O08547 (SC22B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-trafficking protein SEC22b

Alternative name(s):
ER-Golgi SNARE of 24 kDa
Short name=ERS-24
Short name=ERS24
SEC22 vesicle-trafficking protein homolog B
SEC22 vesicle-trafficking protein-like 1
Short name=mSec22b

Gene names

Name:	Sec22b
Synonyms:	Sec22l1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
Subunit structure	Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex By similarity.
Subcellular location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Melanosome By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Traceable author statement. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum membrane Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum-Golgi intermediate compartment membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 215

214

Vesicle-trafficking protein SEC22b

PRO_0000206771

Regions

Topological domain

2 – 194

193

Cytoplasmic Potential

Transmembrane

195 – 215

21

Helical; Anchor for type IV membrane protein; Potential

Domain

6 – 119

114

Longin

Domain

134 – 194

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

35

1

Phosphoserine By similarity

Modified residue

38

1

N6-acetyllysine By similarity

Modified residue

48

1

Phosphoserine By similarity

Modified residue

137

1

Phosphoserine Ref.5 Ref.7 Ref.8 Ref.9

Modified residue

168

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

189

1

M → I in AAH09024. Ref.3

Secondary structure

1

.

.

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.

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215

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Turn

10 – 12

3

Beta strand

15 – 19

5

Helix

27 – 41

15

Beta strand

50 – 56

7

Beta strand

59 – 66

8

Beta strand

69 – 76

8

Helix

81 – 99

19

Turn

100 – 105

6

Turn

109 – 112

4

Helix

113 – 115

3

Helix

116 – 126

11

Sequences

	Sequence		Length	Mass (Da)
	O08547 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 29B4C55961C5A044	FASTA	215	24,741
	10 20 30 40 50 60 MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT RCTLEAGAMT 70 80 90 100 110 120 FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG KKVPTVSRPY SFIEFDTFIQ 130 140 150 160 170 180 KTKKLYIDSR ARRNLGSINT ELQDVQRIMV ANIEEVLQRG EALSALDSKA NNLSSLSKKY 190 200 210 RQDAKYLNMR STYAKLAAVA VFFIMLIVYV RFWWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells." Hay J.C., Chao D.S., Kuo C.S., Scheller R.H. Cell 89:149-158(1997) [PubMed: 9094723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Placenta.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Embryo, Pituitary and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-38, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY. Tissue: Brain cortex.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY. Tissue: Melanoma.
[9]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY. Tissue: Macrophage.
[10]	"A novel SNARE N-terminal domain revealed by the crystal structure of Sec22b." Gonzalez L.C. Jr., Weis W.I., Scheller R.H. J. Biol. Chem. 276:24203-24211(2001) [PubMed: 11309394] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-130.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U91538 mRNA. Translation: AAC53130.1.
AK017237 mRNA. Translation: BAB30646.1.
AK034973 mRNA. Translation: BAC28899.1.
AK037918 mRNA. Translation: BAC29901.1.
AK088514 mRNA. Translation: BAC40397.1.
AK089928 mRNA. Translation: BAC40999.1.
BC009024 mRNA. Translation: AAH09024.1.

IPI

IPI00114368.

RefSeq

NP_035472.1. NM_011342.4.

UniGene

Mm.2551.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IFQ	X-ray	2.40	A/B	2-127	[»]

ProteinModelPortal

O08547.

SMR

O08547. Positions 1-214.

ModBase

Search...

Protein-protein interaction databases

STRING

O08547.

PTM databases

PhosphoSite

O08547.

Proteomic databases

PRIDE

O08547.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879.

GeneID

20333.

KEGG

mmu:20333.

UCSC

uc008qpj.1. mouse.

Organism-specific databases

CTD

9554.

MGI

MGI:1338759. Sec22b.

Phylogenomic databases

eggNOG

maNOG18150.

GeneTree

ENSGT00510000046833.

HOGENOM

HBG327843.

HOVERGEN

HBG052748.

InParanoid

O08547.

OMA

QRIMVQN.

OrthoDB

EOG4XPQGX.

PhylomeDB

O08547.

Gene expression databases

ArrayExpress

O08547.

Bgee

O08547.

Genevestigator

O08547.

GermOnline

ENSMUSG00000027879. Mus musculus.

Family and domain databases

InterPro

IPR010908. Longin.
IPR011012. Longin-like.
IPR001388. Synaptobrevin.
[Graphical view]

Gene3D

G3DSA:3.30.450.50. Longin. 1 hit.

KO

K08517.

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PRINTS

PR00219. SYNAPTOBREVN.

SUPFAM

SSF64356. Longin_like. 1 hit.

PROSITE

PS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

298135.

SOURCE

Search...

Entry information

Entry name

SC22B_MOUSE

Accession

Primary (citable) accession number: O08547
Secondary accession number(s): Q91VU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program