O08593 (PDE7A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
EC=3.1.4.17

Alternative name(s):
Rolipram-insensitive phosphodiesterase type 7

Gene names

Name:

Pde7a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	426 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction By similarity.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Insensitive to all selective PDE inhibitors By similarity.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Subunit structure	Interacts with CBFA2T3 By similarity.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

Ontologies

Keywords
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cAMP-mediated signaling Inferred from expression pattern. Source: RGD
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Traceable author statement. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 426	›426	High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A		PRO_0000198835
Regions
	Region	131 – 395	265	Catalytic By similarity
Sites
	Active site	156	1	Proton donor By similarity
	Metal binding	160	1	Divalent metal cation 1 By similarity
	Metal binding	196	1	Divalent metal cation 1 By similarity
	Metal binding	197	1	Divalent metal cation 1 By similarity
	Metal binding	197	1	Divalent metal cation 2 By similarity
	Metal binding	306	1	Divalent metal cation 1 By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O08593 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 129BDC01C9351D26	FASTA	426	49,274
	10 20 30 40 50 60 DQTALYIRML GDVRVRSRAG FETERRGSHP YIDFRIFHAQ SEIEASVSAR NIRRLLSFQR 70 80 90 100 110 120 YLRSSRFFRG ATVCRSLNIL DEDYNGQAKC MLEKVGNWNF DIFLFDRLTN GNSLVSLTFH 130 140 150 160 170 180 LFSLHGLIEY FHLDMVKLRR FLVMIQEDYH SQNPYHNAVH AADVTQAMHC YLKEPKLANS 190 200 210 220 230 240 VTPWDILLSL IAAATHDLDH PGVNQPFLIK TNHYLATLYK NTSVLENHHW RSAVGLLRES 250 260 270 280 290 300 GLFSHLPLES RHEMEAQIGA LILATDISRQ NEYLSLFRSH LDKGDLHLDD GRHRHLVLQM 310 320 330 340 350 360 ALKCADICNP CRNWELSKQW SEKVTEEFFH QGDIEKKYHL GVSPLCDRQT ESIANIQIGF 370 380 390 400 410 420 MTYLQEPLFT EWARFSDTRL SQTMLGHVGL NKASWKGLQR QQPSSEDASA AFELNSQLLT QENRLS

References

[1]	"Differential distribution of rat PDE-7 mRNA in embryonic and adult rat brain." Hoffmann R., Abdel'Al S., Engels P. Cell Biochem. Biophys. 28:103-113(1998) [PubMed: 9515162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77880 mRNA. Translation: AAB51234.1.
IPI	IPI00372177.
UniGene	Rn.39142.
3D structure databases
ProteinModelPortal	O08593.
SMR	O08593. Positions 83-399.
ModBase	Search...
Protein-protein interaction databases
STRING	O08593.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	68391. Pde7a.
Phylogenomic databases
eggNOG	roNOG06712.
GeneTree	ENSGT00590000082915.
HOVERGEN	HBG053543.
InParanoid	O08593.
OrthoDB	EOG48KRBC.
Gene expression databases
ArrayExpress	O08593.
Genevestigator	O08593.
GermOnline	ENSRNOG00000013048. Rattus norvegicus.
Family and domain databases
InterPro	IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE7A_RAT

Accession

Primary (citable) accession number: O08593

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program