Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diacylglycerol kinase zeta

Gene

Dgkz

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107. 5301.
ReactomeiREACT_351116. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase zeta (EC:2.7.1.107)
Short name:
DAG kinase zeta
Alternative name(s):
104 kDa diacylglycerol kinase
DGK-IV
Diglyceride kinase zeta
Short name:
DGK-zeta
Gene namesi
Name:Dgkz
Synonyms:Dagk6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi70929. Dgkz.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • lamellipodium Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Diacylglycerol kinase zetaPRO_0000218470Add
BLAST

Proteomic databases

PaxDbiO08560.
PRIDEiO08560.

PTM databases

PhosphoSiteiO08560.

Expressioni

Gene expression databases

GenevisibleiO08560. RN.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg1Q626964EBI-8570505,EBI-389325
Dlg2Q636224EBI-8570505,EBI-396947
Dlg3Q629364EBI-8570505,EBI-349596
Dlg4P310166EBI-8570505,EBI-375655

Protein-protein interaction databases

BioGridi249679. 3 interactions.
IntActiO08560. 6 interactions.
STRINGi10116.ENSRNOP00000024280.

Structurei

3D structure databases

ProteinModelPortaliO08560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini292 – 426135DAGKcPROSITE-ProRule annotationAdd
BLAST
Repeati823 – 85331ANK 1Add
BLAST
Repeati858 – 88730ANK 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni279 – 417139Mediates interaction with RASGRP1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi362 – 3709Nuclear export signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi67 – 726Poly-Pro
Compositional biasi260 – 2634Poly-Lys
Compositional biasi372 – 3754Poly-Pro

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 15356Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri173 – 23159Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiO08560.
KOiK00901.
OMAiQQEPDGA.
OrthoDBiEOG76HQ0Q.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O08560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRDPSPEA RSSDSESASA SSSGSERDAD PEPDKAPRRL TKRRFPGLRL
60 70 80 90 100
FGHRKAITKS GLQHLAPPPP TPGAPCGESE RQIRSTVDWS ESAAYGEHIW
110 120 130 140 150
FETNVSGDFC YVGEQYCVAK MLPKSAPRRK CAACKIVVHT PCIGQLEKIN
160 170 180 190 200
FRCKPSFRES GSRNVREPTF VRHHWVHRRR QDGKCRHCGK GFQQKFTFHS
210 220 230 240 250
KEIVAISCSW CKQAYHSKVS CFMLQQIEEP CSLGVHAAVV IPPTWILRAR
260 270 280 290 300
RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF
310 320 330 340 350
VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL
360 370 380 390 400
ACGGDGTVGW ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE
410 420 430 440 450
PVSKILSHVE EGNVVQLDRW DLRAEPNPEA GPEERDDGAT DRLPLDVFNN
460 470 480 490 500
YFSLGFDAHV TLEFHESREA NPEKFNSRFR NKMFYAGTAF SDFLMGSSKD
510 520 530 540 550
LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP WGHPGEHHDF
560 570 580 590 600
EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV
610 620 630 640 650
DGEPCKLAAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS
660 670 680 690 700
RVSMHDYEAL HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQQEPD
710 720 730 740 750
GAGAKSPMCH PLSSKWCFLD ATTASRFYRI DRAQEHLNYV TEIAQDEIYI
760 770 780 790 800
LDPELLGASA RPDLPTPTSP LPASPCSPTP GSLQGDAALP QGEELIEAAK
810 820 830 840 850
RNDFCKLQEL HRAGGDLMHR DHQSRTLLHH AVSTGSKEVV RYLLDHAPPE
860 870 880 890 900
ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDQ QGDTPRQRAE
910 920
KAQDTELAAY LENRQHYQMI QREDQETAV
Length:929
Mass (Da):104,009
Last modified:July 1, 1997 - v1
Checksum:i6279872004F5F38D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78588 mRNA. Translation: BAA18942.1.
PIRiJC6124.
RefSeqiNP_112405.1. NM_031143.1.
UniGeneiRn.11208.

Genome annotation databases

EnsembliENSRNOT00000024280; ENSRNOP00000024280; ENSRNOG00000017737.
GeneIDi81821.
KEGGirno:81821.
UCSCiRGD:70929. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78588 mRNA. Translation: BAA18942.1.
PIRiJC6124.
RefSeqiNP_112405.1. NM_031143.1.
UniGeneiRn.11208.

3D structure databases

ProteinModelPortaliO08560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249679. 3 interactions.
IntActiO08560. 6 interactions.
STRINGi10116.ENSRNOP00000024280.

PTM databases

PhosphoSiteiO08560.

Proteomic databases

PaxDbiO08560.
PRIDEiO08560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024280; ENSRNOP00000024280; ENSRNOG00000017737.
GeneIDi81821.
KEGGirno:81821.
UCSCiRGD:70929. rat.

Organism-specific databases

CTDi8525.
RGDi70929. Dgkz.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiO08560.
KOiK00901.
OMAiQQEPDGA.
OrthoDBiEOG76HQ0Q.

Enzyme and pathway databases

BRENDAi2.7.1.107. 5301.
ReactomeiREACT_351116. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi615743.
PROiO08560.

Gene expression databases

GenevisibleiO08560. RN.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus."
    Goto K., Kondo H.
    Proc. Natl. Acad. Sci. U.S.A. 93:11196-11201(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: Wistar.

Entry informationi

Entry nameiDGKZ_RAT
AccessioniPrimary (citable) accession number: O08560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.