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Protein

Nuclear pore complex protein Nup50

Gene

Nup50

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:Nup50
Synonyms:Npap60
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3191. Nup50.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048701 – 467Nuclear pore complex protein Nup50Add BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei52PhosphoserineBy similarity1
Modified residuei83N6-acetyllysineBy similarity1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei247PhosphothreonineBy similarity1
Modified residuei269PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Cross-linki352Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei449N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO08587.

PTM databases

iPTMnetiO08587.
PhosphoSitePlusiO08587.

Expressioni

Tissue specificityi

Highly expressed in testis, intermediate levels in kidney, liver, spleen and low basal levels in somatic cells. Expression in testis undergoes changes and subcellular localization during germ cell differentiation.

Interactioni

Subunit structurei

Does not interact with TPR (By similarity). Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiO08587.
STRINGi10116.ENSRNOP00000018155.

Structurei

3D structure databases

ProteinModelPortaliO08587.
SMRiO08587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati113 – 11422
Repeati226 – 22732
Repeati272 – 27342
Repeati302 – 30352
Domaini334 – 467RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3035 X 2 AA repeats of F-GAdd BLAST228
Regioni144 – 206Binding to CDKN1BBy similarityAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 91Gly-richAdd BLAST37
Compositional biasi267 – 330Ser-richAdd BLAST64

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724. Eukaryota.
ENOG410YPHB. LUCA.
HOGENOMiHOG000007919.
HOVERGENiHBG052697.
InParanoidiO08587.
KOiK14295.
PhylomeDBiO08587.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR015007. NUP2/50/61.
IPR011993. PH-like_dom_sf.
IPR000156. Ran_bind_dom.
PfamiView protein in Pfam
PF08911. NUP50. 1 hit.
PF00638. Ran_BP1. 1 hit.
SMARTiView protein in SMART
SM00160. RanBD. 1 hit.
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50196. RANBD1. 1 hit.

Sequencei

Sequence statusi: Complete.

O08587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNIGF
60 70 80 90 100
ESDSGGAFKG FKGLVVPSGG GGFSGFGGGS GGKPLEGLTN GNSTDSATPF
110 120 130 140 150
SSAKTAAEPK AAFGSFAVNG PTTLVDKKIS SPKCNSSNQP PSSGPASSTS
160 170 180 190 200
CTGNTYHKQL AGLNCSVRDW IVKHVNTNPL CDLTPIFKDY ERYLATIEKQ
210 220 230 240 250
LENGGSSSSE RQTDRATAAM EPPSLFGSTK LQQDSPFSFH GNKAEDTSEK
260 270 280 290 300
LEFTAEKKSD AAQGATSASF NFGKKIESSV LGSLSSGSLT GFSFSPGNSS
310 320 330 340 350
LFGKDAAQSK AASSPFSAKA SESQAGGSSS ECRDGEEEES DEPPKVVVTE
360 370 380 390 400
VKEEDAFYSK KCKLFYKKDN EFKEKGVGTL HLKPTATQKT QLLVRADTNL
410 420 430 440 450
GNILLNVLIP PNMPCTRTGK NNVLIVCVPN PPLDEKQPTL PVTMLIRVKT
460
SEDADELHKI LLQKKDV
Length:467
Mass (Da):49,818
Last modified:May 1, 2000 - v2
Checksum:iCF213296F37F0884
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211R → S in AAH97337 (PubMed:15489334).Curated1
Sequence conflicti346V → A in AAH97337 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41845 mRNA. Translation: AAC53278.2.
BC097337 mRNA. Translation: AAH97337.1.
RefSeqiNP_037123.1. NM_012991.1.
UniGeneiRn.3242.

Genome annotation databases

GeneIDi25497.
KEGGirno:25497.

Similar proteinsi

Entry informationi

Entry nameiNUP50_RAT
AccessioniPrimary (citable) accession number: O08587
Secondary accession number(s): Q4QR93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome