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O08663

- MAP2_MOUSE

UniProt

O08663 - MAP2_MOUSE

Protein

Methionine aminopeptidase 2

Gene

Metap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation
    Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei231 – 2311SubstrateUniRule annotation
    Metal bindingi251 – 2511Divalent metal cation 1UniRule annotation
    Metal bindingi262 – 2621Divalent metal cation 1UniRule annotation
    Metal bindingi262 – 2621Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi331 – 3311Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei339 – 3391SubstrateUniRule annotation
    Metal bindingi364 – 3641Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi459 – 4591Divalent metal cation 1UniRule annotation
    Metal bindingi459 – 4591Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-HAMAP
    3. metalloaminopeptidase activity Source: UniProtKB-HAMAP
    4. metalloexopeptidase activity Source: UniProtKB
    5. protein binding Source: MGI

    GO - Biological processi

    1. N-terminal protein amino acid modification Source: HGNC
    2. peptidyl-methionine modification Source: HGNC
    3. protein initiator methionine removal Source: UniProtKB-HAMAP
    4. protein processing Source: HGNC

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.11.18. 3474.
    ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Protein family/group databases

    MEROPSiM24.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2UniRule annotation
    Short name:
    MetAP 2UniRule annotation
    Alternative name(s):
    Initiation factor 2-associated 67 kDa glycoproteinUniRule annotation
    Short name:
    p67UniRule annotation
    Short name:
    p67eIF2UniRule annotation
    Peptidase MUniRule annotation
    Gene namesi
    Name:Metap2
    Synonyms:Mnpep, P67eif2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1929701. Metap2.

    Subcellular locationi

    Cytoplasm
    Note: About 30% of expressed METAP2 associates with polysomes.UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 478477Methionine aminopeptidase 2PRO_0000148983Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei60 – 601Phosphoserine; alternateBy similarity
    Glycosylationi60 – 601O-linked (GlcNAc); alternateBy similarity

    Post-translational modificationi

    Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.UniRule annotation

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiO08663.
    PaxDbiO08663.
    PRIDEiO08663.

    PTM databases

    PhosphoSiteiO08663.

    Expressioni

    Gene expression databases

    ArrayExpressiO08663.
    BgeeiO08663.
    CleanExiMM_METAP2.
    GenevestigatoriO08663.

    Interactioni

    Subunit structurei

    Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3.UniRule annotation

    Protein-protein interaction databases

    BioGridi207891. 5 interactions.
    IntActiO08663. 1 interaction.
    MINTiMINT-1858053.

    Structurei

    3D structure databases

    ProteinModelPortaliO08663.
    SMRiO08663. Positions 110-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 466Poly-Lys
    Compositional biasi98 – 1069Poly-Lys

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0024.
    HOVERGENiHBG050495.
    InParanoidiO08663.
    KOiK01265.
    OrthoDBiEOG7QRQVF.
    PhylomeDBiO08663.
    TreeFamiTF300426.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08663-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGVEQAASF GGHLNGDLDP DDREEGTSST AEEAAKKKRR KKKKGKGAVS    50
    AVQQELDKES GALVDEVAKQ LESQALEEKE RDDDDEDGDG DADGATGKKK 100
    KKKKKKRGPK VQTDPPSVPI CDLYPNGVFP KGQECEYPPT QDGRTAAWRT 150
    TSEEKKALDQ ASEEIWNDFR EAAEAHRQVR KYVMSWIKPG MTMIEICEKL 200
    EDCSRKLIKE NGLNAGLAFP TGCSLNNCAA HYTPNAGDTT VLQYDDICKI 250
    DFGTHISGRI IDCAFTVTFN PKYDILLTAV KDATNTGIKC AGIDVRLCDV 300
    GEAIQEVMES YEVEIDGKTY QVKPIRNLNG HSIGPYRIHA GKTVPIVKGG 350
    EATRMEEGEV YAIETFGSTG KGVVHDDMEC SHYMKNFDVG HVPIRLPRTK 400
    HLLNVINENF GTLAFCRRWL DRLGESKYLM ALKNLCDLGI VDPYPPLCDI 450
    KGSYTAQFEH TILLRPTCKE VVSRGDDY 478
    Length:478
    Mass (Da):52,922
    Last modified:July 1, 1997 - v1
    Checksum:iBBB9A2AFC19952E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003144 mRNA. Translation: BAA19789.1.
    AK076804 mRNA. Translation: BAC36488.1.
    BC002213 mRNA. Translation: AAH02213.1.
    CCDSiCCDS48673.1.
    RefSeqiNP_062622.1. NM_019648.3.
    XP_006513959.1. XM_006513896.1.
    XP_006513960.1. XM_006513897.1.
    UniGeneiMm.289329.

    Genome annotation databases

    EnsembliENSMUST00000047910; ENSMUSP00000048285; ENSMUSG00000036112.
    ENSMUST00000180840; ENSMUSP00000138006; ENSMUSG00000036112.
    GeneIDi56307.
    KEGGimmu:56307.
    UCSCiuc007gve.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003144 mRNA. Translation: BAA19789.1 .
    AK076804 mRNA. Translation: BAC36488.1 .
    BC002213 mRNA. Translation: AAH02213.1 .
    CCDSi CCDS48673.1.
    RefSeqi NP_062622.1. NM_019648.3.
    XP_006513959.1. XM_006513896.1.
    XP_006513960.1. XM_006513897.1.
    UniGenei Mm.289329.

    3D structure databases

    ProteinModelPortali O08663.
    SMRi O08663. Positions 110-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207891. 5 interactions.
    IntActi O08663. 1 interaction.
    MINTi MINT-1858053.

    Chemistry

    BindingDBi O08663.
    ChEMBLi CHEMBL1075272.

    Protein family/group databases

    MEROPSi M24.002.

    PTM databases

    PhosphoSitei O08663.

    Proteomic databases

    MaxQBi O08663.
    PaxDbi O08663.
    PRIDEi O08663.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047910 ; ENSMUSP00000048285 ; ENSMUSG00000036112 .
    ENSMUST00000180840 ; ENSMUSP00000138006 ; ENSMUSG00000036112 .
    GeneIDi 56307.
    KEGGi mmu:56307.
    UCSCi uc007gve.1. mouse.

    Organism-specific databases

    CTDi 10988.
    MGIi MGI:1929701. Metap2.

    Phylogenomic databases

    eggNOGi COG0024.
    HOVERGENi HBG050495.
    InParanoidi O08663.
    KOi K01265.
    OrthoDBi EOG7QRQVF.
    PhylomeDBi O08663.
    TreeFami TF300426.

    Enzyme and pathway databases

    BRENDAi 3.4.11.18. 3474.
    Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    ChiTaRSi METAP2. mouse.
    NextBioi 312244.
    PROi O08663.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08663.
    Bgeei O08663.
    CleanExi MM_METAP2.
    Genevestigatori O08663.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sekiguchi S., Suzuki E.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 260-281; 297-318; 355-371 AND 386-395, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiMAP2_MOUSE
    AccessioniPrimary (citable) accession number: O08663
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3