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Protein

Homeobox protein BarH-like 2

Gene

Barx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi139 – 19860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cartilage condensation Source: MGI
  • myotube differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein BarH-like 2
Gene namesi
Name:Barx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:109617. Barx2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Homeobox protein BarH-like 2PRO_0000048839Add
BLAST

Proteomic databases

MaxQBiO08686.
PaxDbiO08686.
PRIDEiO08686.

PTM databases

iPTMnetiO08686.
PhosphoSiteiO08686.

Expressioni

Tissue specificityi

Nervous system, particularly in the telencephalon, spinal cord, and dorsal root ganglia.

Developmental stagei

Highly expressed in small groups of cells undergoing tissue remodeling, such as ectodermal cells within indentations surrounding the eye and maxillo-nasal groove and in the first branchial pouch, lung buds, precartilagenous condensations, and mesenchyme of the limb. At E9.5, expressed only to head, prominent in the region of telencephalon and mesencephalon, and concentrated in cells along the dorsal midline. At E10.5, expression is moderated in the most rostral regions of the head, but particularly prominent in a lateral band of cells in the periocular region. At E11.5, detected in the telencephalon, frontonasal region and limbs. At E12.5, expressed in the telencephalon and hindbrain.

Gene expression databases

BgeeiO08686.
CleanExiMM_BARX2.
GenevisibleiO08686. MM.

Interactioni

Protein-protein interaction databases

BioGridi198303. 7 interactions.
IntActiO08686. 3 interactions.
STRINGi10090.ENSMUSP00000112314.

Structurei

3D structure databases

ProteinModelPortaliO08686.
SMRiO08686. Positions 129-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 11210Poly-Ala

Sequence similaritiesi

Belongs to the BAR homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0488. Eukaryota.
ENOG411188D. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000231924.
HOVERGENiHBG050665.
InParanoidiO08686.
KOiK09361.
OMAiLHSCTGP.
OrthoDBiEOG71G9VZ.
PhylomeDBiO08686.
TreeFamiTF350735.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCHAELRLS SPGQLKAARR RYKTFMIDEI LSKETCDYFE KLSLYSVCPS
60 70 80 90 100
LVVRPKPLHS CTGSPSLRAY PLLSVITRQP TVISHLVPTG SGLTPVLTRH
110 120 130 140 150
PVAAAEAAAA AAETPGGEAL ASSESETEQP TPRQKKPRRS RTIFTELQLM
160 170 180 190 200
GLEKKFQKQK YLSTPDRLDL AQSLGLTQLQ VKTWYQNRRM KWKKMVLKGG
210 220 230 240 250
QEAPTKPKGR PKKNSIPTSE EIEAEEKMNS QAQSQELLES SERQEEPCDT
260 270 280
QEPKACLVPL EVAEPIHQPQ ELSEASSEPP PLS
Length:283
Mass (Da):31,534
Last modified:July 28, 2009 - v2
Checksum:iBCD8CC756B820953
GO

Sequence cautioni

The sequence AAB53146.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH12684.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731L → P in AAB53146 (PubMed:9122247).Curated
Sequence conflicti105 – 1051A → S in AAH12684 (PubMed:15489334).Curated
Sequence conflicti106 – 1061E → EA in AAH12684 (PubMed:15489334).Curated
Sequence conflicti244 – 27330Missing in AAB53146 (PubMed:9122247).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT030724 Genomic DNA. No translation available.
BC012684 mRNA. Translation: AAH12684.1. Different initiation.
AY188085 Genomic DNA. Translation: AAO25649.1.
L77900 mRNA. Translation: AAB53146.1. Different initiation.
CCDSiCCDS52750.1.
PIRiJC6307.
RefSeqiNP_038828.2. NM_013800.2.
UniGeneiMm.261044.

Genome annotation databases

EnsembliENSMUST00000116615; ENSMUSP00000112314; ENSMUSG00000032033.
GeneIDi12023.
KEGGimmu:12023.
UCSCiuc009oru.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT030724 Genomic DNA. No translation available.
BC012684 mRNA. Translation: AAH12684.1. Different initiation.
AY188085 Genomic DNA. Translation: AAO25649.1.
L77900 mRNA. Translation: AAB53146.1. Different initiation.
CCDSiCCDS52750.1.
PIRiJC6307.
RefSeqiNP_038828.2. NM_013800.2.
UniGeneiMm.261044.

3D structure databases

ProteinModelPortaliO08686.
SMRiO08686. Positions 129-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198303. 7 interactions.
IntActiO08686. 3 interactions.
STRINGi10090.ENSMUSP00000112314.

PTM databases

iPTMnetiO08686.
PhosphoSiteiO08686.

Proteomic databases

MaxQBiO08686.
PaxDbiO08686.
PRIDEiO08686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116615; ENSMUSP00000112314; ENSMUSG00000032033.
GeneIDi12023.
KEGGimmu:12023.
UCSCiuc009oru.1. mouse.

Organism-specific databases

CTDi8538.
MGIiMGI:109617. Barx2.

Phylogenomic databases

eggNOGiKOG0488. Eukaryota.
ENOG411188D. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000231924.
HOVERGENiHBG050665.
InParanoidiO08686.
KOiK09361.
OMAiLHSCTGP.
OrthoDBiEOG71G9VZ.
PhylomeDBiO08686.
TreeFamiTF350735.

Miscellaneous databases

ChiTaRSiBarx2. mouse.
PROiO08686.
SOURCEiSearch...

Gene expression databases

BgeeiO08686.
CleanExiMM_BARX2.
GenevisibleiO08686. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "The homeodomain protein Barx2 promotes myogenic differentiation and is regulated by myogenic regulatory factors."
    Meech R., Makarenkova H., Edelman D.B., Jones F.S.
    J. Biol. Chem. 278:8269-8278(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
    Strain: 129/SvJ.
  4. "Barx2, a new homeobox gene of the Bar class, is expressed in neural and craniofacial structures during development."
    Jones F.S., Kioussi C., Copertino D.W., Kallunki P., Holst B.D., Edelman G.M.
    Proc. Natl. Acad. Sci. U.S.A. 94:2632-2637(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-283.
    Strain: BALB/cJ.
    Tissue: Embryo.

Entry informationi

Entry nameiBARX2_MOUSE
AccessioniPrimary (citable) accession number: O08686
Secondary accession number(s): Q80ZF9, Q921G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 28, 2009
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.