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O08538

- ANGP1_MOUSE

UniProt

O08538 - ANGP1_MOUSE

Protein

Angiopoietin-1

Gene

Angpt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme By similarity.By similarity

    GO - Molecular functioni

    1. receptor binding Source: MGI
    2. vascular endothelial growth factor receptor binding Source: MGI

    GO - Biological processi

    1. activation of transmembrane receptor protein tyrosine kinase activity Source: Ensembl
    2. angiogenesis Source: MGI
    3. cardiac muscle tissue morphogenesis Source: DFLAT
    4. cell-substrate adhesion Source: MGI
    5. endocardium morphogenesis Source: DFLAT
    6. endoderm development Source: MGI
    7. glomerulus vasculature development Source: MGI
    8. hemopoiesis Source: MGI
    9. heparin biosynthetic process Source: Ensembl
    10. in utero embryonic development Source: MGI
    11. negative regulation of cell adhesion Source: Ensembl
    12. negative regulation of endothelial cell apoptotic process Source: Ensembl
    13. negative regulation of neuron apoptotic process Source: MGI
    14. negative regulation of vascular permeability Source: MGI
    15. patterning of blood vessels Source: DFLAT
    16. positive chemotaxis Source: Ensembl
    17. positive regulation of blood vessel endothelial cell migration Source: Ensembl
    18. positive regulation of cell adhesion Source: MGI
    19. positive regulation of endothelial cell migration Source: DFLAT
    20. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    21. positive regulation of peptidyl-serine phosphorylation Source: MGI
    22. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
    23. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
    24. positive regulation of protein kinase B signaling Source: Ensembl
    25. positive regulation of protein ubiquitination Source: Ensembl
    26. positive regulation of receptor internalization Source: Ensembl
    27. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: DFLAT
    28. protein localization to cell surface Source: Ensembl
    29. regulation of satellite cell proliferation Source: Ensembl
    30. sprouting angiogenesis Source: Ensembl
    31. Tie signaling pathway Source: DFLAT
    32. transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
    33. vasculogenesis Source: DFLAT
    34. vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Differentiation

    Enzyme and pathway databases

    ReactomeiREACT_211860. Tie2 Signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiopoietin-1
    Short name:
    ANG-1
    Gene namesi
    Name:Angpt1
    Synonyms:Agpt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:108448. Angpt1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: Ensembl
    2. membrane raft Source: Ensembl
    3. microvillus Source: Ensembl
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Embryonically lethal. Embryos die at about 12.5 dpc, due to important developmental defects of the endocardium and myocardium, plus generalized defects in vascular development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 498479Angiopoietin-1PRO_0000009111Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi243 – 2431N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi286 ↔ 315PROSITE-ProRule annotation
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi439 ↔ 452PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO08538.
    PaxDbiO08538.
    PRIDEiO08538.

    PTM databases

    PhosphoSiteiO08538.

    Expressioni

    Developmental stagei

    Early in development, at E9 to E11, it is found most prominently in the heart myocardium surrounding the endocardium. Later, it becomes more widely distributed, most often in the mesenchyme surrounding developing vessels, in close association with endothelial cells.

    Gene expression databases

    ArrayExpressiO08538.
    BgeeiO08538.
    CleanExiMM_ANGPT1.
    GenevestigatoriO08538.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with TEK/TIE2 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-6051N.
    STRINGi10090.ENSMUSP00000022921.

    Structurei

    3D structure databases

    ProteinModelPortaliO08538.
    SMRiO08538. Positions 151-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini277 – 497221Fibrinogen C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili81 – 11939Sequence AnalysisAdd
    BLAST
    Coiled coili153 – 261109Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Signal

    Phylogenomic databases

    eggNOGiNOG310490.
    GeneTreeiENSGT00720000108441.
    HOVERGENiHBG001644.
    InParanoidiQ6NWV7.
    KOiK05465.
    OMAiTSQRQYS.
    OrthoDBiEOG7X9G60.
    TreeFamiTF336658.

