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Protein

Phosphatidylinositol 4-kinase alpha

Gene

Pi4ka

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.1 Publication

Enzyme regulationi

Activated by Triton X-100, insensitive to inhibition by adenosine and inhibited by wortmannin (PubMed:8662589). The PI4K complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns4P) synthesis (By similarity). Interaction with TMEM150A regulates PtdIns4P synthesis (By similarity).By similarity1 Publication

GO - Molecular functioni

  • 1-phosphatidylinositol 4-kinase activity Source: RGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase alpha (EC:2.7.1.671 Publication)
Short name:
PI4-kinase alpha
Short name:
PI4K-alpha
Short name:
PtdIns-4-kinase alpha
Gene namesi
Name:Pi4ka
Synonyms:Pik4ca
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621213. Pi4ka.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Localization to the plasma membrane is mediated by the PI4K complex and association with EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Localization to the plasma membrane is regulated by TMEM150A.By similarity

GO - Cellular componenti

  • Golgi-associated vesicle membrane Source: RGD
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20962096Phosphatidylinositol 4-kinase alphaPRO_0000425713Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei224 – 2241PhosphoserineBy similarity
Modified residuei250 – 2501PhosphoserineBy similarity
Modified residuei251 – 2511PhosphoserineBy similarity
Modified residuei253 – 2531PhosphoserineBy similarity
Modified residuei254 – 2541PhosphoserineCombined sources
Modified residuei256 – 2561PhosphoserineBy similarity
Modified residuei259 – 2591PhosphoserineCombined sources
Modified residuei423 – 4231PhosphoserineBy similarity
Modified residuei1430 – 14301PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08662.
PRIDEiO08662.

PTM databases

iPTMnetiO08662.
PhosphoSiteiO08662.

Expressioni

Tissue specificityi

Detected in the brain, kidney and lung. Less intensely expressed in the small intestine, uterus, adrenal gland, heart, skeletal muscle, thymus, spleen and testis.1 Publication

Developmental stagei

In brain of prenatal day 18 embryos, the expression is detected throughout the mantle zone of fore-, mid-, and hind brain. In the cerebrum, the expression is intense in the cortical plate and weak in the ventricular zone. At P49, expressed in the gray matter of the entire brain by hippocampal pyramidal cells, dentate granule cells, and cerebellar granule cells and to a lower extent by olfactory mitral and granule cells and the cerebral cortex. Weakly expressed in the diencephalon and brain stem and not detected in the cerebellar medulla. Expression is much higher in the fetal brain than the adult brain, especially in the brain stem.1 Publication

Interactioni

Subunit structurei

Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Interacts with TMEM150A; regulating recruitment to the plasma membrane.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059384.

