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Protein

Receptor-type tyrosine-protein phosphatase R

Gene

Ptprr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531SubstrateBy similarity
Active sitei587 – 5871Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei631 – 6311SubstrateBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: RGD

GO - Biological processi

  1. in utero embryonic development Source: UniProtKB
  2. nervous system development Source: RGD
  3. neuron differentiation Source: RGD
  4. peptidyl-tyrosine dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase R (EC:3.1.3.48)
Short name:
R-PTP-R
Alternative name(s):
PC12-PTP1
Protein-tyrosine phosphatase PCPTP1
Tyrosine phosphatase CBPTP
Gene namesi
Name:Ptprr
Synonyms:Ptp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620780. Ptprr.

Subcellular locationi

Isoform 3 :
  1. Cytoplasm By similarity
Isoform 2 :
  1. Cytoplasm By similarity
Isoform 1 :
  1. Cytoplasm By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 225201ExtracellularSequence AnalysisAdd
BLAST
Transmembranei226 – 24823HelicalSequence AnalysisAdd
BLAST
Topological domaini249 – 656408CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 656633Receptor-type tyrosine-protein phosphatase RPRO_0000025461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Modified residuei338 – 3381Phosphoserine; by PKABy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO08617.

Expressioni

Tissue specificityi

Widely expressed in the brain, most abundant in cerebellum, midbrain, cerebral cortex and hippocampus. Also expressed in heart and skeletal muscle.2 Publications

Inductioni

By nerve growth factor; isoform 2 is induced in adrenal tumor cells 2 hours after exposure, levels are down-regulated 24 hours after treatment.1 Publication

Gene expression databases

GenevestigatoriO08617.

Interactioni

Subunit structurei

Interacts with MAPKs.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SrcQ9WUD92EBI-8584374,EBI-7784541

Protein-protein interaction databases

BioGridi250182. 1 interaction.
IntActiO08617. 1 interaction.
MINTiMINT-4581737.

Structurei

3D structure databases

ProteinModelPortaliO08617.
SMRiO08617. Positions 360-655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini392 – 646255Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni587 – 5937Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiO08617.
KOiK04458.
PhylomeDBiO08617.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: O08617-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRAVGFPAL CLLLNLHAAG CFSRNNDHFL AIRQKKSWKP MFIYDHSQDI
60 70 80 90 100
KKSLDIAQEA YKHNYPAPSE VQISKRHQIV DSAFPRPAYD PSLNLLAASG
110 120 130 140 150
QDLEIENLPI PAANVIVVTL QMDIDKLNIT LLRIFRQGVA AALGLLPQQV
160 170 180 190 200
HINRLIEKKS QIELFVSPGN RKPGEPQALQ AEEVLRSLNV DVLRQSLPQF
210 220 230 240 250
GSIDVSPEKN VLQGQHEADK IWSKEGFYAV VIFLSIFIII VTCLMIIYRL
260 270 280 290 300
KERLQLSFRQ DKEKNQEIHL SPIALQQAQS EAKAAHSMVQ PDQAPKVLNV
310 320 330 340 350
VVDPQGQCTP EIRNTASTSV CPSPFRMKPI GLQERRGSNV SLTLDMSSLG
360 370 380 390 400
NVEPFVAVST PREKVAMEYL QSASRVLTSP QLRDVVASSH LLQSEFMEIP
410 420 430 440 450
MNFVDPKEID IPRHGTKNRY KTILPNPLSR VCLRPKNITD PLSTYINANY
460 470 480 490 500
IRGYSGKEKA FIATQGPMIN TVNDFWQMVW QEDSPVIVMI TKLKEKNEKC
510 520 530 540 550
VLYWPEKRGI YGKVEVLVIG VNECDNYTIR NLVLKRGSHT QHVKHYWYTS
560 570 580 590 600
WPDHKTPDSA QPLLQLMLDV EEDRLASEGR GPVVVHCSAG IGRTGCFIAT
610 620 630 640 650
SIGCQQLKEE GVVDALSIVC QLRVDRGGMV QTSEQYEFVH HALCLFESRL

SPETVQ
Length:656
Mass (Da):73,907
Last modified:November 15, 2002 - v2
Checksum:iE929E5473B6A9DE9
GO
Isoform 1 (identifier: O08617-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Show »
Length:535
Mass (Da):60,317
Checksum:i7412831F3C61670F
GO
Isoform 2 (identifier: O08617-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Show »
Length:412
Mass (Da):46,530
Checksum:i0AFCF5266F96680D
GO
Isoform 3 (identifier: O08617-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-287: Missing.

Show »
Length:369
Mass (Da):41,500
Checksum:iEE02807E41EC871A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021S → I in BAA19530 (PubMed:7557444).Curated
Sequence conflicti258 – 2581F → L (PubMed:7557444).Curated
Sequence conflicti258 – 2581F → L (PubMed:7814416).Curated
Sequence conflicti379 – 3791S → R (PubMed:7557444).Curated
Sequence conflicti379 – 3791S → R (PubMed:7814416).Curated
Sequence conflicti510 – 5112IY → TH in AAA64485 (PubMed:7814416).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 287287Missing in isoform 3. 1 PublicationVSP_005164Add
BLAST
Alternative sequencei1 – 244244Missing in isoform 2. 1 PublicationVSP_005163Add
BLAST
Alternative sequencei1 – 121121Missing in isoform 1. 1 PublicationVSP_005162Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64050 mRNA. Translation: BAA19530.1.
D38292 mRNA. Translation: BAA07414.1.
U14914 mRNA. Translation: AAA64485.1.
PIRiJC4263.
RefSeqiNP_001106861.1. NM_001113390.1.
NP_446046.2. NM_053594.2.
UniGeneiRn.6277.

Genome annotation databases

GeneIDi94202.
KEGGirno:94202.
UCSCiRGD:620780. rat. [O08617-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64050 mRNA. Translation: BAA19530.1.
D38292 mRNA. Translation: BAA07414.1.
U14914 mRNA. Translation: AAA64485.1.
PIRiJC4263.
RefSeqiNP_001106861.1. NM_001113390.1.
NP_446046.2. NM_053594.2.
UniGeneiRn.6277.

3D structure databases

ProteinModelPortaliO08617.
SMRiO08617. Positions 360-655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250182. 1 interaction.
IntActiO08617. 1 interaction.
MINTiMINT-4581737.

Proteomic databases

PRIDEiO08617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi94202.
KEGGirno:94202.
UCSCiRGD:620780. rat. [O08617-1]

Organism-specific databases

CTDi5801.
RGDi620780. Ptprr.

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiO08617.
KOiK04458.
PhylomeDBiO08617.

Miscellaneous databases

NextBioi617875.
PROiO08617.

Gene expression databases

GenevestigatoriO08617.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of PCPTP1 encoding protein tyrosine phosphatase."
    Shiozuka K., Watanabe Y., Ikeda T., Hashimoto S., Kawashima H.
    Gene 162:279-284(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 4), TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Cerebellum.
  2. "A neuronal protein tyrosine phosphatase induced by nerve growth factor."
    Sharma E., Lombroso P.J.
    J. Biol. Chem. 270:49-53(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INDUCTION, TISSUE SPECIFICITY.
    Tissue: Adrenal tumor.

Entry informationi

Entry nameiPTPRR_RAT
AccessioniPrimary (citable) accession number: O08617
Secondary accession number(s): Q62695, Q63419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: January 7, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.