O08599 (STXB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-binding protein 1

Alternative name(s):
Protein unc-18 homolog 1
Short name=Unc18-1
Protein unc-18 homolog A
Short name=Unc-18A

Gene names

Name:

Stxbp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions By similarity.
Subunit structure	Binds SYTL4 By similarity. Interacts with STX1A By similarity.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.
Sequence caution	The sequence CAM20726.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Transport
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axon target recognition Inferred from mutant phenotype PubMed 15182301. Source: MGI long term synaptic depression Inferred from mutant phenotype PubMed 17110441. Source: MGI negative regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 15255974. Source: MGI negative regulation of synaptic transmission, GABAergic Inferred from direct assay PubMed 17167098. Source: MGI neuromuscular synaptic transmission Inferred from mutant phenotype PubMed 17110441. Source: MGI neurotransmitter secretion Inferred from mutant phenotype PubMed 15255974. Source: MGI platelet aggregation Inferred from electronic annotation. Source: Compara platelet degranulation Inferred from electronic annotation. Source: Compara positive regulation of calcium ion-dependent exocytosis Inferred from direct assay PubMed 15123626. Source: MGI protein stabilization Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of synaptic vesicle priming Inferred from electronic annotation. Source: Compara synaptic vesicle maturation Inferred from mutant phenotype PubMed 15145078. Source: MGI vesicle docking involved in exocytosis Inferred from mutant phenotype PubMed 11545717. Source: MGI
Cellular_component	mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI plasma membrane Traceable author statement. Source: Reactome platelet alpha granule Inferred from electronic annotation. Source: Compara protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	syntaxin-1 binding Inferred from sequence orthology PubMed 7553862. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O08599-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O08599-2)

The sequence of this isoform differs from the canonical sequence as follows:
     576-594: QKLLDTLKKLNKTDEEISS → TKFLMDLRHPDFRESSRVSFEDQAPTME

