Phosphatidylinositol 4-kinase beta - Rattus norvegicus (Rat)

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O08561 (PI4KB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase beta
Short name=PI4K-beta
Short name=PI4Kbeta
Short name=PtdIns 4-kinase beta
EC=2.7.1.67

Gene names

Name:	Pi4kb
Synonyms:	Pik4cb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. Ref.1
Enzyme regulation	Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ By similarity. Ref.1
Subunit structure	Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner. Interacts in a calcium-independent manner with NCS1/FREQ. This binding competes with CALN1/CABP8 and CALN2/CABP7 binding. Interacts with ARF1 and ARF3 By similarity. Ref.2 Ref.3
Subcellular location	Golgi apparatus. Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex By similarity. Ref.1 Ref.2 Ref.3
Tissue specificity	Strongly expressed in brain, kidney, lung, small intestine, uterus and adrenal gland. Weaker expression in liver, heart, skeletal muscle, thymus and testis. Not detected in spleen. Ref.1
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell rough endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from direct assay Ref.1. Source: RGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 816

816

Phosphatidylinositol 4-kinase beta

PRO_0000088831

Regions

Domain

61 – 242

182

PIK helical

Domain

558 – 765

208

PI3K/PI4K

Amino acid modifications

Modified residue

258

Phosphoserine By similarity UniProtKB Q9UBF8

Modified residue

263

Phosphothreonine By similarity UniProtKB Q9UBF8

Modified residue

266

Phosphoserine By similarity UniProtKB Q9UBF8

Modified residue

277

Phosphoserine By similarity UniProtKB Q9UBF8

Modified residue

280

Phosphothreonine By similarity

Modified residue

294

Phosphoserine By similarity UniProtKB Q9UBF8

Modified residue

428

Phosphoserine By similarity

Modified residue

438

Phosphothreonine By similarity UniProtKB Q9UBF8

Modified residue

511

Phosphoserine By similarity UniProtKB Q9UBF8

Modified residue

517

Phosphothreonine By similarity

Modified residue

519

Phosphothreonine By similarity UniProtKB Q9UBF8

Sequences

Sequence

Length

Mass (Da)

Tools

O08561 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: D7460076CE0AC362
Show »

FASTA

816

91,655

        10         20         30         40         50         60 
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 

        70         80         90        100        110        120 
LLHGGVAISS KGSPLELVNG DGVDNEIRCL DDPPTEIREE EDEMEPGVVS GTAKGTRRRR 

       130        140        150        160        170        180 
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 

       190        200        210        220        230        240 
NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 

       250        260        270        280        290        300 
LSDELKPAHR KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 

       310        320        330        340        350        360 
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 

       370        380        390        400        410        420 
HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTNVPARIPE 

       430        440        450        460        470        480 
NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 

       490        500        510        520        530        540 
NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 

       550        560        570        580        590        600 
VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 

       610        620        630        640        650        660 
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 

       670        680        690        700        710        720 
CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 

       730        740        750        760        770        780 
LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 

       790        800        810 
TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM

« Hide

References

[1]	"Cloning and characterization of a 92 kDa soluble phosphatidylinositol 4-kinase." Nakagawa T., Goto K., Kondo H. Biochem. J. 320:643-649(1996) [PubMed: 8973579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Wistar Imamichi. Tissue: Brain.
[2]	"Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells." Zhao X., Varnai P., Tuymetova G., Balla A., Toth Z.E., Oker-Blom C., Roder J., Jeromin A., Balla T. J. Biol. Chem. 276:40183-40189(2001) [PubMed: 11526106] [Abstract] Cited for: INTERACTION WITH NCS1, SUBCELLULAR LOCATION.
[3]	"Calneurons provide a calcium threshold for trans-Golgi network to plasma membrane trafficking." Mikhaylova M., Reddy P.P., Munsch T., Landgraf P., Suman S.K., Smalla K.-H., Gundelfinger E.D., Sharma Y., Kreutz M.R. Proc. Natl. Acad. Sci. U.S.A. 106:9093-9098(2009) [PubMed: 19458041] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CALN1/CABP8 AND CALN2/CABP7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84667 mRNA. Translation: BAA18969.1.
IPI	IPI00193760.
RefSeq	NP_112345.1. NM_031083.1.
UniGene	Rn.14991.
3D structure databases
ProteinModelPortal	O08561.
ModBase	Search...
Protein-protein interaction databases
STRING	O08561.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000040501; ENSRNOP00000039342; ENSRNOG00000021024.
GeneID	81747.
KEGG	rno:81747.
UCSC	NM_031083. rat.
Organism-specific databases
CTD	5298.
RGD	621214. Pi4kb.
Phylogenomic databases
eggNOG	roNOG09115.
GeneTree	ENSGT00550000074892.
HOVERGEN	HBG053597.
InParanoid	O08561.
OMA	PTAFRRD.
OrthoDB	EOG49078C.
PhylomeDB	O08561.
Gene expression databases
ArrayExpress	O08561.
Genevestigator	O08561.
GermOnline	ENSRNOG00000021024. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
KO	K00888.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615486.

Entry information

Entry name

PI4KB_RAT

Accession

Primary (citable) accession number: O08561

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents