O08589 (PLM_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholemman

Alternative name(s):
FXYD domain-containing ion transport regulator 1

Gene names

Name:	Fxyd1
Synonyms:	Plm

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK By similarity.
Sequence similarities	Belongs to the FXYD family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ionic channel
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Inferred from direct assay. Source: RGD muscle contraction Inferred from direct assay. Source: RGD
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 92	72	Phospholemman		PRO_0000010361
Regions
	Topological domain	21 – 35	15	Extracellular Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Topological domain	57 – 92	36	Cytoplasmic Potential
Amino acid modifications
	Modified residue	79	1	Phosphothreonine By similarity
	Modified residue	83	1	Phosphoserine; by PKA and PKC By similarity
	Modified residue	88	1	Phosphoserine; by PKA By similarity
Experimental info
	Sequence conflict	3 – 5	3	SPG → PLH in AAC53169. Ref.1
	Sequence conflict	43 – 46	4	IAGI → AGIL in AAC53169. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O08589 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 29530D084B3CB7C2	FASTA	92	10,365
	10 20 30 40 50 60 MASPGHILIV CVCLLSMASA EAPQEPDPFT YDYHTLRIGG LTIAGILFIL GILIILSKRC 70 80 90 RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR

References

[1]	"Characterization of the human and rat phospholemman (PLM) cDNAs and localization of the human PLM gene to chromosome 19q13.1." Chen L.-S.K., Lo C.F., Numann R., Cuddy M. Genomics 41:435-443(1997) [PubMed: 9169143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression." Sweadner K.J., Rael E. Genomics 68:41-56(2000) [PubMed: 10950925] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 70-81, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72246 mRNA. Translation: AAC53169.1.
IPI	IPI00193828.
RefSeq	NP_113836.1. NM_031648.1.
UniGene	Rn.3828.
3D structure databases
ProteinModelPortal	O08589.
SMR	O08589. Positions 21-92.
ModBase	Search...
Protein-protein interaction databases
STRING	O08589.
PTM databases
PhosphoSite	O08589.
Proteomic databases
PRIDE	O08589.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028624; ENSRNOP00000028624; ENSRNOG00000021079.
GeneID	58971.
KEGG	rno:58971.
Organism-specific databases
CTD	5348.
RGD	69306. Fxyd1.
Phylogenomic databases
eggNOG	maNOG21518.
GeneTree	ENSGT00530000063932.
HOVERGEN	HBG008212.
InParanoid	O08589.
OMA	RKCRCKF.
OrthoDB	EOG4PG62F.
PhylomeDB	O08589.
Gene expression databases
ArrayExpress	O08589.
Genevestigator	O08589.
GermOnline	ENSRNOG00000021079. Rattus norvegicus.
Family and domain databases
InterPro	IPR000272. Ion-transport_regulator_FXYD. [Graphical view]
KO	K13360.
Pfam	PF02038. ATP1G1_PLM_MAT8. 1 hit. [Graphical view]
ProDom	PD005989. PD005989. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01310. FXYD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611640.

Entry information

Entry name

PLM_RAT

Accession

Primary (citable) accession number: O08589

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 30, 2002
Last modified:	December 14, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program