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O08557

- DDAH1_RAT

UniProt

O08557 - DDAH1_RAT

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Protein
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Gene
Ddah1, Ddah
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301Substrate; via carbonyl oxygen By similarity
Binding sitei73 – 731Substrate By similarity
Binding sitei78 – 781Substrate By similarity
Binding sitei79 – 791Substrate By similarity
Binding sitei98 – 981Substrate By similarity
Binding sitei145 – 1451Substrate By similarity
Active sitei173 – 1731Proton donor By similarity
Binding sitei268 – 2681Substrate; via carbonyl oxygen By similarity
Active sitei274 – 2741Nucleophile By similarity
Metal bindingi274 – 2741Zinc By similarity

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. dimethylargininase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine metabolic process Source: RGD
  2. citrulline metabolic process Source: UniProtKB
  3. positive regulation of angiogenesis Source: RGD
  4. positive regulation of nitric oxide biosynthetic process Source: RGD
  5. regulation of systemic arterial blood pressure Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
Synonyms:Ddah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi70968. Ddah1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 285284N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
PRO_0000171120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei222 – 2221S-nitrosocysteine By similarity
Modified residuei274 – 2741S-nitrosocysteine By similarity

Keywords - PTMi

Acetylation, S-nitrosylation

Proteomic databases

PaxDbiO08557.
PRIDEiO08557.

PTM databases

PhosphoSiteiO08557.

Expressioni

Tissue specificityi

Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication

Gene expression databases

GenevestigatoriO08557.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

MINTiMINT-4567619.
STRINGi10116.ENSRNOP00000044971.

Structurei

3D structure databases

ProteinModelPortaliO08557.
SMRiO08557. Positions 8-281.

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.

Phylogenomic databases

eggNOGiCOG1834.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
KOiK01482.
OMAiKTMQQMS.
OrthoDBiEOG73V6KV.
PhylomeDBiO08557.

Family and domain databases

InterProiIPR003198. Amidino_trans.
[Graphical view]
PfamiPF02274. Amidinotransf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08557-1 [UniParc]FASTAAdd to Basket

« Hide

MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY    50
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK 100
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG 150
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI 200
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE 250
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS 285
Length:285
Mass (Da):31,426
Last modified:January 23, 2007 - v3
Checksum:i9FE8DF56E37A7E4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86041 mRNA. Translation: BAA18993.1.
RefSeqiNP_071633.1. NM_022297.2.
UniGeneiRn.7398.

Genome annotation databases

EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613.
GeneIDi64157.
KEGGirno:64157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86041 mRNA. Translation: BAA18993.1 .
RefSeqi NP_071633.1. NM_022297.2.
UniGenei Rn.7398.

3D structure databases

ProteinModelPortali O08557.
SMRi O08557. Positions 8-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4567619.
STRINGi 10116.ENSRNOP00000044971.

Chemistry

BindingDBi O08557.
ChEMBLi CHEMBL4196.

PTM databases

PhosphoSitei O08557.

Proteomic databases

PaxDbi O08557.
PRIDEi O08557.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000045016 ; ENSRNOP00000044971 ; ENSRNOG00000014613 .
GeneIDi 64157.
KEGGi rno:64157.

Organism-specific databases

CTDi 23576.
RGDi 70968. Ddah1.

Phylogenomic databases

eggNOGi COG1834.
GeneTreei ENSGT00390000009331.
HOGENOMi HOG000161035.
HOVERGENi HBG055937.
KOi K01482.
OMAi KTMQQMS.
OrthoDBi EOG73V6KV.
PhylomeDBi O08557.

Miscellaneous databases

NextBioi 612794.
PROi O08557.

Gene expression databases

Genevestigatori O08557.

Family and domain databases

InterProi IPR003198. Amidino_trans.
[Graphical view ]
Pfami PF02274. Amidinotransf. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney."
    Kimoto M., Sasakawa T., Tsuji H., Miyatake S., Oka T., Nio N., Ogawa T.
    Biochim. Biophys. Acta 1337:6-10(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT ALA-2.
    Strain: Wistar.
    Tissue: Kidney.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 46-57; 112-136 AND 160-175, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  3. "Contribution of whole blood to the control of plasma asymmetrical dimethylarginine."
    Billecke S.S., Kitzmiller L.A., Northrup J.J., Whitesall S.E., Kimoto M., Hinz A.V., D'Alecy L.G.
    Am. J. Physiol. 291:H1788-H1796(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDDAH1_RAT
AccessioniPrimary (citable) accession number: O08557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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