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O08609 (MLX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Max-like protein X
Alternative name(s):
Max-like bHLHZip protein
Protein BigMax
Transcription factor-like protein 4
Gene names
Name:Mlx
Synonyms:Tcfl4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. Ref.3 Ref.4 Ref.5

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.

Subcellular location

Isoform Alpha: Cytoplasm By similarity. Note: Found predominantly in the cytoplasm By similarity.

Isoform Beta: Cytoplasm By similarity. Note: Found predominantly in the cytoplasm By similarity.

Isoform Gamma: Nucleus By similarity. Note: Found predominantly in the nucleus.

Tissue specificity

Expressed in all tissues examined: stomach, duodenum, jejunum, ileum, colon, liver, pancreas, salivary gland, kidney, spleen, lung, heart, skeletal muscle, brain, ovary and testis. Ref.1

Developmental stage

Expression peaks between dpc 9.5 to 11.5, then decreases during later stages of organogenesis. Ref.3

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Gamma (identifier: O08609-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha (identifier: O08609-2)

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.
     81-110: Missing.
Isoform Beta (identifier: O08609-3)

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Max-like protein X
PRO_0000127280

Regions

Domain129 – 18759bHLH
Region194 – 21421Leucine-zipper

Amino acid modifications

Modified residue451Phosphoserine Ref.6
Modified residue481Phosphoserine Ref.6

Natural variations

Alternative sequence15 – 6854Missing in isoform Alpha and isoform Beta.
VSP_002139
Alternative sequence81 – 11030Missing in isoform Alpha.
VSP_002140

Sequences

Sequence LengthMass (Da)Tools
Isoform Gamma [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: A494D5A86292B71C

FASTA29833,339
        10         20         30         40         50         60 
MTEPGASPED PWVKASFADA HAGEGRAGRA RARRGSGRRG APQLSPESPL LSGARGCRED 

        70         80         90        100        110        120 
SSHPACAKVE YAYSDNSLDP GLFVESTHKG SVVSRANSIG STSASSVPNT DDEDSDYQQE 

       130        140        150        160        170        180 
AYKESYKDRR RRAHTQAEQK RRDAIKRGYD DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT 

       190        200        210        220        230        240 
IDYIQFLHKE KKKQEEEVST LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN 

       250        260        270        280        290 
VFQGIMDSLF QSFNASISVA SFQELSACVF SWIEEHCEPQ TLREIVIGVL HQLKNQLY 

« Hide

Isoform Alpha [UniParc].

Checksum: 7C7690DFF2CEF921
Show »

FASTA21424,897
Isoform Beta [UniParc].

Checksum: 1D867FCAA26764B0
Show »

FASTA24427,856

References

« Hide 'large scale' references
[1]"TCFL4: a gene at 17q21.1 encoding a putative basic helix-loop-helix leucine-zipper transcription factor."
Bjerknes M., Cheng H.
Gene 181:7-11(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Intestine.
[2]"Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?"
Meroni G., Cairo S., Merla G., Messali S., Brent R., Ballabio A., Reymond A.
Oncogene 19:3266-3277(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[3]"Mlx, a novel Max-like bHLHZip protein that interacts with the Max network of transcription factors."
Billin A.N., Eilers A.L., Queva C., Ayer D.E.
J. Biol. Chem. 274:36344-36350(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Embryonic stem cell.
[4]"MondoA, a novel basic helix-loop-helix-leucine zipper transcriptional activator that constitutes a positive branch of a max-like network."
Billin A.N., Eilers A.L., Coulter K.L., Logan J.S., Ayer D.E.
Mol. Cell. Biol. 20:8845-8854(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MLXIP.
[5]"Glucose-dependent transcriptional regulation by an evolutionarily conserved glucose-sensing module."
Li M.V., Chang B., Imamura M., Poungvarin N., Chan L.
Diabetes 55:1179-1189(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43607 mRNA. Translation: AAB51368.1.
U43548 mRNA. Translation: AAB51367.1.
AF213670 mRNA. Translation: AAG40149.1.
AF213671 mRNA. Translation: AAG40150.1.
AF213672 mRNA. Translation: AAG40151.1.
CCDSCCDS25447.1. [O08609-1]
CCDS48935.1. [O08609-3]
CCDS48936.1. [O08609-2]
RefSeqNP_001152856.1. NM_001159384.1.
NP_001152857.1. NM_001159385.1.
NP_035680.3. NM_011550.3.
UniGeneMm.628.

3D structure databases

ProteinModelPortalO08609.
SMRO08609. Positions 125-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204019. 1 interaction.

PTM databases

PhosphoSiteO08609.

Proteomic databases

PaxDbO08609.
PRIDEO08609.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID21428.
KEGGmmu:21428.

Organism-specific databases

CTD6945.
MGIMGI:108398. Mlx.

Phylogenomic databases

eggNOGNOG259028.
HOGENOMHOG000232118.
HOVERGENHBG019061.
InParanoidO08609.
KOK09113.
PhylomeDBO08609.

Enzyme and pathway databases

ReactomeREACT_188937. Metabolism.

Gene expression databases

CleanExMM_MLX.
GenevestigatorO08609.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300758.
PROO08609.
SOURCESearch...

Entry information

Entry nameMLX_MOUSE
AccessionPrimary (citable) accession number: O08609
Secondary accession number(s): Q9EQJ7, Q9EQJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot