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Protein

Myc box-dependent-interacting protein 1

Gene

Bin1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

GO - Molecular functioni

  • actin filament binding Source: MGI
  • RNA polymerase binding Source: MGI
  • tau protein binding Source: Alzheimers_University_of_Toronto

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • lipid tube assembly Source: Alzheimers_University_of_Toronto
  • muscle cell differentiation Source: MGI
  • nucleus localization Source: WormBase
  • nucleus organization Source: MGI
  • positive regulation of apoptotic process Source: Alzheimers_University_of_Toronto
  • positive regulation of astrocyte differentiation Source: Alzheimers_University_of_Toronto
  • regulation of cell cycle arrest Source: Alzheimers_University_of_Toronto
  • regulation of endocytosis Source: InterPro
  • regulation of neuron differentiation Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Endocytosis

Enzyme and pathway databases

ReactomeiR-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Myc box-dependent-interacting protein 1
Alternative name(s):
Amphiphysin II
Amphiphysin-like protein
Bridging integrator 1
SH3 domain-containing protein 9
Gene namesi
Name:Bin1
Synonyms:Amphl, Sh3p9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:108092. Bin1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Alzheimers_University_of_Toronto
  • axon initial segment Source: Alzheimers_University_of_Toronto
  • cytoplasm Source: MGI
  • I band Source: Alzheimers_University_of_Toronto
  • lipid tube Source: Alzheimers_University_of_Toronto
  • membrane Source: MGI
  • microtubule Source: Alzheimers_University_of_Toronto
  • node of Ranvier Source: Alzheimers_University_of_Toronto
  • nuclear envelope Source: WormBase
  • nucleus Source: MGI
  • T-tubule Source: Alzheimers_University_of_Toronto
  • Z disc Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001929522 – 588Myc box-dependent-interacting protein 1Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei296PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by protein kinase C.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO08539.
PaxDbiO08539.
PeptideAtlasiO08539.
PRIDEiO08539.

PTM databases

iPTMnetiO08539.
PhosphoSitePlusiO08539.

Miscellaneous databases

PMAP-CutDBO08539.

Expressioni

Tissue specificityi

Isoform 1 is expressed mainly in the brain. Isoform 2 is widely expressed.

Gene expression databases

BgeeiENSMUSG00000024381.
CleanExiMM_BIN1.
ExpressionAtlasiO08539. baseline and differential.
GenevisibleiO08539. MM.

Interactioni

Subunit structurei

Heterodimer with AMPH (By similarity). Interacts (via SH3 domain) with SYNJ1. Interacts (via SH3 domain) with DNM1. Interacts with CLTC. Interacts with AP2A2. Interacts with AP2B1. Interacts with MYC (via N-terminal transactivation domain); the interaction requires the integrity of the conserved MYC box regions 1 and 2. Binds SH3GLB1. Interacts with BIN2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MaptP106372EBI-775152,EBI-774043
Mtm1Q9Z2C53EBI-775152,EBI-6861578

GO - Molecular functioni

  • actin filament binding Source: MGI
  • RNA polymerase binding Source: MGI
  • tau protein binding Source: Alzheimers_University_of_Toronto

Protein-protein interaction databases

BioGridi206025. 8 interactors.
IntActiO08539. 10 interactors.
MINTiMINT-263281.
STRINGi10090.ENSMUSP00000025239.

Structurei

3D structure databases

ProteinModelPortaliO08539.
SMRiO08539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 276BARPROSITE-ProRule annotationAdd BLAST248
Domaini515 – 588SH3PROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 122Interaction with BIN2By similarityAdd BLAST121
Regioni379 – 422Clathrin-bindingBy similarityAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 42Sequence analysisAdd BLAST28
Coiled coili193 – 274Sequence analysisAdd BLAST82

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410ITR4. Eukaryota.
ENOG410XQXT. LUCA.
GeneTreeiENSGT00390000017588.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08539.
KOiK12562.
OMAiVYEPEWP.
OrthoDBiEOG091G07IE.
PhylomeDBiO08539.
TreeFamiTF313542.

