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Reviewed, UniProtKB/Swiss-Prot O08684 (PLD1_CRIGR)

Last modified October 13, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phospholipase D1
      Short name=PLD 1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D1
Gene names
Name: PLD1
OrganismCricetulus griseus (Chinese hamster)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic By similarity.

Catalytic activity

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulation

Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42 By similarity.

Subunit structure

Interacts with PIP5K1A By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side By similarity. Late endosome membrane; Lipid-anchor; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the phospholipase D family.

Contains 1 PH domain.

Contains 2 PLD phosphodiesterase domains.

Contains 1 PX (phox homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10361036Phospholipase D1
PRO_0000218801

Regions

Domain81 – 212132PX
Domain219 – 328110PH
Domain459 – 48628PLD phosphodiesterase 1
Domain853 – 88028PLD phosphodiesterase 2
Region463 – 890428Catalytic

Amino acid modifications

Modified residue5911Phosphoserine By similarity
Lipidation2401S-palmitoyl cysteine By similarity
Lipidation2411S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
O08684-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 342A561E2BBFE51E

FASTA1,036119,515
        10         20         30         40         50         60 
MSLKSEHRVN TSALQKIAAD MSNLIDNLDT RELHFEGEEV EFDTSPGDPK AQEKYIPFSS 

        70         80         90        100        110        120 
IYNTQGFKEP NIQTYLSGCP IKAQVLEVER FTSTTRVPSI NLYTIELTHG EFTWQVKRKF 

       130        140        150        160        170        180 
KHFQEFHREL LKYKAFIRIP IPTKRHTFRR QNVKEEPREM PSLPRSSENT IQEEQFFGRR 

       190        200        210        220        230        240 
KQLEDYLTKI LKMPMYRNYH ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGLNC 

       250        260        270        280        290        300 
CGQGRACYRW SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRIKVGRKE TETKYGLRID 

       310        320        330        340        350        360 
NLSRTLILKC NSYRHARWWG GAIEEFIQKH GSDFLKDHRF GSYAAVHENM LAKWYVNAKG 

       370        380        390        400        410        420 
YFEDIANAME EAAEEIFITD WWLSPEIFLK RPVVEGNRWR LDCILKRKAQ QGVRIFIMLY 

       430        440        450        460        470        480 
KEVELALGIN SEYSKRTLMR LHPNIKVMRH PDHVSSSVYL WAHHEKLVII DQSVAFVGGI 

       490        500        510        520        530        540 
DLAYGRWDDN EHRLTDVGSV KRVTSGLSMG SLAAATMESM ESLSLKDNHR SHKNEPILKS 

       550        560        570        580        590        600 
VDDVDPKLKG VGKPRKFSKF SLYRQLHRRH LHNSDSVSSI DSASNTGSIR SVQTGVGELH 

       610        620        630        640        650        660 
GETRFWHGKD YCNFVFKDWV QLDKPFADFI DRYSTPRIPW HDIGSVLHGK AARDVARHFI 

       670        680        690        700        710        720 
QRWNFTKIMK PKYRSLSYPF LLPKSQSTAH ELRYQVPGAV PAKVQLLRSA ADWSAGIKHH 

       730        740        750        760        770        780 
EESIHSAYIN VIENSKHYIY IENQFFISCA DDKVVFNKVG DAIAQRILKA HREGQRYRVY 

       790        800        810        820        830        840 
IVIPRLPGFE GDISTGGGNA LQAIMHFNYR TMCRGENSIL GQLKPELGNQ WINYISFCGL 

       850        860        870        880        890        900 
RTHAELEGNL VTELIYVHSK LLIADDNTVI IGSANINDRS MLGKRDSEMA VIVQDTETVP 

       910        920        930        940        950        960 
SIMDGKEYQA GCFAQGLRLQ CFRLVLGYLS DPSEDLQDPV SDKFFKEIWV STAARNATIY 

       970        980        990       1000       1010       1020 
DKVFRCLPND EVHNLMQLRD FISKPILAKD DPIRAEEELR KIRGFLVQFP FYFLSEENLL 

      1030 
PSVGTKEAIV PMEVWT 

« Hide

References

[1]Klaus J.R., Baldassare J.J., Raben D.M.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

U94995 mRNA. Translation: AAB53631.1.
PIRT18530.

3D structure databases

ModBaseSearch...

Phylogenomic databases

HOVERGENO08684.

Enzyme and pathway databases

BRENDA3.1.4.4. 18.

Family and domain databases

InterProIPR011993. PH_type.
IPR015679. Phospholipase_D.
IPR001849. Pleckstrin_homology.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D_euk.
IPR001683. PX.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:3.30.1520.10. PX. 1 hit.
PANTHERPTHR18896. Phospholipase_D. 1 hit.
PfamPF00169. PH. 1 hit.
PF00614. PLDc. 2 hits.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. False negative.
PS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLD1_CRIGR
AccessionPrimary (citable) accession number: O08684
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 1, 1997
Last modified: October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents