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O08581 (KCNK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel subfamily K member 1
Alternative name(s):
Inward rectifying potassium channel protein TWIK-1
Gene names
Name:Kcnk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Weak inwardly rectifying potassium channel.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Widely expressed. Highest expression in brain, kidney, thyroid, salivary gland, adrenal gland, prostate, epididymis, uterus, placenta, colon and jejunum. Moderate expression in eyes, pituitary, pancreas, smooth muscle, testis and ovary. Very low levels in lung, aorta, liver, heart, skeletal muscle, thymus and spleen. In the brain, highest expression in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.

Developmental stage

Expression detected as early as 7 days post conception. Expression increases from 2-8 days after birth and stabilizes after day 8.

Miscellaneous

Inhibited by quinine, barium, and internal acidification. Activated by protein kinase C.

Sequence similarities

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Potassium channel subfamily K member 1
PRO_0000101741

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Intramembrane104 – 13027Pore-forming; Name=Pore-forming 1; Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 17724Cytoplasmic Potential
Transmembrane178 – 19821Helical; Potential
Intramembrane212 – 23827Pore-forming; Name=Pore-forming 2; Potential
Transmembrane247 – 26721Helical; Potential
Topological domain268 – 33669Cytoplasmic Potential

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Disulfide bond69Interchain By similarity

Experimental info

Sequence conflict441V → E in AAC16973. Ref.1
Sequence conflict1521V → L in AAC16973. Ref.1
Sequence conflict2591A → T in AAC16973. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O08581 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 76B7FD5361A6216C

FASTA33638,201
        10         20         30         40         50         60 
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 

        70         80         90        100        110        120 
RRFLEEHECL SEPQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 

       130        140        150        160        170        180 
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRVTVHV TRRPVLYFHI RWGFSKQVVA 

       190        200        210        220        230        240 
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 

       250        260        270        280        290        300 
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDL VHIMEHDQLS 

       310        320        330 
FSSVTEQVAG LKEEQKQSEP FVASQSPPYE DGSADH 

« Hide

References

« Hide 'large scale' references
[1]"The structure, function and distribution of the mouse TWIK-1 K+ channel."
Lesage F., Lauritzen I., Duprat F., Reyes R., Fink M., Heurteaux C., Lazdunski M.
FEBS Lett. 402:28-32(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene."
Arrighi I., Lesage F., Scimeca J.-C., Carle G.F., Barhanin J.
FEBS Lett. 425:310-316(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/SvJ.
Tissue: Liver.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF033017 mRNA. Translation: AAC16973.1.
CH466525 Genomic DNA. Translation: EDL11808.1.
BC003729 mRNA. Translation: AAH03729.1.
CCDSCCDS22783.1.
RefSeqNP_032456.2. NM_008430.2.
UniGeneMm.10800.

3D structure databases

ProteinModelPortalO08581.
SMRO08581. Positions 19-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200907. 2 interactions.
STRING10090.ENSMUSP00000046103.

Protein family/group databases

TCDB1.A.1.8.1. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteO08581.

Proteomic databases

PRIDEO08581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000046765; ENSMUSP00000046103; ENSMUSG00000033998.
GeneID16525.
KEGGmmu:16525.
UCSCuc009nyr.3. mouse.

Organism-specific databases

CTD3775.
MGIMGI:109322. Kcnk1.

Phylogenomic databases

eggNOGNOG290832.
GeneTreeENSGT00730000110802.
HOGENOMHOG000286014.
HOVERGENHBG052237.
InParanoidQ99L99.
KOK04912.
OMAKQLRKMF.
OrthoDBEOG7TTQ85.
TreeFamTF313947.

Gene expression databases

BgeeO08581.
CleanExMM_KCNK1.
GenevestigatorO08581.

Family and domain databases

InterProIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR001779. 2pore_dom_K_chnl_TWIK1.
[Graphical view]
PfamPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSPR01333. 2POREKCHANEL.
PR01096. TWIK1CHANNEL.
PR01586. TWIKCHANNEL.
ProtoNetSearch...

Other

NextBio289919.
PROO08581.
SOURCESearch...

Entry information

Entry nameKCNK1_MOUSE
AccessionPrimary (citable) accession number: O08581
Secondary accession number(s): Q99L99
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot