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Protein

Endonuclease G, mitochondrial

Gene

Endog

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).By similarity

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
Metal bindingi169 – 1691Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • apoptotic DNA fragmentation Source: MGI
  • cellular response to calcium ion Source: Ensembl
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to hypoxia Source: Ensembl
  • DNA catabolic process Source: UniProtKB
  • in utero embryonic development Source: MGI
  • neuron death in response to oxidative stress Source: Ensembl
  • positive regulation of apoptotic DNA fragmentation Source: Ensembl
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of hydrogen peroxide-mediated programmed cell death Source: Ensembl
  • response to antibiotic Source: MGI
  • response to estradiol Source: Ensembl
  • response to mechanical stimulus Source: Ensembl
  • response to tumor necrosis factor Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease G, mitochondrial (EC:3.1.30.-)
Short name:
Endo G
Gene namesi
Name:Endog
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1261433. Endog.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • mitochondrion Source: MGI
  • nucleus Source: Ensembl
  • perikaryon Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444MitochondrionBy similarityAdd
BLAST
Chaini45 – 294250Endonuclease G, mitochondrialPRO_0000019919Add
BLAST

Proteomic databases

EPDiO08600.
PaxDbiO08600.
PeptideAtlasiO08600.
PRIDEiO08600.

PTM databases

iPTMnetiO08600.
PhosphoSiteiO08600.

Expressioni

Gene expression databases

BgeeiO08600.
CleanExiMM_ENDOG.
ExpressionAtlasiO08600. baseline and differential.
GenevisibleiO08600. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO08600. 2 interactions.
MINTiMINT-1844836.
STRINGi10090.ENSMUSP00000015481.

Structurei

3D structure databases

ProteinModelPortaliO08600.
SMRiO08600. Positions 59-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3721. Eukaryota.
COG1864. LUCA.
HOGENOMiHOG000214172.
HOVERGENiHBG002851.
InParanoidiO08600.
KOiK01173.
OMAiHVFHRAT.
OrthoDBiEOG7VB2FZ.
PhylomeDBiO08600.
TreeFamiTF105386.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
InterProiIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamiPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALRAGLTL ALGAGLGAAA EHWRRREGKA PGLLGRVPLL PVVAADLPAL
60 70 80 90 100
PGGPAGGTGE LAKYGLPGVA QLRSRESYVL SYDPRTRGAL WVLEQLRPER
110 120 130 140 150
LRGDGDRSAC DFREDDSVHA YHRATNADYR GSGFDRGHLA AAANHRWSQR
160 170 180 190 200
AMDDTFYLSN VAPQVPHLNQ NAWNNLERYS RSLTRTYQNV YVCTGPLFLP
210 220 230 240 250
RTEADGKSYV KYQVIGKNHV AVPTHFFKVL ILEAAGGQIE LRSYVMPNAP
260 270 280 290
VDETIPLERF LVPIESIERA SGLLFVPNIL ARAGNLKAIT AGSK
Length:294
Mass (Da):32,191
Last modified:July 1, 1997 - v1
Checksum:i9F01FB1BC28B95D3
GO

Sequence cautioni

The sequence CAA67768.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99395 Genomic DNA. Translation: CAA67769.1.
X99394 mRNA. Translation: CAA67768.1. Different initiation.
AB012108 mRNA. Translation: BAA28168.1.
BC030177 mRNA. Translation: AAH30177.1.
CCDSiCCDS15872.1.
RefSeqiNP_031957.1. NM_007931.1.
UniGeneiMm.474473.

Genome annotation databases

EnsembliENSMUST00000015481; ENSMUSP00000015481; ENSMUSG00000015337.
GeneIDi13804.
KEGGimmu:13804.
UCSCiuc008jbk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99395 Genomic DNA. Translation: CAA67769.1.
X99394 mRNA. Translation: CAA67768.1. Different initiation.
AB012108 mRNA. Translation: BAA28168.1.
BC030177 mRNA. Translation: AAH30177.1.
CCDSiCCDS15872.1.
RefSeqiNP_031957.1. NM_007931.1.
UniGeneiMm.474473.

3D structure databases

ProteinModelPortaliO08600.
SMRiO08600. Positions 59-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08600. 2 interactions.
MINTiMINT-1844836.
STRINGi10090.ENSMUSP00000015481.

PTM databases

iPTMnetiO08600.
PhosphoSiteiO08600.

Proteomic databases

EPDiO08600.
PaxDbiO08600.
PeptideAtlasiO08600.
PRIDEiO08600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015481; ENSMUSP00000015481; ENSMUSG00000015337.
GeneIDi13804.
KEGGimmu:13804.
UCSCiuc008jbk.1. mouse.

Organism-specific databases

CTDi2021.
MGIiMGI:1261433. Endog.

Phylogenomic databases

eggNOGiKOG3721. Eukaryota.
COG1864. LUCA.
HOGENOMiHOG000214172.
HOVERGENiHBG002851.
InParanoidiO08600.
KOiK01173.
OMAiHVFHRAT.
OrthoDBiEOG7VB2FZ.
PhylomeDBiO08600.
TreeFamiTF105386.

Miscellaneous databases

ChiTaRSiEndog. mouse.
PROiO08600.
SOURCEiSearch...

Gene expression databases

BgeeiO08600.
CleanExiMM_ENDOG.
ExpressionAtlasiO08600. baseline and differential.
GenevisibleiO08600. MM.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
InterProiIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamiPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv and BALB/c.
    Tissue: Brain.
  2. Matsuda T.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6J.
    Tissue: Heart.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver and Spleen.

Entry informationi

Entry nameiNUCG_MOUSE
AccessioniPrimary (citable) accession number: O08600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.