Potassium channel subfamily K member 1

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O08581 (KCNK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 1

Alternative name(s):
Inward rectifying potassium channel protein TWIK-1

Gene names

Name:

Kcnk1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weak inwardly rectifying potassium channel.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Widely expressed. Highest expression in brain, kidney, thyroid, salivary gland, adrenal gland, prostate, epididymis, uterus, placenta, colon and jejunum. Moderate expression in eyes, pituitary, pancreas, smooth muscle, testis and ovary. Very low levels in lung, aorta, liver, heart, skeletal muscle, thymus and spleen. In the brain, highest expression in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.
Developmental stage	Expression detected as early as 7 days post conception. Expression increases from 2-8 days after birth and stabilizes after day 8.
Miscellaneous	Inhibited by quinine, barium, and internal acidification. Activated by protein kinase C.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to nicotine Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara brush border membrane Inferred from electronic annotation. Source: Compara endosome Inferred from direct assay PubMed 15540117. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	potassium channel activity Inferred from direct assay PubMed 15540117. Source: MGI voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 336

336

Potassium channel subfamily K member 1

PRO_0000101741

Regions

Topological domain

1 – 20

Cytoplasmic Potential

Transmembrane

21 – 41

Helical; Potential

Intramembrane

104 – 130

Pore-forming; Name=Pore-forming 1; Potential

Transmembrane

133 – 153

Helical; Potential

Topological domain

154 – 177

Cytoplasmic Potential

Transmembrane

178 – 198

Helical; Potential

Intramembrane

212 – 238

Pore-forming; Name=Pore-forming 2; Potential

Transmembrane

247 – 267

Helical; Potential

Topological domain

268 – 336

Cytoplasmic Potential

Amino acid modifications

Modified residue

326

Phosphoserine Ref.5

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

Interchain By similarity

Experimental info

Sequence conflict

V → E in AAC16973. Ref.1

Sequence conflict

152

V → L in AAC16973. Ref.1

Sequence conflict

259

A → T in AAC16973. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O08581 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 76B7FD5361A6216C
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FASTA

336

38,201

        10         20         30         40         50         60 
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 

        70         80         90        100        110        120 
RRFLEEHECL SEPQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 

       130        140        150        160        170        180 
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRVTVHV TRRPVLYFHI RWGFSKQVVA 

       190        200        210        220        230        240 
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 

       250        260        270        280        290        300 
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDL VHIMEHDQLS 

       310        320        330 
FSSVTEQVAG LKEEQKQSEP FVASQSPPYE DGSADH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structure, function and distribution of the mouse TWIK-1 K+ channel." Lesage F., Lauritzen I., Duprat F., Reyes R., Fink M., Heurteaux C., Lazdunski M. FEBS Lett. 402:28-32(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene." Arrighi I., Lesage F., Scimeca J.-C., Carle G.F., Barhanin J. FEBS Lett. 425:310-316(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvJ. Tissue: Liver.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF033017 mRNA. Translation: AAC16973.1. CH466525 Genomic DNA. Translation: EDL11808.1. BC003729 mRNA. Translation: AAH03729.1.
IPI	IPI00114405.
RefSeq	NP_032456.2. NM_008430.2.
UniGene	Mm.10800.
3D structure databases
ProteinModelPortal	O08581.
SMR	O08581. Positions 19-281.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000046103.
Protein family/group databases
TCDB	1.A.1.8.1. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	O08581.
Proteomic databases
PRIDE	O08581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000046765; ENSMUSP00000046103; ENSMUSG00000033998.
GeneID	16525.
KEGG	mmu:16525.
Organism-specific databases
CTD	3775.
MGI	MGI:109322. Kcnk1.
Phylogenomic databases
eggNOG	NOG290832.
GeneTree	ENSGT00700000104448.
HOGENOM	HOG000286014.
HOVERGEN	HBG052237.
InParanoid	Q99L99.
KO	K04912.
OMA	KQLRKMF.
OrthoDB	EOG451DRF.
Gene expression databases
Bgee	O08581.
CleanEx	MM_KCNK1.
Genevestigator	O08581.
GermOnline	ENSMUSG00000033998. Mus musculus.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR022306. 2pore_dom_K_chnl_TASK/TWIK. IPR005408. 2pore_dom_K_chnl_TWIK. IPR001779. 2pore_dom_K_chnl_TWIK1. IPR013099. Ion_trans_2. [Graphical view]
PANTHER	PTHR11003:SF27. PTHR11003:SF27. 1 hit.
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PIRSF	PIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTS	PR01333. 2POREKCHANEL. PR01096. TWIK1CHANNEL. PR01586. TWIKCHANNEL.
ProtoNet	Search...
Other
NextBio	289919.
SOURCE	Search...

Entry information

Entry name

KCNK1_MOUSE

Accession

Primary (citable) accession number: O08581
Secondary accession number(s): Q99L99

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents