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O08545 (EFNA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin-A3
Alternative name(s):
EHK1 ligand
Short name=EHK1-L
EPH-related receptor tyrosine kinase ligand 3
Short name=LERK-3
Gene names
Name:Efna3
Synonyms:Epl3, Eplg3, Lerk3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Ref.4

Subunit structure

Interacts with EPHA8; activates EPHA8. Ref.4

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Belongs to the ephrin family.

Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 206184Ephrin-A3
PRO_0000008371
Propeptide207 – 23024Removed in mature form Potential
PRO_0000008372

Regions

Domain30 – 161132Ephrin RBD

Amino acid modifications

Lipidation2061GPI-anchor amidated glycine Potential
Glycosylation381N-linked (GlcNAc...) Ref.5
Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Disulfide bond63 ↔ 102 By similarity
Disulfide bond91 ↔ 150 By similarity

Experimental info

Sequence conflict621Y → I in AAB50241. Ref.3
Sequence conflict76 – 772EQ → DR in AAB50241. Ref.3
Sequence conflict89 – 902RT → QP in AAB50241. Ref.3
Sequence conflict1211Y → W in AAB50241. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O08545 [UniParc].

Last modified January 9, 2007. Version 4.
Checksum: C08AAA0D84B0D795

FASTA23025,632
        10         20         30         40         50         60 
MAAAPLLLLL LLVPVPLLPL LAQGPGGALG NRHAVYWNSS NQHLRREGYT VQVNVNDYLD 

        70         80         90        100        110        120 
IYCPHYNSSG PGGGAEQYVL YMVNLSGYRT CNASQGSKRW ECNRQHASHS PIKFSEKFQR 

       130        140        150        160        170        180 
YSAFSLGYEF HAGQEYYYIS TPTHNLHWKC LRMKVFVCCA STSHSGEKPV PTLPQFTMGP 

       190        200        210        220        230 
NVKINVLEDF EGENPQVPKL EKSISGTSPK REHLPLAVGI AFFLMTLLAS 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Characterization of the genes for mouse LERK-3/Ephrin-A3 (Epl3), mouse LERK-4/Ephrin-A4 (Epl4), and human LERK-6/Ephrin-A2 (EPLG6): conservation of intron/exon structure."
Cerretti D.P., Nelson N.
Genomics 47:131-135(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 44-230.
Strain: 129.
[3]"Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis."
Flenniken A.M., Gale N.W., Yancopoulos G.D., Wilkinson D.G.
Dev. Biol. 179:382-401(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 60-121.
[4]"The Eek receptor, a member of the Eph family of tyrosine protein kinases, can be activated by three different Eph family ligands."
Park S., Sanchez M.P.
Oncogene 14:533-542(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN EPHA8 ACTIVATION, INTERACTION WITH EPHA8.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-38.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC107002 mRNA. Translation: AAI07003.1.
BC125003 mRNA. Translation: AAI25004.1.
U92885 Genomic DNA. Translation: AAC39961.1.
U90666 mRNA. Translation: AAB50241.1.
CCDSCCDS38488.1.
RefSeqNP_034238.1. NM_010108.1.
UniGeneMm.331159.

3D structure databases

ProteinModelPortalO08545.
SMRO08545. Positions 31-157.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO08545.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029673; ENSMUSP00000029673; ENSMUSG00000028039.
GeneID13638.
KEGGmmu:13638.
UCSCuc008pyq.1. mouse.

Organism-specific databases

CTD1944.
MGIMGI:106644. Efna3.

Phylogenomic databases

eggNOGNOG295575.
GeneTreeENSGT00390000015107.
HOGENOMHOG000234373.
HOVERGENHBG051447.
InParanoidO08545.
KOK05462.
OMAGPHVKIN.
OrthoDBEOG70W3FD.
PhylomeDBO08545.

Gene expression databases

ArrayExpressO08545.
BgeeO08545.
CleanExMM_EFNA3.
GenevestigatorO08545.

Family and domain databases

Gene3D2.60.40.420. 1 hit.
InterProIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERPTHR11304. PTHR11304. 1 hit.
PfamPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSPR01347. EPHRIN.
ProDomPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF49503. SSF49503. 1 hit.
PROSITEPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284328.
PROO08545.
SOURCESearch...

Entry information

Entry nameEFNA3_MOUSE
AccessionPrimary (citable) accession number: O08545
Secondary accession number(s): O55217, Q08AV0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot