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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30Substrate; via carbonyl oxygenBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei78SubstrateBy similarity1
Binding sitei79SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei145SubstrateBy similarity1
Active sitei173Proton donorBy similarity1
Binding sitei268Substrate; via carbonyl oxygenBy similarity1
Active sitei274NucleophileBy similarity1
Metal bindingi274ZincBy similarity1

GO - Molecular functioni

  • amino acid binding Source: RGD
  • dimethylargininase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.3.18 5301
ReactomeiR-RNO-203615 eNOS activation
SABIO-RKiO08557

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
Synonyms:Ddah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi70968 Ddah1

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4196

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001711202 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 1Add BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei33PhosphoserineCombined sources1
Modified residuei222S-nitrosocysteineBy similarity1
Modified residuei274S-nitrosocysteineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiO08557
PRIDEiO08557

PTM databases

iPTMnetiO08557
PhosphoSitePlusiO08557
SwissPalmiO08557

Expressioni

Tissue specificityi

Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication

Gene expression databases

BgeeiENSRNOG00000014613
GenevisibleiO08557 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044971

Chemistry databases

BindingDBiO08557

Structurei

3D structure databases

ProteinModelPortaliO08557
SMRiO08557
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410IFS6 Eukaryota
COG1834 LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiO08557
KOiK01482
OMAiCNGIALI
OrthoDBiEOG091G0M5S
PhylomeDBiO08557

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033197 DDAH1
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF17 PTHR12737:SF17, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS
Length:285
Mass (Da):31,426
Last modified:January 23, 2007 - v3
Checksum:i9FE8DF56E37A7E4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86041 mRNA Translation: BAA18993.1
RefSeqiNP_071633.1, NM_022297.2
UniGeneiRn.7398

Genome annotation databases

EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613
GeneIDi64157
KEGGirno:64157

Similar proteinsi

Entry informationi

Entry nameiDDAH1_RAT
AccessioniPrimary (citable) accession number: O08557
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health