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O08556 (CCR5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C chemokine receptor type 5

Short name=C-C CKR-5
Short name=CC-CKR-5
Short name=CCR-5
Alternative name(s):
MIP-1 alpha receptor
CD_antigen=CD195
Gene names
Name:Ccr5
Synonyms:Cmkbr5
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation By similarity.

Subunit structure

Interacts with PRAF2. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Sulfated on at least 2 of the N-terminal tyrosines. Sulfation is required for efficient binding of the chemokines, CCL3 and CCL4 By similarity.

O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen By similarity.

Palmitoylation in the C-terminal is important for cell surface expression By similarity.

Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from expression pattern PubMed 16369869. Source: RGD

blood vessel remodeling

Inferred from electronic annotation. Source: InterPro

chemokine-mediated signaling pathway

Traceable author statement PubMed 12403770. Source: GOC

chemotaxis

Inferred from electronic annotation. Source: InterPro

defense response to bacterium

Inferred from expression pattern Ref.1. Source: RGD

fat cell differentiation

Inferred from expression pattern PubMed 17286602. Source: RGD

forebrain development

Inferred from expression pattern PubMed 16516309. Source: RGD

inflammatory response

Inferred from expression pattern PubMed 12144807. Source: RGD

inflammatory response to antigenic stimulus

Inferred from expression pattern PubMed 14674010. Source: RGD

leukocyte migration

Traceable author statement PubMed 12403770. Source: RGD

negative regulation of axon extension

Inferred from mutant phenotype PubMed 16537566. Source: RGD

negative regulation of cell migration

Inferred from mutant phenotype PubMed 20940264. Source: RGD

negative regulation of neural precursor cell proliferation

Inferred from mutant phenotype PubMed 20940264. Source: RGD

neuron death

Inferred from mutant phenotype PubMed 20940264. Source: RGD

positive regulation of apoptotic process by virus

Inferred from mutant phenotype PubMed 19034668. Source: RGD

positive regulation of cell migration involved in sprouting angiogenesis

Inferred from mutant phenotype PubMed 22752444. Source: RGD

positive regulation of cell-cell adhesion

Inferred from mutant phenotype PubMed 16622027. Source: RGD

positive regulation of cytosolic calcium ion concentration

Inferred from mutant phenotype PubMed 23999490. Source: RGD

positive regulation of fever generation

Inferred from mutant phenotype PubMed 15066130. Source: RGD

positive regulation of gene expression

Inferred from mutant phenotype PubMed 19559698PubMed 20940264. Source: RGD

positive regulation of inflammatory response

Inferred from mutant phenotype PubMed 20940264. Source: RGD

positive regulation of interleukin-1 beta secretion

Inferred from mutant phenotype PubMed 16790532. Source: RGD

positive regulation of interleukin-6 production

Inferred from mutant phenotype PubMed 16790532. Source: RGD

positive regulation of monocyte chemotaxis

Inferred from electronic annotation. Source: InterPro

positive regulation of neuron differentiation

Inferred from mutant phenotype PubMed 19559698. Source: RGD

positive regulation of tumor necrosis factor production

Inferred from mutant phenotype PubMed 16790532. Source: RGD

response to ionizing radiation

Inferred from expression pattern PubMed 16937495. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 19104678. Source: RGD

   Cellular_componentcell surface

Inferred from direct assay PubMed 16320322. Source: RGD

cytoplasm

Inferred from direct assay PubMed 16320322. Source: RGD

endosome

Inferred from direct assay PubMed 12004163. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionC-C chemokine receptor activity

Traceable author statement PubMed 12403770. Source: RGD

chemokine (C-C motif) ligand 5 binding

Inferred from mutant phenotype PubMed 22752444. Source: RGD

protein binding

Inferred from physical interaction PubMed 14674010PubMed 19559698. Source: RGD

protein kinase binding

Inferred from physical interaction PubMed 14674010. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354C-C chemokine receptor type 5
PRO_0000069280

