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O08689

- GDF8_MOUSE

UniProt

O08689 - GDF8_MOUSE

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Protein
Growth/differentiation factor 8
Gene
Mstn, Gdf8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts specifically as a negative regulator of skeletal muscle growth.

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. protein binding Source: MGI
  3. receptor binding Source: MGI

GO - Biological processi

  1. cellular response to dexamethasone stimulus Source: MGI
  2. negative regulation of muscle hypertrophy Source: Ensembl
  3. negative regulation of skeletal muscle tissue growth Source: Ensembl
  4. ovulation cycle process Source: Ensembl
  5. positive regulation of transcription, DNA-templated Source: Ensembl
  6. response to electrical stimulus Source: Ensembl
  7. response to estrogen Source: Ensembl
  8. response to ethanol Source: Ensembl
  9. response to gravity Source: Ensembl
  10. response to heat Source: Ensembl
  11. response to muscle activity Source: Ensembl
  12. response to testosterone Source: Ensembl
  13. skeletal muscle atrophy Source: Ensembl
  14. skeletal muscle tissue regeneration Source: Ensembl
  15. transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 8
Short name:
GDF-8
Alternative name(s):
Myostatin
Gene namesi
Name:Mstn
Synonyms:Gdf8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:95691. Mstn.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mutant animals exhibit muscle hypertrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Propeptidei25 – 267243 Reviewed prediction
PRO_0000033956Add
BLAST
Chaini268 – 376109Growth/differentiation factor 8
PRO_0000033957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi282 ↔ 3412 Publications
Disulfide bondi310 ↔ 3732 Publications
Disulfide bondi314 ↔ 3752 Publications
Disulfide bondi340 – 340Interchain2 Publications

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO08689.
PRIDEiO08689.

PTM databases

PhosphoSiteiO08689.

Expressioni

Tissue specificityi

Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue.

Developmental stagei

First detected 9.5 dpc in one-third of developing somites. At 10.5 dpc, expressed in the myotome compartment of somites. At later stages of development, detected in a wide range of developing muscles. Expression continues in adulthood.

Gene expression databases

ArrayExpressiO08689.
BgeeiO08689.
CleanExiMM_MSTN.
GenevestigatoriO08689.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity. Interacts with WFIKKN2, leading to inhibit its activity. Interacts with FST3 By similarity.3 Publications

Protein-protein interaction databases

BioGridi201535. 1 interaction.
IntActiO08689. 4 interactions.
MINTiMINT-7914794.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi281 – 2855
Beta strandi288 – 2903
Helixi291 – 2944
Beta strandi299 – 3013
Beta strandi303 – 3064
Beta strandi309 – 3113
Turni316 – 3194
Helixi324 – 3318
Beta strandi340 – 35415
Beta strandi360 – 37617

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HH2X-ray2.15A/B268-376[»]
3SEKX-ray2.40B268-376[»]
ProteinModelPortaliO08689.
SMRiO08689. Positions 50-376.

Miscellaneous databases

EvolutionaryTraceiO08689.

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG310007.
HOGENOMiHOG000006566.
HOVERGENiHBG000217.
InParanoidiO08689.
KOiK05497.
OMAiNPFLEVR.
OrthoDBiEOG74R1Q4.
PhylomeDBiO08689.
TreeFamiTF318514.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR015616. GDF_8.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PTHR11848:SF13. PTHR11848:SF13. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08689-1 [UniParc]FASTAAdd to Basket

« Hide

MMQKLQMYVY IYLFMLIAAG PVDLNEGSER EENVEKEGLC NACAWRQNTR    50
YSRIEAIKIQ ILSKLRLETA PNISKDAIRQ LLPRAPPLRE LIDQYDVQRD 100
DSSDGSLEDD DYHATTETII TMPTESDFLM QADGKPKCCF FKFSSKIQYN 150
KVVKAQLWIY LRPVKTPTTV FVQILRLIKP MKDGTRYTGI RSLKLDMSPG 200
TGIWQSIDVK TVLQNWLKQP ESNLGIEIKA LDENGHDLAV TFPGPGEDGL 250
NPFLEVKVTD TPKRSRRDFG LDCDEHSTES RCCRYPLTVD FEAFGWDWII 300
APKRYKANYC SGECEFVFLQ KYPHTHLVHQ ANPRGSAGPC CTPTKMSPIN 350
MLYFNGKEQI IYGKIPAMVV DRCGCS 376
Length:376
Mass (Da):42,921
Last modified:July 1, 1997 - v1
Checksum:i3E19814DD62C08BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U84005 mRNA. Translation: AAC53167.1.
CCDSiCCDS14950.1.
RefSeqiNP_034964.1. NM_010834.2.
UniGeneiMm.3514.

