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O08651 (SERA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-3-phosphoglycerate dehydrogenase
Short name=3-PGDH
EC=1.1.1.95
Gene names
Name:Phgdh
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH.

2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.

Pathway

Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.

Subunit structure

Homotetramer.

Tissue specificity

Liver, kidney, brain, testis.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 533532D-3-phosphoglycerate dehydrogenase
PRO_0000076016

Regions

Nucleotide binding155 – 1562NAD By similarity
Nucleotide binding234 – 2363NAD By similarity
Nucleotide binding283 – 2864NAD By similarity

Sites

Active site2361 By similarity
Active site2651 By similarity
Active site2831Proton donor By similarity
Binding site781NAD By similarity
Binding site1751NAD By similarity
Binding site2071NAD; via carbonyl oxygen By similarity
Binding site2601NAD By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3711Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
O08651 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7273DAC3349E95EF

FASTA53356,493
        10         20         30         40         50         60 
MAFANLRKIL ISDSLDPCCR KILQDGGLQV VEKQNLSKEE LIAELQDCEG LIVRSATKVT 

        70         80         90        100        110        120 
ADVINAAEKL QVVGRAGTGV DNVDLEAATR KGVLVMNTPN GNSLSAAELT CGMLMCLARQ 

       130        140        150        160        170        180 
IPQATASMKD GKWDRKKFMG TELNGKTLGI LGLGRIGREV AARMQAFGMK TVGYDPIISP 

       190        200        210        220        230        240 
EVAASFGVQQ LPLEEIWPLC DFITVHTPLL PSTTGLLNDS TFAQCKKGVR VVNCARGGIV 

       250        260        270        280        290        300 
DEGALLRALQ SGQCAGAALD VFTEEPPRDR ALVDHENVIS CPHLGASTKE AQSRCGEEIA 

       310        320        330        340        350        360 
VQFVDMVKGK SLTGVVNAQA LTSAFSPHTK PWIGLAEALG TLMHAWAGSP KGTIQVVTQG 

       370        380        390        400        410        420 
TSLKNAGTCL SPAVIVGLLR EASKQADVNL VNAKLLVKEA GLNVTTSHSP GVPGEQGIGE 

       430        440        450        460        470        480 
CLLTVALAGA PYQAVGLVQG TTPMLQMLNG AVFRPEVPLR RGQPLLLFRA QPSDPVMLPT 

       490        500        510        520        530 
MIGLLAEAGV QLLSYQTSKV SDGDTWHVMG LSSLLPSLDA WKQHVSEAFQ FCF

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and expression of rat liver 3-phosphoglycerate dehydrogenase."
Achouri Y., Rider M.H., van Schaftingen E., Robbi M.
Biochem. J. 323:365-370(1997) [PubMed: 9163325] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and chromosomal localization of the rat gene encoding 3-phosphoglycerate dehydrogenase."
Robbi M., Achouri Y., Szpirer C., Van Schaftingen E.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]Lubec G., Afjehi-Sadat L.
Submitted (NOV-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 34-54; 248-270; 271-289 AND 365-380, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X97772 mRNA. Translation: CAA66374.1.
AJ271975 Genomic DNA. Translation: CAB89828.1.
BC086327 mRNA. Translation: AAH86327.1.
IPIIPI00475835.
RefSeqNP_113808.1. NM_031620.1.
UniGeneRn.6872.

3D structure databases

ProteinModelPortalO08651.
SMRO08651. Positions 6-307.
ModBaseSearch...

Protein-protein interaction databases

IntActO08651. 1 interaction.
STRINGO08651.

2D gel databases

World-2DPAGE0004:O08651.

Proteomic databases

PRIDEO08651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000056173; ENSRNOP00000053019; ENSRNOG00000019328.
GeneID58835.
KEGGrno:58835.
NMPDRfig|10116.3.peg.16688.
UCSCBC086327. rat.

Organism-specific databases

CTD26227.
RGD61987. Phgdh.

Phylogenomic databases

eggNOGroNOG06496.
GeneTreeENSGT00530000063021.
HOVERGENHBG054241.
InParanoidO08651.
OMATGVFDGY.
OrthoDBEOG4Q2DF9.
PhylomeDBO08651.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-10261.

Gene expression databases

ArrayExpressO08651.
GenevestigatorO08651.
GermOnlineENSRNOG00000019328. Rattus norvegicus.

Family and domain databases

InterProIPR006236. D-3-Phosphoglycerate_DH.
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK00058.
PANTHERPTHR10996:SF20. D3PG_Deh. 1 hit.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01327. PGDH. 1 hit.
PROSITEPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611397.

Entry information

Entry nameSERA_RAT
AccessionPrimary (citable) accession number: O08651
Secondary accession number(s): Q546Q9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 16, 2011
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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