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Syntaxin-binding protein 1		PRO_0000206278
Amino acid modifications
	Modified residue	107	1	Phosphothreonine Ref.7
	Modified residue	142	1	Phosphoserine Ref.7
	Modified residue	145	1	Phosphotyrosine Ref.7
	Modified residue	146	1	Phosphoserine Ref.7
	Modified residue	345	1	Phosphoserine Ref.7
	Modified residue	346	1	Phosphothreonine Ref.7
	Modified residue	473	1	Phosphotyrosine Ref.8
	Modified residue	507	1	Phosphoserine Ref.10
	Modified residue	509	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	576 – 594	19	QKLLD…EEISS → TKFLMDLRHPDFRESSRVSF EDQAPTME in isoform 2.		VSP_010496
	Natural variant	216	1	D → N in strain: DBA/2J.
Experimental info
	Sequence conflict	101	1	A → R in BAA19479. Ref.1
	Sequence conflict	367	1	R → H in BAA32486. Ref.2
	Sequence conflict	537	1	L → F in BAA19479. Ref.1
	Sequence conflict	561	1	G → A in BAA19479. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 2DD0715F875CE0F3	FASTA	594	67,569
	10 20 30 40 50 60 MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI MTEGITIVED 70 80 90 100 110 120 INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR AAHVFFTDSC PDALFNELVK 130 140 150 160 170 180 SRAAKVIKTL TEINIAFLPY ESQVYSLDSA DSFQSFYSPH KAQMKNPILE RLAEQIATLC 190 200 210 220 230 240 ATLKEYPAVR YRGEYKDNAL LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS 250 260 270 280 290 300 SPVLHELTFQ AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS 310 320 330 340 350 360 QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL AEDCMKHYQG 370 380 390 400 410 420 TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS TYDKIRIILL YIFLKNGITE 430 440 450 460 470 480 ENLNKLIQHA QIPPEDSEII TNMAHLGVPI VTDSTLRRRS KPERKERISE QTYQLSRWTP 490 500 510 520 530 540 IIKDIMEDTI EDKLDTKHYP YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF 550 560 570 580 590 ILGGVSLNEM RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
	Isoform 2 [UniParc]. Checksum: 73D8337768259493	FASTA	603	68,736
	10 20 30 40 50 60 MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI MTEGITIVED 70 80 90 100 110 120 INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR AAHVFFTDSC PDALFNELVK 130 140 150 160 170 180 SRAAKVIKTL TEINIAFLPY ESQVYSLDSA DSFQSFYSPH KAQMKNPILE RLAEQIATLC 190 200 210 220 230 240 ATLKEYPAVR YRGEYKDNAL LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS 250 260 270 280 290 300 SPVLHELTFQ AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS 310 320 330 340 350 360 QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL AEDCMKHYQG 370 380 390 400 410 420 TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS TYDKIRIILL YIFLKNGITE 430 440 450 460 470 480 ENLNKLIQHA QIPPEDSEII TNMAHLGVPI VTDSTLRRRS KPERKERISE QTYQLSRWTP 490 500 510 520 530 540 IIKDIMEDTI EDKLDTKHYP YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF 550 560 570 580 590 600 ILGGVSLNEM RCAYEVTQAN GKWEVLIGST HILTPTKFLM DLRHPDFRES SRVSFEDQAP TME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants." Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y. J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[2]	"Genomic structure of MUNC18-1 protein, which is involved in docking and fusion of synaptic vesicles in brain." Gotoh K., Yokota H., Kikuya E., Watanabe T., Oishi M. J. Biol. Chem. 273:21642-21647(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"Characterization of candidate genes for multiple ethanol influenced traits on mouse chromosome 2." Fehr C., Buck K.J. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J and DBA/2J. Tissue: Brain.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Eye.
[6]	Lubec G., Klug S., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 30-39; 47-98; 101-120; 162-184; 193-225; 228-235; 266-275; 278-314; 326-332; 344-356; 368-382; 389-425; 468-518 AND 537-594. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[7]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-107; SER-142; TYR-145; SER-146; SER-345 AND THR-346, MASS SPECTROMETRY. Tissue: Forebrain.
[8]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-473, MASS SPECTROMETRY. Tissue: Brain.
[9]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509, MASS SPECTROMETRY. Tissue: Brain cortex.
[10]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-507, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45903 mRNA. Translation: BAA19479.1. AB012697 Genomic DNA. Translation: BAA32486.1. AF326545 mRNA. Translation: AAL37391.1. AF326563 mRNA. Translation: AAL37409.1. AL845471 Genomic DNA. Translation: CAM20726.1. Sequence problems. AL845471 Genomic DNA. Translation: CAM20727.1. AL845471 Genomic DNA. Translation: CAM20728.1. BC031728 mRNA. Translation: AAH31728.1.
IPI	IPI00415402. IPI00415403.
RefSeq	NP_001107041.1. NM_001113569.1. NP_033321.2. NM_009295.2.
UniGene	Mm.278865.
3D structure databases
ProteinModelPortal	O08599.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31951N.
IntAct	O08599. 9 interactions.
STRING	10090.ENSMUSP00000089051.
PTM databases
PhosphoSite	O08599.
Proteomic databases
PaxDb	O08599.
PRIDE	O08599.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050000; ENSMUSP00000052440; ENSMUSG00000026797. ENSMUST00000077458; ENSMUSP00000089051; ENSMUSG00000026797. ENSMUST00000113222; ENSMUSP00000108848; ENSMUSG00000026797.
GeneID	20910.
KEGG	mmu:20910.
UCSC	uc008jha.2. mouse. uc008jhb.2. mouse.
Organism-specific databases
CTD	6812.
MGI	MGI:107363. Stxbp1.
Phylogenomic databases
eggNOG	COG5158.
GeneTree	ENSGT00390000005206.
HOGENOM	HOG000232146.
HOVERGEN	HBG052710.
KO	K15292.
OMA	PDPLFNE.
OrthoDB	EOG4K6G3W.
Enzyme and pathway databases
Reactome	REACT_112621. Metabolism.
Gene expression databases
Bgee	O08599.
CleanEx	MM_STXBP1.
Genevestigator	O08599.
GermOnline	ENSMUSG00000026797. Mus musculus.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
PANTHER	PTHR11679. PTHR11679. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
PIRSF	PIRSF005715. VPS45_Sec1. 1 hit.
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	STXBP1. mouse.
NextBio	299797.
SOURCE	Search...

Entry information

Entry name

STXB1_MOUSE

Accession

Primary (citable) accession number: O08599
Secondary accession number(s): A2ARS2 , A2ARS3, A2ARS4, Q5WAC6, Q8VD51

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 7, 2004
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program