Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O08581

- KCNK1_MOUSE

UniProt

O08581 - KCNK1_MOUSE

Protein

Potassium channel subfamily K member 1

Gene

Kcnk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Weak inwardly rectifying potassium channel.

    GO - Molecular functioni

    1. potassium channel activity Source: MGI
    2. voltage-gated ion channel activity Source: UniProtKB-KW

    GO - Biological processi

    1. potassium ion transport Source: MGI
    2. response to nicotine Source: Ensembl

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199065. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

    Protein family/group databases

    TCDBi1.A.1.8.1. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel subfamily K member 1
    Alternative name(s):
    Inward rectifying potassium channel protein TWIK-1
    Gene namesi
    Name:Kcnk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:109322. Kcnk1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. brush border membrane Source: Ensembl
    3. endosome Source: MGI
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Potassium channel subfamily K member 1PRO_0000101741Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi69 – 69InterchainBy similarity
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO08581.

    PTM databases

    PhosphoSiteiO08581.

    Expressioni

    Tissue specificityi

    Widely expressed. Highest expression in brain, kidney, thyroid, salivary gland, adrenal gland, prostate, epididymis, uterus, placenta, colon and jejunum. Moderate expression in eyes, pituitary, pancreas, smooth muscle, testis and ovary. Very low levels in lung, aorta, liver, heart, skeletal muscle, thymus and spleen. In the brain, highest expression in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.

    Developmental stagei

    Expression detected as early as 7 days post conception. Expression increases from 2-8 days after birth and stabilizes after day 8.

    Gene expression databases

    BgeeiO08581.
    CleanExiMM_KCNK1.
    GenevestigatoriO08581.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    BioGridi200907. 2 interactions.
    STRINGi10090.ENSMUSP00000046103.

    Structurei

    3D structure databases

    ProteinModelPortaliO08581.
    SMRiO08581. Positions 19-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini154 – 17724CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini268 – 33669CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei104 – 13027Pore-forming; Name=Pore-forming 1Sequence AnalysisAdd
    BLAST
    Intramembranei212 – 23827Pore-forming; Name=Pore-forming 2Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei247 – 26721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290832.
    GeneTreeiENSGT00730000110802.
    HOGENOMiHOG000286014.
    HOVERGENiHBG052237.
    InParanoidiQ99L99.
    KOiK04912.
    OMAiKQLRKMF.
    OrthoDBiEOG7TTQ85.
    TreeFamiTF313947.

    Family and domain databases

    InterProiIPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR003092. 2pore_dom_K_chnl_TASK.
    IPR005408. 2pore_dom_K_chnl_TWIK.
    IPR001779. 2pore_dom_K_chnl_TWIK1.
    [Graphical view]
    PfamiPF07885. Ion_trans_2. 2 hits.
    [Graphical view]
    PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
    PRINTSiPR01333. 2POREKCHANEL.
    PR01096. TWIK1CHANNEL.
    PR01586. TWIKCHANNEL.

    Sequencei

    Sequence statusi: Complete.

    O08581-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED    50
    LLRQELRKLK RRFLEEHECL SEPQLEQFLG RVLEASNYGV SVLSNASGNW 100
    NWDFTSALFF ASTVLSTTGY GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT 150
    AVVQRVTVHV TRRPVLYFHI RWGFSKQVVA IVHAVLLGFV TVSCFFFIPA 200
    AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK FRELYKIGIT 250
    CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDL VHIMEHDQLS 300
    FSSVTEQVAG LKEEQKQSEP FVASQSPPYE DGSADH 336
    Length:336
    Mass (Da):38,201
    Last modified:July 27, 2011 - v2
    Checksum:i76B7FD5361A6216C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441V → E in AAC16973. (PubMed:9013852)Curated
    Sequence conflicti152 – 1521V → L in AAC16973. (PubMed:9013852)Curated
    Sequence conflicti259 – 2591A → T in AAC16973. (PubMed:9013852)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033017 mRNA. Translation: AAC16973.1.
    CH466525 Genomic DNA. Translation: EDL11808.1.
    BC003729 mRNA. Translation: AAH03729.1.
    CCDSiCCDS22783.1.
    RefSeqiNP_032456.2. NM_008430.2.
    UniGeneiMm.10800.

    Genome annotation databases

    EnsembliENSMUST00000046765; ENSMUSP00000046103; ENSMUSG00000033998.
    GeneIDi16525.
    KEGGimmu:16525.
    UCSCiuc009nyr.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033017 mRNA. Translation: AAC16973.1 .
    CH466525 Genomic DNA. Translation: EDL11808.1 .
    BC003729 mRNA. Translation: AAH03729.1 .
    CCDSi CCDS22783.1.
    RefSeqi NP_032456.2. NM_008430.2.
    UniGenei Mm.10800.

    3D structure databases

    ProteinModelPortali O08581.
    SMRi O08581. Positions 19-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200907. 2 interactions.
    STRINGi 10090.ENSMUSP00000046103.

    Protein family/group databases

    TCDBi 1.A.1.8.1. the voltage-gated ion channel (vic) superfamily.

    PTM databases

    PhosphoSitei O08581.

    Proteomic databases

    PRIDEi O08581.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046765 ; ENSMUSP00000046103 ; ENSMUSG00000033998 .
    GeneIDi 16525.
    KEGGi mmu:16525.
    UCSCi uc009nyr.3. mouse.

    Organism-specific databases

    CTDi 3775.
    MGIi MGI:109322. Kcnk1.

    Phylogenomic databases

    eggNOGi NOG290832.
    GeneTreei ENSGT00730000110802.
    HOGENOMi HOG000286014.
    HOVERGENi HBG052237.
    InParanoidi Q99L99.
    KOi K04912.
    OMAi KQLRKMF.
    OrthoDBi EOG7TTQ85.
    TreeFami TF313947.

    Enzyme and pathway databases

    Reactomei REACT_199065. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

    Miscellaneous databases

    NextBioi 289919.
    PROi O08581.
    SOURCEi Search...

    Gene expression databases

    Bgeei O08581.
    CleanExi MM_KCNK1.
    Genevestigatori O08581.

    Family and domain databases

    InterProi IPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR003092. 2pore_dom_K_chnl_TASK.
    IPR005408. 2pore_dom_K_chnl_TWIK.
    IPR001779. 2pore_dom_K_chnl_TWIK1.
    [Graphical view ]
    Pfami PF07885. Ion_trans_2. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF038061. K_channel_subfamily_K_type. 1 hit.
    PRINTSi PR01333. 2POREKCHANEL.
    PR01096. TWIK1CHANNEL.
    PR01586. TWIKCHANNEL.
    ProtoNeti Search...

    Publicationsi

    1. "The structure, function and distribution of the mouse TWIK-1 K+ channel."
      Lesage F., Lauritzen I., Duprat F., Reyes R., Fink M., Heurteaux C., Lazdunski M.
      FEBS Lett. 402:28-32(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene."
      Arrighi I., Lesage F., Scimeca J.-C., Carle G.F., Barhanin J.
      FEBS Lett. 425:310-316(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/SvJ.
      Tissue: Liver.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiKCNK1_MOUSE
    AccessioniPrimary (citable) accession number: O08581
    Secondary accession number(s): Q99L99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Inhibited by quinine, barium, and internal acidification. Activated by protein kinase C.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3