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O08675

- PAR3_MOUSE

UniProt

O08675 - PAR3_MOUSE

Protein

Proteinase-activated receptor 3

Gene

F2rl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei37 – 382Cleavage; by thrombinBy similarity

    GO - Molecular functioni

    1. thrombin receptor activity Source: InterPro

    GO - Biological processi

    1. blood coagulation Source: UniProtKB-KW

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteinase-activated receptor 3
    Short name:
    PAR-3
    Alternative name(s):
    Coagulation factor II receptor-like 2
    Thrombin receptor-like 2
    Gene namesi
    Name:F2rl2
    Synonyms:Par3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1298208. F2rl2.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 3718Removed for receptor activationBy similarityPRO_0000012758Add
    BLAST
    Chaini38 – 369332Proteinase-activated receptor 3PRO_0000012759Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi165 ↔ 244PROSITE-ProRule annotation

    Post-translational modificationi

    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO08675.

    PTM databases

    PhosphoSiteiO08675.

    Expressioni

    Gene expression databases

    BgeeiO08675.
    CleanExiMM_F2RL2.
    GenevestigatoriO08675.

    Interactioni

    Subunit structurei

    Interacts with INSC/inscuteable and GPSM2.1 Publication

    Protein-protein interaction databases

    DIPiDIP-41474N.
    MINTiMINT-261941.

    Structurei

    Secondary structure

    1
    369
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi52 – 543

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PUXX-ray2.00C44-56[»]
    ProteinModelPortaliO08675.
    SMRiO08675. Positions 85-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO08675.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini38 – 9356ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini120 – 1278CytoplasmicSequence Analysis
    Topological domaini148 – 16619ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini189 – 20517CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini230 – 25930ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini280 – 29617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini322 – 33514ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini361 – 3699CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei94 – 11926Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei128 – 14720Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei167 – 18822Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei206 – 22924Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei260 – 27920Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei297 – 32125Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei336 – 36025Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG146611.
    GeneTreeiENSGT00660000095329.
    HOGENOMiHOG000116291.
    HOVERGENiHBG105658.
    InParanoidiQ3UXV3.
    KOiK04235.
    OMAiCPEDSIS.
    OrthoDBiEOG7QC7WD.
    TreeFamiTF330775.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003943. Prot_act_rcpt_3.
    IPR003912. Protea_act_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR01428. PROTEASEAR.
    PR01429. PROTEASEAR3.
    PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08675-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILILVAAG LLFLPVTVCQ SGINVSDNSA KPTLTIKSFN GGPQNTFEEF    50
    PLSDIEGWTG ATTTIKAECP EDSISTLHVN NATIGYLRSS LSTQVIPAIY 100
    ILLFVVGVPA NIVTLWKLSL RTKSISLVIF HTNLAIADLL FCVTLPFKIA 150
    YHLNGNNWVF GEVTCRITTV VFYGNMYCAI LILTCMGINR YLATAHPFTY 200
    QKLPKRSFSM LMCGMVWVMV FLYMLPFVIL KQEYHLVHSE ITTCHDVVDA 250
    CESPSSFRFY YFVSLAFFGF LIPFVIIIFC YTTLIHKLKS KDRIWLGYIK 300
    AVLLILVIFT ICFAPTNIIL VIHHANYYYH NTDSLYFMYL IALCLGSLNS 350
    CLDPFLYFVM SKVVDQLNP 369
    Length:369
    Mass (Da):41,697
    Last modified:July 27, 2011 - v2
    Checksum:iC8E6B0097F0BF90F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101A → S in AAC53137. (PubMed:9087410)Curated
    Sequence conflicti110 – 1101A → S in AAI40986. (PubMed:15489334)Curated
    Sequence conflicti164 – 1641T → M in AAC53137. (PubMed:9087410)Curated
    Sequence conflicti164 – 1641T → M in AAI40986. (PubMed:15489334)Curated
    Sequence conflicti210 – 2101M → L in AAC53137. (PubMed:9087410)Curated
    Sequence conflicti210 – 2101M → L in AAI40986. (PubMed:15489334)Curated
    Sequence conflicti215 – 2151M → I in AAC53137. (PubMed:9087410)Curated
    Sequence conflicti215 – 2151M → I in AAI40986. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92972 mRNA. Translation: AAC53137.1.
    AK135212 mRNA. Translation: BAE22460.1.
    BC140985 mRNA. Translation: AAI40986.1.
    CCDSiCCDS26702.1.
    RefSeqiNP_034300.3. NM_010170.4.
    UniGeneiMm.440952.

    Genome annotation databases

    EnsembliENSMUST00000022182; ENSMUSP00000022182; ENSMUSG00000021675.
    GeneIDi14064.
    KEGGimmu:14064.
    UCSCiuc007rmq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92972 mRNA. Translation: AAC53137.1 .
    AK135212 mRNA. Translation: BAE22460.1 .
    BC140985 mRNA. Translation: AAI40986.1 .
    CCDSi CCDS26702.1.
    RefSeqi NP_034300.3. NM_010170.4.
    UniGenei Mm.440952.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PUX X-ray 2.00 C 44-56 [» ]
    ProteinModelPortali O08675.
    SMRi O08675. Positions 85-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-41474N.
    MINTi MINT-261941.

    Chemistry

    GuidetoPHARMACOLOGYi 349.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei O08675.

    Proteomic databases

    PRIDEi O08675.

    Protocols and materials databases

    DNASUi 14064.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022182 ; ENSMUSP00000022182 ; ENSMUSG00000021675 .
    GeneIDi 14064.
    KEGGi mmu:14064.
    UCSCi uc007rmq.1. mouse.

    Organism-specific databases

    CTDi 2151.
    MGIi MGI:1298208. F2rl2.

    Phylogenomic databases

    eggNOGi NOG146611.
    GeneTreei ENSGT00660000095329.
    HOGENOMi HOG000116291.
    HOVERGENi HBG105658.
    InParanoidi Q3UXV3.
    KOi K04235.
    OMAi CPEDSIS.
    OrthoDBi EOG7QC7WD.
    TreeFami TF330775.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Miscellaneous databases

    EvolutionaryTracei O08675.
    NextBioi 285040.
    PROi O08675.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08675.
    CleanExi MM_F2RL2.
    Genevestigatori O08675.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR003943. Prot_act_rcpt_3.
    IPR003912. Protea_act_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR01428. PROTEASEAR.
    PR01429. PROTEASEAR3.
    PROSITEi PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protease-activated receptor 3 is a second thrombin receptor in humans."
      Ishihara H., Connolly A.J., Zeng D., Kahn M.L., Zheng Y.-W., Timmons C., Tram T., Coughlin S.R.
      Nature 386:502-506(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Wolffian duct.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Asymmetric cell divisions promote stratification and differentiation of mammalian skin."
      Lechler T., Fuchs E.
      Nature 437:275-280(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH INSC AND GPSM2.
      Strain: CD-1.
      Tissue: Epidermis.

    Entry informationi

    Entry nameiPAR3_MOUSE
    AccessioniPrimary (citable) accession number: O08675
    Secondary accession number(s): B9EIT2, Q3UXV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3