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O08638 (MYH11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-11
Alternative name(s):
Myosin heavy chain 11
Myosin heavy chain, smooth muscle isoform
SMMHC
Gene names
Name:Myh11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1972 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Melanosome By similarity. Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Thick filament
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandATP-binding
Actin-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionMotor protein
Muscle protein
Myosin
   PTMMethylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcardiac muscle fiber development

Inferred from sequence or structural similarity. Source: UniProtKB

elastic fiber assembly

Inferred from sequence or structural similarity. Source: UniProtKB

skeletal muscle myosin thick filament assembly

Inferred from sequence or structural similarity. Source: UniProtKB

smooth muscle contraction

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentmelanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

muscle myosin complex

Inferred from direct assay PubMed 10854329PubMed 11715025. Source: MGI

myofibril

Inferred from electronic annotation. Source: UniProtKB-SubCell

myosin filament

Inferred from electronic annotation. Source: UniProtKB-KW

smooth muscle contractile fiber

Inferred from direct assay PubMed 11715025. Source: MGI

stress fiber

Inferred from direct assay Ref.3. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

motor activity

Inferred from mutant phenotype PubMed 10854329. Source: MGI

structural constituent of muscle

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O08638-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O08638-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1930-1972: RGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE → GPPPQETSQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19721972Myosin-11
PRO_0000123425

Regions

Domain1 – 785785Myosin head-like
Domain786 – 81530IQ
Nucleotide binding178 – 1858ATP Potential
Region661 – 68323Actin-binding By similarity
Region762 – 77615Actin-binding By similarity
Region1935 – 197238C-terminal
Coiled coil844 – 19341091 Potential

Amino acid modifications

Modified residue1291N6,N6,N6-trimethyllysine Potential
Modified residue19541Phosphoserine Ref.5

Natural variations

Alternative sequence1930 – 197243RGNEA…TKASE → GPPPQETSQ in isoform 2.
VSP_003346

Experimental info

Sequence conflict1261N → D in AAB36168. Ref.3
Sequence conflict1611A → V in AAA67552. Ref.2
Sequence conflict1891Q → K in AAA67552. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: A1398E3F5B11F15A

FASTA1,972227,028
        10         20         30         40         50         60 
MAQKGQLSDD EKFLFVDKNF MNSPMAQADW VAKKLVWVPS EKQGFEAASI KEEKGDEVVV 

        70         80         90        100        110        120 
ELVENGKKVT VGKDDIQKMN PPKFSKVEDM AELTCLNEAS VLHNLRERYF SGLIYTYSGL 

       130        140        150        160        170        180 
FCVVVNPYKY LPIYSEKIVD MYKGKKRHEM PPHIYAIADT AYRSMLQDRE DQSILCTGES 

       190        200        210        220        230        240 
GAGKTENTQK VIQYLAVVAS SHKGKKDSSI TGELEKQLLQ ANPILEAFGN AKTVKNDNSS 

       250        260        270        280        290        300 
RFGKFIRINF DVTGYIVGAN IETYLLEKSR AIRQARDERT FHIFYYLLAG AKEKMKSDLL 

       310        320        330        340        350        360 
LESFNSYTFL SNGFVPIPAA QDDEMFQETL EAMSIMGFNE EEQLAILKVV SSVLQLGNIV 

       370        380        390        400        410        420 
FKKERNTDQA SMPDNTAAQK VCHLVGINVT DFTRAILTPR IKVGRDVVQK AQTKEQADFA 

       430        440        450        460        470        480 
IEALAKATYE RLFRWILSRV NKALDKTHRQ GASFLGILDI AGFEIFEVNS FEQLCINYTN 

       490        500        510        520        530        540 
EKLQQLFNHT MFILEQEEYQ REGIEWNFID FGLDLQPSIE LIERPNNPPG VLALLDEECW 

       550        560        570        580        590        600 
FPKATDKSFV EKLCSEQGNH PKFQKPKQLK DKTEFSIIHY AGKVDYNASA WLTKNMDPLN 

       610        620        630        640        650        660 
DNVTSLLNAS SDKFVADLWK DVDRIVGLDQ MAKMTESSLP SASKTKKGMF RTVGQLYKEQ 

       670        680        690        700        710        720 
LGKLMATLRN TTANFVRCII PNHEKRSGKL DAFLVLEQLR CNGVLEGIRI CRQGFPNRIV 

       730        740        750        760        770        780 
FQEFRQRYEI LAANAIPKGF MDGKQACILM IKALELDPNL YRIGQSKIFF RTGVLAHLEE 

       790        800        810        820        830        840 
ERDLKITDVI MAFQAMCRGY LARKAFTKRQ QQLTAMKVIQ RNCAAYLKLR NWQWWRLFTK 

       850        860        870        880        890        900 
VKPLLQVTRQ EEEMQAKEEE MQKITERQQK AETELKELEQ KHTQLAEEKT LLQEQLQAET 

       910        920        930        940        950        960 
ELYAESEEMR VRLAAKKQEL EEILHEMEAR LEEEEDRRQQ LQAERKKMAQ QMLDLEEQLE 

       970        980        990       1000       1010       1020 
EEEAARQKLQ LEKVTAEAKI KKLEDDILVM DDQNSKLSKE RKLLEERVSD LTTNLAEEEE 

