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O08557

- DDAH1_RAT

UniProt

O08557 - DDAH1_RAT

Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

    Catalytic activityi

    N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

    Enzyme regulationi

    Inhibited by zinc ions.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301Substrate; via carbonyl oxygenBy similarity
    Binding sitei73 – 731SubstrateBy similarity
    Binding sitei78 – 781SubstrateBy similarity
    Binding sitei79 – 791SubstrateBy similarity
    Binding sitei98 – 981SubstrateBy similarity
    Binding sitei145 – 1451SubstrateBy similarity
    Active sitei173 – 1731Proton donorBy similarity
    Binding sitei268 – 2681Substrate; via carbonyl oxygenBy similarity
    Active sitei274 – 2741NucleophileBy similarity
    Metal bindingi274 – 2741ZincBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: RGD
    2. dimethylargininase activity Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginine metabolic process Source: RGD
    2. citrulline metabolic process Source: UniProtKB
    3. positive regulation of angiogenesis Source: RGD
    4. positive regulation of nitric oxide biosynthetic process Source: RGD
    5. regulation of systemic arterial blood pressure Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
    Short name:
    DDAH-1
    Short name:
    Dimethylarginine dimethylaminohydrolase 1
    Alternative name(s):
    DDAHI
    Dimethylargininase-1
    Gene namesi
    Name:Ddah1
    Synonyms:Ddah
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi70968. Ddah1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 285284N(G),N(G)-dimethylarginine dimethylaminohydrolase 1PRO_0000171120Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei222 – 2221S-nitrosocysteineBy similarity
    Modified residuei274 – 2741S-nitrosocysteineBy similarity

    Keywords - PTMi

    Acetylation, S-nitrosylation

    Proteomic databases

    PaxDbiO08557.
    PRIDEiO08557.

    PTM databases

    PhosphoSiteiO08557.

    Expressioni

    Tissue specificityi

    Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication

    Gene expression databases

    GenevestigatoriO08557.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    MINTiMINT-4567619.
    STRINGi10116.ENSRNOP00000044971.

    Structurei

    3D structure databases

    ProteinModelPortaliO08557.
    SMRiO08557. Positions 8-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DDAH family.Curated

    Phylogenomic databases

    eggNOGiCOG1834.
    GeneTreeiENSGT00390000009331.
    HOGENOMiHOG000161035.
    HOVERGENiHBG055937.
    KOiK01482.
    OMAiKTMQQMS.
    OrthoDBiEOG73V6KV.
    PhylomeDBiO08557.

    Family and domain databases

    InterProiIPR003198. Amidino_trans.
    [Graphical view]
    PfamiPF02274. Amidinotransf. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08557-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY    50
    VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK 100
    EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG 150
    AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI 200
    MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE 250
    KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS 285
    Length:285
    Mass (Da):31,426
    Last modified:January 23, 2007 - v3
    Checksum:i9FE8DF56E37A7E4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86041 mRNA. Translation: BAA18993.1.
    RefSeqiNP_071633.1. NM_022297.2.
    UniGeneiRn.7398.

    Genome annotation databases

    EnsembliENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613.
    GeneIDi64157.
    KEGGirno:64157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86041 mRNA. Translation: BAA18993.1 .
    RefSeqi NP_071633.1. NM_022297.2.
    UniGenei Rn.7398.

    3D structure databases

    ProteinModelPortali O08557.
    SMRi O08557. Positions 8-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4567619.
    STRINGi 10116.ENSRNOP00000044971.

    Chemistry

    BindingDBi O08557.
    ChEMBLi CHEMBL4196.

    PTM databases

    PhosphoSitei O08557.

    Proteomic databases

    PaxDbi O08557.
    PRIDEi O08557.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000045016 ; ENSRNOP00000044971 ; ENSRNOG00000014613 .
    GeneIDi 64157.
    KEGGi rno:64157.

    Organism-specific databases

    CTDi 23576.
    RGDi 70968. Ddah1.

    Phylogenomic databases

    eggNOGi COG1834.
    GeneTreei ENSGT00390000009331.
    HOGENOMi HOG000161035.
    HOVERGENi HBG055937.
    KOi K01482.
    OMAi KTMQQMS.
    OrthoDBi EOG73V6KV.
    PhylomeDBi O08557.

    Miscellaneous databases

    NextBioi 612794.
    PROi O08557.

    Gene expression databases

    Genevestigatori O08557.

    Family and domain databases

    InterProi IPR003198. Amidino_trans.
    [Graphical view ]
    Pfami PF02274. Amidinotransf. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of cDNA encoding NG,NG-dimethylarginine dimethylaminohydrolase from rat kidney."
      Kimoto M., Sasakawa T., Tsuji H., Miyatake S., Oka T., Nio N., Ogawa T.
      Biochim. Biophys. Acta 1337:6-10(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT ALA-2.
      Strain: Wistar.
      Tissue: Kidney.
    2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 46-57; 112-136 AND 160-175, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.
    3. "Contribution of whole blood to the control of plasma asymmetrical dimethylarginine."
      Billecke S.S., Kitzmiller L.A., Northrup J.J., Whitesall S.E., Kimoto M., Hinz A.V., D'Alecy L.G.
      Am. J. Physiol. 291:H1788-H1796(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiDDAH1_RAT
    AccessioniPrimary (citable) accession number: O08557
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3