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Protein

Hyaluronan synthase 3

Gene

Has3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction.1 Publication

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

Cofactori

Kineticsi

  1. KM=0.2 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication
  2. KM=0.3 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication

    Pathway:ihyaluronan biosynthesis

    This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

    GO - Molecular functioni

    • hyaluronan synthase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.212. 3474.
    ReactomeiREACT_299251. Hyaluronan biosynthesis and export.
    UniPathwayiUPA00341.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronan synthase 3 (EC:2.4.1.212)
    Alternative name(s):
    Hyaluronate synthase 3
    Hyaluronic acid synthase 3
    Short name:
    HA synthase 3
    Gene namesi
    Name:Has3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:109599. Has3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei16 – 3621Helical; Name=1Sequence AnalysisAdd
    BLAST
    Topological domaini37 – 448ExtracellularSequence Analysis
    Transmembranei45 – 6521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Topological domaini66 – 378313CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39921Helical; Name=3Sequence AnalysisAdd
    BLAST
    Topological domaini400 – 40910ExtracellularSequence Analysis
    Transmembranei410 – 43021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Topological domaini431 – 44111CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Topological domaini463 – 47412ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei475 – 49521Helical; Name=6Sequence AnalysisAdd
    BLAST
    Topological domaini496 – 51621CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei517 – 53721Helical; Name=7Sequence AnalysisAdd
    BLAST
    Topological domaini538 – 55417ExtracellularSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • cytoplasm Source: CACAO
    • hyaluranon cable Source: MGI
    • integral component of plasma membrane Source: GO_Central
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Hyaluronan synthase 3PRO_0000197179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi463 – 4631N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiO08650.

    PTM databases

    PhosphoSiteiO08650.

    Expressioni

    Developmental stagei

    Expressed at E17.5 day.

    Gene expression databases

    BgeeiO08650.
    CleanExiMM_HAS3.
    ExpressionAtlasiO08650. baseline and differential.
    GenevisibleiO08650. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034385.

    Structurei

    3D structure databases

    ProteinModelPortaliO08650.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NodC/HAS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1215.
    GeneTreeiENSGT00390000010337.
    HOGENOMiHOG000112847.
    HOVERGENiHBG000189.
    InParanoidiO08650.
    KOiK00752.
    OMAiRGRLWNI.
    OrthoDBiEOG77WWBZ.
    TreeFamiTF332506.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR026108. HA_synthase_3.
    IPR026107. HAS/NodC.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF6. PTHR22913:SF6. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O08650-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPVQLTTALR VVGTSLFALV VLGGILAAYV TGYQFIHTEK HYLSFGLYGA
    60 70 80 90 100
    ILGLHLLIQS LFAFLEHRRM RRAGRPLKLH CSQRPRSVAL CIAAYQEDPE
    110 120 130 140 150
    YLRKCLRSAQ RIAFPNLKVV MVVDGNRQED TYMLDIFHEV LGGTEQAGFF
    160 170 180 190 200
    VWRSNFHEAG EGETEASLQE GMERVRAVVW ASTFSCIMQK WGGKREVMYT
    210 220 230 240 250
    AFKALGNSVD YIQVCDSDTV LDPACTIEML RVLEEDPQVG GVGGDVQILN
    260 270 280 290 300
    KYDSWISFLS SVRYWMAFNV ERACQSYFGC VQCISGPLGM YRNSLLQQFL
    310 320 330 340 350
    EDWYHQKFLG SKCSFGDDRH LTNRVLSLGY RTKYTARSKC LTETPTRYLR
    360 370 380 390 400
    WLNQQTRWSK SYFREWLYNS LWFHKHHLWM TYESVVTGFF PFFLIATVIQ
    410 420 430 440 450
    LFYRGRIWNI LLFLLTVQLV GIIKATYACF LRGNAEMIFM SLYSLLYMSS
    460 470 480 490 500
    LLPAKIFAIA TINKSGWGTS GRKTIVVNFI GLIPVSIWVA VLLGGLAYTA
    510 520 530 540 550
    YCQDLFSETE LAFLVSGAIL YGCYWVALLM LYLAIIARRC GKKPEQYSLA

    FAEV
    Length:554
    Mass (Da):63,349
    Last modified:July 27, 2011 - v2
    Checksum:iBFFF239C901251DE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851P → S in AAC53128 (PubMed:9083017).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U86408 mRNA. Translation: AAC53128.1.
    AK028582 mRNA. Translation: BAC26017.1.
    BC138152 mRNA. Translation: AAI38153.1.
    CCDSiCCDS22640.1.
    RefSeqiNP_032243.2. NM_008217.4.
    XP_006530762.1. XM_006530699.1.
    XP_006530763.1. XM_006530700.2.
    XP_006530764.1. XM_006530701.1.
    UniGeneiMm.56986.

    Genome annotation databases

    EnsembliENSMUST00000034385; ENSMUSP00000034385; ENSMUSG00000031910.
    ENSMUST00000176144; ENSMUSP00000135303; ENSMUSG00000031910.
    GeneIDi15118.
    KEGGimmu:15118.
    UCSCiuc009ngn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U86408 mRNA. Translation: AAC53128.1.
    AK028582 mRNA. Translation: BAC26017.1.
    BC138152 mRNA. Translation: AAI38153.1.
    CCDSiCCDS22640.1.
    RefSeqiNP_032243.2. NM_008217.4.
    XP_006530762.1. XM_006530699.1.
    XP_006530763.1. XM_006530700.2.
    XP_006530764.1. XM_006530701.1.
    UniGeneiMm.56986.

    3D structure databases

    ProteinModelPortaliO08650.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034385.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    PTM databases

    PhosphoSiteiO08650.

    Proteomic databases

    PRIDEiO08650.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000034385; ENSMUSP00000034385; ENSMUSG00000031910.
    ENSMUST00000176144; ENSMUSP00000135303; ENSMUSG00000031910.
    GeneIDi15118.
    KEGGimmu:15118.
    UCSCiuc009ngn.2. mouse.

    Organism-specific databases

    CTDi3038.
    MGIiMGI:109599. Has3.

    Phylogenomic databases

    eggNOGiCOG1215.
    GeneTreeiENSGT00390000010337.
    HOGENOMiHOG000112847.
    HOVERGENiHBG000189.
    InParanoidiO08650.
    KOiK00752.
    OMAiRGRLWNI.
    OrthoDBiEOG77WWBZ.
    TreeFamiTF332506.

    Enzyme and pathway databases

    UniPathwayiUPA00341.
    BRENDAi2.4.1.212. 3474.
    ReactomeiREACT_299251. Hyaluronan biosynthesis and export.

    Miscellaneous databases

    NextBioi287538.
    PROiO08650.
    SOURCEiSearch...

    Gene expression databases

    BgeeiO08650.
    CleanExiMM_HAS3.
    ExpressionAtlasiO08650. baseline and differential.
    GenevisibleiO08650. MM.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR026108. HA_synthase_3.
    IPR026107. HAS/NodC.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF6. PTHR22913:SF6. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase."
      Spicer A.P., Olson J.S., McDonald J.A.
      J. Biol. Chem. 272:8957-8961(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/SvJ and C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, KINETIC PARAMETERS.

    Entry informationi

    Entry nameiHYAS3_MOUSE
    AccessioniPrimary (citable) accession number: O08650
    Secondary accession number(s): Q8CEB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: June 24, 2015
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.