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O08560 (DGKZ_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Diacylglycerol kinase zeta
Short name=DAG kinase zeta
EC=2.7.1.107
Alternative name(s):
104 kDa diacylglycerol kinase
DGK-IV
Diglyceride kinase zeta
Short name=DGK-zeta
Gene names
Name:Dgkz
Synonyms:Dagk6
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity By similarity.

Catalytic activity

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Subunit structure

Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Forms a signaling complex with RASGRP1 and HRAS By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus. Cell membrane By similarity Ref.1.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 2 ANK repeats.

Contains 1 DAGKc domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Diacylglycerol kinase zeta
PRO_0000218470

Regions

Domain292 – 426135DAGKc
Repeat823 – 85331ANK 1
Repeat858 – 88730ANK 2
Zinc finger98 – 15356Phorbol-ester/DAG-type 1
Zinc finger173 – 23159Phorbol-ester/DAG-type 2
Region279 – 417139Mediates interaction with RASGRP1 By similarity
Motif362 – 3709Nuclear export signal By similarity
Compositional bias67 – 726Poly-Pro
Compositional bias260 – 2634Poly-Lys
Compositional bias372 – 3754Poly-Pro

Amino acid modifications

Modified residue4981Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
O08560 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6279872004F5F38D

FASTA929104,009
        10         20         30         40         50         60 
MEPRDPSPEA RSSDSESASA SSSGSERDAD PEPDKAPRRL TKRRFPGLRL FGHRKAITKS 

        70         80         90        100        110        120 
GLQHLAPPPP TPGAPCGESE RQIRSTVDWS ESAAYGEHIW FETNVSGDFC YVGEQYCVAK 

       130        140        150        160        170        180 
MLPKSAPRRK CAACKIVVHT PCIGQLEKIN FRCKPSFRES GSRNVREPTF VRHHWVHRRR 

       190        200        210        220        230        240 
QDGKCRHCGK GFQQKFTFHS KEIVAISCSW CKQAYHSKVS CFMLQQIEEP CSLGVHAAVV 

       250        260        270        280        290        300 
IPPTWILRAR RPQNTLKASK KKKRASFKRR SSKKGPEEGR WRPFIIRPTP SPLMKPLLVF 

       310        320        330        340        350        360 
VNPKSGGNQG AKIIQSFLWY LNPRQVFDLS QGGPREALEM YRKVHNLRIL ACGGDGTVGW 

       370        380        390        400        410        420 
ILSTLDQLRL KPPPPVAILP LGTGNDLART LNWGGGYTDE PVSKILSHVE EGNVVQLDRW 

       430        440        450        460        470        480 
DLRAEPNPEA GPEERDDGAT DRLPLDVFNN YFSLGFDAHV TLEFHESREA NPEKFNSRFR 

       490        500        510        520        530        540 
NKMFYAGTAF SDFLMGSSKD LAKHIRVVCD GMDLTPKIQD LKPQCIVFLN IPRYCAGTMP 

       550        560        570        580        590        600 
WGHPGEHHDF EPQRHDDGYL EVIGFTMTSL AALQVGGHGE RLTQCREVLL TTAKAIPVQV 

       610        620        630        640        650        660 
DGEPCKLAAS RIRIALRNQA TMVQKAKRRS TAPLHSDQQP VPEQLRIQVS RVSMHDYEAL 

       670        680        690        700        710        720 
HYDKEQLKEA SVPLGTVVVP GDSDLELCRA HIERLQQEPD GAGAKSPMCH PLSSKWCFLD 

       730        740        750        760        770        780 
ATTASRFYRI DRAQEHLNYV TEIAQDEIYI LDPELLGASA RPDLPTPTSP LPASPCSPTP 

       790        800        810        820        830        840 
GSLQGDAALP QGEELIEAAK RNDFCKLQEL HRAGGDLMHR DHQSRTLLHH AVSTGSKEVV 

       850        860        870        880        890        900 
RYLLDHAPPE ILDAVEENGE TCLHQAAALG QRTICHYIVE AGASLMKTDQ QGDTPRQRAE 

       910        920 
KAQDTELAAY LENRQHYQMI QREDQETAV

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References

[1]"A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus."
Goto K., Kondo H.
Proc. Natl. Acad. Sci. U.S.A. 93:11196-11201(1996) [PubMed: 8855332] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: Wistar.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D78588 mRNA. Translation: BAA18942.1.
IPIIPI00193758.
PIRJC6124.
RefSeqNP_112405.1. NM_031143.1.
UniGeneRn.11208.

3D structure databases

ProteinModelPortalO08560.
ModBaseSearch...

Protein-protein interaction databases

STRINGO08560.

PTM databases

PhosphoSiteO08560.

Proteomic databases

PRIDEO08560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024280; ENSRNOP00000024280; ENSRNOG00000017737.
GeneID81821.
KEGGrno:81821.
UCSCNM_031143. rat.

Organism-specific databases

CTD8525.
RGD70929. Dgkz.

Phylogenomic databases

eggNOGroNOG04094.
GeneTreeENSGT00600000084185.
HOVERGENHBG067303.
InParanoidO08560.
OMAGDLMHRD.
PhylomeDBO08560.

Enzyme and pathway databases

BRENDA2.7.1.107. 5301.

Gene expression databases

ArrayExpressO08560.
GenevestigatorO08560.
GermOnlineENSRNOG00000017737. Rattus norvegicus.

Family and domain databases

InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
Gene3DG3DSA:1.25.40.20. ANK. 2 hits.
KOK00901.
PfamPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. False negative.
PS50081. ZF_DAG_PE_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio615743.

Entry information

Entry nameDGKZ_RAT
AccessionPrimary (citable) accession number: O08560
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 16, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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