    Family and domain databases

    Gene3Di3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProiIPR028843. Ang-1.
    IPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR020837. Fibrinogen_CS.
    [Graphical view]
    PANTHERiPTHR19143:SF156. PTHR19143:SF156. 1 hit.
    PfamiPF00147. Fibrinogen_C. 1 hit.
    [Graphical view]
    SMARTiSM00186. FBG. 1 hit.
    [Graphical view]
    SUPFAMiSSF56496. SSF56496. 1 hit.
    PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O08538-1 [UniParc]FASTAAdd to Basket

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    MTVFLSFAFF AAILTHIGCS NQRRNPENGG RRYNRIQHGQ CAYTFILPEH    50
    DGNCRESATE QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL 100
    ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET 150
    QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE 200
    MEGKHKEELD TLKEEKENLQ GLVSRQTFII QELEKQLSRA TNNNSILQKQ 250
    QLELMDTVHN LISLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY 300
    TIYFNNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN 350
    PSGEYWLGNE FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN 400
    YRLYLKGHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD 450
    ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK GPSYSLRSTT MMIRPLDF 498
    Length:498
    Mass (Da):57,519
    Last modified:July 27, 2011 - v2
    Checksum:iFC36F905A9E79074
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti262 – 2621I → V in AAB50558. (PubMed:8980223)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83509 mRNA. Translation: AAB50558.1.
    BC067410 mRNA. Translation: AAH67410.1.
    CCDSiCCDS27450.1.
    RefSeqiNP_033770.2. NM_009640.4.
    UniGeneiMm.309336.

    Genome annotation databases

    EnsembliENSMUST00000022921; ENSMUSP00000022921; ENSMUSG00000022309.
    GeneIDi11600.
    KEGGimmu:11600.
    UCSCiuc007vpc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83509 mRNA. Translation: AAB50558.1 .
    BC067410 mRNA. Translation: AAH67410.1 .
    CCDSi CCDS27450.1.
    RefSeqi NP_033770.2. NM_009640.4.
    UniGenei Mm.309336.

    3D structure databases

    ProteinModelPortali O08538.
    SMRi O08538. Positions 151-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6051N.
    STRINGi 10090.ENSMUSP00000022921.

    PTM databases

    PhosphoSitei O08538.

    Proteomic databases

    MaxQBi O08538.
    PaxDbi O08538.
    PRIDEi O08538.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022921 ; ENSMUSP00000022921 ; ENSMUSG00000022309 .
    GeneIDi 11600.
    KEGGi mmu:11600.
    UCSCi uc007vpc.1. mouse.

    Organism-specific databases

    CTDi 284.
    MGIi MGI:108448. Angpt1.

    Phylogenomic databases

    eggNOGi NOG310490.
    GeneTreei ENSGT00720000108441.
    HOVERGENi HBG001644.
    InParanoidi Q6NWV7.
    KOi K05465.
    OMAi TSQRQYS.
    OrthoDBi EOG7X9G60.
    TreeFami TF336658.

    Enzyme and pathway databases

    Reactomei REACT_211860. Tie2 Signaling.

    Miscellaneous databases

    ChiTaRSi ANGPT1. mouse.
    NextBioi 279116.
    PROi O08538.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O08538.
    Bgeei O08538.
    CleanExi MM_ANGPT1.
    Genevestigatori O08538.

    Family and domain databases

    Gene3Di 3.90.215.10. 1 hit.
    4.10.530.10. 1 hit.
    InterProi IPR028843. Ang-1.
    IPR014716. Fibrinogen_a/b/g_C_1.
    IPR014715. Fibrinogen_a/b/g_C_2.
    IPR002181. Fibrinogen_a/b/g_C_dom.
    IPR020837. Fibrinogen_CS.
    [Graphical view ]
    PANTHERi PTHR19143:SF156. PTHR19143:SF156. 1 hit.
    Pfami PF00147. Fibrinogen_C. 1 hit.
    [Graphical view ]
    SMARTi SM00186. FBG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56496. SSF56496. 1 hit.
    PROSITEi PS00514. FIBRINOGEN_C_1. 1 hit.
    PS51406. FIBRINOGEN_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning."
      Davis S., Aldrich T.H., Jones P.F., Acheson A., Compton D.L., Jain V., Ryan T.E., Bruno J., Radziejewski C., Maisonpierre P.C., Yancopoulos G.D.
      Cell 87:1161-1169(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis."
      Suri C., Jones P.F., Patan S., Bartunkova S., Maisonpierre P.C., Davis S., Sato T.N., Yancopoulos G.D.
      Cell 87:1171-1180(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiANGP1_MOUSE
    AccessioniPrimary (citable) accession number: O08538
    Secondary accession number(s): Q6NWV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3