Family and domain databases

Gene3Di1.20.1270.60. 2 hits.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003023. Amphiphysin_2.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01253. AMPHIPHYSIN2.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O08539-1) [UniParc]FASTAAdd to basket
Also known as: BRAMP2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN
60 70 80 90 100
FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLSECLQEVY EPEWPGRDEA
110 120 130 140 150
NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY
160 170 180 190 200
DSARHHYESL QTAKKKDEAK IAKPVSLLEK AAPQWCQGKL QAHLVAQTNL
210 220 230 240 250
LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE
260 270 280 290 300
NFHKEMSKLN QNLNDVLVSL EKQHGSNTFT VKAQPSDNAP EKGNKSPSPP
310 320 330 340 350
PDGSPAATPE IRVNHEPEPA SGASPGATIP KSPSQLRKGP PVPPPPKHTP
360 370 380 390 400
SKEMKQEQIL SLFDDAFVPE ISVTTPSQFE APGPFSEQAS LLDLDFEPLP
410 420 430 440 450
PVASPVKAPT PSGQSIPWDL WEPTESQAGI LPSGEPSSAE GSFAVAWPSQ
460 470 480 490 500
TAEPGPAQPA EASEVVGGAQ EPGETAASEA TSSSLPAVVV ETFSATVNGA
510 520 530 540 550
VEGSAGTGRL DLPPGFMFKV QAQHDYTATD TDELQLKAGD VVLVIPFQNP
560 570 580
EEQDEGWLMG VKESDWNQHK ELEKCRGVFP ENFTERVQ
Length:588
Mass (Da):64,470
Last modified:July 1, 1997 - v1
Checksum:i63CA362461500F38
GO
Isoform 2 (identifier: O08539-2) [UniParc]FASTAAdd to basket
Also known as: SH3P9

The sequence of this isoform differs from the canonical sequence as follows:
     174-204: Missing.
     335-457: Missing.

Show »
Length:434
Mass (Da):48,014
Checksum:iB0E861C793BB143A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000254174 – 204Missing in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_000255335 – 457Missing in isoform 2. 1 PublicationAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86405 mRNA. Translation: AAC53318.1.
U60884 mRNA. Translation: AAC52661.1.
CCDSiCCDS29119.1. [O08539-1]
RefSeqiNP_033798.1. NM_009668.2. [O08539-1]
XP_006526056.1. XM_006525993.3. [O08539-2]
UniGeneiMm.4383.

Genome annotation databases

EnsembliENSMUST00000025239; ENSMUSP00000025239; ENSMUSG00000024381. [O08539-1]
GeneIDi30948.
KEGGimmu:30948.
UCSCiuc008ejh.1. mouse. [O08539-1]
uc008ejj.1. mouse. [O08539-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86405 mRNA. Translation: AAC53318.1.
U60884 mRNA. Translation: AAC52661.1.
CCDSiCCDS29119.1. [O08539-1]
RefSeqiNP_033798.1. NM_009668.2. [O08539-1]
XP_006526056.1. XM_006525993.3. [O08539-2]
UniGeneiMm.4383.

3D structure databases

ProteinModelPortaliO08539.
SMRiO08539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206025. 8 interactors.
IntActiO08539. 10 interactors.
MINTiMINT-263281.
STRINGi10090.ENSMUSP00000025239.

PTM databases

iPTMnetiO08539.
PhosphoSitePlusiO08539.

Proteomic databases

MaxQBiO08539.
PaxDbiO08539.
PeptideAtlasiO08539.
PRIDEiO08539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025239; ENSMUSP00000025239; ENSMUSG00000024381. [O08539-1]
GeneIDi30948.
KEGGimmu:30948.
UCSCiuc008ejh.1. mouse. [O08539-1]
uc008ejj.1. mouse. [O08539-2]

Organism-specific databases

CTDi274.
MGIiMGI:108092. Bin1.

Phylogenomic databases

eggNOGiENOG410ITR4. Eukaryota.
ENOG410XQXT. LUCA.
GeneTreeiENSGT00390000017588.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiO08539.
KOiK12562.
OMAiVYEPEWP.
OrthoDBiEOG091G07IE.
PhylomeDBiO08539.
TreeFamiTF313542.

Enzyme and pathway databases

ReactomeiR-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiBin1. mouse.
PMAP-CutDBO08539.
PROiO08539.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024381.
CleanExiMM_BIN1.
ExpressionAtlasiO08539. baseline and differential.
GenevisibleiO08539. MM.

Family and domain databases

Gene3Di1.20.1270.60. 2 hits.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003023. Amphiphysin_2.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01253. AMPHIPHYSIN2.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIN1_MOUSE
AccessioniPrimary (citable) accession number: O08539
Secondary accession number(s): Q62434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.