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Protein

MAP kinase-interacting serine/threonine-protein kinase 1

Gene

Mknk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78ATPPROSITE-ProRule annotation1
Active sitei170Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 63ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • intracellular signal transduction Source: UniProtKB
  • peptidyl-serine phosphorylation Source: MGI
  • protein autophosphorylation Source: GO_Central
  • protein phosphorylation Source: UniProtKB
  • regulation of translation Source: UniProtKB-KW
  • response to salt stress Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processTranslation regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.11 Publication)
Alternative name(s):
MAP kinase signal-integrating kinase 1
Short name:
MAPK signal-integrating kinase 1
Short name:
Mnk1
Gene namesi
Name:Mknk1
Synonyms:Mnk1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:894316 Mknk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209T → A: Loss of kinase activity; when associated with T-214. 1 Publication1
Mutagenesisi214T → A: Loss of kinase activity; when associated with T-209. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863351 – 427MAP kinase-interacting serine/threonine-protein kinase 1Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34Phosphothreonine; by PAK21 Publication1
Modified residuei39Phosphoserine; by PAK21 Publication1
Modified residuei180PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei209Phosphothreonine1 Publication1
Modified residuei214Phosphothreonine1 Publication1
Modified residuei344PhosphothreonineBy similarity1

Post-translational modificationi

Dual phosphorylation of Thr-209 and Thr-214 activates the kinase. Phosphorylation of Thr-344 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO08605
MaxQBiO08605
PaxDbiO08605
PRIDEiO08605

PTM databases

iPTMnetiO08605
PhosphoSitePlusiO08605

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined, with high levels in skeletal muscle.1 Publication

Gene expression databases

CleanExiMM_MKNK1

Interactioni

Subunit structurei

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiO08605, 1 interactor
MINTiO08605
STRINGi10090.ENSMUSP00000019677

Structurei

3D structure databases

ProteinModelPortaliO08605
SMRiO08605
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 333Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0607 Eukaryota
ENOG410XQA9 LUCA
HOGENOMiHOG000231140
HOVERGENiHBG106949
InParanoidiO08605
KOiK04372
PhylomeDBiO08605

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O08605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSQKLEKP IEMGSSEPLP IVDSDKRRKK KRKTRATDSL PGKFEDVYQL
60 70 80 90 100
TSELLGEGAY AKVQGAVNLQ SGKEYAVKII EKQAGHSRSR VFREVETLYQ
110 120 130 140 150
CQGNRNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKRK HFNEREASRV
160 170 180 190 200
VRDVATALDF LHTKGIAHRD LKPENILCES PEKVSPVKIC DFDLGSGVKL
210 220 230 240 250
NNSCTPITTP ELTTPCGSAE YMAPEVVEVF RDEATFYDKR CDLWSLGVVL
260 270 280 290 300
YIMLSGYPPF VGHCGADCGW DRGEVCRMCQ NKLFESIQEG KYEFPDKDWA
310 320 330 340 350
HISNEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPER GLPTPQVLQR
360 370 380 390 400
NSSTMDLTLF AAEAIALNRQ LSQHEENELA EEQEALAEGL CSMKLSPPSK
410 420
SRLARRRALA QAGRSRDANP CLTPAGL
Length:427
Mass (Da):47,915
Last modified:April 30, 2003 - v2
Checksum:i7D59A1CBEE4DF68F
GO

Sequence cautioni

The sequence AAH21369 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021369 mRNA Translation: AAH21369.1 Different initiation.
Y11091 mRNA Translation: CAA71965.1
RefSeqiNP_001272417.1, NM_001285488.1
NP_067436.1, NM_021461.5
UniGeneiMm.209327

Genome annotation databases

GeneIDi17346
KEGGimmu:17346
UCSCiuc008ufl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMKNK1_MOUSE
AccessioniPrimary (citable) accession number: O08605
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 23, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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