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Protein

Calpain-5

Gene

Capn5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811By similarity
Active sitei252 – 2521By similarity
Active sitei284 – 2841By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.B25. 3474.

Protein family/group databases

MEROPSiC02.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-5 (EC:3.4.22.-)
Alternative name(s):
New calpain 3
Short name:
nCL-3
Gene namesi
Name:Capn5
Synonyms:Ncl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1100859. Capn5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Calpain-5PRO_0000207714Add
BLAST

Proteomic databases

MaxQBiO08688.
PaxDbiO08688.
PRIDEiO08688.

PTM databases

PhosphoSiteiO08688.

Expressioni

Gene expression databases

BgeeiO08688.
CleanExiMM_CAPN5.
ExpressionAtlasiO08688. baseline and differential.
GenevisibleiO08688. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048183.

Structurei

3D structure databases

ProteinModelPortaliO08688.
SMRiO08688. Positions 5-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 343318Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini518 – 619102C2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni344 – 496153Domain IIIAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 C2 domain.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG258770.
HOGENOMiHOG000232036.
HOVERGENiHBG001095.
InParanoidiO08688.
KOiK08574.
OMAiPGPTVRW.
OrthoDBiEOG75J0MJ.
PhylomeDBiO08688.
TreeFamiTF314748.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSCAKAYED QNYSALKRAC LRKKVLFEDP LFPATDDSLY YKGTPGPTVR
60 70 80 90 100
WKRPKDICDD PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV
110 120 130 140 150
IPDWKEQEWN PEKPDSYAGI FHFNFWRFGE WVDVIVDDRL PTVNNQLIYC
160 170 180 190 200
HSNSKNEFWC ALVEKAYAKL AGCYQALDGG NTADALVDFT GGVSEPIDLT
210 220 230 240 250
EGDLATDEAK RNQLFERVLK VHSRGGLISA SIKAVTAADM EARLACGLVK
260 270 280 290 300
GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WTGPWSDTSE
310 320 330 340 350
EWQKVSKSER EKMGVTVQDD GEFWMTFEDM CRYFTDIIKC RLINTSYLSI
360 370 380 390 400
HKTWEEARLH GAWTRHEDPQ QNRSGGCINH KDTFFQNPQY VFEVKKPEDE
410 420 430 440 450
VLISIQQRPK RSTRREGKGE NLAIGFDIYK VEENRQYRMH SLQHKAASSI
460 470 480 490 500
YINSRSVFLR TELPEGRYVI IPTTFEPGHT GEFLLRVFTD VPSNCRELRL
510 520 530 540 550
DEPPRTCWSS LCGYPQQVAQ VHVLGAAGLK DSPTGANSYV IIKCEGEKVR
560 570 580 590 600
SAVQRGTSTP EYNVKGIFYR KKLAQPITVQ VWNHRVLKDE FLGQVHLKTA
610 620 630 640
PDDLQDLHTL HLQDRSSRQP SDLPGIVAVR VLCSASLTAV
Length:640
Mass (Da):72,955
Last modified:July 1, 1997 - v1
Checksum:i7F1DA3E299FEC02D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti427 – 4271D → N.2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10656 mRNA. Translation: CAA71666.1.
U85020 mRNA. Translation: AAD00559.1.
BC014767 mRNA. Translation: AAH14767.1.
CCDSiCCDS21465.1.
RefSeqiNP_001288179.1. NM_001301250.1.
NP_031628.1. NM_007602.4.
UniGeneiMm.326847.

Genome annotation databases

EnsembliENSMUST00000040971; ENSMUSP00000048183; ENSMUSG00000035547.
ENSMUST00000107112; ENSMUSP00000102729; ENSMUSG00000035547.
GeneIDi12337.
KEGGimmu:12337.
UCSCiuc009ikc.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10656 mRNA. Translation: CAA71666.1.
U85020 mRNA. Translation: AAD00559.1.
BC014767 mRNA. Translation: AAH14767.1.
CCDSiCCDS21465.1.
RefSeqiNP_001288179.1. NM_001301250.1.
NP_031628.1. NM_007602.4.
UniGeneiMm.326847.

3D structure databases

ProteinModelPortaliO08688.
SMRiO08688. Positions 5-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048183.

Protein family/group databases

MEROPSiC02.011.

PTM databases

PhosphoSiteiO08688.

Proteomic databases

MaxQBiO08688.
PaxDbiO08688.
PRIDEiO08688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040971; ENSMUSP00000048183; ENSMUSG00000035547.
ENSMUST00000107112; ENSMUSP00000102729; ENSMUSG00000035547.
GeneIDi12337.
KEGGimmu:12337.
UCSCiuc009ikc.1. mouse.

Organism-specific databases

CTDi726.
MGIiMGI:1100859. Capn5.

Phylogenomic databases

eggNOGiNOG258770.
HOGENOMiHOG000232036.
HOVERGENiHBG001095.
InParanoidiO08688.
KOiK08574.
OMAiPGPTVRW.
OrthoDBiEOG75J0MJ.
PhylomeDBiO08688.
TreeFamiTF314748.

Enzyme and pathway databases

BRENDAi3.4.22.B25. 3474.

Miscellaneous databases

ChiTaRSiCapn5. mouse.
NextBioi280956.
PROiO08688.
SOURCEiSearch...

Gene expression databases

BgeeiO08688.
CleanExiMM_CAPN5.
ExpressionAtlasiO08688. baseline and differential.
GenevisibleiO08688. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution."
    Dear T.N., Matena K., Vingron M., Boehm T.
    Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Genomic organization of mouse Capn5 and Capn6 genes confirms that they are a distinct calpain subfamily."
    Matena K., Boehm T., Dear N.T.
    Genomics 48:117-120(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-427.
  3. Algate P.A.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-427.

Entry informationi

Entry nameiCAN5_MOUSE
AccessioniPrimary (citable) accession number: O08688
Secondary accession number(s): Q91YU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: July 1, 1997
Last modified: June 24, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.