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Protein

Arginase-2, mitochondrial

Gene

Arg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis.By similarity

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathway:iurea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase-2, mitochondrial (Arg2), Arginase-1 (Arg1), Arginase (Arg2)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201Manganese 1PROSITE-ProRule annotation
Metal bindingi143 – 1431Manganese 1PROSITE-ProRule annotation
Metal bindingi143 – 1431Manganese 2PROSITE-ProRule annotation
Metal bindingi145 – 1451Manganese 2PROSITE-ProRule annotation
Metal bindingi147 – 1471Manganese 1PROSITE-ProRule annotation
Binding sitei202 – 2021SubstrateBy similarity
Metal bindingi251 – 2511Manganese 1PROSITE-ProRule annotation
Metal bindingi251 – 2511Manganese 2PROSITE-ProRule annotation
Metal bindingi253 – 2531Manganese 2PROSITE-ProRule annotation
Binding sitei296 – 2961SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • arginine metabolic process Source: UniProtKB-KW
  • striated muscle contraction Source: MGI
  • urea cycle Source: UniProtKB-UniPathway
  • ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism, Urea cycle

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_315699. Urea cycle.
UniPathwayiUPA00158; UER00270.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase-2, mitochondrial (EC:3.5.3.1)
Alternative name(s):
Kidney-type arginase
Non-hepatic arginase
Type II arginase
Gene namesi
Name:Arg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1330806. Arg2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionSequence AnalysisAdd
BLAST
Chaini23 – 354332Arginase-2, mitochondrialPRO_0000002085Add
BLAST

Proteomic databases

MaxQBiO08691.
PaxDbiO08691.
PRIDEiO08691.

2D gel databases

REPRODUCTION-2DPAGEO08691.

PTM databases

PhosphoSiteiO08691.

Expressioni

Gene expression databases

BgeeiO08691.
CleanExiMM_ARG2.
GenevisibleiO08691. MM.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

IntActiO08691. 1 interaction.
MINTiMINT-4088250.
STRINGi10090.ENSMUSP00000021550.

Structurei

3D structure databases

ProteinModelPortaliO08691.
SMRiO08691. Positions 24-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni145 – 1495Substrate bindingBy similarity
Regioni156 – 1583Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0010.
GeneTreeiENSGT00530000063082.
HOGENOMiHOG000204319.
HOVERGENiHBG003030.
InParanoidiO08691.
KOiK01476.
OMAiIATCFGQ.
OrthoDBiEOG747PJ5.
PhylomeDBiO08691.
TreeFamiTF300034.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR014033. Arginase.
IPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PIRSFiPIRSF036979. Arginase. 1 hit.
PRINTSiPR00116. ARGINASE.
TIGRFAMsiTIGR01229. rocF_arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLRSSASRL LHGQIPCVLT RSVHSVAIVG APFSRGQKKL GVEYGPAAIR
60 70 80 90 100
EAGLLKRLSR LGCHLKDFGD LSFTNVPQDD PYNNLVVYPR SVGLANQELA
110 120 130 140 150
EVVSRAVSGG YSCVTMGGDH SLAIGTIIGH ARHRPDLCVI WVDAHADINT
160 170 180 190 200
PLTTVSGNIH GQPLSFLIKE LQDKVPQLPG FSWIKPCLSP PNIVYIGLRD
210 220 230 240 250
VEPPEHFILK NYDIQYFSMR EIDRLGIQKV MEQTFDRLIG KRQRPIHLSF
260 270 280 290 300
DIDAFDPKLA PATGTPVVGG LTYREGVYIT EEIHNTGLLS ALDLVEVNPH
310 320 330 340 350
LATSEEEAKA TARLAVDVIA SSFGQTREGG HIVYDHLPTP SSPHESENEE

CVRI
Length:354
Mass (Da):38,878
Last modified:July 1, 1997 - v1
Checksum:iB372DF68A19473F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90886 mRNA. Translation: AAC22548.1.
AF032466 mRNA. Translation: AAB86959.1.
AF045965
, AF044680, AF045959, AF045960, AF045961, AF045962, AF045963, AF045964 Genomic DNA. Translation: AAC78460.1.
CCDSiCCDS26007.1.
RefSeqiNP_033835.1. NM_009705.3.
UniGeneiMm.3506.

Genome annotation databases

EnsembliENSMUST00000021550; ENSMUSP00000021550; ENSMUSG00000021125.
GeneIDi11847.
KEGGimmu:11847.
UCSCiuc007nzv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90886 mRNA. Translation: AAC22548.1.
AF032466 mRNA. Translation: AAB86959.1.
AF045965
, AF044680, AF045959, AF045960, AF045961, AF045962, AF045963, AF045964 Genomic DNA. Translation: AAC78460.1.
CCDSiCCDS26007.1.
RefSeqiNP_033835.1. NM_009705.3.
UniGeneiMm.3506.

3D structure databases

ProteinModelPortaliO08691.
SMRiO08691. Positions 24-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08691. 1 interaction.
MINTiMINT-4088250.
STRINGi10090.ENSMUSP00000021550.

PTM databases

PhosphoSiteiO08691.

2D gel databases

REPRODUCTION-2DPAGEO08691.

Proteomic databases

MaxQBiO08691.
PaxDbiO08691.
PRIDEiO08691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021550; ENSMUSP00000021550; ENSMUSG00000021125.
GeneIDi11847.
KEGGimmu:11847.
UCSCiuc007nzv.2. mouse.

Organism-specific databases

CTDi384.
MGIiMGI:1330806. Arg2.

Phylogenomic databases

eggNOGiCOG0010.
GeneTreeiENSGT00530000063082.
HOGENOMiHOG000204319.
HOVERGENiHBG003030.
InParanoidiO08691.
KOiK01476.
OMAiIATCFGQ.
OrthoDBiEOG747PJ5.
PhylomeDBiO08691.
TreeFamiTF300034.

Enzyme and pathway databases

UniPathwayiUPA00158; UER00270.
ReactomeiREACT_315699. Urea cycle.

Miscellaneous databases

NextBioi279811.
PROiO08691.
SOURCEiSearch...

Gene expression databases

BgeeiO08691.
CleanExiMM_ARG2.
GenevisibleiO08691. MM.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR014033. Arginase.
IPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PIRSFiPIRSF036979. Arginase. 1 hit.
PRINTSiPR00116. ARGINASE.
TIGRFAMsiTIGR01229. rocF_arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the mouse and rat type II arginase genes."
    Iyer R.K., Bando J.M., Jenkinson C.P., Vockley J.G., Kim P.S., Kern R.M., Cederbaum S.D., Grody W.W.
    Mol. Genet. Metab. 63:168-175(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells."
    Morris S.M. Jr., Kepka-Lenhart D., Chen L.C.
    Am. J. Physiol. 275:E740-E747(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 51-57, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiARGI2_MOUSE
AccessioniPrimary (citable) accession number: O08691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 24, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.