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Protein

High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A

Gene

Pde7a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction (By similarity).By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Insensitive to all selective PDE inhibitors.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 1561Proton donorBy similarity
Metal bindingi160 – 1601Divalent metal cation 1By similarity
Metal bindingi196 – 1961Divalent metal cation 1By similarity
Metal bindingi197 – 1971Divalent metal cation 1By similarity
Metal bindingi197 – 1971Divalent metal cation 2By similarity
Metal bindingi306 – 3061Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
  2. cyclic-nucleotide phosphodiesterase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
  2. cAMP-mediated signaling Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A (EC:3.1.4.53)
Alternative name(s):
Rolipram-insensitive phosphodiesterase type 7
Gene namesi
Name:Pde7a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68391. Pde7a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 426›426High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7APRO_0000198835Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriO08593.

Interactioni

Subunit structurei

Interacts with CBFA2T3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040981.

Structurei

3D structure databases

ProteinModelPortaliO08593.
SMRiO08593. Positions 83-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 395265CatalyticBy similarityAdd
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG300643.
HOGENOMiHOG000220881.
HOVERGENiHBG053543.
InParanoidiO08593.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

O08593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DQTALYIRML GDVRVRSRAG FETERRGSHP YIDFRIFHAQ SEIEASVSAR
60 70 80 90 100
NIRRLLSFQR YLRSSRFFRG ATVCRSLNIL DEDYNGQAKC MLEKVGNWNF
110 120 130 140 150
DIFLFDRLTN GNSLVSLTFH LFSLHGLIEY FHLDMVKLRR FLVMIQEDYH
160 170 180 190 200
SQNPYHNAVH AADVTQAMHC YLKEPKLANS VTPWDILLSL IAAATHDLDH
210 220 230 240 250
PGVNQPFLIK TNHYLATLYK NTSVLENHHW RSAVGLLRES GLFSHLPLES
260 270 280 290 300
RHEMEAQIGA LILATDISRQ NEYLSLFRSH LDKGDLHLDD GRHRHLVLQM
310 320 330 340 350
ALKCADICNP CRNWELSKQW SEKVTEEFFH QGDIEKKYHL GVSPLCDRQT
360 370 380 390 400
ESIANIQIGF MTYLQEPLFT EWARFSDTRL SQTMLGHVGL NKASWKGLQR
410 420
QQPSSEDASA AFELNSQLLT QENRLS
Length:426
Mass (Da):49,274
Last modified:July 1, 1997 - v1
Checksum:i129BDC01C9351D26
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77880 mRNA. Translation: AAB51234.1.
UniGeneiRn.39142.

Genome annotation databases

UCSCiRGD:68391. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77880 mRNA. Translation: AAB51234.1.
UniGeneiRn.39142.

3D structure databases

ProteinModelPortaliO08593.
SMRiO08593. Positions 83-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:68391. rat.

Organism-specific databases

RGDi68391. Pde7a.

Phylogenomic databases

eggNOGiNOG300643.
HOGENOMiHOG000220881.
HOVERGENiHBG053543.
InParanoidiO08593.

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Miscellaneous databases

PROiO08593.

Gene expression databases

GenevestigatoriO08593.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Differential distribution of rat PDE-7 mRNA in embryonic and adult rat brain."
    Hoffmann R., Abdel'Al S., Engels P.
    Cell Biochem. Biophys. 28:103-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPDE7A_RAT
AccessioniPrimary (citable) accession number: O08593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.