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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30Substrate; via carbonyl oxygenBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei78SubstrateBy similarity1
Binding sitei79SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei145SubstrateBy similarity1
Active sitei173Proton donorBy similarity1
Binding sitei268Substrate; via carbonyl oxygenBy similarity1
Active sitei274NucleophileBy similarity1
Metal bindingi274ZincBy similarity1

GO - Molecular functioni

  • amino acid binding Source: RGD
  • dimethylargininase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • arginine catabolic process Source: InterPro
  • arginine metabolic process Source: RGD
  • citrulline metabolic process Source: UniProtKB
  • positive regulation of angiogenesis Source: RGD
  • positive regulation of nitric oxide biosynthetic process Source: RGD
  • regulation of systemic arterial blood pressure Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.3.18. 5301.
ReactomeiR-RNO-203615. eNOS activation.
SABIO-RKO08557.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
Synonyms:Ddah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi70968. Ddah1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4196.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001711202 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 1Add BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei33PhosphoserineCombined sources1
Modified residuei222S-nitrosocysteineBy similarity1
Modified residuei274S-nitrosocysteineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiO08557.
PRIDEiO08557.

PTM databases

iPTMnetiO08557.
PhosphoSitePlusiO08557.

Expressioni

Tissue specificityi

Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication

Gene expression databases

BgeeiENSRNOG00000014613.
GenevisibleiO08557. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-4567619.
STRINGi10116.ENSRNOP00000044971.

Chemistry databases

BindingDBiO08557.

Structurei

3D structure databases

ProteinModelPortaliO08557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410IFS6. Eukaryota.
COG1834. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiO08557.
KOiK01482.
OMAiCNGIALI.
OrthoDBiEOG091G0M5S.
PhylomeDBiO08557.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033197. DDAH1.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF17. PTHR12737:SF17. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS
Length:285
Mass (Da):31,426
Last modified:January 23, 2007 - v3
Checksum:i9FE8DF56E37A7E4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86041 mRNA. Translation: BAA18993.1.
RefSeqiNP_071633.1. NM_022297.2.
UniGeneiRn.7398.

Genome annotation databases

EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613.
GeneIDi64157.
KEGGirno:64157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86041 mRNA. Translation: BAA18993.1.
RefSeqiNP_071633.1. NM_022297.2.
UniGeneiRn.7398.

3D structure databases

ProteinModelPortaliO08557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4567619.
STRINGi10116.ENSRNOP00000044971.

Chemistry databases

BindingDBiO08557.
ChEMBLiCHEMBL4196.

PTM databases

iPTMnetiO08557.
PhosphoSitePlusiO08557.

Proteomic databases

PaxDbiO08557.
PRIDEiO08557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613.
GeneIDi64157.
KEGGirno:64157.

Organism-specific databases

CTDi23576.
RGDi70968. Ddah1.

Phylogenomic databases

eggNOGiENOG410IFS6. Eukaryota.
COG1834. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiO08557.
KOiK01482.
OMAiCNGIALI.
OrthoDBiEOG091G0M5S.
PhylomeDBiO08557.

Enzyme and pathway databases

BRENDAi3.5.3.18. 5301.
ReactomeiR-RNO-203615. eNOS activation.
SABIO-RKO08557.

Miscellaneous databases

PROiO08557.

Gene expression databases

BgeeiENSRNOG00000014613.
GenevisibleiO08557. RN.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033197. DDAH1.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF17. PTHR12737:SF17. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH1_RAT
AccessioniPrimary (citable) accession number: O08557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.