Reviewed, UniProtKB/Swiss-Prot O08684 (PLD1_CRIGR)

Names and origin

Protein names

Recommended name:
    Phospholipase D1
      Short name=PLD 1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D1

Gene names

Name:

PLD1

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	1036 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic By similarity.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42 By similarity.
Subunit structure	Interacts with PIP5K1A By similarity.
Subcellular location	Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side By similarity. Late endosome membrane; Lipid-anchor; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Repeat
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phosphoinositide binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1036	1036	Phospholipase D1		PRO_0000218801
Regions
	Domain	81 – 212	132	PX
	Domain	219 – 328	110	PH
	Domain	459 – 486	28	PLD phosphodiesterase 1
	Domain	853 – 880	28	PLD phosphodiesterase 2
	Region	463 – 890	428	Catalytic
Amino acid modifications
	Modified residue	591	1	Phosphoserine By similarity
	Lipidation	240	1	S-palmitoyl cysteine By similarity
	Lipidation	241	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08684-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 342A561E2BBFE51E	FASTA	1,036	119,515
	10 20 30 40 50 60 MSLKSEHRVN TSALQKIAAD MSNLIDNLDT RELHFEGEEV EFDTSPGDPK AQEKYIPFSS 70 80 90 100 110 120 IYNTQGFKEP NIQTYLSGCP IKAQVLEVER FTSTTRVPSI NLYTIELTHG EFTWQVKRKF 130 140 150 160 170 180 KHFQEFHREL LKYKAFIRIP IPTKRHTFRR QNVKEEPREM PSLPRSSENT IQEEQFFGRR 190 200 210 220 230 240 KQLEDYLTKI LKMPMYRNYH ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGLNC 250 260 270 280 290 300 CGQGRACYRW SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRIKVGRKE TETKYGLRID 310 320 330 340 350 360 NLSRTLILKC NSYRHARWWG GAIEEFIQKH GSDFLKDHRF GSYAAVHENM LAKWYVNAKG 370 380 390 400 410 420 YFEDIANAME EAAEEIFITD WWLSPEIFLK RPVVEGNRWR LDCILKRKAQ QGVRIFIMLY 430 440 450 460 470 480 KEVELALGIN SEYSKRTLMR LHPNIKVMRH PDHVSSSVYL WAHHEKLVII DQSVAFVGGI 490 500 510 520 530 540 DLAYGRWDDN EHRLTDVGSV KRVTSGLSMG SLAAATMESM ESLSLKDNHR SHKNEPILKS 550 560 570 580 590 600 VDDVDPKLKG VGKPRKFSKF SLYRQLHRRH LHNSDSVSSI DSASNTGSIR SVQTGVGELH 610 620 630 640 650 660 GETRFWHGKD YCNFVFKDWV QLDKPFADFI DRYSTPRIPW HDIGSVLHGK AARDVARHFI 670 680 690 700 710 720 QRWNFTKIMK PKYRSLSYPF LLPKSQSTAH ELRYQVPGAV PAKVQLLRSA ADWSAGIKHH 730 740 750 760 770 780 EESIHSAYIN VIENSKHYIY IENQFFISCA DDKVVFNKVG DAIAQRILKA HREGQRYRVY 790 800 810 820 830 840 IVIPRLPGFE GDISTGGGNA LQAIMHFNYR TMCRGENSIL GQLKPELGNQ WINYISFCGL 850 860 870 880 890 900 RTHAELEGNL VTELIYVHSK LLIADDNTVI IGSANINDRS MLGKRDSEMA VIVQDTETVP 910 920 930 940 950 960 SIMDGKEYQA GCFAQGLRLQ CFRLVLGYLS DPSEDLQDPV SDKFFKEIWV STAARNATIY 970 980 990 1000 1010 1020 DKVFRCLPND EVHNLMQLRD FISKPILAKD DPIRAEEELR KIRGFLVQFP FYFLSEENLL 1030 PSVGTKEAIV PMEVWT

References

[1]	Klaus J.R., Baldassare J.J., Raben D.M. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U94995 mRNA. Translation: AAB53631.1.
PIR	T18530.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	O08684.
Enzyme and pathway databases
BRENDA	3.1.4.4. 18.
Family and domain databases
InterPro	IPR011993. PH_type. IPR015679. Phospholipase_D. IPR001849. Pleckstrin_homology. IPR001736. PLipase_D/transphosphatidylase. IPR016555. PLipase_D_euk. IPR001683. PX. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.1520.10. PX. 1 hit.
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00614. PLDc. 2 hits. PF00787. PX. 1 hit. [Graphical view]
PIRSF	PIRSF009376. Phospholipase_D_euk. 1 hit.
SMART	SM00233. PH. 1 hit. SM00155. PLDc. 2 hits. SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. PS50035. PLD. 2 hits. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLD1_CRIGR

Accession

Primary (citable) accession number: O08684

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)