Reviewed, UniProtKB/Swiss-Prot O08538 (ANGP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Angiopoietin-1
      Short name=ANG-1

Gene names

Name:	Angpt1
Synonyms:	Agpt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Subcellular location	Secreted.
Developmental stage	Early in development, at E9 to E11, it is found most prominently in the heart myocardium surrounding the endocardium. Later, it becomes more widely distributed, most often in the mesenchyme surrounding developing vessels, in close association with endothelial cells.
Sequence similarities	Contains 1 fibrinogen C-terminal domain.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Secreted
Domain	Coiled coil Signal
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW endoderm development Traceable author statement. Source: MGI transmembrane receptor protein tyrosine kinase signaling pathway Inferred from direct assay. Source: MGI
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	vascular endothelial growth factor receptor binding Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 498	479	Angiopoietin-1		PRO_0000009111
Regions
	Domain	277 – 497	221	Fibrinogen C-terminal
	Coiled coil	81 – 119	39	Potential
	Coiled coil	153 – 261	109	Potential
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Disulfide bond	286 ↔ 315		By similarity
	Disulfide bond	439 ↔ 452		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O08538-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 285B4FDEC260D800	FASTA	498	57,505
	10 20 30 40 50 60 MTVFLSFAFF AAILTHIGCS NQRRNPENGG RRYNRIQHGQ CAYTFILPEH DGNCRESATE 70 80 90 100 110 120 QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL ENYIVENMKS EMAQIQQNAV 130 140 150 160 170 180 QNHTATMLEI GTSLLSQTAE QTRKLTDVET QVLNQTSRLE IQLLENSLST YKLEKQLLQQ 190 200 210 220 230 240 TNEILKIHEK NSLLEHKILE MEGKHKEELD TLKEEKENLQ GLVSRQTFII QELEKQLSRA 250 260 270 280 290 300 TNNNSILQKQ QLELMDTVHN LVSLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY 310 320 330 340 350 360 TIYFNNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN PSGEYWLGNE 370 380 390 400 410 420 FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN YRLYLKGHTG TAGKQSSLIL 430 440 450 460 470 480 HGADFSTKDA DNDNCMCKCA LMLTGGWWFD ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK 490 GPSYSLRSTT MMIRPLDF

References

[1]	"Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." Davis S., Aldrich T.H., Jones P.F., Acheson A., Compton D.L., Jain V., Ryan T.E., Bruno J., Radziejewski C., Maisonpierre P.C., Yancopoulos G.D. Cell 87:1161-1169(1996) [PubMed: 8980223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U83509 mRNA. Translation: AAB50558.1.
IPI	IPI00114350.
UniGene	Mm.309336
3D structure databases
HSSP	HSSP built from PDB template 1FZD based on UniProtKB P02671.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6051N.
PTM databases
PhosphoSite	O08538.
Proteomic databases
PRIDE	O08538.
Genome annotation databases
Ensembl	ENSMUSG00000022309. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:108448. Angpt1.
Phylogenomic databases
HOVERGEN	O08538.
Gene expression databases
ArrayExpress	O08538.
Bgee	O08538.
CleanEx	MM_ANGPT1.
GermOnline	ENSMUSG00000022309. Mus musculus.
Family and domain databases
InterPro	IPR002181. Fibrinogen_a/b/g_C. IPR014716. Fibrinogen_a/b/g_C_1. [Graphical view]
Gene3D	G3DSA:3.90.215.10. Fibrinogen_a/b/g_C_1. 1 hit.
Pfam	PF00147. Fibrinogen_C. 1 hit. [Graphical view]
SMART	SM00186. FBG. 1 hit. [Graphical view]
PROSITE	PS00514. FIBRINOGEN_C_1. 1 hit. PS51406. FIBRINOGEN_C_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ANGP1_MOUSE

Accession

Primary (citable) accession number: O08538

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)