Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O08557

- DDAH1_RAT

UniProt

O08557 - DDAH1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301Substrate; via carbonyl oxygenBy similarity
Binding sitei73 – 731SubstrateBy similarity
Binding sitei78 – 781SubstrateBy similarity
Binding sitei79 – 791SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei145 – 1451SubstrateBy similarity
Active sitei173 – 1731Proton donorBy similarity
Binding sitei268 – 2681Substrate; via carbonyl oxygenBy similarity
Active sitei274 – 2741NucleophileBy similarity
Metal bindingi274 – 2741ZincBy similarity

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. dimethylargininase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine metabolic process Source: RGD
  2. citrulline metabolic process Source: UniProtKB
  3. positive regulation of angiogenesis Source: RGD
  4. positive regulation of nitric oxide biosynthetic process Source: RGD
  5. regulation of systemic arterial blood pressure Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
Synonyms:Ddah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi70968. Ddah1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. mitochondrion Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 285284N(G),N(G)-dimethylarginine dimethylaminohydrolase 1PRO_0000171120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei222 – 2221S-nitrosocysteineBy similarity
Modified residuei274 – 2741S-nitrosocysteineBy similarity

Keywords - PTMi

Acetylation, S-nitrosylation

Proteomic databases

PaxDbiO08557.
PRIDEiO08557.

PTM databases

PhosphoSiteiO08557.

Expressioni

Tissue specificityi

Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication

Gene expression databases

GenevestigatoriO08557.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-4567619.
STRINGi10116.ENSRNOP00000044971.

Structurei

3D structure databases

ProteinModelPortaliO08557.
SMRiO08557. Positions 8-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiCOG1834.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiO08557.
KOiK01482.
OMAiKTMQQMS.
OrthoDBiEOG73V6KV.
PhylomeDBiO08557.

Family and domain databases

InterProiIPR003198. Amidino_trans.
[Graphical view]
PfamiPF02274. Amidinotransf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08557-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS
Length:285
Mass (Da):31,426
Last modified:January 23, 2007 - v3
Checksum:i9FE8DF56E37A7E4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86041 mRNA. Translation: BAA18993.1.
RefSeqiNP_071633.1. NM_022297.2.
UniGeneiRn.7398.

Genome annotation databases

EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613.
GeneIDi64157.
KEGGirno:64157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86041 mRNA. Translation: BAA18993.1 .
RefSeqi NP_071633.1. NM_022297.2.
UniGenei Rn.7398.

3D structure databases

ProteinModelPortali O08557.
SMRi O08557. Positions 8-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4567619.
STRINGi 10116.ENSRNOP00000044971.

Chemistry

BindingDBi O08557.
ChEMBLi CHEMBL4196.

PTM databases

PhosphoSitei O08557.

Proteomic databases

PaxDbi O08557.
PRIDEi O08557.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000045016 ; ENSRNOP00000044971 ; ENSRNOG00000014613 .
GeneIDi 64157.
KEGGi rno:64157.

Organism-specific databases

CTDi 23576.
RGDi 70968. Ddah1.

Phylogenomic databases

eggNOGi COG1834.
GeneTreei ENSGT00390000009331.
HOGENOMi HOG000161035.
HOVERGENi HBG055937.
InParanoidi O08557.
KOi K01482.
OMAi KTMQQMS.
OrthoDBi EOG73V6KV.
PhylomeDBi O08557.

Miscellaneous databases

NextBioi 612794.
PROi O08557.

Gene expression databases

Genevestigatori O08557.

Family and domain databases

InterProi IPR003198. Amidino_trans.
[Graphical view ]
Pfami PF02274. Amidinotransf. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney."
    Kimoto M., Sasakawa T., Tsuji H., Miyatake S., Oka T., Nio N., Ogawa T.
    Biochim. Biophys. Acta 1337:6-10(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT ALA-2.
    Strain: Wistar.
    Tissue: Kidney.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 46-57; 112-136 AND 160-175, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  3. "Contribution of whole blood to the control of plasma asymmetrical dimethylarginine."
    Billecke S.S., Kitzmiller L.A., Northrup J.J., Whitesall S.E., Kimoto M., Hinz A.V., D'Alecy L.G.
    Am. J. Physiol. 291:H1788-H1796(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDDAH1_RAT
AccessioniPrimary (citable) accession number: O08557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3