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Protein

Synaptotagmin-10

Gene

Syt10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Ca2+ sensor specifically required for the Ca2+-dependent exocytosis of secretory vesicles containing IGF1 in neurons of the olfactory bulb. Exocytosis of IGF1 is required for sensory perception of smell. Not involved in Ca2+-dependent synaptic vesicle exocytosis (By similarity). Acts through Ca2+ and phospholipid binding to the C2 domain: Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (PubMed:18508778).By similarity1 Publication

Cofactori

Ca2+1 PublicationNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi262 – 2621Calcium 1By similarity
Metal bindingi262 – 2621Calcium 2By similarity
Metal bindingi268 – 2681Calcium 1By similarity
Metal bindingi320 – 3201Calcium 1By similarity
Metal bindingi320 – 3201Calcium 2By similarity
Metal bindingi321 – 3211Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi322 – 3221Calcium 1By similarity
Metal bindingi322 – 3221Calcium 2By similarity
Metal bindingi322 – 3221Calcium 3By similarity
Metal bindingi325 – 3251Calcium 3By similarity
Metal bindingi328 – 3281Calcium 2By similarity
Metal bindingi328 – 3281Calcium 3By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-10
Alternative name(s):
Synaptotagmin X
Short name:
SytX
Gene namesi
Name:Syt10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi62041. Syt10.

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesicle membrane By similarity; Single-pass membrane protein Sequence analysis

  • Note: Localizes to neuronal vesicles containing IGF1 that are not enriched at synapses. Does not colocalize with synaptic vesicles or with the Golgi apparatus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555VesicularSequence analysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence analysisAdd
BLAST
Topological domaini77 – 523447CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Synaptotagmin-10PRO_0000183968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 1361PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO08625.
PRIDEiO08625.

Expressioni

Gene expression databases

GenevisibleiO08625. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif. Can also form heterodimers with SYT3, SYT6, SYT7 and SYT9.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019406.

Structurei

3D structure databases

ProteinModelPortaliO08625.
SMRiO08625. Positions 232-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini233 – 334102C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini365 – 468104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 3523Cysteine motifBy similarityAdd
BLAST

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity
The first C2 domain mediates Ca2+-dependent phospholipid binding.1 Publication

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO08625.
KOiK19910.
OMAiPPENVEQ.
OrthoDBiEOG78PV8W.
PhylomeDBiO08625.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028677. SYT10.
[Graphical view]
PANTHERiPTHR10024:SF46. PTHR10024:SF46. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRKEDGVS SLCQKALHII TELCFAGQVE WDKCSGIFPA DRSGQGGGGT
60 70 80 90 100
DISVSLLAVV VSFCGLALLV VSLFVFWKLC WPCWKSKLVA PNVSTLPQSI
110 120 130 140 150
SSAPTEVFET EEKKEVEENE KPAPKAIEPA IKISHTSPDI PAEVQTALKE
160 170 180 190 200
HLIKHARVQR QTTDPTSSSR HNSFRRHLPR QMNVSSVDFS MGTEPVLQRG
210 220 230 240 250
ETRTSIGRIK PELYKQKSVD SEGNRKDDVK TCGKLNFALQ YDYENELLVV
260 270 280 290 300
KIIKALDLPA KDFTGTSDPY VKIYLLPDRK KKFQTRVHRK TLNPLFDELF
310 320 330 340 350
QFPVVYDQLS NRKLHFSIYD FDRFSRHDMI GEVILDNLFE VSDLSREATV
360 370 380 390 400
WKDIHCATTE SIDLGEIMFS LCYLPTAGRM TLTVIKCRNL KAMDITGSSD
410 420 430 440 450
PYVKVSLMCE GRRLKKRKTT TKKNTLNPVY NEAIIFDIPP ENVDQVSLCI
460 470 480 490 500
AVMDYDRVGH NEVIGVCRTG LDAEGLGRDH WNEMLAYHRK PITHWHPLLE
510 520
LPGRATSFDS QGSCSSPRPP STP
Length:523
Mass (Da):59,011
Last modified:July 11, 2003 - v2
Checksum:i48F42A2D19517A3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631F → S in AAB51686 (PubMed:9122248).Curated
Sequence conflicti362 – 3621I → M in AAB51686 (PubMed:9122248).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375463 mRNA. Translation: AAK56958.1.
U85513 mRNA. Translation: AAB51686.1.
PIRiPC6300.
RefSeqiNP_113854.1. NM_031666.1.
UniGeneiRn.212261.

Genome annotation databases

EnsembliENSRNOT00000019406; ENSRNOP00000019406; ENSRNOG00000014296.
GeneIDi60567.
KEGGirno:60567.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375463 mRNA. Translation: AAK56958.1.
U85513 mRNA. Translation: AAB51686.1.
PIRiPC6300.
RefSeqiNP_113854.1. NM_031666.1.
UniGeneiRn.212261.

3D structure databases

ProteinModelPortaliO08625.
SMRiO08625. Positions 232-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019406.

Proteomic databases

PaxDbiO08625.
PRIDEiO08625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019406; ENSRNOP00000019406; ENSRNOG00000014296.
GeneIDi60567.
KEGGirno:60567.

Organism-specific databases

CTDi341359.
RGDi62041. Syt10.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO08625.
KOiK19910.
OMAiPPENVEQ.
OrthoDBiEOG78PV8W.
PhylomeDBiO08625.
TreeFamiTF315600.

Miscellaneous databases

NextBioi612258.
PROiO08625.

Gene expression databases

GenevisibleiO08625. RN.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028677. SYT10.
[Graphical view]
PANTHERiPTHR10024:SF46. PTHR10024:SF46. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Shin O.-H., Suedhof T.C.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A novel seizure-induced synaptotagmin gene identified by differential display."
    Babity J.M., Armstrong J.N., Plumier J.C., Currie R.W., Robertson H.A.
    Proc. Natl. Acad. Sci. U.S.A. 94:2638-2641(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-498.
    Strain: Sprague-Dawley.
  3. "Synaptotagmins form a hierarchy of exocytotic Ca(2+) sensors with distinct Ca(2+) affinities."
    Sugita S., Shin O.H., Han W., Lao Y., Suedhof T.C.
    EMBO J. 21:270-280(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, DOMAIN.
  4. "Analysis of the synaptotagmin family during reconstituted membrane fusion. Uncovering a class of inhibitory isoforms."
    Bhalla A., Chicka M.C., Chapman E.R.
    J. Biol. Chem. 283:21799-21807(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSYT10_RAT
AccessioniPrimary (citable) accession number: O08625
Secondary accession number(s): Q925B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 11, 2003
Last modified: May 11, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.