Reviewed, UniProtKB/Swiss-Prot O08651 (SERA_RAT)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

Name:

Phgdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Subunit structure	Homotetramer.
Tissue specificity	Liver, kidney, brain, testis.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 533	532	D-3-phosphoglycerate dehydrogenase		PRO_0000076016
Sites
	Active site	236	1	By similarity
	Active site	265	1	By similarity
	Active site	283	1	Proton donor By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	371	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08651-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7273DAC3349E95EF	FASTA	533	56,493
	10 20 30 40 50 60 MAFANLRKIL ISDSLDPCCR KILQDGGLQV VEKQNLSKEE LIAELQDCEG LIVRSATKVT 70 80 90 100 110 120 ADVINAAEKL QVVGRAGTGV DNVDLEAATR KGVLVMNTPN GNSLSAAELT CGMLMCLARQ 130 140 150 160 170 180 IPQATASMKD GKWDRKKFMG TELNGKTLGI LGLGRIGREV AARMQAFGMK TVGYDPIISP 190 200 210 220 230 240 EVAASFGVQQ LPLEEIWPLC DFITVHTPLL PSTTGLLNDS TFAQCKKGVR VVNCARGGIV 250 260 270 280 290 300 DEGALLRALQ SGQCAGAALD VFTEEPPRDR ALVDHENVIS CPHLGASTKE AQSRCGEEIA 310 320 330 340 350 360 VQFVDMVKGK SLTGVVNAQA LTSAFSPHTK PWIGLAEALG TLMHAWAGSP KGTIQVVTQG 370 380 390 400 410 420 TSLKNAGTCL SPAVIVGLLR EASKQADVNL VNAKLLVKEA GLNVTTSHSP GVPGEQGIGE 430 440 450 460 470 480 CLLTVALAGA PYQAVGLVQG TTPMLQMLNG AVFRPEVPLR RGQPLLLFRA QPSDPVMLPT 490 500 510 520 530 MIGLLAEAGV QLLSYQTSKV SDGDTWHVMG LSSLLPSLDA WKQHVSEAFQ FCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and expression of rat liver 3-phosphoglycerate dehydrogenase." Achouri Y., Rider M.H., van Schaftingen E., Robbi M. Biochem. J. 323:365-370(1997) [PubMed: 9163325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure and chromosomal localization of the rat gene encoding 3-phosphoglycerate dehydrogenase." Robbi M., Achouri Y., Szpirer C., Van Schaftingen E. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 34-54; 248-270; 271-289 AND 365-380, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97772 mRNA. Translation: CAA66374.1. AJ271975 Genomic DNA. Translation: CAB89828.1. BC086327 mRNA. Translation: AAH86327.1.
IPI	IPI00475835.
RefSeq	NP_113808.1.
UniGene	Rn.6872
3D structure databases
HSSP	HSSP built from PDB template 1HL3 based on UniProtKB Q9Z2F5.
SMR	O08651. Positions 5-306, 6-307.
ModBase	Search...
Proteomic databases
PRIDE	O08651.
Genome annotation databases
Ensembl	ENSRNOG00000019328. Rattus norvegicus. [Contig view]
GeneID	58835.
KEGG	rno:58835.
NMPDR	fig|10116.3.peg.16688.
Organism-specific databases
RGD	61987. Phgdh.
Phylogenomic databases
HOVERGEN	O08651.
OMA	O08651. VEQMPLE.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-10261.
BRENDA	1.1.1.95. 248.
Gene expression databases
ArrayExpress	O08651.
GermOnline	ENSRNOG00000019328. Rattus norvegicus.
Family and domain databases
InterPro	IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR015508. D3PG_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611397.

Entry information

Entry name

SERA_RAT

Accession

Primary (citable) accession number: O08651
Secondary accession number(s): Q546Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)