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Reviewed, UniProtKB/Swiss-Prot O08590 (AOC3_RAT)

Last modified June 16, 2009. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Membrane primary amine oxidase
    EC=1.4.3.21
Alternative name(s):
    Copper amine oxidase
    Semicarbazide-sensitive amine oxidase
      Short name=SSAO
    Vascular adhesion protein 1
      Short name=VAP-1
    VP97
Gene names
Name: Aoc3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cell adhesion protein that participate in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. Ref.1

Catalytic activity

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.

Cofactor

Binds 1 copper ion per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Contains 1 topaquinone per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein. Ref.3 Ref.4

Tissue specificity

Highly expressed in adipocytes, aorta and lung. Expressed at lower levels in heart, kidney, large intestine, liver, small intestine and stomach. Ref.1

Post-translational modification

Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.

N- and O-glycosylated By similarity.

Sequence similarities

Belongs to the copper/topaquinone oxidase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 763762Membrane primary amine oxidase
PRO_0000064105

Regions

Topological domain2 – 65Cytoplasmic Potential
Transmembrane7 – 2721Signal-anchor for type II membrane protein Potential
Topological domain28 – 763736Extracellular Potential

Sites

Active site3861Proton acceptor By similarity
Active site4711Schiff-base intermediate with substrate; via topaquinone By similarity
Metal binding5201Copper By similarity
Metal binding5221Copper By similarity
Metal binding5291Calcium 1 By similarity
Metal binding5301Calcium 1; via carbonyl oxygen By similarity
Metal binding5311Calcium 1 By similarity
Metal binding5721Calcium 2 By similarity
Metal binding6381Calcium 2 By similarity
Metal binding6631Calcium 2; via carbonyl oxygen By similarity
Metal binding6651Calcium 2 By similarity
Metal binding6671Calcium 2 By similarity
Metal binding6731Calcium 1 By similarity
Metal binding6741Calcium 1; via carbonyl oxygen By similarity
Metal binding6841Copper By similarity

Amino acid modifications

Modified residue47112',4',5'-topaquinone By similarity
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation2121O-linked (GalNAc...) By similarity
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential
Glycosylation6661N-linked (GlcNAc...) Potential
Disulfide bond198 ↔ 199 By similarity
Disulfide bond734 ↔ 741 By similarity
Disulfide bond748Interchain By similarity

Experimental info

Sequence conflict541P → S in AAC53189. Ref.3
Sequence conflict3291Q → R in BAD74047. Ref.1
Sequence conflict6451S → F in BAD74047. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
O08590-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 5FD739AF43F39039

FASTA76384,981
        10         20         30         40         50         60 
MTQKTTLVLL ALAVITIFAL VCVLLAGRSG DGGRLSQPLH CPSVLPSVQP QTHPGQSQPF 

        70         80         90        100        110        120 
ADLSPEELTA VMSFLIKHLG PGLVDAAQAR PSDNCVFSVE LQLPAKAAAL AHLDRGGPPP 

       130        140        150        160        170        180 
VREALAIIFF GGQPKPNVSE LVVGPLPHPS YMRDVTVERH GGPLPYYRRP VLTREYQDIQ 

       190        200        210        220        230        240 
EMIFHRELPQ ASGLLHHCCF YKRQGHNLLK MTTAPRGLQS GDRATWFGIY YNLSGAGFYP 

       250        260        270        280        290        300 
HPIGLELLVD HKALDPALWT IQKVFYQGRY YESLTQLEDM FEAGLVNVVL VPDNGTGGSW 

       310        320        330        340        350        360 
SLKSSVPPGR APPLQFHPEG PRFSVQGSQV RSSLWAFSFG LGAFSGPRIF DIRFQGERVA 

       370        380        390        400        410        420 
YEISVQEAIA LYGGNSPASM STCYMDGSFG IGKYSTPLTR GVDCPYLATY VDWHFLLESQ 

       430        440        450        460        470        480 
TPKTLRDAFC VFEQNQGLPL RRHHSDFYSH YFGGVVETVL VVRSVATLLN YDYVWDMVFH 

       490        500        510        520        530        540 
SNGAIEVKFH ATGYITSAFF FGAGEKFGNR VAEHTLGTVH THNAHFKVDL DVAGLKNWAW 

       550        560        570        580        590        600 
AEDLAFVPMN VPWQPEFQMQ RLQVTRKLLE TEEEAAFPLG NATPRYLYLA SNHSNKWGHR 

       610        620        630        640        650        660 
RGYRIQILSF AGKPLPQESP IEKAFTWGRY HLAVTQRKEE EPSSSSIYNQ NDPWTPTVDF 

       670        680        690        700        710        720 
TDFISNETIA GEDLVAWVTA GFLHIPHAED IPNTVTVGNG VGFFLRPYNF FDEDPSFYSP 

       730        740        750        760 
DSIYFRKDQD VTDCEVNSLA CLSQTANCVP DLPAFSHGGF TYK

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References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of rat semicarbazide-sensitive amine oxidase."
Ochiai Y., Itoh K., Sakurai E., Tanaka Y.
Biol. Pharm. Bull. 28:413-418(2005) [PubMed: 15744061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Aorta.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[3]"Membrane amine oxidase cloning and identification as a major protein in the adipocyte plasma membrane."
Morris N.J., Ducret A., Aebersold R., Ross S.A., Keller S.R., Lienhard G.E.
J. Biol. Chem. 272:9388-9392(1997) [PubMed: 9083076] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-322, PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Adipocyte.
[4]"The major integral membrane glycoprotein in adipocytes is a novel 200-kDa heterodimer."
Jochen A., Guven S., Hays J.
Mol. Membr. Biol. 12:277-281(1995) [PubMed: 8520629] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20, SUBCELLULAR LOCATION.
Tissue: Adipocyte.

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Cross-references

Sequence databases

AB195675 mRNA. Translation: BAD74047.1.
BC100613 mRNA. Translation: AAI00614.1.
U72632 mRNA. Translation: AAC53189.1.
IPIIPI00515821.
RefSeqNP_113770.2.
UniGeneRn.198327

3D structure databases

SMRO08590. Positions 58-716.
ModBaseSearch...

Genome annotation databases

GeneID29473.
KEGGrno:29473.

Organism-specific databases

RGD62058. Aoc3.

Phylogenomic databases

HOVERGENO08590.

Enzyme and pathway databases

BRENDA1.4.3.6. 248.

Gene expression databases

ArrayExpressO08590.

Family and domain databases

InterProIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR015800. Cu_amine_oxidase_N2.
IPR015801. Cu_amine_oxidase_N2/3.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
Gene3DG3DSA:3.10.450.40. CuNH_oxidase. 2 hits.
G3DSA:2.70.98.20. Lyase_8_central. 1 hit.
PANTHERPTHR10638. CuNH_oxidase. 1 hit.
PfamPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSPR00766. CUDAOXIDASE.
PROSITEPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio609300.

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Entry information

Entry nameAOC3_RAT
AccessionPrimary (citable) accession number: O08590
Secondary accession number(s): Q497D2, Q5R1T5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 82 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents