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O08560

- DGKZ_RAT

UniProt

O08560 - DGKZ_RAT

Protein

Diacylglycerol kinase zeta

Gene

Dgkz

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: RGD
    3. enzyme inhibitor activity Source: Ensembl
    4. lipid kinase activity Source: Ensembl
    5. metal ion binding Source: UniProtKB-KW
    6. NAD+ kinase activity Source: InterPro
    7. protein binding Source: IntAct

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. mitotic G1 DNA damage checkpoint Source: Ensembl
    3. negative regulation of mitotic cell cycle Source: Ensembl
    4. negative regulation of Ras protein signal transduction Source: Ensembl
    5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.1.107. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase zeta (EC:2.7.1.107)
    Short name:
    DAG kinase zeta
    Alternative name(s):
    104 kDa diacylglycerol kinase
    DGK-IV
    Diglyceride kinase zeta
    Short name:
    DGK-zeta
    Gene namesi
    Name:Dgkz
    Synonyms:Dagk6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi70929. Dgkz.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus 1 Publication. Cell membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. lamellipodium Source: Ensembl
    3. nucleus Source: RGD
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Diacylglycerol kinase zetaPRO_0000218470Add
    BLAST

    Proteomic databases

    PaxDbiO08560.
    PRIDEiO08560.

    PTM databases

    PhosphoSiteiO08560.

    Expressioni

    Gene expression databases

    GenevestigatoriO08560.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dlg1Q626964EBI-8570505,EBI-389325
    Dlg2Q636224EBI-8570505,EBI-396947
    Dlg3Q629364EBI-8570505,EBI-349596
    Dlg4P310166EBI-8570505,EBI-375655

    Protein-protein interaction databases

    BioGridi249679. 3 interactions.
    IntActiO08560. 6 interactions.
    STRINGi10116.ENSRNOP00000060930.

    Structurei

    3D structure databases

    ProteinModelPortaliO08560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini292 – 426135DAGKcPROSITE-ProRule annotationAdd
    BLAST
    Repeati823 – 85331ANK 1Add
    BLAST
    Repeati858 – 88730ANK 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni279 – 417139Mediates interaction with RASGRP1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi362 – 3709Nuclear export signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi67 – 726Poly-Pro
    Compositional biasi260 – 2634Poly-Lys
    Compositional biasi372 – 3754Poly-Pro

    Sequence similaritiesi

    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00740000115137.
    HOGENOMiHOG000231472.
    HOVERGENiHBG067303.
    InParanoidiO08560.
    KOiK00901.
    OMAiQQEPDGA.
    OrthoDBiEOG76HQ0Q.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O08560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPRDPSPEA RSSDSESASA SSSGSERDAD PEPDKAPRRL TKRRFPGLRL    50
    FGHRKAITKS GLQHLAPPPP TPGAPCGESE RQIRSTVDWS ESAAYGEHIW 100
    FETNVSGDFC YVGEQYCVAK MLPKSAPRRK CAACKIVVHT PCIGQLEKIN 150
    FRCKPSFRES GSRNVREPTF VRHHWVHRRR QDGKCRHCGK GFQQKFTFHS 200
    KEIVAISCSW CKQAYHSKVS CFMLQQIEEP CSLGVHAAVV IPPTWILRAR 250
    RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF 300
    VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL 350
    ACGGDGTVGW ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE 400
    PVSKILSHVE EGNVVQLDRW DLRAEPNPEA GPEERDDGAT DRLPLDVFNN 450
    YFSLGFDAHV TLEFHESREA NPEKFNSRFR NKMFYAGTAF SDFLMGSSKD 500
    LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP WGHPGEHHDF 550
    EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV 600
    DGEPCKLAAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS 650
    RVSMHDYEAL HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQQEPD 700
    GAGAKSPMCH PLSSKWCFLD ATTASRFYRI DRAQEHLNYV TEIAQDEIYI 750
    LDPELLGASA RPDLPTPTSP LPASPCSPTP GSLQGDAALP QGEELIEAAK 800
    RNDFCKLQEL HRAGGDLMHR DHQSRTLLHH AVSTGSKEVV RYLLDHAPPE 850
    ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDQ QGDTPRQRAE 900
    KAQDTELAAY LENRQHYQMI QREDQETAV 929
    Length:929
    Mass (Da):104,009
    Last modified:July 1, 1997 - v1
    Checksum:i6279872004F5F38D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78588 mRNA. Translation: BAA18942.1.
    PIRiJC6124.
    RefSeqiNP_112405.1. NM_031143.1.
    UniGeneiRn.11208.

    Genome annotation databases

    EnsembliENSRNOT00000024280; ENSRNOP00000024280; ENSRNOG00000017737.
    GeneIDi81821.
    KEGGirno:81821.
    UCSCiRGD:70929. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78588 mRNA. Translation: BAA18942.1 .
    PIRi JC6124.
    RefSeqi NP_112405.1. NM_031143.1.
    UniGenei Rn.11208.

    3D structure databases

    ProteinModelPortali O08560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249679. 3 interactions.
    IntActi O08560. 6 interactions.
    STRINGi 10116.ENSRNOP00000060930.

    PTM databases

    PhosphoSitei O08560.

    Proteomic databases

    PaxDbi O08560.
    PRIDEi O08560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024280 ; ENSRNOP00000024280 ; ENSRNOG00000017737 .
    GeneIDi 81821.
    KEGGi rno:81821.
    UCSCi RGD:70929. rat.

    Organism-specific databases

    CTDi 8525.
    RGDi 70929. Dgkz.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00740000115137.
    HOGENOMi HOG000231472.
    HOVERGENi HBG067303.
    InParanoidi O08560.
    KOi K00901.
    OMAi QQEPDGA.
    OrthoDBi EOG76HQ0Q.

    Enzyme and pathway databases

    BRENDAi 2.7.1.107. 5301.

    Miscellaneous databases

    NextBioi 615743.
    PROi O08560.

    Gene expression databases

    Genevestigatori O08560.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus."
      Goto K., Kondo H.
      Proc. Natl. Acad. Sci. U.S.A. 93:11196-11201(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: Wistar.

    Entry informationi

    Entry nameiDGKZ_RAT
    AccessioniPrimary (citable) accession number: O08560
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3