Reviewed, UniProtKB/Swiss-Prot O08686 (BARX2_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein BarH-like 2

Gene names

Name:

Barx2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia.
Subcellular location	Nucleus.
Tissue specificity	Nervous system, particularly in the telencephalon, spinal cord, and dorsal root ganglia.
Developmental stage	Highly expressed in small groups of cells undergoing tissue remodeling, such as ectodermal cells within indentations surrounding the eye and maxillo-nasal groove and in the first branchial pouch, lung buds, precartilagenous condensations, and mesenchyme of the limb. At E9.5, expressed only to head, prominent in the region of telencephalon and mesencephalon, and concentrated in cells along the dorsal midline. At E10.5, expression is moderated in the most rostral regions of the head, but particularly prominent in a lateral band of cells in the periocular region. At E11.5, detected in the telencephalon, frontonasal region and limbs. At E12.5, expressed in the telencephalon and hindbrain.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the BAR homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cartilage condensation Inferred from direct assay. Source: MGI catagen Inferred from mutant phenotype. Source: MGI myotube differentiation Ref.3 Inferred from mutant phenotype. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	chromatin binding Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI sequence-specific DNA binding Inferred from direct assay. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Homeobox protein BarH-like 2		PRO_0000048839
Regions
	DNA binding	139 – 198	60	Homeobox
	Compositional bias	103 – 112	10	Poly-Ala
Amino acid modifications
	Modified residue	241	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	73	1	L → P in AAB53146. Ref.4
	Sequence conflict	105	1	A → S in AAH12684. Ref.2
	Sequence conflict	106	1	E → EA in AAH12684. Ref.2
	Sequence conflict	244 – 273	30	Missing in AAB53146. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O08686-1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: BCD8CC756B820953	FASTA	283	31,534
	10 20 30 40 50 60 MHCHAELRLS SPGQLKAARR RYKTFMIDEI LSKETCDYFE KLSLYSVCPS LVVRPKPLHS 70 80 90 100 110 120 CTGSPSLRAY PLLSVITRQP TVISHLVPTG SGLTPVLTRH PVAAAEAAAA AAETPGGEAL 130 140 150 160 170 180 ASSESETEQP TPRQKKPRRS RTIFTELQLM GLEKKFQKQK YLSTPDRLDL AQSLGLTQLQ 190 200 210 220 230 240 VKTWYQNRRM KWKKMVLKGG QEAPTKPKGR PKKNSIPTSE EIEAEEKMNS QAQSQELLES 250 260 270 280 SERQEEPCDT QEPKACLVPL EVAEPIHQPQ ELSEASSEPP PLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[3]	"The homeodomain protein Barx2 promotes myogenic differentiation and is regulated by myogenic regulatory factors." Meech R., Makarenkova H., Edelman D.B., Jones F.S. J. Biol. Chem. 278:8269-8278(2003) [PubMed: 12486129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62. Strain: 129/SvJ.
[4]	"Barx2, a new homeobox gene of the Bar class, is expressed in neural and craniofacial structures during development." Jones F.S., Kioussi C., Copertino D.W., Kallunki P., Holst B.D., Edelman G.M. Proc. Natl. Acad. Sci. U.S.A. 94:2632-2637(1997) [PubMed: 9122247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-283. Strain: BALB/c. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT030724 Genomic DNA. No translation available. BC012684 mRNA. Translation: AAH12684.1. Different initiation. AY188085 Genomic DNA. Translation: AAO25649.1. L77900 mRNA. Translation: AAB53146.1. Different initiation.
IPI	IPI00890009.
PIR	JC6307.
RefSeq	NP_038828.2.
UniGene	Mm.261044
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O08686.
PTM databases
PhosphoSite	O08686.
Proteomic databases
PRIDE	O08686.
Genome annotation databases
Ensembl	ENSMUST00000051296; ENSMUSP00000053945; ENSMUSG00000032033; Mus musculus. [Genome view]
GeneID	12023.
KEGG	mmu:12023.
Organism-specific databases
CTD	12023.
MGI	MGI:109617. Barx2.
Phylogenomic databases
eggNOG	roNOG07690.
HOGENOM	HBG717712.
HOVERGEN	O08686.
Gene expression databases
ArrayExpress	O08686.
Bgee	O08686.
CleanEx	MM_BARX2.
Genevestigator	O08686.
GermOnline	ENSMUSG00000032033. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_region. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR000047. HTH_lambrepressr. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

BARX2_MOUSE

Accession

Primary (citable) accession number: O08686
Secondary accession number(s): Q80ZF9, Q921G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	July 28, 2009
Last modified:	January 19, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)