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Protein

Synaptotagmin-10

Gene

Syt10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ca2+ sensor specifically required for the Ca2+-dependent exocytosis of secretory vesicles containing IGF1 in neurons of the olfactory bulb. Exocytosis of IGF1 is required for sensory perception of smell. Not involved in Ca2+-dependent synaptic vesicle exocytosis (By similarity). Acts through Ca2+ and phospholipid binding to the C2 domain: Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (PubMed:18508778).By similarity1 Publication

Cofactori

Ca2+1 PublicationNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262Calcium 1By similarity1
Metal bindingi262Calcium 2By similarity1
Metal bindingi268Calcium 1By similarity1
Metal bindingi320Calcium 1By similarity1
Metal bindingi320Calcium 2By similarity1
Metal bindingi321Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi322Calcium 1By similarity1
Metal bindingi322Calcium 2By similarity1
Metal bindingi322Calcium 3By similarity1
Metal bindingi325Calcium 3By similarity1
Metal bindingi328Calcium 2By similarity1
Metal bindingi328Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-10
Alternative name(s):
Synaptotagmin X
Short name:
SytX
Gene namesi
Name:Syt10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi62041. Syt10.

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesicle membrane By similarity; Single-pass membrane protein Sequence analysis

  • Note: Localizes to neuronal vesicles containing IGF1 that are not enriched at synapses. Does not colocalize with synaptic vesicles or with the Golgi apparatus.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55VesicularSequence analysisAdd BLAST55
Transmembranei56 – 76HelicalSequence analysisAdd BLAST21
Topological domaini77 – 523CytoplasmicSequence analysisAdd BLAST447

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839681 – 523Synaptotagmin-10Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO08625.
PRIDEiO08625.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014296.
GenevisibleiO08625. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif. Can also form heterodimers with SYT3, SYT6, SYT7 and SYT9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019406.

Structurei

3D structure databases

ProteinModelPortaliO08625.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 334C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini365 – 468C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 35Cysteine motifBy similarityAdd BLAST23

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity
The first C2 domain mediates Ca2+-dependent phospholipid binding.1 Publication

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO08625.
KOiK19910.
OMAiPPENVEQ.
OrthoDBiEOG091G0XMQ.
PhylomeDBiO08625.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028677. SYT10.
[Graphical view]
PANTHERiPTHR10024:SF46. PTHR10024:SF46. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRKEDGVS SLCQKALHII TELCFAGQVE WDKCSGIFPA DRSGQGGGGT
60 70 80 90 100
DISVSLLAVV VSFCGLALLV VSLFVFWKLC WPCWKSKLVA PNVSTLPQSI
110 120 130 140 150
SSAPTEVFET EEKKEVEENE KPAPKAIEPA IKISHTSPDI PAEVQTALKE
160 170 180 190 200
HLIKHARVQR QTTDPTSSSR HNSFRRHLPR QMNVSSVDFS MGTEPVLQRG
210 220 230 240 250
ETRTSIGRIK PELYKQKSVD SEGNRKDDVK TCGKLNFALQ YDYENELLVV
260 270 280 290 300
KIIKALDLPA KDFTGTSDPY VKIYLLPDRK KKFQTRVHRK TLNPLFDELF
310 320 330 340 350
QFPVVYDQLS NRKLHFSIYD FDRFSRHDMI GEVILDNLFE VSDLSREATV
360 370 380 390 400
WKDIHCATTE SIDLGEIMFS LCYLPTAGRM TLTVIKCRNL KAMDITGSSD
410 420 430 440 450
PYVKVSLMCE GRRLKKRKTT TKKNTLNPVY NEAIIFDIPP ENVDQVSLCI
460 470 480 490 500
AVMDYDRVGH NEVIGVCRTG LDAEGLGRDH WNEMLAYHRK PITHWHPLLE
510 520
LPGRATSFDS QGSCSSPRPP STP
Length:523
Mass (Da):59,011
Last modified:July 11, 2003 - v2
Checksum:i48F42A2D19517A3B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263F → S in AAB51686 (PubMed:9122248).Curated1
Sequence conflicti362I → M in AAB51686 (PubMed:9122248).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375463 mRNA. Translation: AAK56958.1.
U85513 mRNA. Translation: AAB51686.1.
PIRiPC6300.
RefSeqiNP_113854.1. NM_031666.1.
UniGeneiRn.212261.

Genome annotation databases

EnsembliENSRNOT00000019406; ENSRNOP00000019406; ENSRNOG00000014296.
GeneIDi60567.
KEGGirno:60567.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375463 mRNA. Translation: AAK56958.1.
U85513 mRNA. Translation: AAB51686.1.
PIRiPC6300.
RefSeqiNP_113854.1. NM_031666.1.
UniGeneiRn.212261.

3D structure databases

ProteinModelPortaliO08625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019406.

Proteomic databases

PaxDbiO08625.
PRIDEiO08625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019406; ENSRNOP00000019406; ENSRNOG00000014296.
GeneIDi60567.
KEGGirno:60567.

Organism-specific databases

CTDi341359.
RGDi62041. Syt10.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO08625.
KOiK19910.
OMAiPPENVEQ.
OrthoDBiEOG091G0XMQ.
PhylomeDBiO08625.
TreeFamiTF315600.

Miscellaneous databases

PROiO08625.

Gene expression databases

BgeeiENSRNOG00000014296.
GenevisibleiO08625. RN.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028677. SYT10.
[Graphical view]
PANTHERiPTHR10024:SF46. PTHR10024:SF46. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT10_RAT
AccessioniPrimary (citable) accession number: O08625
Secondary accession number(s): Q925B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 11, 2003
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.