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O08609

- MLX_MOUSE

UniProt

O08609 - MLX_MOUSE

Protein

Max-like protein X

Gene

Mlx

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.3 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. nucleocytoplasmic transport Source: MGI
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Max-like protein X
    Alternative name(s):
    Max-like bHLHZip protein
    Protein BigMax
    Transcription factor-like protein 4
    Gene namesi
    Name:Mlx
    Synonyms:Tcfl4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:108398. Mlx.

    Subcellular locationi

    Isoform Alpha : Cytoplasm By similarity
    Note: Found predominantly in the cytoplasm.By similarity
    Isoform Beta : Cytoplasm By similarity
    Note: Found predominantly in the cytoplasm.By similarity
    Isoform Gamma : Nucleus PROSITE-ProRule annotation
    Note: Found predominantly in the nucleus.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleoplasm Source: Reactome
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Max-like protein XPRO_0000127280Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451Phosphoserine1 Publication
    Modified residuei48 – 481Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO08609.
    PRIDEiO08609.

    PTM databases

    PhosphoSiteiO08609.

    Expressioni

    Tissue specificityi

    Expressed in all tissues examined: stomach, duodenum, jejunum, ileum, colon, liver, pancreas, salivary gland, kidney, spleen, lung, heart, skeletal muscle, brain, ovary and testis.1 Publication

    Developmental stagei

    Expression peaks between dpc 9.5 to 11.5, then decreases during later stages of organogenesis.1 Publication

    Gene expression databases

    CleanExiMM_MLX.
    GenevestigatoriO08609.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.

    Protein-protein interaction databases

    BioGridi204019. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO08609.
    SMRiO08609. Positions 125-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini129 – 18759bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 21421Leucine-zipperAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG259028.
    HOGENOMiHOG000232118.
    HOVERGENiHBG019061.
    InParanoidiO08609.
    KOiK09113.
    PhylomeDBiO08609.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Gamma (identifier: O08609-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTEPGASPED PWVKASFADA HAGEGRAGRA RARRGSGRRG APQLSPESPL    50
    LSGARGCRED SSHPACAKVE YAYSDNSLDP GLFVESTHKG SVVSRANSIG 100
    STSASSVPNT DDEDSDYQQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD 150
    DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST 200
    LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF 250
    QSFNASISVA SFQELSACVF SWIEEHCEPQ TLREIVIGVL HQLKNQLY 298
    Length:298
    Mass (Da):33,339
    Last modified:July 1, 1997 - v1
    Checksum:iA494D5A86292B71C
    GO
    Isoform Alpha (identifier: O08609-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         15-68: Missing.
         81-110: Missing.

    Show »
    Length:214
    Mass (Da):24,897
    Checksum:i7C7690DFF2CEF921
    GO
    Isoform Beta (identifier: O08609-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         15-68: Missing.

    Show »
    Length:244
    Mass (Da):27,856
    Checksum:i1D867FCAA26764B0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei15 – 6854Missing in isoform Alpha and isoform Beta. CuratedVSP_002139Add
    BLAST
    Alternative sequencei81 – 11030Missing in isoform Alpha. CuratedVSP_002140Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43607 mRNA. Translation: AAB51368.1.
    U43548 mRNA. Translation: AAB51367.1.
    AF213670 mRNA. Translation: AAG40149.1.
    AF213671 mRNA. Translation: AAG40150.1.
    AF213672 mRNA. Translation: AAG40151.1.
    CCDSiCCDS25447.1. [O08609-1]
    CCDS48935.1. [O08609-3]
    CCDS48936.1. [O08609-2]
    RefSeqiNP_001152856.1. NM_001159384.1.
    NP_001152857.1. NM_001159385.1.
    NP_035680.3. NM_011550.3.
    UniGeneiMm.628.

    Genome annotation databases

    GeneIDi21428.
    KEGGimmu:21428.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43607 mRNA. Translation: AAB51368.1 .
    U43548 mRNA. Translation: AAB51367.1 .
    AF213670 mRNA. Translation: AAG40149.1 .
    AF213671 mRNA. Translation: AAG40150.1 .
    AF213672 mRNA. Translation: AAG40151.1 .
    CCDSi CCDS25447.1. [O08609-1 ]
    CCDS48935.1. [O08609-3 ]
    CCDS48936.1. [O08609-2 ]
    RefSeqi NP_001152856.1. NM_001159384.1.
    NP_001152857.1. NM_001159385.1.
    NP_035680.3. NM_011550.3.
    UniGenei Mm.628.

    3D structure databases

    ProteinModelPortali O08609.
    SMRi O08609. Positions 125-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204019. 1 interaction.

    PTM databases

    PhosphoSitei O08609.

    Proteomic databases

    PaxDbi O08609.
    PRIDEi O08609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 21428.
    KEGGi mmu:21428.

    Organism-specific databases

    CTDi 6945.
    MGIi MGI:108398. Mlx.

    Phylogenomic databases

    eggNOGi NOG259028.
    HOGENOMi HOG000232118.
    HOVERGENi HBG019061.
    InParanoidi O08609.
    KOi K09113.
    PhylomeDBi O08609.

    Miscellaneous databases

    NextBioi 300758.
    PROi O08609.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_MLX.
    Genevestigatori O08609.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TCFL4: a gene at 17q21.1 encoding a putative basic helix-loop-helix leucine-zipper transcription factor."
      Bjerknes M., Cheng H.
      Gene 181:7-11(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Intestine.
    2. "Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?"
      Meroni G., Cairo S., Merla G., Messali S., Brent R., Ballabio A., Reymond A.
      Oncogene 19:3266-3277(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    3. "Mlx, a novel Max-like bHLHZip protein that interacts with the Max network of transcription factors."
      Billin A.N., Eilers A.L., Queva C., Ayer D.E.
      J. Biol. Chem. 274:36344-36350(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Embryonic stem cell.
    4. "MondoA, a novel basic helix-loop-helix-leucine zipper transcriptional activator that constitutes a positive branch of a max-like network."
      Billin A.N., Eilers A.L., Coulter K.L., Logan J.S., Ayer D.E.
      Mol. Cell. Biol. 20:8845-8854(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MLXIP.
    5. "Glucose-dependent transcriptional regulation by an evolutionarily conserved glucose-sensing module."
      Li M.V., Chang B., Imamura M., Poungvarin N., Chan L.
      Diabetes 55:1179-1189(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiMLX_MOUSE
    AccessioniPrimary (citable) accession number: O08609
    Secondary accession number(s): Q9EQJ7, Q9EQJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3