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O08644

- EPHB6_MOUSE

UniProt

O08644 - EPHB6_MOUSE

Protein

Ephrin type-B receptor 6

Gene

Ephb6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 4 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi669 – 6779ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ephrin receptor activity Source: InterPro

    GO - Biological processi

    1. calcium ion homeostasis Source: MGI
    2. parathyroid hormone secretion Source: MGI

    Keywords - Molecular functioni

    Receptor

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin type-B receptor 6
    Alternative name(s):
    MEP
    Tyrosine-protein kinase-defective receptor EPH-6
    Gene namesi
    Name:Ephb6
    Synonyms:Cekl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1096338. Ephb6.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232By similarityAdd
    BLAST
    Chaini33 – 1014982Ephrin type-B receptor 6PRO_0000016838Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO08644.
    PRIDEiO08644.

    PTM databases

    PhosphoSiteiO08644.

    Expressioni

    Tissue specificityi

    High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells.1 Publication

    Gene expression databases

    BgeeiO08644.
    CleanExiMM_EPHB6.
    GenevestigatoriO08644.

    Interactioni

    Subunit structurei

    Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO08644.
    SMRiO08644. Positions 32-580, 640-919, 949-1000.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 591559ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini613 – 1014402CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei592 – 61221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 232199Eph LBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini364 – 479116Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini480 – 57596Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini663 – 912250Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini941 – 100565SAMPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1012 – 10143PDZ-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi168 – 1736Poly-Ser
    Compositional biasi214 – 361148Cys-richAdd
    BLAST
    Compositional biasi874 – 8774Poly-Pro

    Domaini

    The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2 By similarity.PROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
    Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
    Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115081.
    HOGENOMiHOG000233856.
    HOVERGENiHBG062180.
    KOiK05114.
    OMAiEQAVAIQ.
    OrthoDBiEOG7VTDM6.
    PhylomeDBiO08644.
    TreeFamiTF314013.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 2 hits.
    InterProiIPR027936. Eph_TM.
    IPR001090. Ephrin_rcpt_lig-bd_dom.
    IPR003961. Fibronectin_type3.
    IPR008979. Galactose-bd-like.
    IPR013783. Ig-like_fold.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
    IPR016257. Tyr_kinase_ephrin_rcpt.
    IPR001426. Tyr_kinase_rcpt_V_CS.
    [Graphical view]
    PfamiPF14575. EphA2_TM. 1 hit.
    PF01404. Ephrin_lbd. 1 hit.
    PF00041. fn3. 2 hits.
    PF07699. GCC2_GCC3. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07647. SAM_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00615. EPH_lbd. 1 hit.
    SM00060. FN3. 2 hits.
    SM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF49265. SSF49265. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51550. EPH_LBD. 1 hit.
    PS50853. FN3. 2 hits.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
    PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: O08644-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATEGTTGSG SRVVAGMVCS LWLLVLGSSV LALEEVLLDT TGETSEIGWL     50
    TYPPGGWDEV SVLDDQRRLT RTFEACHVAG LPPGSGQDNW LQTHFVERRG 100
    AQRAHIRLHF SVRACSSLGV SGGTCRETFT LYYRQADEPD GPDSIAAWHL 150
    KRWTKVDTIA ADESFPASSS SSSWAVGPHR TGQRVGLQLN VKERSFGPLT 200
    QRGFYVAFQD TGACLALVAV KLFSYTCPSV LRAFASFPET QASGAGGASL 250
    VAAVGTCVAH AEPEEDGVGG QAGGSPPRLH CNGEGRWMVA VGGCRCQPGH 300
    QPARGDKLCQ ACPEGSYKAL AGNVPCSPCP ARSHSPDPAA PVCPCLQGFY 350
    RASSDPPEAP CTGPPSAPRE LWFEVQGSAL MLHWRLPQEL GGRGDLLFNV 400
    VCKECGGHGE PSSGGMCRRC RDEVHFDPRQ RGLTESRVLV GGLRAHVPYI 450
    LEVQAVNGVS ELSPDPPQAA AINVSTSHEV PSAVPVMHQV SRAANSITVS 500
    WPQPEQTNGN ILDYQLRYYD QAEDESHSFT MTSETNTATV TRLSPGHIYG 550
    FQVRARTAAG HGPYGGKVYF QTLPQGELSS QLPEKLSLVI GSILGALAFL 600
    LLAAITVLAV IFQRKRRGTG YTEQLQQYSS PGLGVKYYID PSTYDDPCQA 650
    IRELAREVDP TYIKIEEVIG AGSFGEVRRG RLQPRGRREQ AVAIQALWAG 700
    GAESLKMTFL GRAALLGQFQ HPNILRLEGV VTKSRPVMVL TELMELGPLD 750
    SFLRQREGQF SSLQLVAMQR GVAAAMQYLS SFAFVHRALS ARSVLVNSHL 800
    VCKVARLGHS PQGSSSLLRW AAPEVITHGK YTTSSDVWSF GILMWEVMSY 850
    GERPYWDMNE QEVLNAIEQE FRLPPPPGCP PGLHLLMLDT WQKDRARRPH 900
    FDQLVAAFDK MIRKPDTLQA EGGSGDRPSQ ALLNPVALDF PCLDSPQAWL 950
    SAIGLECYQD NFSKFGLSTF SDVAQLSLED LPGLGITLAG HQKKLLHNIQ 1000
    LLQQHLRQPG SVEV 1014
    Length:1,014
    Mass (Da):110,107
    Last modified:April 20, 2010 - v4
    Checksum:i1091796E1F68A842
    GO
    Isoform 2 (identifier: O08644-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         328-328: P → PCPALPLFTEHSRPEVC
         329-1014: Missing.