      1030       1040       1050       1060       1070       1080 
KAKNLTKLKS KHESMISELE VRLKKEEKSR QELEKLKRKL EGDASDFHEQ IADLQAQIAE 

      1090       1100       1110       1120       1130       1140 
LKMQLAKKEE ELQAALARLD EEIAQKNNAL KKIRELEGHI SDLQEDLDSE RAARNKAEKQ 

      1150       1160       1170       1180       1190       1200 
KRDLGEELEA LKTELEDTLD STATQQELRA KREQEVTVLK KALDEETRSH EAQVQEMRQK 

      1210       1220       1230       1240       1250       1260 
HTQAVEELTE QLEQFKRAKA NLDKSKQTLE KENADLAGEL RVLGQAKQEV EHKKKKLEVQ 

      1270       1280       1290       1300       1310       1320 
LQDLQSKCSD GERARAELSD KVHKLQNEVE SVTGMLNEAE GKAIKLAKDV ASLGSQLQDT 

      1330       1340       1350       1360       1370       1380 
QELLQEETRQ KLNVSTKLRQ LEDERNSLQD QLDEEMEAKQ NLERHVSTLN IQLSDSKKKL 

      1390       1400       1410       1420       1430       1440 
QDFASTIEVM EEGKKRLQKE MEGLSQQYEE KAAAYDKLEK TKNRLQQELD DLVVDLDNQR 

      1450       1460       1470       1480       1490       1500 
QLVSNLEKKQ KKFDQLLAEE KNISSKYADE RDRAEAEARE KETKALSLAR ALEEALEAKE 

      1510       1520       1530       1540       1550       1560 
ELERTNKMLK AEMEDLVSSK DDVGKNVHEL EKSKRALETQ MEEMKTQLEE SEDDVQATED 

      1570       1580       1590       1600       1610       1620 
AKLRLEVNMQ ALKGQFERDL QARDEQNEEK RRQLQRQLHE YETELEDERK QRALAAAAKK 

      1630       1640       1650       1660       1670       1680 
KLEGDLKDLE LQADSAIKGR EEAIKQLRKL QAQMKDFQRE LDDARASRDE IFATSKENEK 

      1690       1700       1710       1720       1730       1740 
KAKSLEADLM QLQEDLAAAE RARKQADLEK EELAEELASS LSGRNTLQDE KRRLEARIAQ 

      1750       1760       1770       1780       1790       1800 
LEEELEEEQG NMEAMSDRVR KATLQAEQLS NELATERSTA QKNESARQQL ERQNKELRSK 

      1810       1820       1830       1840       1850       1860 
LQEVEGAVKA KLKSTVAALE AKIAQLEEQV EQEAREKQAA TKSLKQKDKK LKEVLLQVED 

      1870       1880       1890       1900       1910       1920 
ERKMAEQYKE QAEKGNTKVK QLKRQLEEAE EESQCINANR RKLQRELDEA TESNEAMGRE 

      1930       1940       1950       1960       1970 
VNALKSKLRR GNEASFVPSR RAGGRRVIEN TDGSEEEMDA RDSDFNGTKA SE

« Hide

Isoform 2 [UniParc].

Checksum: 46A0B1B9BCB63A8A
Show »

FASTA1,938223,266

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of murine smooth muscle myosin heavy chains."
Hasegawa K., Arakawa E., Oda S., Matsuda Y.
Biochem. Biophys. Res. Commun. 232:313-316(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Uterus.
[2]"Smooth muscle myosin heavy chain exclusively marks the smooth muscle lineage during mouse embryogenesis."
Miano J.M., Cserjesi P., Ligon K.L., Periasamy M., Olson E.N.
Circ. Res. 75:803-812(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-368.
Tissue: Uterus.
[3]"Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2."
Suzuki T., Kim H.S., Kurabayashi M., Hamada H., Fujii H., Aikawa M., Watanabe M., Watanabe N., Sakomura Y., Yazaki Y., Nagai R.
Circ. Res. 78:395-404(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-126.
Tissue: Uterus.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1218-1224, MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.
J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1954, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D85923 mRNA. Translation: BAA19690.1.
D85924 mRNA. Translation: BAA19691.1.
L25860 mRNA. Translation: AAA67552.1.
S81516 mRNA. Translation: AAB36168.1.
IPIIPI00114894.
IPI00227865.
PIRI52863.
JC5420.
JC5421.
UniGeneMm.250705.

3D structure databases

ProteinModelPortalO08638.
SMRO08638. Positions 7-963.
ModBaseSearch...

Protein-protein interaction databases

IntActO08638. 3 interactions.

PTM databases

PhosphoSiteO08638.

Proteomic databases

PaxDbO08638.
PRIDEO08638.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:102643. Myh11.

Phylogenomic databases

eggNOGCOG5022.
HOGENOMHOG000173958.
HOVERGENHBG004704.
InParanoidO08638.
OrthoDBEOG4TXBR1.

Gene expression databases

CleanExMM_MYH11.
GenevestigatorO08638.
GermOnlineENSMUSG00000018830. Mus musculus.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYH11. mouse.
SOURCESearch...

Entry information

Entry nameMYH11_MOUSE
AccessionPrimary (citable) accession number: O08638
Secondary accession number(s): O08639, Q62462, Q64195
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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