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O08605

- MKNK1_MOUSE

UniProt

O08605 - MKNK1_MOUSE

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Protein

MAP kinase-interacting serine/threonine-protein kinase 1

Gene

Mknk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781ATPPROSITE-ProRule annotation
Active sitei170 – 1701Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  2. intracellular signal transduction Source: UniProtKB
  3. peptidyl-serine phosphorylation Source: Ensembl
  4. protein phosphorylation Source: UniProtKB
  5. regulation of translation Source: UniProtKB-KW
  6. response to salt stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198526. Spry regulation of FGF signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
MAP kinase signal-integrating kinase 1
Short name:
MAPK signal-integrating kinase 1
Short name:
Mnk1
Gene namesi
Name:Mknk1
Synonyms:Mnk1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:894316. Mknk1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi209 – 2091T → A: Loss of kinase activity; when associated with T-214. 1 Publication
Mutagenesisi214 – 2141T → A: Loss of kinase activity; when associated with T-209. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427MAP kinase-interacting serine/threonine-protein kinase 1PRO_0000086335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Phosphothreonine; by PAK21 Publication
Modified residuei39 – 391Phosphoserine; by PAK21 Publication
Modified residuei209 – 2091Phosphothreonine1 Publication
Modified residuei214 – 2141Phosphothreonine1 Publication
Modified residuei344 – 3441PhosphothreonineBy similarity

Post-translational modificationi

Dual phosphorylation of Thr-209 and Thr-214 activates the kinase. Phosphorylation of Thr-344 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO08605.
PaxDbiO08605.
PRIDEiO08605.

PTM databases

PhosphoSiteiO08605.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined, with high levels in skeletal muscle.1 Publication

Gene expression databases

BgeeiO08605.
CleanExiMM_MKNK1.
ExpressionAtlasiO08605. baseline and differential.
GenevestigatoriO08605.

Interactioni

Subunit structurei

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.1 Publication

Protein-protein interaction databases

IntActiO08605. 1 interaction.
MINTiMINT-1524139.

Structurei

3D structure databases

ProteinModelPortaliO08605.
SMRiO08605. Positions 39-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 333285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000231140.
HOVERGENiHBG106949.
InParanoidiO08605.
KOiK04372.
PhylomeDBiO08605.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSSQKLEKP IEMGSSEPLP IVDSDKRRKK KRKTRATDSL PGKFEDVYQL
60 70 80 90 100
TSELLGEGAY AKVQGAVNLQ SGKEYAVKII EKQAGHSRSR VFREVETLYQ
110 120 130 140 150
CQGNRNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKRK HFNEREASRV
160 170 180 190 200
VRDVATALDF LHTKGIAHRD LKPENILCES PEKVSPVKIC DFDLGSGVKL
210 220 230 240 250
NNSCTPITTP ELTTPCGSAE YMAPEVVEVF RDEATFYDKR CDLWSLGVVL
260 270 280 290 300
YIMLSGYPPF VGHCGADCGW DRGEVCRMCQ NKLFESIQEG KYEFPDKDWA
310 320 330 340 350
HISNEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPER GLPTPQVLQR
360 370 380 390 400
NSSTMDLTLF AAEAIALNRQ LSQHEENELA EEQEALAEGL CSMKLSPPSK
410 420
SRLARRRALA QAGRSRDANP CLTPAGL
Length:427
Mass (Da):47,915
Last modified:April 30, 2003 - v2
Checksum:i7D59A1CBEE4DF68F
GO

Sequence cautioni

The sequence AAH21369.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021369 mRNA. Translation: AAH21369.1. Different initiation.
Y11091 mRNA. Translation: CAA71965.1.
RefSeqiNP_001272417.1. NM_001285488.1.
NP_067436.1. NM_021461.5.
UniGeneiMm.209327.

Genome annotation databases

GeneIDi17346.
KEGGimmu:17346.
UCSCiuc008ufl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021369 mRNA. Translation: AAH21369.1 . Different initiation.
Y11091 mRNA. Translation: CAA71965.1 .
RefSeqi NP_001272417.1. NM_001285488.1.
NP_067436.1. NM_021461.5.
UniGenei Mm.209327.

3D structure databases

ProteinModelPortali O08605.
SMRi O08605. Positions 39-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O08605. 1 interaction.
MINTi MINT-1524139.

PTM databases

PhosphoSitei O08605.

Proteomic databases

MaxQBi O08605.
PaxDbi O08605.
PRIDEi O08605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17346.
KEGGi mmu:17346.
UCSCi uc008ufl.1. mouse.

Organism-specific databases

CTDi 8569.
MGIi MGI:894316. Mknk1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000231140.
HOVERGENi HBG106949.
InParanoidi O08605.
KOi K04372.
PhylomeDBi O08605.

Enzyme and pathway databases

Reactomei REACT_198526. Spry regulation of FGF signaling.

Miscellaneous databases

NextBioi 291932.
PROi O08605.
SOURCEi Search...

Gene expression databases

Bgeei O08605.
CleanExi MM_MKNK1.
ExpressionAtlasi O08605. baseline and differential.
Genevestigatori O08605.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  2. "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2."
    Waskiewicz A.J., Flynn A., Proud C.G., Cooper J.A.
    EMBO J. 16:1909-1920(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-427, FUNCTION, INTERACTION WITH MAPK3; MAPK1 AND P38 KINASE, PHOSPHORYLATION AT THR-209 AND THR-214, MUTAGENESIS OF THR-209 AND THR-214.
    Tissue: Embryo.
  3. "Phosphorylation of Mnk1 by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk."
    Orton K.C., Ling J., Waskiewicz A.J., Cooper J.A., Merrick W.C., Korneeva N.L., Rhoads R.E., Sonenberg N., Traugh J.A.
    J. Biol. Chem. 279:38649-38657(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-34 AND SER-39 BY PAK2.

Entry informationi

Entry nameiMKNK1_MOUSE
AccessioniPrimary (citable) accession number: O08605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: November 26, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3