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Protein

Vesicle-trafficking protein SEC22b

Gene

Sec22b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-trafficking protein SEC22b
Alternative name(s):
ER-Golgi SNARE of 24 kDa
Short name:
ERS-24
Short name:
ERS24
SEC22 vesicle-trafficking protein homolog B
SEC22 vesicle-trafficking protein-like 1
Short name:
mSec22b
Gene namesi
Name:Sec22b
Synonyms:Sec22l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1338759. Sec22b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 194CytoplasmicSequence analysisAdd BLAST193
Transmembranei195 – 215Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002067712 – 215Vesicle-trafficking protein SEC22bAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38N6-acetyllysineBy similarity1
Modified residuei137PhosphoserineCombined sources1
Modified residuei140PhosphothreonineBy similarity1
Modified residuei164PhosphoserineBy similarity1
Modified residuei168PhosphoserineCombined sources1
Modified residuei174PhosphoserineBy similarity1
Modified residuei177PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO08547.
MaxQBiO08547.
PaxDbiO08547.
PeptideAtlasiO08547.
PRIDEiO08547.
TopDownProteomicsiO08547.

PTM databases

iPTMnetiO08547.
PhosphoSitePlusiO08547.
SwissPalmiO08547.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027879.
ExpressionAtlasiO08547. baseline and differential.
GenevisibleiO08547. MM.

Interactioni

Subunit structurei

Interacts with STX17. Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203149. 3 interactors.
DIPiDIP-60852N.
IntActiO08547. 1 interactor.
MINTiMINT-1865345.
STRINGi10090.ENSMUSP00000029476.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Turni10 – 12Combined sources3
Beta strandi15 – 19Combined sources5
Helixi27 – 41Combined sources15
Beta strandi50 – 56Combined sources7
Beta strandi59 – 66Combined sources8
Beta strandi69 – 76Combined sources8
Helixi81 – 99Combined sources19
Turni100 – 105Combined sources6
Turni109 – 112Combined sources4
Helixi113 – 115Combined sources3
Helixi116 – 126Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IFQX-ray2.40A/B2-127[»]
ProteinModelPortaliO08547.
SMRiO08547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08547.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 119LonginPROSITE-ProRule annotationAdd BLAST114
Domaini134 – 194v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0862. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000046833.
HOGENOMiHOG000044957.
HOVERGENiHBG052748.
InParanoidiO08547.
KOiK08517.
OMAiVLYFWVL.
OrthoDBiEOG091G0MPE.
PhylomeDBiO08547.
TreeFamiTF105933.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT
60 70 80 90 100
RCTLEAGAMT FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG
110 120 130 140 150
KKVPTVSRPY SFIEFDTFIQ KTKKLYIDSR ARRNLGSINT ELQDVQRIMV
160 170 180 190 200
ANIEEVLQRG EALSALDSKA NNLSSLSKKY RQDAKYLNMR STYAKLAAVA
210
VFFIMLIVYV RFWWL
Length:215
Mass (Da):24,741
Last modified:January 23, 2007 - v3
Checksum:i29B4C55961C5A044
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189M → I in AAH09024 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91538 mRNA. Translation: AAC53130.1.
AK017237 mRNA. Translation: BAB30646.1.
AK034973 mRNA. Translation: BAC28899.1.
AK037918 mRNA. Translation: BAC29901.1.
AK088514 mRNA. Translation: BAC40397.1.
AK089928 mRNA. Translation: BAC40999.1.
BC009024 mRNA. Translation: AAH09024.1.
CCDSiCCDS17658.1.
RefSeqiNP_035472.1. NM_011342.4.
UniGeneiMm.2551.
Mm.486379.

Genome annotation databases

EnsembliENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879.
GeneIDi20333.
KEGGimmu:20333.
UCSCiuc008qpk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91538 mRNA. Translation: AAC53130.1.
AK017237 mRNA. Translation: BAB30646.1.
AK034973 mRNA. Translation: BAC28899.1.
AK037918 mRNA. Translation: BAC29901.1.
AK088514 mRNA. Translation: BAC40397.1.
AK089928 mRNA. Translation: BAC40999.1.
BC009024 mRNA. Translation: AAH09024.1.
CCDSiCCDS17658.1.
RefSeqiNP_035472.1. NM_011342.4.
UniGeneiMm.2551.
Mm.486379.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IFQX-ray2.40A/B2-127[»]
ProteinModelPortaliO08547.
SMRiO08547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203149. 3 interactors.
DIPiDIP-60852N.
IntActiO08547. 1 interactor.
MINTiMINT-1865345.
STRINGi10090.ENSMUSP00000029476.

PTM databases

iPTMnetiO08547.
PhosphoSitePlusiO08547.
SwissPalmiO08547.

Proteomic databases

EPDiO08547.
MaxQBiO08547.
PaxDbiO08547.
PeptideAtlasiO08547.
PRIDEiO08547.
TopDownProteomicsiO08547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879.
GeneIDi20333.
KEGGimmu:20333.
UCSCiuc008qpk.1. mouse.

Organism-specific databases

CTDi9554.
MGIiMGI:1338759. Sec22b.

Phylogenomic databases

eggNOGiKOG0862. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000046833.
HOGENOMiHOG000044957.
HOVERGENiHBG052748.
InParanoidiO08547.
KOiK08517.
OMAiVLYFWVL.
OrthoDBiEOG091G0MPE.
PhylomeDBiO08547.
TreeFamiTF105933.

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

EvolutionaryTraceiO08547.
PROiO08547.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027879.
ExpressionAtlasiO08547. baseline and differential.
GenevisibleiO08547. MM.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC22B_MOUSE
AccessioniPrimary (citable) accession number: O08547
Secondary accession number(s): Q91VU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.