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Protein

Potassium channel subfamily K member 1

Gene

Kcnk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly inward rectifying voltage-gated potassium channel.1 Publication

Enzyme regulationi

Inhibited by quinine, barium, and internal acidification. Activated by protein kinase C.1 Publication

GO - Molecular functioni

  • inward rectifier potassium channel activity Source: UniProtKB
  • potassium channel activity Source: MGI
  • potassium ion leak channel activity Source: GO_Central

GO - Biological processi

  • potassium ion transmembrane transport Source: UniProtKB
  • potassium ion transport Source: MGI
  • response to nicotine Source: Ensembl
  • stabilization of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_320130. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

Protein family/group databases

TCDBi1.A.1.8.1. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 1
Alternative name(s):
Inward rectifying potassium channel protein TWIK-1
Gene namesi
Name:Kcnk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:109322. Kcnk1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicCuratedAdd
BLAST
Transmembranei21 – 4121HelicalBy similarityAdd
BLAST
Topological domaini42 – 10362ExtracellularCuratedAdd
BLAST
Intramembranei104 – 11613Helical; Name=Pore helix 1By similarityAdd
BLAST
Intramembranei117 – 1226By similarity
Topological domaini123 – 13210ExtracellularCurated
Transmembranei133 – 15624HelicalBy similarityAdd
BLAST
Topological domaini157 – 18125CytoplasmicCuratedAdd
BLAST
Transmembranei182 – 20221HelicalBy similarityAdd
BLAST
Topological domaini203 – 2119ExtracellularCurated
Intramembranei212 – 22413Helical; Name=Pore helix 2By similarityAdd
BLAST
Intramembranei225 – 2317By similarity
Topological domaini232 – 24312ExtracellularCuratedAdd
BLAST
Transmembranei244 – 26724HelicalBy similarityAdd
BLAST
Topological domaini268 – 33669CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • brush border membrane Source: Ensembl
  • endosome Source: MGI
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • inward rectifier potassium channel complex Source: UniProtKB
  • potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Potassium channel subfamily K member 1PRO_0000101741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi69 – 69InterchainBy similarity
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08581.
PRIDEiO08581.

PTM databases

PhosphoSiteiO08581.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in brain, kidney, thyroid, salivary gland, adrenal gland, prostate, epididymis, uterus, placenta, colon and jejunum. Moderate expression in eyes, pituitary, pancreas, smooth muscle, testis and ovary. Very low levels in lung, aorta, liver, heart, skeletal muscle, thymus and spleen. In the brain, highest expression in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.2 Publications

Developmental stagei

Expression detected as early as 7 days post conception. Expression increases from 2-8 days after birth and stabilizes after day 8.1 Publication

Gene expression databases

BgeeiO08581.
CleanExiMM_KCNK1.
GenevisibleiO08581. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

BioGridi200907. 2 interactions.
STRINGi10090.ENSMUSP00000046103.

Structurei

3D structure databases

ProteinModelPortaliO08581.
SMRiO08581. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1226Selectivity filter 1By similarity
Regioni225 – 2306Selectivity filter 2By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290832.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000286014.
HOVERGENiHBG052237.
InParanoidiO08581.
KOiK04912.
OMAiKQLRKMF.
OrthoDBiEOG7TTQ85.
TreeFamiTF313947.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR001779. 2pore_dom_K_chnl_TWIK1.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSiPR01333. 2POREKCHANEL.
PR01096. TWIK1CHANNEL.
PR01586. TWIKCHANNEL.

Sequencei

Sequence statusi: Complete.

O08581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED
60 70 80 90 100
LLRQELRKLK RRFLEEHECL SEPQLEQFLG RVLEASNYGV SVLSNASGNW
110 120 130 140 150
NWDFTSALFF ASTVLSTTGY GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT
160 170 180 190 200
AVVQRVTVHV TRRPVLYFHI RWGFSKQVVA IVHAVLLGFV TVSCFFFIPA
210 220 230 240 250
AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK FRELYKIGIT
260 270 280 290 300
CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDL VHIMEHDQLS
310 320 330
FSSVTEQVAG LKEEQKQSEP FVASQSPPYE DGSADH
Length:336
Mass (Da):38,201
Last modified:July 27, 2011 - v2
Checksum:i76B7FD5361A6216C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441V → E in AAC16973 (PubMed:9013852).Curated
Sequence conflicti152 – 1521V → L in AAC16973 (PubMed:9013852).Curated
Sequence conflicti259 – 2591A → T in AAC16973 (PubMed:9013852).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033017 mRNA. Translation: AAC16973.1.
CH466525 Genomic DNA. Translation: EDL11808.1.
BC003729 mRNA. Translation: AAH03729.1.
CCDSiCCDS22783.1.
RefSeqiNP_032456.2. NM_008430.2.
UniGeneiMm.10800.

Genome annotation databases

EnsembliENSMUST00000046765; ENSMUSP00000046103; ENSMUSG00000033998.
GeneIDi16525.
KEGGimmu:16525.
UCSCiuc009nyr.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033017 mRNA. Translation: AAC16973.1.
CH466525 Genomic DNA. Translation: EDL11808.1.
BC003729 mRNA. Translation: AAH03729.1.
CCDSiCCDS22783.1.
RefSeqiNP_032456.2. NM_008430.2.
UniGeneiMm.10800.

3D structure databases

ProteinModelPortaliO08581.
SMRiO08581. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200907. 2 interactions.
STRINGi10090.ENSMUSP00000046103.

Protein family/group databases

TCDBi1.A.1.8.1. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiO08581.

Proteomic databases

MaxQBiO08581.
PRIDEiO08581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046765; ENSMUSP00000046103; ENSMUSG00000033998.
GeneIDi16525.
KEGGimmu:16525.
UCSCiuc009nyr.3. mouse.

Organism-specific databases

CTDi3775.
MGIiMGI:109322. Kcnk1.

Phylogenomic databases

eggNOGiNOG290832.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000286014.
HOVERGENiHBG052237.
InParanoidiO08581.
KOiK04912.
OMAiKQLRKMF.
OrthoDBiEOG7TTQ85.
TreeFamiTF313947.

Enzyme and pathway databases

ReactomeiREACT_320130. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

Miscellaneous databases

ChiTaRSiKcnk1. mouse.
NextBioi289919.
PROiO08581.
SOURCEiSearch...

Gene expression databases

BgeeiO08581.
CleanExiMM_KCNK1.
GenevisibleiO08581. MM.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR001779. 2pore_dom_K_chnl_TWIK1.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSiPR01333. 2POREKCHANEL.
PR01096. TWIK1CHANNEL.
PR01586. TWIKCHANNEL.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The structure, function and distribution of the mouse TWIK-1 K+ channel."
    Lesage F., Lauritzen I., Duprat F., Reyes R., Fink M., Heurteaux C., Lazdunski M.
    FEBS Lett. 402:28-32(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Structure, chromosome localization, and tissue distribution of the mouse twik K+ channel gene."
    Arrighi I., Lesage F., Scimeca J.-C., Carle G.F., Barhanin J.
    FEBS Lett. 425:310-316(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: 129/SvJ.
    Tissue: Liver.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiKCNK1_MOUSE
AccessioniPrimary (citable) accession number: O08581
Secondary accession number(s): Q99L99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.