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Protein

Ephrin-A3

Gene

Efna3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.1 Publication

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_280640. EPHA-mediated growth cone collapse.
REACT_292566. EPH-Ephrin signaling.
REACT_314615. EPH-ephrin mediated repulsion of cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A3
Alternative name(s):
EHK1 ligand
Short name:
EHK1-L
EPH-related receptor tyrosine kinase ligand 3
Short name:
LERK-3
Gene namesi
Name:Efna3
Synonyms:Epl3, Eplg3, Lerk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:106644. Efna3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 206184Ephrin-A3PRO_0000008371Add
BLAST
Propeptidei207 – 23024Removed in mature formSequence AnalysisPRO_0000008372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)1 Publication
Disulfide bondi63 ↔ 102PROSITE-ProRule annotation
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi91 ↔ 150PROSITE-ProRule annotation
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Lipidationi206 – 2061GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO08545.
PRIDEiO08545.

Expressioni

Gene expression databases

BgeeiO08545.
CleanExiMM_EFNA3.
ExpressionAtlasiO08545. baseline and differential.
GenevestigatoriO08545.

Interactioni

Subunit structurei

Interacts with EPHA8; activates EPHA8.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO08545.
SMRiO08545. Positions 28-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 161132Ephrin RBDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation
Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG295575.
GeneTreeiENSGT00390000015107.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiO08545.
KOiK05462.
OMAiGPHVKIN.
OrthoDBiEOG70W3FD.
PhylomeDBiO08545.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAPLLLLL LLVPVPLLPL LAQGPGGALG NRHAVYWNSS NQHLRREGYT
60 70 80 90 100
VQVNVNDYLD IYCPHYNSSG PGGGAEQYVL YMVNLSGYRT CNASQGSKRW
110 120 130 140 150
ECNRQHASHS PIKFSEKFQR YSAFSLGYEF HAGQEYYYIS TPTHNLHWKC
160 170 180 190 200
LRMKVFVCCA STSHSGEKPV PTLPQFTMGP NVKINVLEDF EGENPQVPKL
210 220 230
EKSISGTSPK REHLPLAVGI AFFLMTLLAS
Length:230
Mass (Da):25,632
Last modified:January 9, 2007 - v4
Checksum:iC08AAA0D84B0D795
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621Y → I in AAB50241 (PubMed:8903354).Curated
Sequence conflicti76 – 772EQ → DR in AAB50241 (PubMed:8903354).Curated
Sequence conflicti89 – 902RT → QP in AAB50241 (PubMed:8903354).Curated
Sequence conflicti121 – 1211Y → W in AAB50241 (PubMed:8903354).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107002 mRNA. Translation: AAI07003.1.
BC125003 mRNA. Translation: AAI25004.1.
U92885 Genomic DNA. Translation: AAC39961.1.
U90666 mRNA. Translation: AAB50241.1.
CCDSiCCDS38488.1.
RefSeqiNP_034238.1. NM_010108.1.
UniGeneiMm.331159.

Genome annotation databases

EnsembliENSMUST00000029673; ENSMUSP00000029673; ENSMUSG00000028039.
GeneIDi13638.
KEGGimmu:13638.
UCSCiuc008pyq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107002 mRNA. Translation: AAI07003.1.
BC125003 mRNA. Translation: AAI25004.1.
U92885 Genomic DNA. Translation: AAC39961.1.
U90666 mRNA. Translation: AAB50241.1.
CCDSiCCDS38488.1.
RefSeqiNP_034238.1. NM_010108.1.
UniGeneiMm.331159.

3D structure databases

ProteinModelPortaliO08545.
SMRiO08545. Positions 28-157.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiO08545.
PRIDEiO08545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029673; ENSMUSP00000029673; ENSMUSG00000028039.
GeneIDi13638.
KEGGimmu:13638.
UCSCiuc008pyq.1. mouse.

Organism-specific databases

CTDi1944.
MGIiMGI:106644. Efna3.

Phylogenomic databases

eggNOGiNOG295575.
GeneTreeiENSGT00390000015107.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiO08545.
KOiK05462.
OMAiGPHVKIN.
OrthoDBiEOG70W3FD.
PhylomeDBiO08545.

Enzyme and pathway databases

ReactomeiREACT_280640. EPHA-mediated growth cone collapse.
REACT_292566. EPH-Ephrin signaling.
REACT_314615. EPH-ephrin mediated repulsion of cells.

Miscellaneous databases

NextBioi284328.
PROiO08545.
SOURCEiSearch...

Gene expression databases

BgeeiO08545.
CleanExiMM_EFNA3.
ExpressionAtlasiO08545. baseline and differential.
GenevestigatoriO08545.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Characterization of the genes for mouse LERK-3/Ephrin-A3 (Epl3), mouse LERK-4/Ephrin-A4 (Epl4), and human LERK-6/Ephrin-A2 (EPLG6): conservation of intron/exon structure."
    Cerretti D.P., Nelson N.
    Genomics 47:131-135(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 44-230.
    Strain: 129.
  3. "Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis."
    Flenniken A.M., Gale N.W., Yancopoulos G.D., Wilkinson D.G.
    Dev. Biol. 179:382-401(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-121.
  4. "The Eek receptor, a member of the Eph family of tyrosine protein kinases, can be activated by three different Eph family ligands."
    Park S., Sanchez M.P.
    Oncogene 14:533-542(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EPHA8 ACTIVATION, INTERACTION WITH EPHA8.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-38.

Entry informationi

Entry nameiEFNA3_MOUSE
AccessioniPrimary (citable) accession number: O08545
Secondary accession number(s): O55217, Q08AV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2007
Last modified: April 1, 2015
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.