O08545 (EFNA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ephrin-A3

Alternative name(s):
EHK1 ligand
Short name=EHK1-L
EPH-related receptor tyrosine kinase ligand 3
Short name=LERK-3

Gene names

Name:	Efna3
Synonyms:	Epl3, Eplg3, Lerk3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Ref.4
Subunit structure	Interacts with EPHA8; activates EPHA8. Ref.4
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Belongs to the ephrin family. Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ephrin receptor signaling pathway Inferred from direct assay Ref.4. Source: UniProtKB
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 206	184	Ephrin-A3		PRO_0000008371
	Propeptide	207 – 230	24	Removed in mature form Potential		PRO_0000008372
Regions
	Domain	30 – 161	132	Ephrin RBD
Amino acid modifications
	Lipidation	206	1	GPI-anchor amidated glycine Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Disulfide bond	63 ↔ 102		By similarity
	Disulfide bond	91 ↔ 150		By similarity
Experimental info
	Sequence conflict	62	1	Y → I in AAB50241. Ref.3
	Sequence conflict	76 – 77	2	EQ → DR in AAB50241. Ref.3
	Sequence conflict	89 – 90	2	RT → QP in AAB50241. Ref.3
	Sequence conflict	121	1	Y → W in AAB50241. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O08545 [UniParc]. Last modified January 9, 2007. Version 4. Checksum: C08AAA0D84B0D795	FASTA	230	25,632
	10 20 30 40 50 60 MAAAPLLLLL LLVPVPLLPL LAQGPGGALG NRHAVYWNSS NQHLRREGYT VQVNVNDYLD 70 80 90 100 110 120 IYCPHYNSSG PGGGAEQYVL YMVNLSGYRT CNASQGSKRW ECNRQHASHS PIKFSEKFQR 130 140 150 160 170 180 YSAFSLGYEF HAGQEYYYIS TPTHNLHWKC LRMKVFVCCA STSHSGEKPV PTLPQFTMGP 190 200 210 220 230 NVKINVLEDF EGENPQVPKL EKSISGTSPK REHLPLAVGI AFFLMTLLAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Characterization of the genes for mouse LERK-3/Ephrin-A3 (Epl3), mouse LERK-4/Ephrin-A4 (Epl4), and human LERK-6/Ephrin-A2 (EPLG6): conservation of intron/exon structure." Cerretti D.P., Nelson N. Genomics 47:131-135(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 44-230. Strain: 129.
[3]	"Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis." Flenniken A.M., Gale N.W., Yancopoulos G.D., Wilkinson D.G. Dev. Biol. 179:382-401(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-121.
[4]	"The Eek receptor, a member of the Eph family of tyrosine protein kinases, can be activated by three different Eph family ligands." Park S., Sanchez M.P. Oncogene 14:533-542(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN EPHA8 ACTIVATION, INTERACTION WITH EPHA8.
[5]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-38, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC107002 mRNA. Translation: AAI07003.1. BC125003 mRNA. Translation: AAI25004.1. U92885 Genomic DNA. Translation: AAC39961.1. U90666 mRNA. Translation: AAB50241.1.
IPI	IPI00350390.
RefSeq	NP_034238.1. NM_010108.1.
UniGene	Mm.331159.
3D structure databases
ProteinModelPortal	O08545.
SMR	O08545. Positions 29-157.
ModBase	Search...
Proteomic databases
PRIDE	O08545.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029673; ENSMUSP00000029673; ENSMUSG00000028039.
GeneID	13638.
KEGG	mmu:13638.
UCSC	uc008pyq.1. mouse.
Organism-specific databases
CTD	1944.
MGI	MGI:106644. Efna3.
Phylogenomic databases
eggNOG	NOG295575.
GeneTree	ENSGT00390000015107.
HOGENOM	HOG000234373.
HOVERGEN	HBG051447.
InParanoid	O08545.
KO	K05462.
OMA	AGQEYYY.
OrthoDB	EOG4GB77B.
Gene expression databases
ArrayExpress	O08545.
Bgee	O08545.
CleanEx	MM_EFNA3.
Genevestigator	O08545.
GermOnline	ENSMUSG00000028039. Mus musculus.
Family and domain databases
Gene3D	2.60.40.420. 1 hit.
InterPro	IPR008972. Cupredoxin. IPR001799. Ephrin. IPR019765. Ephrin_CS. [Graphical view]
PANTHER	PTHR11304. PTHR11304. 1 hit.
Pfam	PF00812. Ephrin. 1 hit. [Graphical view]
PRINTS	PR01347. EPHRIN.
ProDom	PD002533. Ephrin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS01299. EPHRIN_RBD_1. 1 hit. PS51551. EPHRIN_RBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284328.
SOURCE	Search...

Entry information

Entry name

EFNA3_MOUSE

Accession

Primary (citable) accession number: O08545
Secondary accession number(s): O55217, Q08AV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program