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O08605

- MKNK1_MOUSE

UniProt

O08605 - MKNK1_MOUSE

Protein

MAP kinase-interacting serine/threonine-protein kinase 1

Gene

Mknk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781ATPPROSITE-ProRule annotation
    Active sitei170 – 1701Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    2. intracellular signal transduction Source: UniProtKB
    3. peptidyl-serine phosphorylation Source: Ensembl
    4. protein phosphorylation Source: UniProtKB
    5. regulation of translation Source: UniProtKB-KW
    6. response to salt stress Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Translation regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198526. Spry regulation of FGF signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.1)
    Alternative name(s):
    MAP kinase signal-integrating kinase 1
    Short name:
    MAPK signal-integrating kinase 1
    Short name:
    Mnk1
    Gene namesi
    Name:Mknk1
    Synonyms:Mnk1
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:894316. Mknk1.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi209 – 2091T → A: Loss of kinase activity; when associated with T-214. 1 Publication
    Mutagenesisi214 – 2141T → A: Loss of kinase activity; when associated with T-209. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427MAP kinase-interacting serine/threonine-protein kinase 1PRO_0000086335Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei34 – 341Phosphothreonine; by PAK21 Publication
    Modified residuei39 – 391Phosphoserine; by PAK21 Publication
    Modified residuei209 – 2091Phosphothreonine1 Publication
    Modified residuei214 – 2141Phosphothreonine1 Publication
    Modified residuei344 – 3441PhosphothreonineBy similarity

    Post-translational modificationi

    Dual phosphorylation of Thr-209 and Thr-214 activates the kinase. Phosphorylation of Thr-344 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO08605.
    PaxDbiO08605.
    PRIDEiO08605.

    PTM databases

    PhosphoSiteiO08605.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in all tissues examined, with high levels in skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiO08605.
    BgeeiO08605.
    CleanExiMM_MKNK1.
    GenevestigatoriO08605.

    Interactioni

    Subunit structurei

    Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.1 Publication

    Protein-protein interaction databases

    IntActiO08605. 1 interaction.
    MINTiMINT-1524139.

    Structurei

    3D structure databases

    ProteinModelPortaliO08605.
    SMRiO08605. Positions 39-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 333285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000231140.
    HOVERGENiHBG106949.
    InParanoidiO08605.
    KOiK04372.
    PhylomeDBiO08605.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08605-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSSQKLEKP IEMGSSEPLP IVDSDKRRKK KRKTRATDSL PGKFEDVYQL    50
    TSELLGEGAY AKVQGAVNLQ SGKEYAVKII EKQAGHSRSR VFREVETLYQ 100
    CQGNRNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKRK HFNEREASRV 150
    VRDVATALDF LHTKGIAHRD LKPENILCES PEKVSPVKIC DFDLGSGVKL 200
    NNSCTPITTP ELTTPCGSAE YMAPEVVEVF RDEATFYDKR CDLWSLGVVL 250
    YIMLSGYPPF VGHCGADCGW DRGEVCRMCQ NKLFESIQEG KYEFPDKDWA 300
    HISNEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPER GLPTPQVLQR 350
    NSSTMDLTLF AAEAIALNRQ LSQHEENELA EEQEALAEGL CSMKLSPPSK 400
    SRLARRRALA QAGRSRDANP CLTPAGL 427
    Length:427
    Mass (Da):47,915
    Last modified:April 30, 2003 - v2
    Checksum:i7D59A1CBEE4DF68F
    GO

    Sequence cautioni

    The sequence AAH21369.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021369 mRNA. Translation: AAH21369.1. Different initiation.
    Y11091 mRNA. Translation: CAA71965.1.
    RefSeqiNP_001272417.1. NM_001285488.1.
    NP_067436.1. NM_021461.5.
    UniGeneiMm.209327.

    Genome annotation databases

    GeneIDi17346.
    KEGGimmu:17346.
    UCSCiuc008ufl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021369 mRNA. Translation: AAH21369.1 . Different initiation.
    Y11091 mRNA. Translation: CAA71965.1 .
    RefSeqi NP_001272417.1. NM_001285488.1.
    NP_067436.1. NM_021461.5.
    UniGenei Mm.209327.

    3D structure databases

    ProteinModelPortali O08605.
    SMRi O08605. Positions 39-373.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O08605. 1 interaction.
    MINTi MINT-1524139.

    PTM databases

    PhosphoSitei O08605.

    Proteomic databases

    MaxQBi O08605.
    PaxDbi O08605.
    PRIDEi O08605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17346.
    KEGGi mmu:17346.
    UCSCi uc008ufl.1. mouse.

    Organism-specific databases

    CTDi 8569.
    MGIi MGI:894316. Mknk1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000231140.
    HOVERGENi HBG106949.
    InParanoidi O08605.
    KOi K04372.
    PhylomeDBi O08605.

    Enzyme and pathway databases

    Reactomei REACT_198526. Spry regulation of FGF signaling.

    Miscellaneous databases

    ChiTaRSi MKNK1. mouse.
    NextBioi 291932.
    PROi O08605.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08605.
    Bgeei O08605.
    CleanExi MM_MKNK1.
    Genevestigatori O08605.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    2. "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2."
      Waskiewicz A.J., Flynn A., Proud C.G., Cooper J.A.
      EMBO J. 16:1909-1920(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-427, FUNCTION, INTERACTION WITH MAPK3; MAPK1 AND P38 KINASE, PHOSPHORYLATION AT THR-209 AND THR-214, MUTAGENESIS OF THR-209 AND THR-214.
      Tissue: Embryo.
    3. "Phosphorylation of Mnk1 by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk."
      Orton K.C., Ling J., Waskiewicz A.J., Cooper J.A., Merrick W.C., Korneeva N.L., Rhoads R.E., Sonenberg N., Traugh J.A.
      J. Biol. Chem. 279:38649-38657(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-34 AND SER-39 BY PAK2.

    Entry informationi

    Entry nameiMKNK1_MOUSE
    AccessioniPrimary (citable) accession number: O08605
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3