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Protein

Mesoderm posterior protein 2

Gene

Mesp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.10 Publications

GO - Molecular functioni

  • DNA binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • mesodermal cell migration Source: MGI
  • Notch signaling pathway Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • signal transduction involved in regulation of gene expression Source: MGI
  • somite rostral/caudal axis specification Source: MGI
  • somitogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mesoderm posterior protein 2
Gene namesi
Name:Mesp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1096325. Mesp2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation2 Publications

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die shortly after birth and show caudal truncation and severe skeletal malformations. Lack of segmentation and impaired segment polarity of the paraxial mesoderm are the primarly defects. Mutants show altered expression of MEOX1, Pax1, and DLL1 and lack of expression of Notch1, Notch2, and FGFR1. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major defect is the apparent lack of any mesodermal layer between endoderm and ectoderm and a defect in the migratory activity of mesodermal cells.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi70 – 734RRTR → AATA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 145-A--A-150. 1 Publication
Mutagenesisi145 – 1506RRRRRR → AAAAAA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 70-A--A-73. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Mesoderm posterior protein 2PRO_0000296302Add
BLAST

Post-translational modificationi

Degraded by the proteasome.
Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO08574.

PTM databases

iPTMnetiO08574.
PhosphoSiteiO08574.

Expressioni

Developmental stagei

First transiently detected in the nascent mesoderm at the onset of gastrulation. A second site of expression is detected at 8 dpc in the rostral region of the presomitic mesoderm inmediately before segmentation. Down-regulated immediately after the formation of the segmented somites before 13.5 dpc. Initially expressed throughout the length of one somite, and then quickly repressed in the presumptive caudal region. Mesp2 is involved in the rapid down-regulation of its own expression in the presumptive caudal half of the somite.4 Publications

Inductioni

Down-regulated in NOTCH1, DLL1, RBPJ and FOXC1/FOXC2 mutant mices. Expression in the anterior presomitic mesoderm is periodic, dissipating once the segmental border is established. Negatively regulated by RIPPLY2. Positively regulated by TBX6 and Notch signaling.5 Publications

Gene expression databases

CleanExiMM_MESP2.

Structurei

3D structure databases

ProteinModelPortaliO08574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 13355bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni326 – 3305May contain a degradation domain

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi145 – 1506Poly-Arg

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG096371.
InParanoidiO08574.
KOiK09076.
PhylomeDBiO08574.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08574-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSSPPQSL QGLVPLGLLP GLGLGSAIGL HVSGLVLRFV RFLPFYATRR
60 70 80 90 100
PSQPAGPARS TRTTQATAPR RTRPAPAGGQ RQSASEREKL RMRTLARALQ
110 120 130 140 150
ELRRFLPPSV APAGQSLTKI ETLRLAIRYI GHLSALLGLS EDSLRRRRRR
160 170 180 190 200
SADAAFSHRC PQCPDGGSPS QAQMLGPSLG SAMSSGVSWG CPPACPGPLI
210 220 230 240 250
SPENLGNRIS NVDPRVTPPY CPQIQSPLHQ SLERAADSSP WAPPQACPGM
260 270 280 290 300
QMSPEPRNKT GHWTQSTEPA ELTKVYQSLS VSPEPRLSLG SPLLLPRPSC
310 320 330 340 350
QRLQPQPQPQ PQWGCWGHDA EVLSTSEDQG SSPALQLPVA SPTPSSGLQL
360 370
SGCPELWQED LEGPPLNIFY
Length:370
Mass (Da):39,789
Last modified:July 1, 1997 - v1
Checksum:i31CAB72E2398150D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071P → G in BAE27401 (PubMed:16141072).Curated
Sequence conflicti215 – 2151R → W in BAE27401 (PubMed:16141072).Curated
Sequence conflicti286 – 2861R → C in BAE27401 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71125 mRNA. Translation: AAB51199.1.
AK146740 mRNA. Translation: BAE27401.1.
RefSeqiNP_032615.2. NM_008589.2.
UniGeneiMm.31472.

Genome annotation databases

GeneIDi17293.
KEGGimmu:17293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71125 mRNA. Translation: AAB51199.1.
AK146740 mRNA. Translation: BAE27401.1.
RefSeqiNP_032615.2. NM_008589.2.
UniGeneiMm.31472.

3D structure databases

ProteinModelPortaliO08574.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO08574.
PhosphoSiteiO08574.

Proteomic databases

PRIDEiO08574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17293.
KEGGimmu:17293.

Organism-specific databases

CTDi145873.
MGIiMGI:1096325. Mesp2.

Phylogenomic databases

HOVERGENiHBG096371.
InParanoidiO08574.
KOiK09076.
PhylomeDBiO08574.

Miscellaneous databases

PROiO08574.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MESP2.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMESP2_MOUSE
AccessioniPrimary (citable) accession number: O08574
Secondary accession number(s): Q3UIV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.