

3D structure databases

ProteinModelPortaliO08696.
SMRiO08696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi479 – 512Glu/Pro/Ser/Thr-richAdd BLAST34

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
HOGENOMiHOG000112633.
HOVERGENiHBG051652.
InParanoidiO08696.
PhylomeDBiO08696.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTSPRRPLI LKRRRLPLPV QNAPSETSEE EAKRSPAQPE PAPAQASQEV
60 70 80 90 100
AESSSCKFPA GIKIINHPTT PNTQVVAIPS NADIQSIITA LTAKGKESGT
110 120 130 140 150
SGPNRFILIS SGGPSSHPSQ PQAHSSRDSK RAEVITETLG PKPAAKGVPV
160 170 180 190 200
PKPPGAPPRQ RQESYAGGEA AGCTLDNSLT NIQWLGKMSS DGLGPCSVKQ
210 220 230 240 250
ELEEKENCHL EQNRVKVEEP SGVSTSWQDS VSERPPYSYM AMIQFAINST
260 270 280 290 300
ERKRMTLKDI YTWIEDHFPY FKHIAKPGWK NSIRHNLSLH DMFVRETSAN
310 320 330 340 350
GKVSFWTIHP SANRHLTLDQ VFKPLEPGSP QSPEHLESQQ KRPNPELHRN
360 370 380 390 400
VTIKTEIPLG ARRKMKPLLP RVSSYLEPIQ FPVNQSLVLQ PSVKVPFRLA
410 420 430 440 450
ASLMSSELAR HSKRVRIAPK VLLSSEGIAP LPATEPPKEE KPLLGGEGLL
460 470 480 490 500
PLLPIQSIKE EEMQPEEDIA HLERPIKVES PPLEEWPSPC ASLKEELSNS
510 520 530 540 550
WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV SRSRRKQHLQ
560 570 580 590 600
PPCLDEPDLF FPEDSSTFRP AVELLAESSE PAPHLSCPQE EGGPFKTPIK
610 620 630 640 650
ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGLLPDS
660 670 680 690 700
LGLMELNTTP LKSGPLFDSP RELLNSEPFD LASDPFGSPP PPHVEGPKPG
710 720 730 740 750
SPELQIPSLS ANRSLTEGLV LDTMNDSLSK ILLDISFPGL EEDPLGPDNI
760
NWSQFIPELR
Length:760
Mass (Da):83,694
Last modified:December 1, 2000 - v2
Checksum:iAA614CB940EE7492
GO

Sequence cautioni

The sequence CAA72115 differs from that shown. Reason: Erroneous termination at position 758. Translated as Glu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11245 mRNA. Translation: CAA72115.1. Sequence problems.
UniGeneiMm.42148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11245 mRNA. Translation: CAA72115.1. Sequence problems.
UniGeneiMm.42148.

3D structure databases

ProteinModelPortaliO08696.
SMRiO08696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08696. 2 interactors.
STRINGi10090.ENSMUSP00000073041.

PTM databases

iPTMnetiO08696.
PhosphoSitePlusiO08696.

Proteomic databases

MaxQBiO08696.
PaxDbiO08696.
PRIDEiO08696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1347487. Foxm1.

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
HOGENOMiHOG000112633.
HOVERGENiHBG051652.
InParanoidiO08696.
PhylomeDBiO08696.

Miscellaneous databases

ChiTaRSiFoxm1. mouse.
PROiO08696.
SOURCEiSearch...

Gene expression databases

CleanExiMM_FOXM1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. Fork_head_dom.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOXM1_MOUSE
AccessioniPrimary (citable) accession number: O08696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.