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O08688 (CAN5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calpain-5

EC=3.4.22.-
Alternative name(s):
New calpain 3
Short name=nCL-3
Gene names
Name:Capn5
Synonyms:Ncl3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Broad endopeptidase specificity.

Sequence similarities

Belongs to the peptidase C2 family.

Contains 1 C2 domain.

Contains 1 calpain catalytic domain.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell surface

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium-dependent cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Calpain-5
PRO_0000207714

Regions

Domain26 – 343318Calpain catalytic
Domain518 – 619102C2
Region344 – 496153Domain III

Sites

Active site811 By similarity
Active site2521 By similarity
Active site2841 By similarity

Natural variations

Natural variant4271D → N. Ref.2 Ref.4

Sequences

Sequence LengthMass (Da)Tools
O08688 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 7F1DA3E299FEC02D

FASTA64072,955
        10         20         30         40         50         60 
MFSCAKAYED QNYSALKRAC LRKKVLFEDP LFPATDDSLY YKGTPGPTVR WKRPKDICDD 

        70         80         90        100        110        120 
PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWN PEKPDSYAGI 

       130        140        150        160        170        180 
FHFNFWRFGE WVDVIVDDRL PTVNNQLIYC HSNSKNEFWC ALVEKAYAKL AGCYQALDGG 

       190        200        210        220        230        240 
NTADALVDFT GGVSEPIDLT EGDLATDEAK RNQLFERVLK VHSRGGLISA SIKAVTAADM 

       250        260        270        280        290        300 
EARLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WTGPWSDTSE 

       310        320        330        340        350        360 
EWQKVSKSER EKMGVTVQDD GEFWMTFEDM CRYFTDIIKC RLINTSYLSI HKTWEEARLH 

       370        380        390        400        410        420 
GAWTRHEDPQ QNRSGGCINH KDTFFQNPQY VFEVKKPEDE VLISIQQRPK RSTRREGKGE 

       430        440        450        460        470        480 
NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TELPEGRYVI IPTTFEPGHT 

       490        500        510        520        530        540 
GEFLLRVFTD VPSNCRELRL DEPPRTCWSS LCGYPQQVAQ VHVLGAAGLK DSPTGANSYV 

       550        560        570        580        590        600 
IIKCEGEKVR SAVQRGTSTP EYNVKGIFYR KKLAQPITVQ VWNHRVLKDE FLGQVHLKTA 

       610        620        630        640 
PDDLQDLHTL HLQDRSSRQP SDLPGIVAVR VLCSASLTAV 

« Hide

References

« Hide 'large scale' references
[1]"A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution."
Dear T.N., Matena K., Vingron M., Boehm T.
Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Genomic organization of mouse Capn5 and Capn6 genes confirms that they are a distinct calpain subfamily."
Matena K., Boehm T., Dear N.T.
Genomics 48:117-120(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-427.
[3]Algate P.A.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-427.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10656 mRNA. Translation: CAA71666.1.
U85020 mRNA. Translation: AAD00559.1.
BC014767 mRNA. Translation: AAH14767.1.
RefSeqNP_031628.1. NM_007602.3.
XP_006507339.1. XM_006507276.1.
UniGeneMm.326847.

3D structure databases

ProteinModelPortalO08688.
SMRO08688. Positions 5-490, 517-601.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC02.011.

PTM databases

PhosphoSiteO08688.

Proteomic databases

PaxDbO08688.
PRIDEO08688.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040971; ENSMUSP00000048183; ENSMUSG00000035547.
ENSMUST00000107112; ENSMUSP00000102729; ENSMUSG00000035547.
GeneID12337.
KEGGmmu:12337.
UCSCuc009ikc.1. mouse.

Organism-specific databases

CTD726.
MGIMGI:1100859. Capn5.

Phylogenomic databases

eggNOGNOG258770.
HOGENOMHOG000232036.
HOVERGENHBG001095.
InParanoidO08688.
KOK08574.
OMAPGPTVRW.
OrthoDBEOG75J0MJ.
PhylomeDBO08688.
TreeFamTF314748.

Gene expression databases

ArrayExpressO08688.
BgeeO08688.
CleanExMM_CAPN5.
GenevestigatorO08688.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
InterProIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamPF00168. C2. 1 hit.
PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSPR00704. CALPAIN.
SMARTSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280956.
PROO08688.
SOURCESearch...

Entry information

Entry nameCAN5_MOUSE
AccessionPrimary (citable) accession number: O08688
Secondary accession number(s): Q91YU0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot