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O08579 (EMD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Emerin
Gene names
Name:Emd
Synonyms:Sta
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C By similarity.

Subunit structure

Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the inner nuclear membrane. Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin By similarity. Interacts with SUN1 and SUN2. Ref.7 Ref.9

Subcellular location

Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus By similarity. Ref.6 Ref.7

Tissue specificity

Detected in embryonic fibroblasts, skeletal muscle, heart muscle and tongue epithelium (at protein level). Widely expressed. Ref.1 Ref.6

Sequence similarities

Contains 1 LEM domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259Emerin
PRO_0000206141

Regions

Transmembrane224 – 24421Helical; Potential
Domain1 – 4545LEM
Region46 – 223178Interaction with F-actin By similarity
Region168 – 18720Interaction with CTNNB1 By similarity
Compositional bias53 – 597Poly-Ser
Compositional bias186 – 20015Poly-Ser

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue81Phosphoserine By similarity
Modified residue541Phosphoserine By similarity
Modified residue881Phosphoserine By similarity
Modified residue1611Phosphotyrosine Ref.8
Modified residue1711Phosphoserine By similarity

Experimental info

Sequence conflict961Y → C in BAE39870. Ref.3
Sequence conflict2541P → H in BAE39870. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O08579 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 645B021541063502

FASTA25929,436
        10         20         30         40         50         60 
MDDYAVLSDT ELAAVLRQYN IPHGPIVGST RKLYEKKIFE YETQRRRLLP PNSSSSSFSY 

        70         80         90        100        110        120 
QFSDLDSAAV DSDMYDLPKK EDALLYQSKD YNDDYYEESY LTTKTYGEPE SVGMSKSFRQ 

       130        140        150        160        170        180 
PGTSLVDADT FHHQVRDDIF SSLEEEGKDR ERLIYGQDSA YQSIAHYRPI SNVSRSSLGL 

       190        200        210        220        230        240 
SYYPTSSTSS VSSSSSSPSS WLTRRAIRPE KQAPAAALGQ DRQVPLWGQL LLFLVFAAFL 

       250 
LFVYYSIQAE EGNPFWMDP 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the complete mouse emerin gene."
Small K., Wagener M., Warren S.T.
Mamm. Genome 8:337-341(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: 129.
[2]"cDNA sequence and analysis of the murine Emery-Dreifuss muscular dystrophy gene."
Hawkes S.L.J., Neville L.A., Kennedy M.A.K., Love D.R.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: DBA/2.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy."
Sullivan T., Escalante-Alcalde D., Bhatt H., Anver M., Bhat N., Nagashima K., Stewart C.L., Burke B.
J. Cell Biol. 147:913-920(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"LUMA interacts with emerin and influences its distribution at the inner nuclear membrane."
Bengtsson L., Otto H.
J. Cell Sci. 121:536-548(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TMEM43, SUBCELLULAR LOCATION.
[8]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[9]"Mammalian SUN protein interaction networks at the inner nuclear membrane and their role in laminopathy disease processes."
Haque F., Mazzeo D., Patel J.T., Smallwood D.T., Ellis J.A., Shanahan C.M., Shackleton S.
J. Biol. Chem. 285:3487-3498(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SUN1 AND SUN2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U79753 Genomic DNA. Translation: AAB51239.1.
U73902 mRNA. Translation: AAD00238.1.
AK146361 mRNA. Translation: BAE27112.1.
AK167852 mRNA. Translation: BAE39870.1.
CH466650 Genomic DNA. Translation: EDL29837.1.
BC132131 mRNA. Translation: AAI32132.1.
BC132133 mRNA. Translation: AAI32134.1.
RefSeqNP_031953.1. NM_007927.3.
UniGeneMm.18892.

3D structure databases

ProteinModelPortalO08579.
SMRO08579. Positions 2-47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199436. 3 interactions.
IntActO08579. 2 interactions.
MINTMINT-4094379.
STRING10090.ENSMUSP00000002029.

PTM databases

PhosphoSiteO08579.

Proteomic databases

PaxDbO08579.
PRIDEO08579.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002029; ENSMUSP00000002029; ENSMUSG00000001964.
GeneID13726.
KEGGmmu:13726.
UCSCuc009toa.1. mouse.

Organism-specific databases

CTD2010.
MGIMGI:108117. Emd.

Phylogenomic databases

eggNOGNOG46326.
GeneTreeENSGT00390000002034.
HOGENOMHOG000081509.
HOVERGENHBG001099.
InParanoidO08579.
KOK12569.
OMASIAHYRP.
OrthoDBEOG7DFXDP.
PhylomeDBO08579.
TreeFamTF337236.

Gene expression databases

ArrayExpressO08579.
BgeeO08579.
CleanExMM_EMD.
GenevestigatorO08579.

Family and domain databases

Gene3D1.10.720.40. 1 hit.
InterProIPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamPF03020. LEM. 1 hit.
[Graphical view]
SMARTSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMSSF63451. SSF63451. 1 hit.
PROSITEPS50954. LEM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEMD. mouse.
NextBio284512.
PMAP-CutDBO08579.
PROO08579.
SOURCESearch...

Entry information

Entry nameEMD_MOUSE
AccessionPrimary (citable) accession number: O08579
Secondary accession number(s): Q3TIH6, Q3UJP3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot