UniProtKB - O08605 (MKNK1_MOUSE)
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Protein
MAP kinase-interacting serine/threonine-protein kinase 1
Gene
Mknk1
Organism
Mus musculus (Mouse)
Status
Functioni
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.1 Publication
Catalytic activityi
ATP + a protein = ADP + a phosphoprotein.1 Publication
Cofactori
Mg2+1 Publication
Enzyme regulationi
Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 170 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 55 – 63 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- calcium-dependent protein serine/threonine kinase activity Source: GO_Central
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- intracellular signal transduction Source: UniProtKB
- peptidyl-serine phosphorylation Source: MGI
- protein autophosphorylation Source: GO_Central
- protein phosphorylation Source: UniProtKB
- regulation of translation Source: UniProtKB-KW
- response to salt stress Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Translation regulation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.11 Publication)Alternative name(s): MAP kinase signal-integrating kinase 1 Short name: MAPK signal-integrating kinase 1 Short name: Mnk1 |
Gene namesi | Name:Mknk1 Synonyms:Mnk1 |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894316. Mknk1. |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 209 | T → A: Loss of kinase activity; when associated with T-214. 1 Publication | 1 | |
Mutagenesisi | 214 | T → A: Loss of kinase activity; when associated with T-209. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086335 | 1 – 427 | MAP kinase-interacting serine/threonine-protein kinase 1Add BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | Phosphothreonine; by PAK21 Publication | 1 | |
Modified residuei | 39 | Phosphoserine; by PAK21 Publication | 1 | |
Modified residuei | 180 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 209 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 214 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 344 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Dual phosphorylation of Thr-209 and Thr-214 activates the kinase. Phosphorylation of Thr-344 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O08605. |
MaxQBi | O08605. |
PaxDbi | O08605. |
PRIDEi | O08605. |
PTM databases
iPTMneti | O08605. |
PhosphoSitePlusi | O08605. |
Expressioni
Tissue specificityi
Ubiquitously expressed in all tissues examined, with high levels in skeletal muscle.1 Publication
Gene expression databases
CleanExi | MM_MKNK1. |
Interactioni
Subunit structurei
Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.1 Publication
Binary interactionsi
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
IntActi | O08605. 4 interactors. |
MINTi | O08605. |
STRINGi | 10090.ENSMUSP00000019677. |
Structurei
3D structure databases
ProteinModelPortali | O08605. |
SMRi | O08605. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 49 – 333 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 285 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0607. Eukaryota. ENOG410XQA9. LUCA. |
HOGENOMi | HOG000231140. |
HOVERGENi | HBG106949. |
InParanoidi | O08605. |
KOi | K04372. |
PhylomeDBi | O08605. |
Family and domain databases
InterProi | View protein in InterPro IPR011009. Kinase-like_dom_sf. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. |
Pfami | View protein in Pfam PF00069. Pkinase. 1 hit. |
SMARTi | View protein in SMART SM00220. S_TKc. 1 hit. |
SUPFAMi | SSF56112. SSF56112. 1 hit. |
PROSITEi | View protein in PROSITE PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. |
i Sequence
Sequence statusi: Complete.
O08605-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSSQKLEKP IEMGSSEPLP IVDSDKRRKK KRKTRATDSL PGKFEDVYQL
60 70 80 90 100
TSELLGEGAY AKVQGAVNLQ SGKEYAVKII EKQAGHSRSR VFREVETLYQ
110 120 130 140 150
CQGNRNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKRK HFNEREASRV
160 170 180 190 200
VRDVATALDF LHTKGIAHRD LKPENILCES PEKVSPVKIC DFDLGSGVKL
210 220 230 240 250
NNSCTPITTP ELTTPCGSAE YMAPEVVEVF RDEATFYDKR CDLWSLGVVL
260 270 280 290 300
YIMLSGYPPF VGHCGADCGW DRGEVCRMCQ NKLFESIQEG KYEFPDKDWA
310 320 330 340 350
HISNEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPER GLPTPQVLQR
360 370 380 390 400
NSSTMDLTLF AAEAIALNRQ LSQHEENELA EEQEALAEGL CSMKLSPPSK
410 420
SRLARRRALA QAGRSRDANP CLTPAGL
Sequence cautioni
The sequence AAH21369 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC021369 mRNA. Translation: AAH21369.1. Different initiation. Y11091 mRNA. Translation: CAA71965.1. |
RefSeqi | NP_001272417.1. NM_001285488.1. NP_067436.1. NM_021461.5. |
UniGenei | Mm.209327. |
Genome annotation databases
GeneIDi | 17346. |
KEGGi | mmu:17346. |
UCSCi | uc008ufl.2. mouse. |
Similar proteinsi
Entry informationi
Entry namei | MKNK1_MOUSE | |
Accessioni | O08605Primary (citable) accession number: O08605 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 30, 2003 |
Last sequence update: | April 30, 2003 | |
Last modified: | March 28, 2018 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |