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Protein

Proteinase-activated receptor 3

Gene

F2rl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-456926. Thrombin signalling through proteinase activated receptors (PARs).

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 3
Short name:
PAR-3
Alternative name(s):
Coagulation factor II receptor-like 2
Thrombin receptor-like 2
Gene namesi
Name:F2rl2
Synonyms:Par3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1298208. F2rl2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 93ExtracellularSequence analysisAdd BLAST56
Transmembranei94 – 119Helical; Name=1Sequence analysisAdd BLAST26
Topological domaini120 – 127CytoplasmicSequence analysis8
Transmembranei128 – 147Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini148 – 166ExtracellularSequence analysisAdd BLAST19
Transmembranei167 – 188Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini189 – 205CytoplasmicSequence analysisAdd BLAST17
Transmembranei206 – 229Helical; Name=4Sequence analysisAdd BLAST24
Topological domaini230 – 259ExtracellularSequence analysisAdd BLAST30
Transmembranei260 – 279Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini280 – 296CytoplasmicSequence analysisAdd BLAST17
Transmembranei297 – 321Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini322 – 335ExtracellularSequence analysisAdd BLAST14
Transmembranei336 – 360Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini361 – 369CytoplasmicSequence analysis9

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000001275820 – 37Removed for receptor activationBy similarityAdd BLAST18
ChainiPRO_000001275938 – 369Proteinase-activated receptor 3Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi165 ↔ 244PROSITE-ProRule annotation

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei37 – 38Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08675.
PaxDbiO08675.
PRIDEiO08675.

PTM databases

iPTMnetiO08675.
PhosphoSitePlusiO08675.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021675.
CleanExiMM_F2RL2.
GenevisibleiO08675. MM.

Interactioni

Subunit structurei

Interacts with INSC/inscuteable and GPSM2.1 Publication

Protein-protein interaction databases

DIPiDIP-41474N.
MINTiMINT-261941.
STRINGi10090.ENSMUSP00000022182.

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi52 – 54Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PUXX-ray2.00C44-56[»]
ProteinModelPortaliO08675.
SMRiO08675.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08675.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJUX. Eukaryota.
ENOG4111GWA. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000116291.
HOVERGENiHBG105658.
InParanoidiO08675.
KOiK04235.
OMAiSAIEGWT.
OrthoDBiEOG091G0C2F.
TreeFamiTF330775.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003943. Prot_act_rcpt_3.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 2 hits.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01429. PROTEASEAR3.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILILVAAG LLFLPVTVCQ SGINVSDNSA KPTLTIKSFN GGPQNTFEEF
60 70 80 90 100
PLSDIEGWTG ATTTIKAECP EDSISTLHVN NATIGYLRSS LSTQVIPAIY
110 120 130 140 150
ILLFVVGVPA NIVTLWKLSL RTKSISLVIF HTNLAIADLL FCVTLPFKIA
160 170 180 190 200
YHLNGNNWVF GEVTCRITTV VFYGNMYCAI LILTCMGINR YLATAHPFTY
210 220 230 240 250
QKLPKRSFSM LMCGMVWVMV FLYMLPFVIL KQEYHLVHSE ITTCHDVVDA
260 270 280 290 300
CESPSSFRFY YFVSLAFFGF LIPFVIIIFC YTTLIHKLKS KDRIWLGYIK
310 320 330 340 350
AVLLILVIFT ICFAPTNIIL VIHHANYYYH NTDSLYFMYL IALCLGSLNS
360
CLDPFLYFVM SKVVDQLNP
Length:369
Mass (Da):41,697
Last modified:July 27, 2011 - v2
Checksum:iC8E6B0097F0BF90F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110A → S in AAC53137 (PubMed:9087410).Curated1
Sequence conflicti110A → S in AAI40986 (PubMed:15489334).Curated1
Sequence conflicti164T → M in AAC53137 (PubMed:9087410).Curated1
Sequence conflicti164T → M in AAI40986 (PubMed:15489334).Curated1
Sequence conflicti210M → L in AAC53137 (PubMed:9087410).Curated1
Sequence conflicti210M → L in AAI40986 (PubMed:15489334).Curated1
Sequence conflicti215M → I in AAC53137 (PubMed:9087410).Curated1
Sequence conflicti215M → I in AAI40986 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92972 mRNA. Translation: AAC53137.1.
AK135212 mRNA. Translation: BAE22460.1.
BC140985 mRNA. Translation: AAI40986.1.
CCDSiCCDS26702.1.
RefSeqiNP_034300.3. NM_010170.4.
UniGeneiMm.440952.

Genome annotation databases

EnsembliENSMUST00000022182; ENSMUSP00000022182; ENSMUSG00000021675.
GeneIDi14064.
KEGGimmu:14064.
UCSCiuc007rmq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92972 mRNA. Translation: AAC53137.1.
AK135212 mRNA. Translation: BAE22460.1.
BC140985 mRNA. Translation: AAI40986.1.
CCDSiCCDS26702.1.
RefSeqiNP_034300.3. NM_010170.4.
UniGeneiMm.440952.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PUXX-ray2.00C44-56[»]
ProteinModelPortaliO08675.
SMRiO08675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41474N.
MINTiMINT-261941.
STRINGi10090.ENSMUSP00000022182.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiO08675.
PhosphoSitePlusiO08675.

Proteomic databases

MaxQBiO08675.
PaxDbiO08675.
PRIDEiO08675.

Protocols and materials databases

DNASUi14064.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022182; ENSMUSP00000022182; ENSMUSG00000021675.
GeneIDi14064.
KEGGimmu:14064.
UCSCiuc007rmq.1. mouse.

Organism-specific databases

CTDi2151.
MGIiMGI:1298208. F2rl2.

Phylogenomic databases

eggNOGiENOG410IJUX. Eukaryota.
ENOG4111GWA. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000116291.
HOVERGENiHBG105658.
InParanoidiO08675.
KOiK04235.
OMAiSAIEGWT.
OrthoDBiEOG091G0C2F.
TreeFamiTF330775.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-456926. Thrombin signalling through proteinase activated receptors (PARs).

Miscellaneous databases

EvolutionaryTraceiO08675.
PROiO08675.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021675.
CleanExiMM_F2RL2.
GenevisibleiO08675. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003943. Prot_act_rcpt_3.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 2 hits.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01429. PROTEASEAR3.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR3_MOUSE
AccessioniPrimary (citable) accession number: O08675
Secondary accession number(s): B9EIT2, Q3UXV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.