    Show »
    Length:344
    Mass (Da):36,465
    Checksum:i58A33AC4FD2DC032
    GO
    Isoform 3 (identifier: O08644-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         583-583: P → S
         584-1014: Missing.

    Show »
    Length:583
    Mass (Da):62,298
    Checksum:i08E5C159BE0F376A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti527 – 5271H → Y in BAC39868. (PubMed:16141072)Curated
    Sequence conflicti544 – 5441S → T in AAH31924. (PubMed:15489334)Curated
    Sequence conflicti732 – 7321T → A in AAB51430. (PubMed:8761299)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei328 – 3281P → PCPALPLFTEHSRPEVC in isoform 2. 1 PublicationVSP_003020
    Alternative sequencei329 – 1014686Missing in isoform 2. 1 PublicationVSP_003021Add
    BLAST
    Alternative sequencei583 – 5831P → S in isoform 3. 1 PublicationVSP_003022
    Alternative sequencei584 – 1014431Missing in isoform 3. 1 PublicationVSP_003023Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77867 mRNA. Translation: AAB51430.1.
    AK087423 mRNA. Translation: BAC39868.1.
    AK163823 mRNA. Translation: BAE37507.1.
    BC031924 mRNA. Translation: AAH31924.1.
    CCDSiCCDS20051.1. [O08644-1]
    RefSeqiNP_001139823.1. NM_001146351.1. [O08644-1]
    NP_031706.3. NM_007680.4. [O08644-1]
    UniGeneiMm.271976.

    Genome annotation databases

    EnsembliENSMUST00000114732; ENSMUSP00000110380; ENSMUSG00000029869. [O08644-1]
    GeneIDi13848.
    KEGGimmu:13848.
    UCSCiuc009bqa.2. mouse. [O08644-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77867 mRNA. Translation: AAB51430.1 .
    AK087423 mRNA. Translation: BAC39868.1 .
    AK163823 mRNA. Translation: BAE37507.1 .
    BC031924 mRNA. Translation: AAH31924.1 .
    CCDSi CCDS20051.1. [O08644-1 ]
    RefSeqi NP_001139823.1. NM_001146351.1. [O08644-1 ]
    NP_031706.3. NM_007680.4. [O08644-1 ]
    UniGenei Mm.271976.

    3D structure databases

    ProteinModelPortali O08644.
    SMRi O08644. Positions 32-580, 640-919, 949-1000.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O08644.

    Proteomic databases

    PaxDbi O08644.
    PRIDEi O08644.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000114732 ; ENSMUSP00000110380 ; ENSMUSG00000029869 . [O08644-1 ]
    GeneIDi 13848.
    KEGGi mmu:13848.
    UCSCi uc009bqa.2. mouse. [O08644-1 ]

    Organism-specific databases

    CTDi 2051.
    MGIi MGI:1096338. Ephb6.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115081.
    HOGENOMi HOG000233856.
    HOVERGENi HBG062180.
    KOi K05114.
    OMAi EQAVAIQ.
    OrthoDBi EOG7VTDM6.
    PhylomeDBi O08644.
    TreeFami TF314013.

    Miscellaneous databases

    ChiTaRSi EPHB6. mouse.
    NextBioi 284696.
    PROi O08644.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08644.
    CleanExi MM_EPHB6.
    Genevestigatori O08644.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 2 hits.
    InterProi IPR027936. Eph_TM.
    IPR001090. Ephrin_rcpt_lig-bd_dom.
    IPR003961. Fibronectin_type3.
    IPR008979. Galactose-bd-like.
    IPR013783. Ig-like_fold.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
    IPR016257. Tyr_kinase_ephrin_rcpt.
    IPR001426. Tyr_kinase_rcpt_V_CS.
    [Graphical view ]
    Pfami PF14575. EphA2_TM. 1 hit.
    PF01404. Ephrin_lbd. 1 hit.
    PF00041. fn3. 2 hits.
    PF07699. GCC2_GCC3. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07647. SAM_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00615. EPH_lbd. 1 hit.
    SM00060. FN3. 2 hits.
    SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF49265. SSF49265. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51550. EPH_LBD. 1 hit.
    PS50853. FN3. 2 hits.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
    PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new member of the Eph family of receptors that lacks protein tyrosine kinase activity."
      Gurniak C.B., Berg L.J.
      Oncogene 13:777-786(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
      Strain: BALB/C X 129.
      Tissue: Thymus.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Eye and Head.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiEPHB6_MOUSE
    AccessioniPrimary (citable) accession number: O08644
    Secondary accession number(s): Q3TQ77, Q8BN76, Q8K0A9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 147 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3