Structurei

3D structure databases

ProteinModelPortaliO08662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1524 – 1712189PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini1840 – 2073234PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0902. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000007272.
HOVERGENiHBG052742.
KOiK00888.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGARGTG GSGSSSGSST SRGFYFNTVL SLARSLAVQR PASLEKVQKL
60 70 80 90 100
LCMCPVDFHG IFQLDERRRD AVIALGIFLI ESDLQHKDCI VPYLLRLLRG
110 120 130 140 150
LPKVYWVEES TARKGRGNLP VAESFSFCLV TLLSDVACRD PSLRDEILEA
160 170 180 190 200
LLQVLHVLLG MCQALEIQEK EYLCKYAIPC LIGISRSFGR YSNSEESLLS
210 220 230 240 250
KLFPKVPPHS LRIPEELEGV RRRSFNDFRS ILPSNLLTVC QEGTLKRKTS
260 270 280 290 300
SVSSISQVSP ERGMPPPSSP GGSAFHYFEA SCLPDGTTLE PEYYFSTISS
310 320 330 340 350
SFSVSPLFNG ITYKEFYIPL EMLRELLNLV KKIVEEPVLK SLDAAVARVM
360 370 380 390 400
EANPSADLYY TTFSDPVYLT MFKMLRDTLY YMKDLPTSFV KEIHDFVLEQ
410 420 430 440 450
FNMSQGELQK ILHDADRIHS EMSPLKLRCQ ANAACVDLMV WAVKDEQGAE
460 470 480 490 500
NLCIKLSEKL QSKTSSKVII AHLPLLICCL QGLGRLCERF PVVVHSVTPS
510 520 530 540 550
LRDFLVIPSP VLVKLYKYHS QYHTVAGSDI KISVTNEHSE STLNVLPGKK
560 570 580 590 600
NQPSMYEQLR DIAIDNICRC LKAGLTVDPV IVEAFLASLS NRLYISQESD
610 620 630 640 650
KDAHLIPDHT IRALGHIAVA LRDTPKVMEP ILQILQQKFC QPPSPLDVLI
660 670 680 690 700
IDQLGCLVIT GNQYIYQEVW NLFQQISVKA SSVVYSATKD YKDHGYRHCS
710 720 730 740 750
LAVINALANI AANIQEEHLV DELLMNLLEL FVQLGLEGKR ASERASEKGP
760 770 780 790 800
ALKASSSAGN LGVLIPVIAV LTRRLPPIKE AKPRLQKLFR DFWLYSVLMG
810 820 830 840 850
FAVEGSGLWP EEWYEGVCEI ATKSPLLTFP SKEPLRSVLQ YNSAMKNDTV
860 870 880 890 900
TPAELNELRS TIINLLDPPP EVSALINKLD FAMSTYLLSV YRLEYMRVLR
910 920 930 940 950
STDPDRFQVM FCYFEDKAIQ KDKSGMMQCV IAVADKVFDA FLNMMAEKAK
960 970 980 990 1000
TKENEEELER HAQFLLVNFN HIHKRIRRVA DKYLSGLVDK FPHLLWSGTV
1010 1020 1030 1040 1050
LKTMLDILQT LSLSLSADIH KDQPYYDIPD APYRITVPDT YEARESIVKD
1060 1070 1080 1090 1100
FAARCGMILQ EAMKWAPTVT KSHLQEYLNK HQNWVSGLSQ HTGLAMATES
1110 1120 1130 1140 1150
ILHFAGYNKQ NTTLGVTQLT ERPACVKKDY SNFMASLNLR NRYAGEVHGM
1160 1170 1180 1190 1200
IRFSGATGQM SDLNKMMVQD LITALDHSHP QHYTQAMFKL TAMLISSKDC
1210 1220 1230 1240 1250
DPQLLHHLCW GPLRMFNEHG METALACWEW LLAGKNGVEV PFMREMAGAW
1260 1270 1280 1290 1300
HMTVEQKFGL FSAETKEADP LAASEASQPR PCPPEVTPHY IWIDFLVQRF
1310 1320 1330 1340 1350
EIAKYCSSDQ VEIFSSLLQR SMSLHIGGAR GSMNRHVAAI GPRFKLLTLG
1360 1370 1380 1390 1400
LSLLHADVVP NATIRNVLRE KIYSTAFDYF SCPPKFPTQG EKRLREDISI
1410 1420 1430 1440 1450
MIKFWTAMFS DKKYLTASQL VPPDNQDTRS NLDITVGSRQ QATQGWINTY
1460 1470 1480 1490 1500
PLSSGMSTIS KKSGMSKKTN RGSQLHKYYM KRRTLLLSLL ATEIERLITW
1510 1520 1530 1540 1550
YNPLSAPELE LDQAGENSVA NWRSKYISLS EKQWKDNVNL AWTISPYLAV
1560 1570 1580 1590 1600
QLPARFKNTE AIGNEVTRLV RLDPGAVSDV PEAIKFLVTW HTIDADAPEL
1610 1620 1630 1640 1650
SHVLCWAPTD PPTGLSYFSS MYPPHPLTAQ YGVKVLRSFP PDAILFYIPQ
1660 1670 1680 1690 1700
IVQALRYDKM GYVREYILWA AAKSQLLAHQ FIWNMKTNIY LDEEGHQKDP
1710 1720 1730 1740 1750
DIGDLLEQLV EEITGSLSGP AKDFYQREFD FFNKITNVSA IIKPYPKGDE
1760 1770 1780 1790 1800
RKKACLSALS EVKVQPGCYL PSNPEAIVLD IDYKSGTPMQ SAAKAPYLAK
1810 1820 1830 1840 1850
FKVKRCGVSE LEKEGLQCRS DTEDECRRQE ADGKKICWQA AIFKVGDDCR
1860 1870 1880 1890 1900
QDMLALQIID LFKNIFQLVG LDLFVFPYRV VATAPGCGVI ECIPDCTSRD
1910 1920 1930 1940 1950
QLGRQTDFGM YDYFTRQYGD ESTLAFQQAR YNFIRSMAAY SLLLFLLQIK
1960 1970 1980 1990 2000
DRHNGNIMLD KKGHIIHIDF GFMFESSPGG NLGWEPDIKL TDEMVMIMGG
2010 2020 2030 2040 2050
KMEATPFKWF MEMCVRGYLA VRPYMDAVVS LVTLMLDTGL PCFRGQTIKL
2060 2070 2080 2090
LKHRFSPNMT EREAANFIMK IIQNCFLSNR SRTYDMIQYY QNDIPY
Length:2,096
Mass (Da):236,920
Last modified:February 17, 2016 - v2
Checksum:i8CB6D0DA792F9694
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861R → K in BAA19614 (PubMed:8662589).Curated
Sequence conflicti631 – 6333Missing in BAA19614 (PubMed:8662589).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83538 mRNA. Translation: BAA19614.1.
AC111344 Genomic DNA. No translation available.
RefSeqiNP_071637.1. NM_022301.1.
UniGeneiRn.11015.

Genome annotation databases

GeneIDi64161.
KEGGirno:64161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83538 mRNA. Translation: BAA19614.1.
AC111344 Genomic DNA. No translation available.
RefSeqiNP_071637.1. NM_022301.1.
UniGeneiRn.11015.

3D structure databases

ProteinModelPortaliO08662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059384.

PTM databases

iPTMnetiO08662.
PhosphoSiteiO08662.

Proteomic databases

PaxDbiO08662.
PRIDEiO08662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64161.
KEGGirno:64161.

Organism-specific databases

CTDi5297.
RGDi621213. Pi4ka.

Phylogenomic databases

eggNOGiKOG0902. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000007272.
HOVERGENiHBG052742.
KOiK00888.

Miscellaneous databases

PROiO08662.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI4KA_RAT
AccessioniPrimary (citable) accession number: O08662
Secondary accession number(s): A0A0G2K2J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: February 17, 2016
Last modified: July 6, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.