Genome annotation databases

EnsembliENSMUST00000027269; ENSMUSP00000027269; ENSMUSG00000026100.
GeneIDi17700.
KEGGimmu:17700.
UCSCiuc007ayt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U84005 mRNA. Translation: AAC53167.1 .
CCDSi CCDS14950.1.
RefSeqi NP_034964.1. NM_010834.2.
UniGenei Mm.3514.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3HH2 X-ray 2.15 A/B 268-376 [» ]
3SEK X-ray 2.40 B 268-376 [» ]
ProteinModelPortali O08689.
SMRi O08689. Positions 50-376.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201535. 1 interaction.
IntActi O08689. 4 interactions.
MINTi MINT-7914794.

PTM databases

PhosphoSitei O08689.

Proteomic databases

PaxDbi O08689.
PRIDEi O08689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027269 ; ENSMUSP00000027269 ; ENSMUSG00000026100 .
GeneIDi 17700.
KEGGi mmu:17700.
UCSCi uc007ayt.1. mouse.

Organism-specific databases

CTDi 2660.
MGIi MGI:95691. Mstn.

Phylogenomic databases

eggNOGi NOG310007.
HOGENOMi HOG000006566.
HOVERGENi HBG000217.
InParanoidi O08689.
KOi K05497.
OMAi NPFLEVR.
OrthoDBi EOG74R1Q4.
PhylomeDBi O08689.
TreeFami TF318514.

Miscellaneous databases

EvolutionaryTracei O08689.
NextBioi 292290.
PROi O08689.
SOURCEi Search...

Gene expression databases

ArrayExpressi O08689.
Bgeei O08689.
CleanExi MM_MSTN.
Genevestigatori O08689.

Family and domain databases

Gene3Di 2.10.90.10. 1 hit.
InterProi IPR029034. Cystine-knot_cytokine.
IPR015616. GDF_8.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view ]
PANTHERi PTHR11848. PTHR11848. 1 hit.
PTHR11848:SF13. PTHR11848:SF13. 1 hit.
Pfami PF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view ]
SMARTi SM00204. TGFB. 1 hit.
[Graphical view ]
SUPFAMi SSF57501. SSF57501. 1 hit.
PROSITEi PS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member."
    McPherron A.C., Lawler A.M., Lee S.-J.
    Nature 387:83-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Skeletal muscle.
  2. "Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains."
    Hill J.J., Qiu Y., Hewick R.M., Wolfman N.M.
    Mol. Endocrinol. 17:1144-1154(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WFIKKN2.
  3. Cited for: DISRUPTION PHENOTYPE.
  4. "The structure of myostatin:follistatin 288: insights into receptor utilization and heparin binding."
    Cash J.N., Rejon C.A., McPherron A.C., Bernard D.J., Thompson T.B.
    EMBO J. 28:2662-2676(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 268-376 IN COMPLEX WITH HUMAN FOLLISTATIN, HEPARIN-BINDING, DISULFIDE BONDS.
  5. "Structure of myostatin.follistatin-like 3: N-terminal domains of follistatin-type molecules exhibit alternate modes of binding."
    Cash J.N., Angerman E.B., Kattamuri C., Nolan K., Zhao H., Sidis Y., Keutmann H.T., Thompson T.B.
    J. Biol. Chem. 287:1043-1053(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 268-376 IN COMPLEX WITH HUMAN FSTL3, DISULFIDE BONDS.

Entry informationi

Entry nameiGDF8_MOUSE
AccessioniPrimary (citable) accession number: O08689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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