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Protein

Max-like protein X

Gene

Mlx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_333964. ChREBP activates metabolic gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Max-like protein X
Alternative name(s):
Max-like bHLHZip protein
Protein BigMax
Transcription factor-like protein 4
Gene namesi
Name:Mlx
Synonyms:Tcfl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108398. Mlx.

Subcellular locationi

Isoform Alpha :
  • Cytoplasm By similarity

  • Note: Found predominantly in the cytoplasm.By similarity
Isoform Beta :
  • Cytoplasm By similarity

  • Note: Found predominantly in the cytoplasm.By similarity
Isoform Gamma :
  • Nucleus PROSITE-ProRule annotation

  • Note: Found predominantly in the nucleus.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Max-like protein XPRO_0000127280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451Phosphoserine1 Publication
Modified residuei48 – 481Phosphoserine1 Publication
Modified residuei74 – 741PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO08609.
PaxDbiO08609.
PRIDEiO08609.

PTM databases

PhosphoSiteiO08609.

Expressioni

Tissue specificityi

Expressed in all tissues examined: stomach, duodenum, jejunum, ileum, colon, liver, pancreas, salivary gland, kidney, spleen, lung, heart, skeletal muscle, brain, ovary and testis.1 Publication

Developmental stagei

Expression peaks between dpc 9.5 to 11.5, then decreases during later stages of organogenesis.1 Publication

Gene expression databases

CleanExiMM_MLX.
GenevisibleiO08609. MM.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.

Protein-protein interaction databases

BioGridi204019. 1 interaction.
STRINGi10090.ENSMUSP00000017945.

Structurei

3D structure databases

ProteinModelPortaliO08609.
SMRiO08609. Positions 125-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 18759bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 21421Leucine-zipperAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG259028.
HOGENOMiHOG000232118.
HOVERGENiHBG019061.
InParanoidiO08609.
KOiK09113.
PhylomeDBiO08609.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma (identifier: O08609-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKASFADA HAGEGRAGRA RARRGSGRRG APQLSPESPL
60 70 80 90 100
LSGARGCRED SSHPACAKVE YAYSDNSLDP GLFVESTHKG SVVSRANSIG
110 120 130 140 150
STSASSVPNT DDEDSDYQQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD
160 170 180 190 200
DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST
210 220 230 240 250
LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF
260 270 280 290
QSFNASISVA SFQELSACVF SWIEEHCEPQ TLREIVIGVL HQLKNQLY
Length:298
Mass (Da):33,339
Last modified:July 1, 1997 - v1
Checksum:iA494D5A86292B71C
GO
Isoform Alpha (identifier: O08609-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.
     81-110: Missing.

Show »
Length:214
Mass (Da):24,897
Checksum:i7C7690DFF2CEF921
GO
Isoform Beta (identifier: O08609-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.

Show »
Length:244
Mass (Da):27,856
Checksum:i1D867FCAA26764B0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 6854Missing in isoform Alpha and isoform Beta. CuratedVSP_002139Add
BLAST
Alternative sequencei81 – 11030Missing in isoform Alpha. CuratedVSP_002140Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43607 mRNA. Translation: AAB51368.1.
U43548 mRNA. Translation: AAB51367.1.
AF213670 mRNA. Translation: AAG40149.1.
AF213671 mRNA. Translation: AAG40150.1.
AF213672 mRNA. Translation: AAG40151.1.
CCDSiCCDS25447.1. [O08609-1]
CCDS48935.1. [O08609-3]
CCDS48936.1. [O08609-2]
RefSeqiNP_001152856.1. NM_001159384.1.
NP_001152857.1. NM_001159385.1.
NP_035680.3. NM_011550.3.
UniGeneiMm.628.

Genome annotation databases

GeneIDi21428.
KEGGimmu:21428.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43607 mRNA. Translation: AAB51368.1.
U43548 mRNA. Translation: AAB51367.1.
AF213670 mRNA. Translation: AAG40149.1.
AF213671 mRNA. Translation: AAG40150.1.
AF213672 mRNA. Translation: AAG40151.1.
CCDSiCCDS25447.1. [O08609-1]
CCDS48935.1. [O08609-3]
CCDS48936.1. [O08609-2]
RefSeqiNP_001152856.1. NM_001159384.1.
NP_001152857.1. NM_001159385.1.
NP_035680.3. NM_011550.3.
UniGeneiMm.628.

3D structure databases

ProteinModelPortaliO08609.
SMRiO08609. Positions 125-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204019. 1 interaction.
STRINGi10090.ENSMUSP00000017945.

PTM databases

PhosphoSiteiO08609.

Proteomic databases

MaxQBiO08609.
PaxDbiO08609.
PRIDEiO08609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21428.
KEGGimmu:21428.

Organism-specific databases

CTDi6945.
MGIiMGI:108398. Mlx.

Phylogenomic databases

eggNOGiNOG259028.
HOGENOMiHOG000232118.
HOVERGENiHBG019061.
InParanoidiO08609.
KOiK09113.
PhylomeDBiO08609.

Enzyme and pathway databases

ReactomeiREACT_333964. ChREBP activates metabolic gene expression.

Miscellaneous databases

NextBioi300758.
PROiO08609.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MLX.
GenevisibleiO08609. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TCFL4: a gene at 17q21.1 encoding a putative basic helix-loop-helix leucine-zipper transcription factor."
    Bjerknes M., Cheng H.
    Gene 181:7-11(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Intestine.
  2. "Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?"
    Meroni G., Cairo S., Merla G., Messali S., Brent R., Ballabio A., Reymond A.
    Oncogene 19:3266-3277(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  3. "Mlx, a novel Max-like bHLHZip protein that interacts with the Max network of transcription factors."
    Billin A.N., Eilers A.L., Queva C., Ayer D.E.
    J. Biol. Chem. 274:36344-36350(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryonic stem cell.
  4. "MondoA, a novel basic helix-loop-helix-leucine zipper transcriptional activator that constitutes a positive branch of a max-like network."
    Billin A.N., Eilers A.L., Coulter K.L., Logan J.S., Ayer D.E.
    Mol. Cell. Biol. 20:8845-8854(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MLXIP.
  5. "Glucose-dependent transcriptional regulation by an evolutionarily conserved glucose-sensing module."
    Li M.V., Chang B., Imamura M., Poungvarin N., Chan L.
    Diabetes 55:1179-1189(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMLX_MOUSE
AccessioniPrimary (citable) accession number: O08609
Secondary accession number(s): Q9EQJ7, Q9EQJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 1, 1997
Last modified: July 22, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.