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Protein

Phosphatidylinositol 4-kinase beta

Gene

Pi4kb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.1 Publication

Enzyme regulationi

Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ (By similarity).By similarity

GO - Molecular functioni

  • 1-phosphatidylinositol 4-kinase activity Source: RGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • phosphatidylinositol-mediated signaling Source: InterPro
  • signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660514. Synthesis of PIPs at the Golgi membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase beta (EC:2.7.1.67)
Short name:
PI4K-beta
Short name:
PI4Kbeta
Short name:
PtdIns 4-kinase beta
Gene namesi
Name:Pi4kb
Synonyms:Pik4cb
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621214. Pi4kb.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • Golgi apparatus Source: UniProtKB-SubCell
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • rough endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Phosphatidylinositol 4-kinase betaPRO_0000088831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei258 – 2581PhosphoserineBy similarity
Modified residuei263 – 2631PhosphothreonineBy similarity
Modified residuei266 – 2661PhosphoserineBy similarity
Modified residuei275 – 2751PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineBy similarity
Modified residuei284 – 2841PhosphoserineBy similarity
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei428 – 4281PhosphoserineCombined sources
Modified residuei438 – 4381PhosphothreonineBy similarity
Modified residuei511 – 5111PhosphoserineCombined sources
Modified residuei519 – 5191PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO08561.

Expressioni

Tissue specificityi

Strongly expressed in brain, kidney, lung, small intestine, uterus and adrenal gland. Weaker expression in liver, heart, skeletal muscle, thymus and testis. Not detected in spleen.1 Publication

Gene expression databases

ExpressionAtlasiO08561. baseline and differential.
GenevisibleiO08561. RN.

Interactioni

Subunit structurei

Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner. Interacts in a calcium-independent manner with NCS1/FREQ. This binding competes with CALN1/CABP8 and CALN2/CABP7 binding. Interacts with ARF1 and ARF3 (By similarity).By similarity

Protein-protein interaction databases

IntActiO08561. 4 interactions.
STRINGi10116.ENSRNOP00000039342.

Structurei

3D structure databases

ProteinModelPortaliO08561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 242182PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini558 – 765208PI3K/PI4KPROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0903. Eukaryota.
ENOG410XPH3. LUCA.
GeneTreeiENSGT00550000074892.
HOGENOMiHOG000008289.
HOVERGENiHBG053597.
InParanoidiO08561.
KOiK19801.
OMAiTFKRDPE.
OrthoDBiEOG773XFQ.
PhylomeDBiO08561.
TreeFamiTF102042.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTiSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAISS KGSPLELVNG DGVDNEIRCL DDPPTEIREE
110 120 130 140 150
EDEMEPGVVS GTAKGTRRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTNVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810
TTKLYDGFQY LTNGIM
Length:816
Mass (Da):91,655
Last modified:July 1, 1997 - v1
Checksum:iD7460076CE0AC362
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84667 mRNA. Translation: BAA18969.1.
RefSeqiNP_112345.1. NM_031083.1.
UniGeneiRn.14991.

Genome annotation databases

EnsembliENSRNOT00000040501; ENSRNOP00000039342; ENSRNOG00000021024.
GeneIDi81747.
KEGGirno:81747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84667 mRNA. Translation: BAA18969.1.
RefSeqiNP_112345.1. NM_031083.1.
UniGeneiRn.14991.

3D structure databases

ProteinModelPortaliO08561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08561. 4 interactions.
STRINGi10116.ENSRNOP00000039342.

Proteomic databases

PaxDbiO08561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040501; ENSRNOP00000039342; ENSRNOG00000021024.
GeneIDi81747.
KEGGirno:81747.

Organism-specific databases

CTDi5298.
RGDi621214. Pi4kb.

Phylogenomic databases

eggNOGiKOG0903. Eukaryota.
ENOG410XPH3. LUCA.
GeneTreeiENSGT00550000074892.
HOGENOMiHOG000008289.
HOVERGENiHBG053597.
InParanoidiO08561.
KOiK19801.
OMAiTFKRDPE.
OrthoDBiEOG773XFQ.
PhylomeDBiO08561.
TreeFamiTF102042.

Enzyme and pathway databases

ReactomeiR-RNO-1660514. Synthesis of PIPs at the Golgi membrane.

Miscellaneous databases

NextBioi615486.
PROiO08561.

Gene expression databases

ExpressionAtlasiO08561. baseline and differential.
GenevisibleiO08561. RN.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTiSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a 92 kDa soluble phosphatidylinositol 4-kinase."
    Nakagawa T., Goto K., Kondo H.
    Biochem. J. 320:643-649(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Wistar Imamichi1 Publication.
    Tissue: Brain1 Publication.
  2. "Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells."
    Zhao X., Varnai P., Tuymetova G., Balla A., Toth Z.E., Oker-Blom C., Roder J., Jeromin A., Balla T.
    J. Biol. Chem. 276:40183-40189(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCS1, SUBCELLULAR LOCATION.
  3. "Calneurons provide a calcium threshold for trans-Golgi network to plasma membrane trafficking."
    Mikhaylova M., Reddy P.P., Munsch T., Landgraf P., Suman S.K., Smalla K.-H., Gundelfinger E.D., Sharma Y., Kreutz M.R.
    Proc. Natl. Acad. Sci. U.S.A. 106:9093-9098(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CALN1/CABP8 AND CALN2/CABP7.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428 AND SER-511, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPI4KB_RAT
AccessioniPrimary (citable) accession number: O08561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 1, 1997
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.