Reviewed, UniProtKB/Swiss-Prot O08650 (HAS3_MOUSE)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase 3
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase 3
    Hyaluronic acid synthase 3
      Short name=HA synthase 3

Gene names

Name:

Has3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Developmental stage	Expressed at E17.5 day.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Hyaluronan synthase 3		PRO_0000197179
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	1 Potential
	Topological domain	37 – 44	8	Extracellular Potential
	Transmembrane	45 – 65	21	2 Potential
	Topological domain	66 – 378	313	Cytoplasmic Potential
	Transmembrane	379 – 399	21	3 Potential
	Topological domain	400 – 409	10	Extracellular Potential
	Transmembrane	410 – 430	21	4 Potential
	Topological domain	431 – 441	11	Cytoplasmic Potential
	Transmembrane	442 – 462	21	5 Potential
	Topological domain	463 – 474	12	Extracellular Potential
	Transmembrane	475 – 495	21	6 Potential
	Topological domain	496 – 516	21	Cytoplasmic Potential
	Transmembrane	517 – 537	21	7 Potential
	Topological domain	538 – 554	17	Extracellular Potential
Amino acid modifications
	Glycosylation	463	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O08650-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 88296DCB465CE3EC	FASTA	554	63,339
	10 20 30 40 50 60 MPVQLTTALR VVGTSLFALV VLGGILAAYV TGYQFIHTEK HYLSFGLYGA ILGLHLLIQS 70 80 90 100 110 120 LFAFLEHRRM RRAGRPLKLH CSQRSRSVAL CIAAYQEDPE YLRKCLRSAQ RIAFPNLKVV 130 140 150 160 170 180 MVVDGNRQED TYMLDIFHEV LGGTEQAGFF VWRSNFHEAG EGETEASLQE GMERVRAVVW 190 200 210 220 230 240 ASTFSCIMQK WGGKREVMYT AFKALGNSVD YIQVCDSDTV LDPACTIEML RVLEEDPQVG 250 260 270 280 290 300 GVGGDVQILN KYDSWISFLS SVRYWMAFNV ERACQSYFGC VQCISGPLGM YRNSLLQQFL 310 320 330 340 350 360 EDWYHQKFLG SKCSFGDDRH LTNRVLSLGY RTKYTARSKC LTETPTRYLR WLNQQTRWSK 370 380 390 400 410 420 SYFREWLYNS LWFHKHHLWM TYESVVTGFF PFFLIATVIQ LFYRGRIWNI LLFLLTVQLV 430 440 450 460 470 480 GIIKATYACF LRGNAEMIFM SLYSLLYMSS LLPAKIFAIA TINKSGWGTS GRKTIVVNFI 490 500 510 520 530 540 GLIPVSIWVA VLLGGLAYTA YCQDLFSETE LAFLVSGAIL YGCYWVALLM LYLAIIARRC 550 GKKPEQYSLA FAEV

References

[1]	"Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase." Spicer A.P., Olson J.S., McDonald J.A. J. Biol. Chem. 272:8957-8961(1997) [PubMed: 9083017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvJ and C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U86408 mRNA. Translation: AAC53128.1.
IPI	IPI00114919.
RefSeq	XP_001479297.1. XP_001479305.1.
UniGene	Mm.56986
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O08650.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PRIDE	O08650.
Genome annotation databases
Ensembl	ENSMUST00000034385; ENSMUSP00000034385; ENSMUSG00000031910; Mus musculus. [Genome view]
GeneID	100047776.
KEGG	mmu:100047776.
UCSC	uc009ngm.1. mouse.
Organism-specific databases
MGI	MGI:109599. Has3.
Phylogenomic databases
HOGENOM	O08650.
HOVERGEN	O08650.
Enzyme and pathway databases
BRENDA	2.4.1.212. 244.
Gene expression databases
ArrayExpress	O08650.
Bgee	O08650.
CleanEx	MM_HAS3.
Genevestigator	O08650.
GermOnline	ENSMUSG00000031910. Mus musculus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	461202.
SOURCE	Search...

Entry information

Entry name

HAS3_MOUSE

Accession

Primary (citable) accession number: O08650

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)