Regions

Topological domain1 – 3232Extracellular Potential
Transmembrane33 – 6028Helical; Name=1; Potential
Topological domain61 – 7010Cytoplasmic Potential
Transmembrane71 – 9121Helical; Name=2; Potential
Topological domain92 – 10413Extracellular Potential
Transmembrane105 – 12622Helical; Name=3; Potential
Topological domain127 – 14317Cytoplasmic Potential
Transmembrane144 – 16825Helical; Name=4; Potential
Topological domain169 – 20032Extracellular Potential
Transmembrane201 – 22020Helical; Name=5; Potential
Topological domain221 – 23717Cytoplasmic Potential
Transmembrane238 – 26225Helical; Name=6; Potential
Topological domain263 – 27917Extracellular Potential
Transmembrane280 – 30324Helical; Name=7; Potential
Topological domain304 – 35451Cytoplasmic Potential

Amino acid modifications

Modified residue101Sulfotyrosine Potential
Modified residue161Sulfotyrosine Potential
Modified residue3381Phosphoserine; by BARK1 By similarity
Modified residue3391Phosphoserine; by BARK1 By similarity
Modified residue3441Phosphoserine; by BARK1 By similarity
Modified residue3511Phosphoserine; by BARK1 By similarity
Lipidation3231S-palmitoyl cysteine By similarity
Lipidation3261S-palmitoyl cysteine By similarity
Glycosylation61O-linked (GalNAc...) By similarity
Glycosylation191O-linked (GalNAc...) Potential
Disulfide bond103 ↔ 180 By similarity

Sequences

Sequence LengthMass (Da)Tools
O08556 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 77EDB368AA4C868D

FASTA35441,031
        10         20         30         40         50         60 
MDFQGSIPTY IYDIDYSMSA PCQKVNVKQI AAQLLPPLYS LVFIFGFVGN MMVFLILISC 

        70         80         90        100        110        120 
KKLKSMTDIY LFNLAISDLL FLLTLPFWAH YAANEWVFGN IMCKLFTGIY HIGYFGGIFF 

       130        140        150        160        170        180 
IILLTIDRYL AIVHAVFAIK ARTVNFGVIT SVVTWVVAVF VSLPEIIFMR SQKEGSHYTC 

       190        200        210        220        230        240 
SPHFLHIQYR FWKHFQTLKM VILSLILPLL VMVICYSGIL NTLFRCRNEK KRHRAVRLIF 

       250        260        270        280        290        300 
AIMIVYFLFW TPYNIVLLLT TFQEYFGLNN CSSSNRLDQA MQVTETLGMT HCCLNPVIYA 

       310        320        330        340        350 
FVGEKFRNYL SVFFRKHIVK RFCKHCSIFQ QVNPDRVSSV YTRSTGEQEV STGL 

« Hide

References

[1]"Cloning of rat HIV-1-chemokine coreceptor CKR5 from microglia and upregulation of its mRNA in ischemic and endotoxinemic rat brain."
Spleiss O., Gourmala N., Boddeke H.W.G.M., Sauter A., Fiebich B.L., Berger M., Gebicke-Haerter P.J.
J. Neurosci. Res. 53:16-28(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
[2]"Chemokine receptor expression in cultured glia and rat experimental allergic encephalomyelitis."
Jiang Y., Salafranca M.N., Adhikari S., Xia Y., Feng L., Sonntag M.K., deFiebre C.M., Pennell N.A., Streit W.J., Harrison J.K.
J. Neuroimmunol. 86:1-12(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12009 mRNA. Translation: CAA72737.1.
U77350 Genomic DNA. Translation: AAC03243.1.
RefSeqNP_446412.2. NM_053960.3.
UniGeneRn.10736.

3D structure databases

ProteinModelPortalO08556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000008871.

Chemistry

BindingDBO08556.
ChEMBLCHEMBL1795146.
GuidetoPHARMACOLOGY62.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO08556.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID117029.
KEGGrno:117029.
UCSCRGD:620596. rat.

Organism-specific databases

CTD1234.
RGD620596. Ccr5.

Phylogenomic databases

eggNOGNOG148353.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidO08556.
KOK04180.
PhylomeDBO08556.

Gene expression databases

GenevestigatorO08556.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002240. Chemokine_CCR5.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR01110. CHEMOKINER5.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619809.
PROO08556.

Entry information

Entry nameCCR5_RAT
AccessionPrimary (citable) accession number: O08556
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries